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Research background. Kombucha is a fermented beverage with several health benefits; however, to improve its antioxidant activity, new raw materials such as hop, madimak and hawthorn were included in the present study. Experimental approach. The somatic mutation and recombination test (SMART) was performed on the fruit fly (Drosophila melanogaster) to evaluate the antigenotoxic potential of black tea-flavoured kombucha and three other flavours of kombuchas (hop, madimak and hawthorn) against H2O2- and K2Cr2O7-induced genotoxicity. Furthermore, a lifespan assay was performed to assess the effects of kombuchas on the longevity of the fruit fly. Results and conclusions. According to the results obtained from the SMART assay, hop-flavoured kombucha attenuated genotoxicity induced by H2O2, and madimak-flavoured kombucha reduced genotoxicity induced by H2O2 and K2Cr2O7. Black tea- and hop-flavoured kombucha prolonged the lifespan of the fruit fly (Drosophila melanogaster) after the treatment with  H2O2 and K2Cr2O7. Novelty and scientific contribution. Hop-flavoured kombucha is a promising antioxidant that protects the genome and extends the lifespan of the fruit fly. This study sheds light on novel beverages that can combat ageing and protect against genotoxicity. [ABSTRACT FROM AUTHOR]

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Simple Summary: We aim to determine whether chlorophyll a and chlorophyll b pigments in green vegetables and fruits have antigenotoxic effects against the MeIQx compound, which is frequently formed during the cooking of meat and meat products. In this study, the protective effects of chlorophyll a and chlorophyll b (0.5 and 1 µM) against the heterocyclic amine compound 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx, 4.69 µM, 9.38 µM, 23.45 µM) with somatic mutation and recombination test in Drosophila melanogaster are investigated. Chronic applications are performed to transheterozygous larvae with respect to two recessive genes, mwh (multiple wing hair) and flr3 (flare), by using Drosophila strains. The genotoxic effects of MeIQx are primarily determined for third instars larvae. In antigenotoxicity studies, two different application groups are constituted. While for the first group doses of chlorophyll a, b, and MeIQx are given to the third instars larvae simultaneously, for the second group doses of MeIQx are applied at the third instars after doses of chlorophyll a and b are given to at the second instars larvae. Chlorophyll a and b are effective in reducing genotoxic effects of MeIQx by both applications on individuals and it is observed that the pretreatment method is much more effective than the simultaneous one. There are similar results for chlorophyll a and b in efficacy. [ABSTRACT FROM AUTHOR]