1. Genome-Wide Characterization and Expression Profiling of NBS-LRR-Encoding Gene Family in Radish (Raphanus sativus L.).
- Author
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Xu, Liang, Zhang, Wei, Tang, Mingjia, Zhang, Xiaoli, Wang, Juanjuan, Wang, Yan, and Liu, Liwang
- Subjects
RADISHES ,GENE expression profiling ,GENE families ,GENE expression ,ROOT crops ,PROMOTERS (Genetics) - Abstract
Radish (Raphanus sativus L.) is an important root vegetable crop that is easily infected by various pathogens that result in decreased yield and quality. Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes play vital roles in resisting pathogen infection in plants. However, the genome-wide characterization and functional roles of NBS-LRR genes remain largely unexplored in radish. Here, a total of 187 RsNBS-LRR genes were identified at the whole-genome level in radish, among which 80 RsNBS-LRR genes were unevenly distributed on nine radish chromosomes. Interestingly, 15 clusters containing 36 RsNBS-LRR genes occurred in eight chromosomes. RNA-Seq data showed that several RsNBS-LRR genes exhibited significant differential expression profiles in different radish tissues. Moreover, a range of cis-acting regulatory elements associated with ABA, MeJA, or SA were identified in the promoter region of some RsNBS-LRR genes. RT-qPCR analysis showed that the expression of a few RsNBS-LRR genes (e.g., RsNBS021 and RsNBS163) was significantly induced under Peronospora parasitica infection and/or ABA treatment, indicating that they might play critical roles in ABA-dependent defense resistance processes. These results could enhance our understanding of the evolutionary relationship of RsNBS-LRR genes and facilitate the genetic manipulation of disease resistance in radish breeding programs. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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