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Due to the fragility of the habitats in mountain nature reserves, the vegetation is extremely sensitive to climate change, and its phenological changes are more specific. Therefore, it is of great significance to study the effects of topography and climate on the vegetation phenology in mountain nature reserves. Based on the vegetation phenology data retrieved from MODIS EVI2 during 2000 to 2017, combined with temperature data, spatial trend analysis and correlation analysis methods were used to study the effects of topographic and climatic factors on vegetation phenology in the Niubeiliang Nature Reserve of the Qinling Mountains. The results showed that the GSS (growing season start) was advanced with a rate of 4.24 days/10a, and the rates in the northern and southern slopes were almost the same; the GSE (growing season end) was delayed with a rate of 3.29 days/10a, and the rate in the northern slope was faster; and the GSL (growing season length) was prolonged. There were seasonal differences and north–south differences in the effects of topography on the phenophase. The phenophase changed regularly with the increase in altitude. The higher the altitude, the more significant the effect. The steeper the slope, the later the GSS, the earlier the GSE, and the more significant its effect on the GSE. The aspect had little effect on GSS but a more significant effect on GSE, which was the latest on the sunny slope and the earliest on the zero slope. Temperature affected both the GSS advance and the GSE delay, and both had a time-lag effect of approximately 2–3 months. Its effect was more significant in the GSE, in the southern slopes, and in the high-altitude areas. [ABSTRACT FROM AUTHOR]