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Cultivation of Miscanthus x giganteus L. (Mis) with annual harvest of biomass could provide an additional C source for farmers. To test the potential of Mis-C for immobilizing inorganic N from slurry or manure and as a C source for soil organic matter build-up in comparison to wheat (Triticum aestivum L.) straw (WS), a greenhouse experiment was performed. Pot experiments with ryegrass (Lolium perenne L.) were set up to investigate the N dynamics of two organic fertilisers based on Mis at Campus Klein-Altendorf, Germany. The two fertilisers, a mixture of cattle slurry and Mis as well as cattle manure from Mis-bedding material resulted in a slightly higher N immobilisation. Especially at the 1st and 2nd harvest, they were partly significantly different compared with the WS treatments. The fertilisers based on Mis resulted in a slightly higher microbial biomass C and microbial biomass N and thus can be identified as an additional C source to prevent nitrogen losses and for the build-up of soil organic matter (SOM) in the long-term. [ABSTRACT FROM AUTHOR]