1. Probing the Tl+-induced structural transformation of G-rich DNA to G-quadruplex with a label‐free thioflavin T-based biosensor.
- Author
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Dong, Zhen, Xie, Chengxia, Li, Xianming, and Wu, Peng
- Subjects
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QUADRUPLEX nucleic acids , *THIOFLAVINS , *DNA , *OCHRATOXINS , *BIOSENSORS , *SMALL molecules , *CIRCULAR dichroism - Abstract
More than 700,000 G-rich sequences in the human genome are able to form G-quadruplex (G4). Since G4 is closely relevant to a variety of essential biological regulatory processes, identification of these G-rich DNAs (G-rich → G4) is thus important for DNA-involved interactions, e.g., metal ions and small molecules. Circular dichroism (CD) and NMR are the dominated tools for studying G4 DNA-based interactions, but suffers from low sensitivity. Herein, with toxic Tl+ as the model inducer, we demonstrated that thioflavin T (ThT), a typical label-free G4 dye, could be explored for highly sensitive probing of Tl+-induced structural transformation of G-rich DNAs to G4. With several model G4-forming DNAs, it was found that the Tl+-induced CD spectra developments correlated well with its fluorescence modulation on ThT (ThT-DNA → ThT-DNA-Tl+). In other words, the Tl+-induced structural change (Free State → G4) could be well probed with the ThT fluorescence change. For typical non-G4-forming DNAs, no appreciable ThT fluorescence modulation was received. To further increase the accuracy of picking up G4-forming DNAs from unknown samples, the photophysical parameters (absorption, fluorescence emission, and fluorescence lifetime) change of ThT before and after Tl+ modulation were integrated to develop a two-step linear discrimination analysis (LDA) protocol, with cross-validation accuracy of over 90%. After picking up G4-forming DNAs from the unknown samples, the Tl+-induced structural transformation of G4 DNAs could be thus evaluated. [Display omitted] • A G-quadruplex-based label-free fluorescent biosensor for probing of the G-quadruplex-involved interactions. • Much higher sensitivity of ThT-based fluorescent biosensor as compared with CD detection. • For unknown samples, first picking up G4 DNAs and then evaluating the Tl+-induced structural transformation of G4 DNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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