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Emerging evidence has shown that aberrant expression of lncRNA-TP53TG1 plays important roles in various malignancies. However, the biological functions of lncRNA-TP53TG1 in hepatocarcinogenesis, as well as the underlying mechanisms, remain largely unknown. Here, we assessed whether lncRNA-TP53TG1 plays a key role in the progression of hepatocellular carcinoma (HCC). The expression of lncRNA-TP53TG1 was significantly decreased in HCC tissues and cells. Decreased expression of lncRNA-TP53TG1 was associated with aggressive clinical phenotypes and a poor prognosis. Ectopic expression of lncRNA-TP53TG1 inhibited hepatoma cell proliferation and migration in vitro and in vivo, whereas lncRNA-TP53TG1 knockdown exerted the opposite effects. Furthermore, lncRNA-TP53TG1 played an important role in slowing the epithelial-mesenchymal transition (EMT) process in HCC. Mechanistically, lncRNA-TP53TG1 physically interacted with PRDX4 and promoted its ubiquitin-mediated degradation, resulting in the inactivation of the WNT/β-catenin signaling pathway in hepatoma cells. Our findings demonstrate a novel mechanism by which lncRNA-TP53TG1 exerts its tumor-suppressive effects through the WNT/β-catenin signaling pathway in a PRDX4-mediated manner in HCC. Based on these results, lncRNA-TP53TG1 potentially represents a prognostic indicator and therapeutic target for patients with HCC. [ABSTRACT FROM AUTHOR]

Nickel sulfides are perfect anode materials for high-capacity and low-cost lithium-ion batteries (LIBs); however, with the shortcoming of polysulfide intermediate dissolution, volume expansion exceeding the limit during cycling also restricts their development. Herein, NiS/C composite materials are successfully anchored on chestnut shell fluff (CSF)-derived biochar by a glucose-auxiliary hydrothermal method along with an annealing treatment. The CSF biochar acts as an effective electron transmission channel for the rapid lithiation/delithiation of NiS and as a fixed sulfur carrier for inhibiting the dissolution of polysulfide. Glucose restrains the accumulation of NiS particles and then transforms into uniform amorphous carbon during annealing, which is more effective in buffering for rapid volume variation. Moreover, the CSF-NiS/C electrode exhibits a remarkable specific capacity of 1522.8 mAh g-1 (0.1 A g-1) and distinguished rate performance with 295 mAh g-1 capacity (3 A g-1), which are better than those of the pure NiS/C anode material displays. Researchers may be inspired by both of these reasonable design and synthesis strategies that are beneficial for the development of high-performance nickel-based sulfide anode materials for LIBs. [ABSTRACT FROM AUTHOR]

Background Context: Many risk factors for osteoporotic vertebral compression fractures (OVCFs) have been reported. However, there are few reports on the relationship between spine sagittal parameters in patients with osteoporosis.Purpose: To explore whether: spinal sagittal imbalance is associated with future vertebral compression fractures in osteoporosis patients; spinal sagittal parameters in patients with osteoporosis can predict the occurrence of vertebral compression fractures.Study Design: A retrospective cohort study.Patient Sample: Patients with osteoporosis.Outcome Measures: Occurrence of OVCFs during the follow-up period.Methods: From January 2017 to October 2019, eligible patients with osteoporosis at the initial visit were enrolled. They were followed up to November 1, 2020. Based on whether OVCFs occurred during the follow-up, the patients were divided into two groups: the experimental group (vertebral compression fracture group) and the control group (no vertebral compression fracture group). Intragroup analysis was performed as follows: Pearson and Spearman correlation coefficients were used to calculate the correlation between each parameter. Intergroup analysis was performed as follows. For categorical variables, the chi-square test was used; for normally distributed continuous variables, an independent sample t-test was used; and for non-normally distributed variables, a two-sample nonparametric test was used. Binary logistic regression analysis and receiver operating characteristic (ROC) curves were used to determine independent risk factors and critical values, respectively.Results: A total of 340 patients with osteoporosis were enrolled. The longest and shortest follow-up periods were 44 months and 12 months, respectively, with an average of 25.2±10.2 months. There were significant differences in age, bone mineral density (femur and lumbar), smoking history, medication treatment regularity, Thoracolumbar Kyphosis (TLK), Pelvic Tilt (PT), C7-S1 Sagittal Vertical Axis (C7-S1 SVA), and C2-7 Sagittal Vertical Axis (C2-7 SVA) between the experimental and control groups. There were no significant differences in sex, body mass index (BMI), alcohol consumption history, hypertension, diabetes, coronary heart disease, family history of osteoporosis, physical activity, Thoracic Kyphosis (TK), Lumbar Lordosis (LL), Pelvic Incidence (PI), Sacral Slope (SS), C2-C7 Cobb Angle (CL), T1 slope (T1S) or blood parameters. Through binary logistic regression analysis, we found that BMD, medication treatment regularity and C7-S1 SVA were independent risk factors for future vertebral compression fractures. According to the ROC curve, the prediction accuracy of C7-S1 SVA was the highest. Through the calculation of critical values, we found that when C7-S1 SVA was more than 3.81 cm, future OVCFs were more likely to occur, and for every 1cm increase in C7-S1 SVA, the incidence of future OVCFs would increase by 0.324 times (p<.001, OR=1.324). Through intragroup analysis, we further found that C7-S1 SVA was positively correlated with the percentage of vertebral body wedging.Conclusions: For patients with osteoporosis, a C7-S1 SVA more than 3.81cm is significantly associated with a greater risk for vertebral compression fractures in the future. [ABSTRACT FROM AUTHOR]

[Display omitted] • Bioavailability of two typical shellfish species were performed using in vitro method. • Bioavailability of Zn and Cd in cooked C. rivularis samples both exceeded 82%. • Bioavailability of Zn and Cd in cooked P. undulata samples both exceeded 83%. • The intake of Cd from both shellfish species is safety. Dynamic extraction of edible tissues of Jinjiang oyster (Crassostrea rivularis) and Flower clam (Paphia undulata) was conducted using a fully biomimetic digestion (in vitro) method. The impact of different cooking methods on the bioavailability of Zn and Cd in the edible shellfish tissues was analyzed, and the human health risk of Zn and Cd was evaluated. The results show that the gastric biomimetic extractions of Zn and Cd in unheated samples of C. rivularis and P. undulata were higher than those in the intestinal biomimetic extraction. The extraction patterns of cooked samples were consistent with those of raw samples. The bioavailability of Zn and Cd in cooked samples of C. rivularis was 94.9% and 82.5%, respectively, indicating increased Zn bioavailability but decreased Cd bioavailability compared to the raw samples. The bioavailability of Zn and Cd in cooked samples of P. undulata was 85.1% and 83.0%, respectively, both of which decreased compared to the raw samples. Consumption of C. rivularis can provide 21.0% to 34.2% of the daily required Zn intake, while consumption of P. undulata can provide 3.8% to 6.4%. The intake of Cd from both shellfish species is below the monthly tolerable intake recommended by FAO/WHO. Consuming cooked C. rivularis can increase the intake of Zn and decrease the intake of Cd. [ABSTRACT FROM AUTHOR]

[Display omitted] • Bioavailability of two typical shellfish species were performed using in vitro method. • Bioavailability of Zn and Cd in cooked C. rivularis samples both exceeded 82%. • Bioavailability of Zn and Cd in cooked P. undulata samples both exceeded 83%. • The intake of Cd from both shellfish species is safety. Dynamic extraction of edible tissues of Jinjiang oyster (Crassostrea rivularis) and Flower clam (Paphia undulata) was conducted using a fully biomimetic digestion (in vitro) method. The impact of different cooking methods on the bioavailability of Zn and Cd in the edible shellfish tissues was analyzed, and the human health risk of Zn and Cd was evaluated. The results show that the gastric biomimetic extractions of Zn and Cd in unheated samples of C. rivularis and P. undulata were higher than those in the intestinal biomimetic extraction. The extraction patterns of cooked samples were consistent with those of raw samples. The bioavailability of Zn and Cd in cooked samples of C. rivularis was 94.9% and 82.5%, respectively, indicating increased Zn bioavailability but decreased Cd bioavailability compared to the raw samples. The bioavailability of Zn and Cd in cooked samples of P. undulata was 85.1% and 83.0%, respectively, both of which decreased compared to the raw samples. Consumption of C. rivularis can provide 21.0% to 34.2% of the daily required Zn intake, while consumption of P. undulata can provide 3.8% to 6.4%. The intake of Cd from both shellfish species is below the monthly tolerable intake recommended by FAO/WHO. Consuming cooked C. rivularis can increase the intake of Zn and decrease the intake of Cd. [ABSTRACT FROM AUTHOR]

[Display omitted] • The poor utilization of sucrose limits the CM application in Schizochytrium sp. • Overexpression of SH enhanced the utilization of sucrose by 2.57-fold. • The DHA titer of evolved strain from CM was 42.69% higher than from glucose. • Proteomics confirmed that CM enhanced DHA biosynthesis and lipid storage. • 25.26 g/L DHA with 51.45% content were obtained from CM by fed-batch. Cane molasses (CM) is a sugar-rich agro-industrial byproduct. The purpose of this study is to synthesize docosahexaenoic acid (DHA) in Schizochytrium sp. by using CM. The single factor analysis showed that sucrose utilization was the main factor limiting the utilization of CM. Therefore, the endogenous sucrose hydrolase (SH) was overexpressed in Schizochytrium sp., which enhanced the sucrose utilization rate 2.57-fold compared to the wild type. Furthermore, adaptive laboratory evolution was used to further improve sucrose utilization from CM. Comparative proteomics and RT-qPCR were used out to analyze the metabolic differences of evolved strain grown on CM and glucose, respectively. Finally, a constant flow rate CM feeding strategy was implemented, whereby the DHA titer and lipid yield of the final strain OSH-end reached 25.26 g/L and 0.229 g/g sugar, respectively. This study demonstrated the CM is a cost-effective carbon source for industrial DHA fermentation. [ABSTRACT FROM AUTHOR]

Parabens are widely added to food, cosmetics, and medicines as preservatives and are typical contaminants of pharmaceuticals and personal care products (PPCPs). However, their fate and transport in urban watersheds remain largely unexplored. This study investigated the role of road runoff as a critical nonpoint source of parabens and their metabolites in urban rivers based on 73 multimedia (road runoff and dust in different urban land uses, wastewater, stormwater discharge and river water) samples collected from a highly urbanized drainage area. Seven parabens and five metabolites were detected in the road runoff, with mean concentrations of ∑parabens and ∑metabolites equal to 47.5 ng/L and 4710 ng/L, respectively. The concentrations in road runoff were comparable to those in treated wastewater and river water and showed a land use pattern of residential > industrial > commercial. A first flush effect of the contaminants was observed in a heavy rainfall event with an antecedent dry period. In general, the population-based and area-based emission intensities of ∑parabens and ∑metabolites in road runoff were one order of magnitude higher than those in wastewater effluent during the rainfall events. This study provides quantitative evidence that road runoff can be a major pollution source of parabens and their metabolites in rapidly growing cities during the wet season and calls for the integrated management of nonpoint sources to prevent urban river contamination by typical PPCPs. [Display omitted] • The concentration of parabens in road runoff is comparable to that in WWTP effluent. • The areal emission intensity of parabens in road runoff exceeds that in effluent. • Residential areas have a higher level of runoff pollution by parabens than others. • Control of nonpoint sources is relevant to prevention of river pollution by parabens. [ABSTRACT FROM AUTHOR]

The increasing complexity of reservoirs, particularly shale reservoirs, demands accurate information on the formation composition, mineralogy, and mechanical parameters for effective exploitation. The development of geochemical and geophysical logging technology allows the investigation of the correlations between the elemental, mineral contents, and the mechanical parameters of shale. Taking the Lower Cambrian Niutitang Formation shale in the Fenggang block in northeastern Guizhou as an example, 889 data sets of the main elemental (Si, Ca, Fe, S, Ti, Gd, K, Mg, and S) concentrations and minerals (carbonate, pyrite, clay, quartz, feldspar, and mica) from Elemental Capture Spectroscopy logging and rock mechanics parameters (dynamic Young's modulus, dynamic Poisson's ratio, shear modulus, bulk modulus, and brittleness index) determined via Sonic Scanner logging data in the target zone of the study area are collected. We investigated the relationships between the mechanical parameters and the elemental, and mineral concentrations by Spearman rank correlation analysis. The influence of different Si concentrations on the mechanical parameters of the rock (positive/negative, and significance) is discussed based on the mineral contents determined via X-ray diffraction of 24 experimental samples. When 22.1% < DWSi (dry weight fraction of the silicon content as a percent) < 28.40% and 28.40% < DWSi <34.20%, the static Young's modulus, shear modulus, bulk modulus, and brittleness index exhibit a highly positive/negative correlation that is significant with the increase/decrease in DWSi, WCLA (dry weight fraction of clay), and WQFM (dry weight fraction of quartz, feldspar, and mica) contents, respectively. DWSi = 28.4% is suggested as the turning point for the correlation change (positive/negative, significance). Thus, there exists uncertainty in the determination of the mechanical parameters of rock within certain elemental content ranges. More mathematical models and factors need to be considered to adequately characterize the mechanical parameters of rock. • Rock's elemental and mineral and mechanical parameters characterization in the Lower Cambrian Niutitang Formation shale. • Spearman rank analysis on elements, minerals and mechanical parameters of a rock. • Discuss the degree of Spearman rank correlations regarding elements and minerals on mechanical parameters. • Discuss the possible mineral sources of silicon in different ranges. [ABSTRACT FROM AUTHOR]

Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat accumulation in the body. We found that miR-370-3p was expressed at lower levels in the fat mass of mice on a high-fat diet than in mice on a normal control diet. Furthermore, our data showed that the overexpression of miR-370-3p significantly suppressed the mRNA expression levels of adipogenic markers. Thus, miR-370-3p overexpression reduced lipid accumulation. Conversely, the inhibition of miR-370-3p suppressed 3T3-L1 preadipocyte proliferation and promoted preadipocyte differentiation. In addition, Mknk1, a target gene of miR-370-3p, plays an opposing role in preadipocyte proliferation and differentiation. Moreover, consistent results from in vitro as well as in vivo experiments suggest that the inhibition of fat accumulation by miR-370-3p may result from the inhibition of saturated fatty acids that promote the accumulation of polyunsaturated fatty acids. In conclusion, these results suggest that miR-370-3p plays an important role in adipogenesis and fatty acid metabolism through the regulation of Mknk1. [ABSTRACT FROM AUTHOR]

In recent years, frequent severe haze weather has formed in China, including some of the most populated areas. We found that these smog-prone areas are often relatively a "local climate" and aim to explore this series of scientific problems. This paper uses remote sensing and data mining methods to study the correlation between haze weather and local climate. First, we select Beijing, China and its surrounding areas (East longitude 115°20′11″–117°40′35″, North latitude 39°21′11″–41°7′51″) as the study area. We collected data from meteorological stations in Beijing and Xianghe from March 2014 to February 2015, and analyzed the meteorological parameters through correlation analysis and a grey correlation model. We study the correlation between the six influencing factors of temperature, dew point, humidity, wind speed, air pressure and visibility and PM2.5, so as to analyze the correlation between haze weather and local climate more comprehensively. The results show that the influence of each index on PM2.5 in descending order is air pressure, wind speed, humidity, dew point, temperature and visibility. The qualitative analysis results confirm each other. Among them, air pressure (correlation 0.771) has the greatest impact on haze weather, and visibility (correlation 0.511) is the weakest. [ABSTRACT FROM AUTHOR]