1. A therapeutic agent of ursolic acid demonstrates potential application in aquaculture.
- Author
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Li, Bo-Yang, Qin, Jia-Cheng, Shen, Yu-Feng, Yang, Fei, Wang, Tao, Ling, Fei, and Wang, Gao-Xue
- Subjects
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URSOLIC acid , *LARGEMOUTH bass , *CTENOPHARYNGODON idella , *AQUACULTURE , *VIRAL load - Abstract
• UA effectively inhibits MSRV proliferation in vitro. • UA has a protective effect on MSRV-infected fish. • UA is a valuable agent for MSRV in the industry. Micropterus salmoides rhabdovirus (MSRV) has a high mortality rate and causes huge economic losses to the aquaculture industry. In this study, we identified that ursolic acid (UA) had antiviral efficacy against MSRV in vitro and in vivo. The results showed that UA inhibited MSRV replication in grass carp ovary (GCO) cells with a half-maximal inhibitory concentration (IC 50) of 5.55 μM, reduced viral titers and decreased cytopathic effects (CPE). Mechanistically, UA does not directly damage viral particles. On the other hand, UA inhibits MSRV replication by altering viral binding and release. Furthermore, pre- and post-treatment assays revealed that UA had preventive and therapeutic effects. For in vivo studies, UA could enhance the survival rate of MSRV-infected largemouth bass. Similarly, UA reduced the viral load of MSRV in the heart, spleen and brain at 3, 5 and 7 d post-infection. In conclusion, UA is an effective inhibitor of rhabdovirus in aquaculture. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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