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Ecosystems in arid regions with sandy substrates are vulnerable to various environmental challenges. Understanding the vegetation evolution of such regions over a long timescale and the possible drivers of change is important for optimizing ecological restoration. In this paper, we present multi-proxy data to understand Holocene vegetation dynamics and their drivers in the Horqin Sandy Land in northern China. Phytoliths are used to reconstruct vegetation; grain size and magnetic susceptibility are used to reconstruct the climate; and sedimentary charcoal contents and the numbers of regional archaeological sites are used to reconstruct the fire activity and human activity intensity, respectively. Results indicate that the Horqin Sandy Land was covered mainly by grassland vegetation during the Holocene, and that the trajectory of vegetation evolution was as follows: sparse and species-poor Leymus chinensis grassland from 9300 to 6150 cal yr BP, relatively sparse and species-rich mesophytic herb grassland from 6150 to 2300 cal yr BP, and dense and species-rich L. chinensis grassland from 2300 to 250 cal yr BP. Climate change was the main driver of vegetation evolution, but fire and human activities also influenced the vegetation to varying degrees. Fire made the greatest contribution to the vegetation change during the 6150–2300 cal yr BP interval, and human activities contributed throughout the 8200–2300 cal yr BP interval. The evolution of plant species richness and the number of C 3 plants show a significant ∼1000-yr periodicity during 9300–4500 cal yr BP, which we speculate was caused by the effect of solar activity on East Asian monsoon intensity, which likely controlled the vegetation change on a millennial timescale. • The Holocene vegetation in Horqin Sandy Land is qualitatively reconstructed. • Climate dominated the vegetation evolution, while fire and human activity were additional influences. • The vegetation change shows a ∼ 1000-yr periodicity during 9300–4500 cal yr BP. • The vegetation change on a millennium cycle was likely driven by the solar activity. [ABSTRACT FROM AUTHOR]