Background and Objectives: Water is one of the main pillars of the country's sustainable development, and surface water resources play an undeniable role in this by providing many needs for drinking, industry and agriculture. These resources must meet different demands not only in terms of quantity but also in terms of quality. In most studies, complex watershed models that evaluate hydrology and water quality simultaneously are widely used (Borui and Gazzetti 2014, Haas et al. 2016), but these models are difficult to run on large amounts of data and information. They require input and this can be considered as a limitation for use in developing countries where we face a lack of quality data (Paiva et al. 2012, Melo et al. 2016). This limitation in the use of models can be overcome by using simpler qualitative-quantitative methods that require less data and are less dependent on pollutant flow and transfer equations, such as the Load Duration curves (LDC) method (Serrano et al. 2020). LDC is a graphical output obtained by combining daily flow values with water quality data and is used to assess pollutants (McKay et al. 2010). The LDC curve provides insight into the loading pattern under different flow conditions, particularly by characterizing point and nonpoint pollutant sources (Johnson et al. 2009). This technique is often used in the development of watershed protection plans as well as in TMDL calculations (Beynam 2007, Ward et al. 2009). The use of load Duraton curves (LDC) for water quality planning and management, especially to support TMDL programs, is common in different countries due to the need for less data compared to complex quality models. However, this method has been used in Iran. has not been In this research, by drawing the LDC and TMDL curves for the nitrate pollutant in the Tejn River, the qualitative condition of the river for agricultural and aquatic uses along the 80-kilometer route from the downstream of the Shahid Rajaei Dam to the mouth of the sea has been investigated. Methodology: In this research, by choosing two hydrometric stations, Rig Cheshme and Kordkheyl, along the flow path of the Tejn River, the Tejn watershed was divided into three sections with different characteristics. The upstream area of Rig Cheshme station, where the Shahid Rajaei Dam is located, mostly includes forest and mountain areas with natural vegetation and agricultural lands and less human settlements. The middle area includes the areas between Rig Cheshme station and Kordkheyl station, which mostly includes agricultural lands, especially rice fields, citrus and blackberry orchards, as well as numerous urban and rural areas, and the lower area, which includes the downstream areas from Kordkheyl station to the mouth of the sea. Investigating the quality conditions of the river flow path in these two stations can determine the perspective of nitrate pollution under the influence of these two land use patterns in the region. The data of the research includes the information of the daily discharge of the stream for 18 years (1998-1999 to 2016-2017) and the nitrate concentration measured for 8 years (2011-2012 to 2019-2020) at the location of the hydrometric stations, which were obtained from the Mazandaran Regional Water Company. In order to analyze the flow conditions and the rate of nitrate pollution in the water of Tajen river, the latest quantitative and qualitative long-term information was obtained from the regional water company of Mazandaran province and the average flow rate in the months of the year and the changes of nitrates in the basin were investigated. The 18-year daily flow data from the water year 1998-1999 to the water year 2016-2017 were used to draw the flow Duraton curve. Considering that the studied area mostly includes agricultural lands and as a result, the entry of pollutants from these lands is a function of the conditions of the cultivation and non-cultivation seasons. Therefore, the discharges are divided into two groups of discharges of the cultivation and non-cultivation seasons. Then for each of these seasons, the Flow Duraton curve was drawn. For this purpose, the discharges were arranged in descending order and using the Kolmogorov-Smirnov index of probability distribution function suitable for each data series, the probability of occurrence of each of the discharges was calculated. To investigate the quality conditions of Tajen river in terms of nitrate concentration, the data measured in 2011-2012 to 2016-2017 were used. After drawing the continuous Flow Duration curve (FDC), by multiplying the standard values of nitrate concentration by the flow rate, the maximum allowable daily nitrate load was obtained. Also, by multiplying the measured nitrate load with the corresponding flow rates, the amount of nitrate passing through was calculated. Investigation and determination of Total Maximum Daily Load (TMDL) curves for two agricultural uses and water ecosystems (environment) in two seasons of cultivation and non-cultivation were carried out at measuring stations along the flow path. By drawing the curves of TMDL and LDC values, investigating and analyzing the quality conditions of the river along about 80 kilometers between the Shahid Rajaei dam and the mouth of the river in terms of the amount of nitrate pollution with the aim of agricultural and aquaculture applications in terms of space and time, and the necessary implementation proposals presented. Findings: The discharge data series of Rig Cheshmeh station has a Normal distribution in the cultivation season and a Burr distribution in the non-cultivation season, and the data series in Kordkheyl station have a better fit with the Burr distribution in both the cultivation and non-cultivation seasons. The flow rate at Rig Cheshme station is higher in the first half of the water year and lower in the second half of the year. This is due to the withdrawal of water from Shahid Rajaei Dam in the second half and the release of more water for agricultural uses in the first half of the year. The discharge changes in Kordkheyl station have different conditions, so that in the first half of the year we see lower discharges and in the second half of the year more discharges. This issue is due to more harvests of river water for agriculture, especially rice cultivation in the river terrace lands, during spring and summer. The reason for the higher discharge in the second half of the year is the connection of the branches downstream of Rig Cheshme station. Flow Duraton curve (FDC) and discharges with a probability of 90% for Rig Cheshme and Kordkheyl stations and separated by cultivation and non-cultivation seasons. At Rig Cheshme station in discharges with a probability of 90%, it has more values than at Kordkheyl station, which is justified considering that Rig Cheshme station is located upstream and the withdrawals from the river are less in those areas. On the other hand, because there are limited agricultural lands upstream of Rig Cheshme station, and most of the lands are forest and pasture, therefore, due to the need of water during the cultivation period in the downstream; More water has been released from Shahid Rajaei Dam and higher discharges have occurred. On the other hand, due to the location of abundant agricultural lands and harvesting from the river in the cultivation season upstream of Kordkheyl station, the discharge values in this station are lower in the cultivation season than in the non-cultivation season. In the investigated period, in none of the data collection times, nitrate values (LDC) were not more than the permissible limit (TMDL) and therefore, from the upstream of the river to the location of Rig Cheshme station, favorable quality conditions were established in terms of nitrate pollutant concentration, and in terms of agricultural uses and There is no limit to aquaculture throughout the year. Carefully in the curves, it is determined that the values of the passing load in larger flows are closer to the TMDL curve, so that these points are in the range of 10-40% probability of occurrence and in wet conditions. In the LDC curve, if loads are in this range, their origin can be non-point pollution loads (Tiago et al. 2011, Chin 2012, Thiago 2012). Therefore, it can be concluded that the origin of loads in the upstream area of Rig Cheshme station are mostly non-point sources. according to LDC curves of Kordkheyl station, It can be seen that some of the values are tangent or above the TMDL curve, which indicates the violation of the rules of the maximum allowed load in this station. This has occurred in low river flows, indicating the impact of point pollutant sources (Cleland 2002, Bonta and Cleland 2003, Serrano et al. 2020). There are many point sources in the upstream area of Kordkheyl station and its distance from Rig Cheshme station. The location of three industrial centers of wood and paper factory, dairy products company and Sari antibiotic factory in this area introduce point pollutants to the river. Also, the effluents of the city of Sari and the neighboring villages are located in this area and enter the river as a point along the flow path (Sadeghi Azad 2017). The limitation of river water use occurs from medium flows to low flows (probabilities of occurrence (40-100%) which are related to the irrigation seasons of paddy fields. Conclusion: In this research, using LDC in two stations, Rig Cheshme and Kordkheyl, the quality conditions and factors affecting the water quality of Tajen River were investigated. Examining the trend of pollutant concentration changes in consecutive years shows that the Rig Cheshme station has almost a constant trend and the concentrations do not show much change, but in Kordkheyl station, over the years, the nitrate load concentration of the river, both in the cultivation season and in the non-cultivation season, cultivation has been added. The results showed that most of the non-point sources are effective on the nitrate pollution of the river in the area of Rig Cheshme station. But these factors do not create a situation where the nitrate concentration in the river exceeds the maximum limit. The results indicate that the Load Duraton curves compared to the Total Maximum Daily Load for agricultural, fisheries and aquaculture applications from the upstream of Shahid Rajaei Dam to Rig Cheshme Station are in the safe margin and have no limitations. Examining the graphs of the Load Duraton curve for river at Kordkheyl station indicates that the amount of nitrate pollutant has increased from the maximum allowed, and these cases are related to the minimum discharges and during the cultivation seasons. On the other hand, according to the surveys conducted and according to the quality conditions of the river at Kordkheyl station up to 16 km below (the mouth of the Tajen River to the Caspian Sea), during the cultivation seasons, the river will suffer nitrate pollution more than the allowed limit. Therefore, it is necessary to revise the calculations of releasing the flow from Shahid Rajaei Dam. Also, the management of the use of nitrogen fertilizers in the fields and gardens of the region, especially the edges of the flow path from Kordkheyl station to the mouth of the sea, has been reviewed and reconsidered. [ABSTRACT FROM AUTHOR]