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C17H17N5, monoclinic, P21/c (no. 14), a = 9.4347(9) Å, b = 15.6392(15) Å, c = 11.0939(11) Å, β = 100.215 (2) ° , V = 1611.0(3) Å3, Z = 4, Rgt(F) = 0.0392, wRref(F2) = 0.1105, T = 296 K. [ABSTRACT FROM AUTHOR]

C17H17N5, monoclinic, P21/c (no. 14), a = 9.4347(9) Å, b = 15.6392(15) Å, c = 11.0939(11) Å, β = 100.215 (2) ° , V = 1611.0(3) Å3, Z = 4, Rgt(F) = 0.0392, wRref(F2) = 0.1105, T = 296 K. [ABSTRACT FROM AUTHOR]

[Display omitted] • A simple developed fluorescent probe was synthesized for rapid detection of CN– in colorimetric and fluorometric signal channels. • The crystal structure of the probe is reported. • The probe works in DMSO/HEPES (3:7, v/v) solution with large fluorescent emission (595 nm) and large Stokes shifts (145 nm). • The probe has been successfully applied to the imaging of CN− in C6 cells. In this work, a novel probe R was synthesized via Knoevenagel reaction between 3 H -benzo[ f ]chromium-2-formaldehyde and ethyl cyanoacetate for selective detection of CN– in both colorimetric and fluorescent signal channels. The recognition of CN– was through the nucleophilic reaction of CN– to C = C of probe R , which destroys π-conjugation and blocks the ICT effect of the probe, resulting in colorimetric and fluorometric responses. Probe R showed great sensitivity toward CN–, with large fluorescent emission (595 nm) and low detection limit (0.70 μM). Moreover, probe R can detect exogenous CN– in living cells. [ABSTRACT FROM AUTHOR]

• A pyrazine-containing hydrazone sensor for Al3+ has been developed. • The crystal structure of 1, 1-Cu and 1-Ni have been reported. • The reluctant Al3+ complex of the sensor could recognize F− among various anions. • The sensor could be utilized to detect exogenous Al3+ and F− in HeLa cells. The hydrazone sensor (1) derived from pyrazine-2-carbohydrazide and 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (PMBP) was applied to the fluorescent "turn-on" detection of Al3+ in semi-aqueous solutions. The strongly emissive 1 +Al3+ complex could identify F− among other tested anions. The detection limits of sensor 1 for Al3+ and F− were 0.18 and 0.20 μM, respectively, which are both much lower than the corresponding values recommended by the World Health Organization, namely, 7.41 and 79 μM. Several spectral methods, including single crystal X-ray analysis for the crystal structures of the Cu2+ and Ni2+ complexes of sensor 1 , as well as the density-functional theory method, were used to elucidate the binding mode between the sensor and Al3+. 1 can also be used for a selective sensor to continuously monitor Al3+ and F− in living cells. [Display omitted] [ABSTRACT FROM AUTHOR]

Carboxylesterases (CEs) are widely distributed in the body and catalyze the hydrolysis of endogenous and exogenous substrates. They play important roles in drug metabolism, and abnormal levels often result in numerous diseases such as cancers. Therefore, it is important to establish a highly selective and sensitive probe for the real-time sensing of CEs activity. Herein, we synthesized a novel fluorescence-electrochemical multichannel probe 1 containing a ferrocene unit, which not only serves as part of the structure of probe 1 but also provides electrochemical signals. The probe exhibited high selectivity to CEs with a "Turn-On" fluorescence response and was applied for imaging in complicated biological samples including Staphylococcus aureus , living cells, and zebrafish. Furthermore, the hydrolysis of the acetoxy group of probe 1 by CEs decreased the electrochemical signal. All these results suggest that probe 1 can act as a multi-modal probe for real-time tracking of the activity CEs in complex biological environments. • A ferrocene-chalcone-based probe for detecting CEs by fluorescence and electrochemical signals was developed. • Probe 1 exhibited rapid response, good optical performance, high selectivity, and high sensitivity in solution. • The crystal structure of probe 1 and product 1a were determined. • Endogenous imaging-based CEs-detection in a range of S. aureus species, C6 cells, and zebrafish was achieved. [ABSTRACT FROM AUTHOR]