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In this article, an Au/Ti 3 C 2 T x MXene hybrid layered D-shaped Photonic Crystal Fiber (DPCF) based Surface Plasmonic Resonance (SPR) biosensor is proposed. The D-shape of PCF is used to make the sensor practically more feasible. The MXene-Au hybrid thin films are placed over the DPCF to induce the surface plasmon polariton (SPP). The resonance wavelength shift occurs when analytes refractive index (RI) is changed. The numerical analysis has been performed by finite element analysis. The results have shown the wavelength sensitivity of 7000 nm/RIU and 13,000 nm/RIU for MXene layer thickness of 14 nm and 27 nm respectively. On comparing, it has been observed that the MXene/Au layered sensor has achieved 3.5 to 6.5 times higher wavelength-sensitivity compared to without MXene thin film. In addition, sensing parameters like resolution, amplitude sensitivity and figure of merit has also been investigated. The proposed sensor is used for sensing the analytes having RI range of 1.33–1.39. The range of RI covers most of the blood cells compositions, cancerous cells, protein and viruses. Therefore, this study can facilitate the use of MXene material with D-shaped PCF to detect the biochemical and chemical liquids. Photonic crystal fiber, MXene material, Gold, Surface plasmon resonance, Biomolecules. • A surface plasmon resonance sensor based on D-shape of PCF is proposed using Mxene/Au material thin film for the first time. • The sensing analytes having a refractive index (RI) range of 1.33–1.39 are used. The range of RI covers most of the bio analyte's RI like blood cells, cancerous cells, RNA/DNA, malaria-infected RBCs, protein and viruses. • The results show the wavelength sensitivity of 7000 nm/RIU and 13,000 nm/RIU for Mxene layer thickness 14 nm and 27 nm respectively. • On comparing, it has been observed that the Mxene/Au layered sensor has 3.5 to 6.5 times higher wavelength-sensitivity than without the Mxene thin layer. • The effect of the Mxene layer on other sensing parameters like resolution, amplitude sensitivity and figure of merit has also been investigated. [ABSTRACT FROM AUTHOR]