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Start Over Topic heart failure Publication Year Range Last 3 years
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1. Paper electrochemical immunosensor for the rapid screening of Galectin-3 patients with heart failure.

Author

Nihal S, Sarfo D, Zhang X, Tesfamichael T, Karunathilaka N, Punyadeera C, and Izake EL

Subjects
Humans, Immunoassay methods, Saliva chemistry, Biomarkers analysis, Gold chemistry, Galectins analysis, Limit of Detection, Heart Failure diagnosis, Electrochemical Techniques, Paper, Biosensing Techniques methods, Galectin 3 analysis, Blood Proteins
Abstract

A paper electrochemical immunosensor for the combined binding and quantification of the heart failure (HF) biomarker Galectin-3 has been developed. The simple design of the new sensor is comprised of paper material that is decorated with gold nanostructures, to maximize its electroactive surface area, and functionalized with target-specific recognition molecules to selectively bind the protein from aqueous solutions. The binding of the protein caused the blockage of the electron flow to the sensor electroactive surface, thus causing its oxidation potential to shift and the corresponding current to reduce quantitatively with the increase in the protein concentration within the working range of 0.5ng/mL-8ng/mL (LOQ-0.5 ng/mL). This novel sensor was able to quantify Galectin-3 concentration in saliva samples from HF patients and healthy controls within 20 min with good reproducibility (RSD = 3.64%), without the need for complex sample processing steps. The electrochemical measurements of the patient samples were cross validated by ELISA where the percent agreement between the two methods was found to be 92.7% (RSD = 7.20%). Therefore, the new paper immunosensor sensor has a strong potential for rapid and cost-effective screening of the Galectin 3 biomarker at points of care, thus supporting the timely diagnosis of heart failure., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2024
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2. Objective determination of peripheral edema in heart failure patients using short-wave infrared molecular chemical imaging

Author

Robert Schweitzer, Arjun S. Bangalore, Reina Perez, Jeffrey A. Cohen, Heather Gomer, Aaron G. Smith, Shona Stewart, Sandhya Vasudevan, Marlena Darr, J. Christopher Post, Aaron Thomas, Arash Samiei, Srinivas Murali, and Patrick J. Treado

Subjects
Paper, peripheral edema, Population, Biomedical Engineering, Peripheral edema, Biomaterials, molecular chemical imaging, Edema, medicine, Humans, Short wave infrared, In patient, Least-Squares Analysis, General, education, Heart Failure, education.field_of_study, business.industry, Discriminant Analysis, short-wave infrared, artificial intelligence, medicine.disease, Pitting edema, Atomic and Molecular Physics, and Optics, Molecular Imaging, Electronic, Optical and Magnetic Materials, Peripheral, Heart failure, medicine.symptom, business, Nuclear medicine
Abstract

Significance: Peripheral pitting edema is a clinician-administered measure for grading edema. Peripheral edema is graded 0, 1 + , 2 + , 3 + , or 4 + , but subjectivity is a major limitation of this technique. A pilot clinical study for short-wave infrared (SWIR) molecular chemical imaging (MCI) effectiveness as an objective, non-contact quantitative peripheral edema measure is underway. Aim: We explore if SWIR MCI can differentiate populations with and without peripheral edema. Further, we evaluate the technology for correctly stratifying subjects with peripheral edema. Approach: SWIR MCI of shins from healthy subjects and heart failure (HF) patients was performed. Partial least squares discriminant analysis (PLS-DA) was used to discriminate the two populations. PLS regression (PLSR) was applied to assess the ability of MCI to grade edema. Results: Average spectra from edema exhibited higher water absorption than non-edema spectra. SWIR MCI differentiated healthy volunteers from a population representing all pitting edema grades with 97.1% accuracy (N = 103 shins). Additionally, SWIR MCI correctly classified shin pitting edema levels in patients with 81.6% accuracy. Conclusions: Our study successfully achieved the two primary endpoints. Application of SWIR MCI to monitor patients while actively receiving HF treatment is necessary to validate SWIR MCI as an HF monitoring technology.

Published
2021