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Eight new caffeoyl derivatives, elephantomentosides A–H (1 − 8), together with ten known ones (9 − 18), were isolated from the whole plant of Elephantopos tomentosus L. Their structures were elucidated using detailed spectroscopic analysis. Structurally, compounds 1 − 8 are composed of β-D-glucopyranose, and almost all of the substituent positions are at the C-1′ and C-4′ of glucopyranose. The anti-inflammatory and antioxidant activities of all isolated compounds were evaluated in vitro. Compounds 9–10, 13–15, and 17–18 exhibited significant DPPH scavenging capacity with IC50 values in the range of 10.01–25.07 μM, in comparison with Vc (IC50, 17.98 μM). [ABSTRACT FROM AUTHOR]

Nucleotides and their derivatives are vital biomarkers that play essential roles in biochemical reactions and influence processes of metabolism and anabolism. However, discriminating between various structurally similar nucleotides and their phosphate derivatives using convenient means still poses a challenge. Here, we designed a supramolecular fluorescence sensor array constructed with four different calixarenes loaded with dyes to discriminate between nucleotides and their phosphate derivatives. Based on the differences in the ability of calixarenes to recognise various nucleotides and nucleotide phosphates, a unique fluorescence response pattern is generated for each analyte. With the assistance of linear discriminant analysis, the discrimination of target analytes is effectively achieved. Subsequent assays involving various concentrations of nucleoside triphosphates and mixtures of nucleotide phosphates have also verified the potential of this sensor array based on calixarenes in the investigation of nucleotides. [ABSTRACT FROM AUTHOR]

With the development of sequencing technology, more and more rare thalassemia types have been found. In this article, we found a novel Hb H disease combined with glucose-6-phosphate dehydrogenase (G6PD) deficiency through whole genome sequencing (WGS), which was verified by Sanger sequencing and polymerase chain reaction (PCR)-reverse dot-blot hybridization, respectively. [ABSTRACT FROM AUTHOR]