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A regioselective synthesis of 4H‐pyrano[2,3‐b]quinoline derivatives via DABCO‐mediated Knoevenagel condensation/heterocyclization sequence has been executed. In this way some new fused heterocyclic compounds were synthesized from 2‐chloroquinoline‐3‐carbaldehyde as a versatile and efficient synthetic building block. The one‐pot three‐component reaction of 2‐chloroquinoline‐3‐carbaldehyde and diverse cyclic active methylenes for construction of highly substituted quinolines scaffold has been accomplished under mild condition. The strategy included herein shows significant advantages, including a facile process with easy purification, excellent yields, wide applicability, available substrates, and cost‐effective/eco‐friendly solvent and catalyst. [ABSTRACT FROM AUTHOR]

BACKGROUND: The widespread use of chemical herbicides and the growing issue of weed resistance pose significant challenges in agriculture. To address these problems, there is a pressing need to develop biological herbicides based on bacterial metabolites. RESULTS: In this study, we investigated the impact of the cell‐free culture filtrate (CFCF) from the ZT isolate, a bacilliform bacterium obtained from diseased wheat seeds, on the germination and seedling growth of various plant species, including wild oat, ryegrass, redroot, wheat, and chickpea. The results revealed that CFCF had a detrimental effect on the fresh and dry weight of stems and roots in most of the studied plants, except chickpeas. The CFCF was further subjected to separation into aqueous and organic phases using chloroform, followed by the division of the aqueous phase into 13 fractions using an alumina column. Notably, both the aqueous phase (20%) and all 13 fractions (ranging from 50% to 83%) displayed the ability to reduce the root length of ryegrass, a monocotyledonous weed. Liquid chromatography–mass spectrometry (LC–MS) analysis identified that fractions 3 and 7, which were effective against ryegrass but not redroot, contained Cry family proteins, including Cry10 Aa, Cry4 Ba, and Cry4 Aa. Additionally, 16s rRNA gene sequencing revealed that the ZT isolate is closely related (98.27%) to Bacillus wiedmannii. CONCLUSION: Conclusively, metabolites from the ZT bacterium hold promise for monocotyledonous weed‐targeted herbicides, providing a constructive strategy to confront agricultural issues tied to chemical herbicides and weed resistance. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Among the potent anticancer agents, methotrexate (MTX) is very effective against many different types of cancer cells. Implementation of chemotherapy along with radiotherapy, known as synchronous chemoradiotherapy, can augment the treatment efficacy. In the present study self‐assembled MTX conjugated mPEG‐PCL copolymer encapsulating gold nanoparticles (AuNPs) hybrid (mPEG‐PCL‐MTX@Au) was developed as both drug carrier and radiosensitizer. After successful synthesis and characterization of hybrids with different techniques such as hydrogen nuclear magnetic resonance (HNMR), Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible (UV‐Vis), x‐ray diffraction analysis (XRD), dynamic light scattering (DLS), and transmission electron microscopy (TEM). The anticancer activity of designed system was evaluated with a series of biological assays. TEM image indicate that AuNPs encapsulated by self‐assembled mPEG‐PCL‐MTX NPs. It was found that the micelle of that shown in the TEM image contains nine gold nanoparticles (NPs). The hydrodynamic diameter of mPEG‐PCL‐MTX@Au was 81.40 ± 8 nm. As a result, at acidic pH, which simulates tumor tissue, the most drug release is done compared to neutral pH conditions, which are the physiological conditions of the body. About 70% of the drug was released in acidic pH, while in alkaline pH, it was about 35%. The effectiveness of cancer treatment was significantly improved by using developed NPs, which introduces simultaneous chemotherapy and radiotherapy. MTT and apoptosis assays show that the mPEG‐PCL‐MTX@Au as the final formulation improves the radiosensitivity of the 4T1 breast cancer cells and also remarkably kills breast cancer. Furthermore, the result revealed that designed hybrid system extremely able to produce reactive oxygen species (ROS) within cancer cells. These results offer powerful evidence for the potential capability of mPEG‐PCL‐MTX@Au in radiosensitization of malignant tumors and opens up a new avenue of research in this area. [ABSTRACT FROM AUTHOR]

A 25 m long sediment core from hypersaline Urmia Lake (north‐west Iran) was studied for the Late Quaternary depositional history and palaeoclimate variations using the abundance and compositional characteristics of Artemia faecal pellets. Sediment analysis is supported by scanning electron microscopy – energy dispersive X‐ray spectroscopy, organic and inorganic carbon content measurements, and stable isotopes (δ13C and δ18O) from faecal pellet carbonates. The imprecise chronology of the core back to 50 kyr bp is supported by ten radiocarbon ages from faecal pellets and bulk sediments. The palaeoenvironmental record is subdivided into four periods: (i) During much of Marine Isotope Stage 3, a period of lake level lowering is characterized by a decreasing amount of faecal pellets, and an increasing amount of coated grains, sulphate minerals and reworked shell fragments. (ii) During late Marine Isotope Stage 3 and early Marine Isotope Stage 2 a lake level lowstand and a lake floor exposure is interpreted based on the relatively low abundance of pellets, which are multicoloured and appear together with volcanic lithics and rounded sulphate minerals. (iii) During late Marine Isotope Stage 2 the record is devoid of pellets but dominated by large sulphate crystals suggesting a prolonged low lake level. (iv) During Marine Isotope Stage 1 a relative lake level highstand is rapidly established with sediments that are highly abundant in fresh pellets. The modern lake level lowstand is represented by a salt crust. The δ13C and δ18O records measured from faecal pellet carbonates suggest a link with the precipitation versus evaporation balance in the lake over time. From bottom to top the linear trend towards more negative delta values illustrates the increasing amount of precipitation arriving at the lake from the Late Pleistocene to the Holocene. Two prominent isotope minima during the Late Pleistocene and one prominent minimum in the early Holocene mark relative high lake levels, which can also be linked to Lake Van in Turkey. [ABSTRACT FROM AUTHOR]

A highly sensitive microwave sensor for solid material characterization is proposed. A novel design consisting of split ring resonators (SRRs) within a three‐port power divider is developed. The three‐port junction is employed as the host to provide two output channels, and SRRs are loaded through electric coupling to the power divider as the sensing area. The SRRs are located symmetrically so that any change in the electric field of the SRRs is transferred equally to the transmission coefficients of the three‐port junction. The operation of the proposed sensor is based on the downshifting of the transmission zero caused by the loading of samples. The two identical outputs help to control cross‐sensitivity due to external effects. The performance of the fabricated prototype on a 22.2 × 18.8 × 1.6 mm3 printed circuit board is presented. It is shown that a 135 MHz shift per unit relative permittivity change can be achieved. The normalized sensitivity is found to be 3%, which is relatively higher than the sensors reported in the literature. [ABSTRACT FROM AUTHOR]

The aim of this study is to assess whether articular cartilage changes in an equine model of post‐traumatic osteoarthritis (PTOA), induced by surgical creation of standard (blunt) grooves, and very subtle sharp grooves, could be detected with ex vivo T1 relaxation time mapping utilizing three‐dimensional (3D) readout sequence with zero echo time. Grooves were made on the articular surfaces of the middle carpal and radiocarpal joints of nine mature Shetland ponies and osteochondral samples were harvested at 39 weeks after being euthanized under respective ethical permissions. T1 relaxation times of the samples (n = 8 + 8 for experimental and n = 12 for contralateral controls) were measured with a variable flip angle 3D multiband‐sweep imaging with Fourier transform sequence. Equilibrium and instantaneous Young's moduli and proteoglycan (PG) content from OD of Safranin‐O‐stained histological sections were measured and utilized as reference parameters for the T1 relaxation times. T1 relaxation time was significantly (p < 0.05) increased in both groove areas, particularly in the blunt grooves, compared with control samples, with the largest changes observed in the superficial half of the cartilage. T1 relaxation times correlated weakly (Rs ≈ 0.33) with equilibrium modulus and PG content (Rs ≈ 0.21). T1 relaxation time in the superficial articular cartilage is sensitive to changes induced by the blunt grooves but not to the much subtler sharp grooves, at the 39‐week timepoint post‐injury. These findings support that T1 relaxation time has potential in detection of mild PTOA, albeit the most subtle changes could not be detected. [ABSTRACT FROM AUTHOR]

Background and Aims: Spinal canal stenosis is one of the most common vertebral column diseases, which can lead to disability. Developing a registry system can help in research on the prevention and effective treatment of it. This study designs a minimum data set (MDS) as the first step in creating a registry system for spinal canal stenosis. Method: The present research is of applied‐descriptive type, performed in 2022. First, the applicable data elements about the disease were selected from a vast range of English and Farsi references, including peer reviewed articles, academic books, credible websites, and medical records of hospitalized patients. Through the extracted data, the primary MDS plan was designed as a questionnaire. The validity of the questionnaire was conducted via asking the opinion of experts (neurosurgeons, physiotherapists, epidemiologists, and health information management specialists). Also, its reliability was calculated via Cronbach ⍺ coefficient, which was 86%. Finally, the MDS of the spinal canal stenosis national registry system (for Iran) was confirmed through a two stage Delphi technique. Data analysis was applied through descriptive statistics via SPSS21 software. Results: The proposed MDS is offered in two general sets of data: administrative and clinical. For the administrative data set, 40 data elements had been proposed, as five classes. Twenty‐six of them were confirmed. In the clinical section, 95 data elements had been proposed in 14 classes; 94 of which were finally confirmed. Conclusion: Since there is no spinal canal stenosis MDS available, this study can be a turning point in the standardization of the data on this disease. Moreover, these precise, coherent, and standard data elements can be contributed to improving disease management and enhancing the public healthcare quality. Also, the MDS proposed in this study can help researchers and experts, design a spinal canal stenosis registry system in other countries. [ABSTRACT FROM AUTHOR]

The NNW Iranian Plateau and west Alborz within the Arabia‐Eurasia collision zone are characterized by three main tectono‐stratigraphic zones, crosscut by the Qezel‐Owzan River (QOR) Basin. The interplay between present‐day deformation and climate, which control the landscape evolution of the region, is still poorly constrained. We addressed this gap by measuring millennial‐scale erosion rates from 10Be‐concentration in the QOR sands along with topographic/climatic metrics analyses. Results reveal low erosion rates in the Plateau and relatively high in the west Alborz. The regional consistency of topographic parameters with geomorphology suggests that they control sediment fluxes in the Plateau, while the surface uplift, active thrust‐faulting, and shallow crustal seismicity in the west Alborz are the main controlling factors. Climate has a secondary role on erosion rates. Furthermore, we calculated exhumation rates from published thermochronometric AFT/AHe ages to determine their relationship with 10Be short‐term data. Results imply that the exhumation rates increased slightly in the Plateau and west Alborz from ∼26 to ∼10 Ma, simultaneous with hard collision processes between the Arabia‐Eurasia. This trend accelerated from ∼10 to ∼2.8 Ma due to the isolation of the Caspian Sea and extreme base‐level fall. From ∼2.8 to ∼2 Ma, base‐level rise occurred under climate influence, and erosion rates decreased. Millennial‐scale data show the erosion rate decreased from ∼2 Ma to the Present‐day, which is attributed to the change in deformation style and fault kinematics from fold/thrusting to mainly strike‐slip faulting. The significantly lower erosion rates in the Plateau compared to west Alborz suggest a relatively stable plateau surface. Plain Language Summary: The Earth's surface is shaped by interactions between tectonics, climate, and erosion. Tectonic activity with ground motion weakens and fractures bedrock, creating debris and physical erosion, particularly in regions experiencing crustal shortening. There is no doubt that tectonics influence climate primarily by growing high mountain ranges and plateaus. Such high topography may create a barrier to atmospheric circulation and alter precipitation that has significant impact on climatic conditions and erosion. A number of regions around the world have shown the link between tectonic and climatic processes, however, we still lack a comprehensive understanding of ongoing deformation/tectonics and climate on the NNW Iranian Plateau and its margin. We used new 10Be concentration data from river sands and integrate these with topographic‐climatic metrics combined with long‐term data to show how this region has evolved over the past 50 Ma. Our findings suggest that millennial‐scale erosion rates have decreased relative to the long term, mainly due to tectonic impact. A key outcome of this study is that the results indicate that the landscape is in a quasi‐equilibrium in the Iranian Plateau and in a transient state in the west Alborz. Key Points: Acceleration of post‐collisional AFT/AHe derived exhumation rates (∼10 to ∼2.8 Ma) under impact of tectonics and base‐level fluctuationsDeceleration of 10Be millennial‐scale erosion rates compared to the long‐termNearly stable Plateau since Pliocene due to tectonic quiescence, evolving margin due to tectonic/climate coupling in Pliocene‐Quaternary [ABSTRACT FROM AUTHOR]

Background and Aims: Discharge by personal satisfaction is a condition in which the patient leaves the hospital before completing the care period against medical advice. Thus, this study aims to identify and analyze the reasons for discharge with the personal satisfaction of hospitalized patients. Methods: The study was descriptive‐analytical being performed in 2021. The study population was 2869 discharged inpatients with personal satisfaction. Sampling was done by random and census. The data were collected using a checklist and a researcher‐made questionnaire whose validity and reliability were confirmed. The data were analyzed using SPSS24 by K‐Score test for qualitative and variance for quantitative variables. Results: The discharge rate by personal satisfaction was 7.01%, the average age was 42 years, and the average length of stay was 4 days. Further, 57.1% of patients were female, 63.7% were married, and 21% were babies. A total of 22.77% of the patients were discharged with the father's consent, of which 13.2% were re‐admitted. The most common reasons for the discharge were feeling of recovery (47.2%), the hospital being educational (30%), and dissatisfaction with the services of doctors (51.6%). Discharge with personal satisfaction had a significant relationship with the length of hospitalization (p < 0.001) and type of hospital (p = 0.04). Conclusion: The feeling of recovery, the educational nature of the hospital, and dissatisfaction with the services of doctors were the most common reasons for discharge with personal satisfaction. Therefore, monitoring the provision of services, establishing proper patient‐doctor communication, and increasing the awareness of patients and parents could reduce this type of discharge and its consequences. [ABSTRACT FROM AUTHOR]

Background and Aims: Analyzing the utilization of health services is necessary for allocating the resources and planning the provision of health services. The present study aimed at investigating the utilization and cost of outpatient services and the factors affecting it among the insurees of the Iran Health Insurance Organization in Fars province in 2019. Methods: The study population consisted of all Iran Health Insurance Organization insurees in Fars province in 2019 (n = 2,618,973). The data on the utilization and cost of the services were extracted from the information systems of Fars Health Insurance Organization. The descriptive statistics of the utilization and cost of outpatient services were provided by gender, age, and type of insurance fund. The effects of different factors on the utilization and cost of various services were also investigated using univariate analysis as well as cross‐sectional regression. The data analysis was done using EXCEL and STATA 15 software as well. Results: The average utilization rates of laboratory, drug, and radiology services were 0.940, 0.945, and 0.108 prescriptions per year, respectively. In addition, the mean costs of laboratory, drug, and radiology services were $1.13, $7.44, and $2.26 per year, respectively. The univariate and multivariate analyses showed that gender, type of insurance fund, and age had significant effects on the utilization and costs of laboratory, drug, and radiology services (p < 0.05). Conclusion: The utilization and expenditure of outpatient services were higher among the elderly and women. To control the costs of insurance organizations, it is helpful to identify the effective factors. In addition, due to the increasing trend of aging in Iran, it seems necessary to periodically monitor the pattern of the elderly people's utilization of health services and to plan to increase sustainable resources for insurance financing in the coming years. [ABSTRACT FROM AUTHOR]

Summary: Background: Post‐traumatic osteoarthritis is a frequent joint disease in the horse. Currently, equine medicine lacks effective methods to diagnose the severity of chondral defects after an injury. Objectives: To investigate the capability of dual‐contrast‐enhanced computed tomography (dual‐CECT) for detection of chondral lesions and evaluation of the severity of articular cartilage degeneration in the equine carpus ex vivo. Study design: Pre‐clinical experimental study. Methods: In nine Shetland ponies, blunt and sharp grooves were randomly created (in vivo) in the cartilage of radiocarpal and middle carpal joints. The contralateral joint served as control. The ponies were subjected to an 8‐week exercise protocol and euthanised 39 weeks after surgery. CECT scanning (ex vivo) of the joints was performed using a micro‐CT scanner 1 hour after an intra‐articular injection of a dual‐contrast agent. The dual‐contrast agent consisted of ioxaglate (negatively charged, q = −1) and bismuth nanoparticles (BiNPs, q = 0, diameter ≈ 0.2 µm). CECT results were compared to histological cartilage proteoglycan content maps acquired using digital densitometry. Results: BiNPs enabled prolonged visual detection of both groove types as they are too large to diffuse into the cartilage. Furthermore, proportional ioxaglate diffusion inside the tissue allowed differentiation between the lesion and ungrooved articular cartilage (3 mm from the lesion and contralateral joint). The mean ioxaglate partition in the lesion was 19 percentage points higher (P < 0.001) when compared with the contralateral joint. The digital densitometry and the dual‐contrast CECT findings showed good subjective visual agreement. Main limitations: Ex vivo study protocol and a low number of investigated joints. Conclusions: The dual‐CECT methodology, used in this study for the first time to image whole equine joints, is capable of effective lesion detection and simultaneous evaluation of the condition of the articular cartilage. [ABSTRACT FROM AUTHOR]

Ethosuximide (ETX) is a common antiepileptic drug in the first line of absence epilepsy. In this study, for the first time, an economical and efficient electro‐membrane (EME) method for determination of ETX in a complex biological matrix using HPLC‐UV has been developed. Factors affecting conventional EME were evaluated. 1‐Octanol was immobilized in a polypropylene membrane and a voltage of 35 V was applied between two platinum electrodes for 15 min. The pH of acceptor and donor phases for ionization of ETX was adjusted to 13 and 11, respectively. Under optimal microextraction conditions, the enrichment factor was 21.02 and the linear range of ETX was 0.25 to 8.00 μg/mL with an acceptable R2 ≥ 0.9986. Inter‐day and intra‐day precision and accuracy of the suggested method were calculated with RSD < 9.5% and relative error <7.0%, respectively. The mean relative recovery of ETX in the human saliva and plasma samples was 81.68% and 74.47, respectively; while limit of detection and quantification concentrations were 0.08 and 0.25 μg/mL, respectively. Furthermore, to evaluate the application of the method, plasma and saliva samples of volunteers administering a single dose of ETX were analyzed successfully by EME‐HPLC‐UV method. [ABSTRACT FROM AUTHOR]

Arctic warming and permafrost thaw visibly expose changes in the landscape of the Lena River delta, the largest Arctic delta. Determining the past and modern river regime of thick deltaic deposits shaping the Lena River mouth in north‐eastern Siberia is critical for understanding the history of delta formation and carbon sequestration. Using a 65 m long sediment core from the delta apex a set of sedimentological techniques is applied to aid in reconstructing the Lena River history. The analysis includes: (i) grain‐size measurements and the determination of the bedload composition; (ii) X‐ray fluorescence, X‐ray diffractometry, and magnetic susceptibility measurements and heavy mineral analysis for tracking mineral change; (iii) pH, electrical conductivity, ionic concentrations, and the δ18O and δD stable isotope composition from ground ice for reconstructing permafrost formation. In addition; (iv) total and dissolved organic carbon is assessed. Chronology is based on; (vi) radiocarbon dating of organic material (accelerator mass spectrometry and conventional) and is complemented by two infrared – optically stimulated luminescence dates. The record stretches back approximately to Marine Isotope Stage 7. It holds periods from traction, over saltation, to suspension load sedimentation. Minerogenic signals do not indicate provenance change over time. They rather reflect the change from high energy to a lower energy regime after Last Glacial Maximum time parallel to the fining‐up grain‐size trend. A prominent minimum in the ground ice stable isotope record at early Holocene highlights that a river arm migration and an associated refreeze of the underlying river talik has altered the isotopic composition at that time. Fluvial re‐routing might be explained by internal dynamics in the Lena River lowland or due to a tectonic movement, since the study area is placed in a zone of seismic activity. At the southern Laptev Sea margin, onshore continental compressional patterns are bordering offshore extensional normal faults. [ABSTRACT FROM AUTHOR]

The development of selective and simple methods for the determination of different analytes is of great interest. This is the first time to show the applicability of graphene oxide‐chitosan (GO‐CS) nanocomposite for designing an electrochemical nanosensor for determination of Amlodipine, Valsartan, and Hydrochlorothiazide, simultaneously. Differential pulse voltammetrics current of AML, HCT, and VAL increased linearly in the ranges of 0.1–110, 0.1–110, and 1–230 μM with LOD of 5.5×10−2, 3.5×10−2 and 8.6×10−2 μM, respectively. Finally, GO‐CS/GCE was used for the detection of these drugs in commercial tablets and compared with the reference method (HPLC). [ABSTRACT FROM AUTHOR]

A new series of spiro[indene‐1,2′‐quinazolin]‐4′(3′H)‐one derivatives 4a–m were synthesized via a one‐pot method and evaluated for anticonvulsant activities using pentylenetetrazole (PTZ) and maximal electroshock (MES)‐induced seizures. Obtained results demonstrated that these compounds have not anticonvulsant activity in PTZ test while are active in the MES test. Among the synthesized compounds, the best anticonvulsant activity was obtained with compound 4h. This compound also was not neurotoxic. Given that the title new compounds have the pharmacophore requirement for benzodiazepine (BZD) receptor agonist, the most potent compound was assayed in vivo and in silico as BZD receptor agonist. After treatment with flumazenil as a standard BZD receptor antagonist, anticonvulsant activity of compound 4h decreased. Therefore, the involvement of BZD receptors in anticonvulsant activity of this compound confirmed. Furthermore, docking study of compound 4h in the BZD‐binding site of GABAA receptor confirmed that this compound interacted with the important residues. [ABSTRACT FROM AUTHOR]

Background and Aims: One of the main vital needs for self‐care in patients with advanced cancer is information need. Meeting this need has significant positive effects on improving their treatment and care. This study was conducted to identify the unmet information needs of patients with advanced cancer in Iran. Methods: This exploratory study was performed from July to February 2021 in the Kerman University of Medical Sciences cancer treatment centers. Oncologists selected eligible patients by purposeful sampling method. Semistructured and in‐depth interviews were conducted with selected patients to collect data. Interviews continued until data saturation. Each interview was audio‐recorded and transcribed verbatim. Results: In the interviews, 15 patients with advanced cancer ranging in age from 43 to 65 years participated. The most common type of cancer in women was breast (71.4%) and prostate (50%) in men. The two main categories of "types of unmet information needs" and "reasons for not meeting information needs" were extracted from the analysis of patient interviews, with six and four subcategories, respectively. Conclusion: Cancer patients had a large number of unmet information needs. At the time of identifying the unmet information needs of cancer patients, the basic reasons for not meeting these needs should also be considered because cultural differences and social gaps in societies are inevitable. [ABSTRACT FROM AUTHOR]

Nearly half a century ago, two papers postulated the likelihood of lunar lava tube caves using mathematical models. Today, armed with an array of orbiting and fly‐by satellites and survey instrumentation, we have now acquired cave data across our solar system—including the identification of potential cave entrances on the Moon, Mars, and at least nine other planetary bodies. These discoveries gave rise to the study of planetary caves. To help advance this field, we leveraged the expertise of an interdisciplinary group to identify a strategy to explore caves beyond Earth. Focusing primarily on astrobiology, the cave environment, geology, robotics, instrumentation, and human exploration, our goal was to produce a framework to guide this subdiscipline through at least the next decade. To do this, we first assembled a list of 198 science and engineering questions. Then, through a series of social surveys, 114 scientists and engineers winnowed down the list to the top 53 highest priority questions. This exercise resulted in identifying emerging and crucial research areas that require robust development to ultimately support a robotic mission to a planetary cave—principally the Moon and/or Mars. With the necessary financial investment and institutional support, the research and technological development required to achieve these necessary advancements over the next decade are attainable. Subsequently, we will be positioned to robotically examine lunar caves and search for evidence of life within Martian caves; in turn, this will set the stage for human exploration and potential habitation of both the lunar and Martian subsurface. Plain Language Summary: We have now acquired cave data across our solar system—including the identification of potential cave entrances on the Moon, Mars, and at least nine other planetary bodies. These discoveries gave rise to the study of planetary caves. To help advance this field, we conducted an expert‐opinion based social survey to identify a strategy to explore caves beyond Earth. We focused primarily on astrobiology, the cave environment, geology, robotics, instrumentation, and human exploration. First, we assembled a list of 198 science and engineering questions. Then, through a series of social surveys, 114 scientists and engineers winnowed down the list to the top 53 highest priority questions. This exercise resulted in identifying emerging and crucial research areas that require robust development to ultimately support a robotic mission to a planetary cave—principally the Moon and/or Mars. With the necessary financial investment and institutional support, the research and technological development required to achieve these necessary advancements over the next decade are attainable. Subsequently, we will be positioned to robotically examine lunar caves and search for evidence of life within Martian caves; in turn, this will set the stage for human exploration and potential habitation of both the lunar and Martian subsurface. Key Points: Robotics and instrument advancements identified as linchpin focal areas for in situ study of planetary cavesResearch and technological development required for lunar and/or Martian cave exploration is achievable in next decade with proper investmentFirst application of systematic and statistically rigorous social survey to identify science and engineering requirements in planetary science [ABSTRACT FROM AUTHOR]

Background: Bovine immunodeficiency virus (BIV) is a member of the Retroviridae family causing a progressive lifelong infection in cattle and buffaloes. Objective: Despite the worldwide distribution of the virus, the studies concerning the prevalence of BIV in buffalo populations have not been conducted in Iran as yet. Methods: The BIV proviral DNA was surveyed in 120 whole blood samples of water buffaloes in southwestern Iran. Nested PCR was employed to amplify a 298‐bp fragment of the pol gene. The BIV Pol sequence was detected in 9.1% of the samples. Among PCR‐positive samples, two amplified fragments were confirmed by nucleotide sequencing. Results and conclusions: The studied sequences were completely identical to each other and had more than 98%–99% nucleotide homology to R‐29 and HXB3 sequences previously deposited in GenBank. Some point mutations that caused coding substitutions were observed in the studied isolates, compared to other strains. A phylogenetic tree was generated based on the BIV Pol nucleotide sequences reported from other countries. All the BIV strains originated from a unique main cluster and then separated from each other over time. This is the first report on the molecular detection of BIV infections in water buffalo populations in Iran. The wide distribution of BIV in different countries including Iran indicates the importance of the infection as it relates to animal health. Although buffaloes show greater resistance to diseases, they should be considered a health risk to cattle. Furthermore, BIV has negative effects on buffalo milk production and can predispose them to secondary infections. Hence, the findings of this study can advance our understanding of the occurrence of BIV infection in Iran, which can play an important role in the distribution of the disease worldwide. [ABSTRACT FROM AUTHOR]

This study is focused on proposing a new design and setup for electromembrane extraction. A new cap was designed and conductive vials of different shapes were fabricated using three‐dimensional printing. The new cap holds three fibers to enhance electromembrane extraction recovery. Conductive vials can simultaneously perform as electrodes therefore, there is no need to include an electrode in sample solutions. Phenobarbital and phenytoin were used as model compounds to assess the setup performance. Under optimal conditions, these analytes were extracted from the sample solution at pH = 9 to the acceptor solution at pH = 13 with a voltage of 40 V for 20 min, while 1‐octanol was employed as the supported‐liquid‐membrane. The influence of conductive vials geometry on the recovery was examined and the effects of different shapes were studied by performing numerical simulation to establish electric potential distribution. Of the vials tested with circular, triangular, and floral‐like cross‐sections the latter exhibited the best voltage distribution. The circular vial had the highest recovery attributed to its better hydrodynamic shape, which allows rapid fluid sample transport and therefore enhanced system recovery. The extraction recovery and relative standard deviation of the circular vial with three fibers were 33.0 and 7.6 for phenobarbital and 42.2 and 10.4 for phenytoin. [ABSTRACT FROM AUTHOR]

Cancer immunotherapy has made improvements due to the advances in chimaeric antigen receptor (CAR) T cell development, offering a promising treatment option for patients who have failed to respond to traditional treatments. In light of the successful use of adoptive CAR T cell therapy for cancer, researchers have been inspired to develop CARs for the treatment of other diseases beyond cancers such as viral infectious diseases. Nonetheless, various obstacles limit the efficacy of CAR T cell therapies and prevent their widespread usage. Severe toxicities, poor in vivo persistence, antigen escape, and heterogeneity, as well as off‐target effect, are key challenges that must all be addressed to broaden the application of CAR T cells to a wider spectrum of diseases. The key advances in CAR T cell treatment for cancer and viral infections are reviewed in this article. We will also discuss revolutionary CAR T cell products developed to improve and enhance the therapeutic advantages of these treatments. [ABSTRACT FROM AUTHOR]

This study investigates the differing role of enforcement on the formation of venture capital (VC) syndication networks. We conjecture that public enforcement, with strong investigative powers against any syndicate member, discourages the formation of denser syndication networks due to misconduct risk by a member. By contrast, private enforcement, with strong disclosure and liability standards, enables denser syndication networks, through clear liability rules, standardized securities contracts and cost‐sharing amongst syndicate members. Our VC data from 31 countries show a negative impact of public enforcement on VC networks and partially support the positive impact of private enforcement depending on cultural conditions. [ABSTRACT FROM AUTHOR]

Background: Skin wound healing has always been a challenging subject as it involves the coordinated functioning of various cells and molecules. Any disorder in wound healing can cause healing failure and result in chronic wounds. In this study, we hypothesized that co‐cultured dermal fibroblasts (DFs) and Wharton's jelly mesenchymal stem cells (WJ‐MSCs) seeded on an acellular amniotic membrane scaffold could be used to promote skin regeneration in chronic ulcers. Materials and Methods: In this case series, the chronic wounds of five diabetic patients aged between 30 and 60 years were treated with co‐cultured WJ‐MSCs and DFs seeded on an acellular amniotic membrane. Treatment was applied and the wound healing process was evaluated every three days for nine days, with the patients being subsequently followed up for one month. The wound healing percentage, time taken for the wound to heal, and wound size were monitored. Results: The mean wound healing rate (WHR) increased progressively in all lesions. The mean percentage of wound healing after transplantation of the biological scaffold enriched with WJ‐MSCs and autologous DFs after treatment was 93.92%, respectively. The healing percentage significantly increased after three days; significant decreases in wound size and healing time were recorded after six and nine days of treatment, respectively (p < 0.002); and total skin regeneration and re‐epithelialization were achieved by the ninth day of treatment. There were no side effects or complications. Conclusion: Given the current problems and complications presented by chronic wounds, Novel Clinical approaches involving cell therapy and tissue engineering can be regarded as an attractive therapeutic option for the treatment of chronic and difficult‐to‐heal wounds. [ABSTRACT FROM AUTHOR]

This study aimed to quantify the long‐term progression of blunt and sharp cartilage defects and their effect on joint homeostasis and function of the equine carpus. In nine adult Shetland ponies, the cartilage in the radiocarpal and middle carpal joint of one front limb was grooved (blunt or sharp randomized). The ponies were subjected to an 8‐week exercise protocol and euthanized at 39 weeks. Structural and compositional alterations in joint tissues were evaluated in vivo using serial radiographs, synovial biopsies, and synovial fluid samples. Joint function was monitored by quantitative gait analysis. Macroscopic, microscopic, and biomechanical evaluation of the cartilage and assessment of subchondral bone parameters were performed ex vivo. Grooved cartilage showed higher OARSI microscopy scores than the contra‐lateral sham‐operated controls (p < 0.0001). Blunt‐grooved cartilage scored higher than sharp‐grooved cartilage (p = 0.007) and fixed charge density around these grooves was lower (p = 0.006). Equilibrium and instantaneous moduli trended lower in grooved cartilage than their controls (significant for radiocarpal joints). Changes in other tissues included a threefold to sevenfold change in interleukin‐6 expression in synovium from grooved joints at week 23 (p = 0.042) and an increased CPII/C2C ratio in synovial fluid extracted from blunt‐grooved joints at week 35 (p = 0.010). Gait analysis outcome revealed mild, gradually increasing lameness. In conclusion, blunt and, to a lesser extent, sharp grooves in combination with a period of moderate exercise, lead to mild degeneration in equine carpal cartilage over a 9‐month period, but the effect on overall joint health remains limited. [ABSTRACT FROM AUTHOR]

The current article is focused on introduction and validation of electro‐membrane extraction along with a high‐performance liquid chromatography combined with UV detector for the identification of the antiepileptic drugs phenytoin (PT) and phenobarbital (PB) in plasma, saliva, and urine. In addition, 3D‐printing was utilized to fabricate the designed caps required for fixing the electrodes in the prepared setup, giving us a high potential in device fabrication with various dimensions. To arrive at the highest extraction of PB and PT, the parameters impacting the extraction efficiency of electromembrane extraction for example, supported liquid membrane composition, sample solution pH, applied voltage, extraction time along with effect of graphene oxide are examined in detail. In optimized experimental conditions, the calibration plots were obtained to be linear on the concentration range of 25–500 ng/L with the detection limit and quantitation limit of 7.5 and 25 ng/L for both of PB and PT. Relative standard deviations of <9.9% and <9.1% were found for PB and PT, respectively and the enrichment factor for the presented method was computed to be 25.4 and 44.7 for PB and PT, respectively. The proposed method was satisfactorily employed in determination of PB and PT in plasma, urine and saliva. [ABSTRACT FROM AUTHOR]

The configuration and timing of the Arabia‐Eurasia continental collision, part of the broader Alpine‐Himalayan collisional system, remains controversial. We conducted sandstone petrology, detrital zircon U‐Pb‐Hf isotopic and trace element analysis, and Cr‐spinel electron microprobe geochemical analysis of samples from Paleocene to Miocene peripheral foreland strata in interior Fars, southern Iran. These data were used to test competing models for ophiolite obduction and Arabia‐Eurasia collision. In addition, we applied these data to compare the history of outward and upward growth of the Zagros and Himalayan‐Tibetan segments of the Alpine‐Himalayan collisional orogenic belt. The first appearance of radiolarian‐rich chert conglomerate, 100–90 Ma detrital zircons with positive ɛHf(t) values from +1 to +20 and midocean ridge geochemical affinity, and suprasubduction zone (SSZ) affinity Cr‐spinel is in the lower and middle Sachun Formation. These data indicate that obduction occurred before deposition of the upper Maastrichtian‐lower Paleocene Sachun Formation and developed in an intra‐oceanic setting rather than an Arabia‐Eurasia collision setting. Abundant continental‐arc affinity detrital zircon with 180–160 Ma and 50–27 Ma age‐probability peaks and varied ɛHf(t) values are present in the upper Oligocene‐lower Miocene Razak and Agha Jari formations, indicating sedimentary overlap with Eurasia. SSZ‐affinity Cr‐spinel in all samples indicates that ophiolitic rocks were a continual source of detritus in the foreland basin since Paleocene. The depositional age of the basal Razak Formation is between 25.7 and 21.5 Ma. Therefore, we interpret that collision between Arabia and Eurasia must have been initiated before deposition of the Razak Formation. Key Points: Neyriz ophiolite was obducted onto Arabia during Late Cretaceous‐early Paleocene timeArabia‐Eurasia intercontinental collision occurred before deposition of 25.7–21.5 Ma Razak formationDecreases in propagation rate of foreland basin system play an important role in the growth of high elevation orogenic plateau [ABSTRACT FROM AUTHOR]

When operating in confined spaces or near obstacles, collision‐free path planning is an essential requirement for autonomous exploration in unknown environments. This study presents an autonomous exploration technique using a carefully designed collision‐free local planner. Using LiDAR range measurements, a local end‐point selection method is designed, and the path is generated from the current position to the selected end‐point. The generated path showed the consistent collision‐free path in real‐time by adopting the Euclidean signed distance field‐based grid‐search method. The results consistently demonstrated the safety and reliability of the proposed path‐planning method. Real‐world experiments are conducted in three different mines, demonstrating successful autonomous exploration flights in environment with various structural conditions. The results showed the high capability of the proposed flight autonomy framework for lightweight aerial robot systems. In addition, our drone performed an autonomous mission in the tunnel circuit competition (Phase 1) of the DARPA Subterranean Challenge. [ABSTRACT FROM AUTHOR]

More attention of the medical staff to this sign can lead to faster diagnosis and timely treatment of patients. [ABSTRACT FROM AUTHOR]