1. Comparison of peripheral blood automated hematopoietic progenitor cell count and flow cytometric CD34+ cell count.
- Author
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Hagenkötter, Mischa, Mika, Thomas, Ladigan‐Badura, Swetlana, Schork, Karin, Eisenacher, Martin, Schroers, Roland, and Baraniskin, Alexander
- Abstract
Background: Stem cell apheresis in the context of autologous stem cell transplantation requires an accurate cluster of differentiantion 34 (CD34+) count determined by flow cytometry as the current gold standard. Since flow cytometry is a personnel and time‐intensive diagnostic tool, automated stem cell enumeration may provide a promising alternative. Hence, this study aimed to compare automated hematopoietic progenitor enumeration carried out on a Sysmex XN‐20 module compared with conventional flow cytometric measurements. Methods: One hundred forty‐three blood samples from 41 patients were included in this study. Correlation between the two methods was calculated over all samples, depending on leukocyte count and diagnosis. Results: Overall, we found a high degree of correlation (r = 0.884). Furthermore, correlation was not impaired by elevated leukocyte counts (>10 000/μL, r = 0.860 vs <10 000/μL, r = 0.849; >20 000/μL, r = 0.843 vs <20 000/μL, r = 0.875). However, correlation was significantly impaired in patients with multiple myeloma (multiple myeloma r = 0.840 vs nonmyeloma r = 0.934). Summary: Stem cell measurement carried out on the Sysmex XN‐20 module provides a significant correlation with flow cytometry and might be implemented in clinical practice. In clinical decision‐making, there was discrepancy of under 15% of cases. In multiple myeloma patients, XN‐20 should be used with caution. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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