1. Inactivation effects of plasma-activated water on Fusarium graminearum.
- Author
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Guo, Jian, Wang, Jiaoyu, Xie, Hui, Jiang, Junlong, Li, Chunyuan, Li, Wanting, Li, Ling, Liu, Xingquan, and Lin, Fucheng
- Subjects
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MEMBRANE permeability (Biology) , *FUSARIUM , *COMMERCIAL products , *GENE expression - Abstract
The continuous usage of fungicides poses a potential threat to the environment, ranging from mere irritation to being very toxic to human beings and organisms. Plasma-activated water (PAW) has recently gained much interest as a promising candidate to inactivate phytopathogens. However, the inactivation mechanisms of PAW are still not well understood. In this study, the effect of PAW on the cellular responses of Fusarium graminearum and the efficacy of PAW against F. graminearum in vivo and in vitro were investigated. The results showed that microbial activity of spores was significantly inhibited by PAW treatment (P < 0.05). The symptoms caused by F. graminearum were significantly reduced on the wheat spikelets. PAW could induce cell wall sculpturing, membrane permeability changes, and mitochondrial dysfunction. Differential gene expression analysis also confirmed that the cell membrane, the cell wall and the mitochondria were the organelles most affected by PAW. The results from this study facilitate the understanding of the mechanisms underlying the responses of F. graminearum to PAW and indicate the potential of PAW as a fungicidal agent or an effective supplement to fungicides. [Display omitted] • The viability of F. graminearum was notably inhibited by PAW. • The symptoms caused by F. graminearum were significantly reduced on the spikelets. • Oxidative stress induced cell wall sculpturing, membrane permeability change. • PAW caused the mitochondrial dysfunction. • Cell wall, membrane and mitochondria were the most affected organelles by PAW. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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