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Objective To investigate the relationship of miRNA gene polymorphisms with blood pressure (BP) responses to the sodium and potassium diet intervention. Methods In 2004, we recruited 514 participants from 124 families in seven villages of Baoji, Shaanxi Province, China. All subjects were given a three-day normal diet, followed by a seven-day low-salt diet, a seven-day high-salt diet, and finally a seven-day high-salt and potassium supplementation. A total of 19 miRNA single nucleotide polymorphisms (SNPs) were selected for analysis. Results Throughout the sodium-potassium dietary intervention, the BP of the subjects fluctuated across all phases, showing a decrease during the low-salt period and an increase during the high-salt period, followed by a reduction in BP subsequent to potassium supplementation during the high-salt diet. MiR-210-3p SNP rs12364149 was significantly associated with systolic BP (SBP), diastolic BP (DBP) and mean arterial pressure (MAP) responses to low-salt diet. MiR-4638-3p SNP rs6601178 was significantly associated with SBP while miR-26b-3p SNP rs115254818 was significantly associated with MAP responses to low-salt intervention. In addition, miR-26b-3p SNP rs115254818 was significantly correlated with SBP, DBP and MAP responses to high-salt intervention. MiR-1307-5p SNPs rs11191676 and rs2292807 were associated with SBP and MAP responses to high-salt diet. MiR-4638-3p SNP rs6601178, miR- 210-3p SNP rs12364149, miR-382-5p SNP rs4906032 and rs4143957 were significantly associated with SBP response to high-salt diet. In addition, miR-26b-3p SNP rs115254818 was significantly associated with SBP, DBP and MAP responses to potassium supplementation. MiR-1307-5p SNPs rs11191676, rs2292807, and miR-19a-3p SNP rs4284505 were significantly associated with SBP responses to high-salt and potassium supplementation. Conclusion miRNA gene polymorphisms are associated with BP response to sodium and potassium, suggesting that miRNA genes may be involved in the pathophysiological process of salt sensitivity and potassium sensitivity. [ABSTRACT FROM AUTHOR]

目的 探讨基于整体责任制的临床护理路径(CNP)干预对 CT 增强检查患者心理状况及检查 配合度的影响. 方法 选取为2021年1月至2023年4月该院CT 增强检查患者88例,以随机数字表法按1∶ 1比例分为对照组和观察组,各44例. 对照组接受常规护理干预,观察组接受基于整体责任制的 CNP干预,比 较2组广泛性焦虑障碍量表(GAD-7), 9项患者健康问卷抑郁量表(PHQ-9)评分,以及检查配合度和满意度. 结果 2组干预前 GAD-7, PHQ-9评分比较,差异无统计学意义(P>0.05). 干预后,2组 GAD-7, PHQ-9评 分低于干预前,且观察组低于对照组,差异有统计学意义(P<0.05). 观察组检查配合度高于对照组,差异有统 计学意义(P<0.05). 观察组满意度为97.73%(43/44),高于对照组的81.82%(36/44),差异有统计学意义 (χ2=4.456,P=0.035). 结论 基于整体责任制的 CNP干预可改善 CT 增强检查患者不良心理,提高其检查 配合度及满意度. [ABSTRACT FROM AUTHOR]

Objective To explore the status quo of stress-related growth of caregivers of children with congenital pseudarthrosis of the tibia (CPT) and examine its possible influencing factors. Methods For this retrospective study, Caregivers of CPT children admitted into Hunan Children's Hospital from January to March 2023 were selected as study subjects. Simplified coping behavior questionnaire, social support rating scale, simplified Chinese version of post-traumatic growth rating scale and general profile questionnaire were utilized. Multiple linear regression and Pearson's correlation analyses were performed for examining the major influencing factors of caregiver stress-related growth in CPT children. Results Among a total of 161 questionnaires distributed, 155 were valid with an effective rate of 96.27%. Total stress-related growth score was (60.86±16.13). Pearson's correlation analysis indicated that all dimensions of stress-related growth and total scores of caregivers were correlated positively with positive coping styles (P<0.05). Besides self-transformation dimension, all dimensions of stress-related growth and total scores were correlated positively with social support style (P<0.05). According to multiple linear regression, children's age, caregiver occupation (whether employed or not), active coping and social support were the major influencing factors of stress-related growth of caregivers of CPT children (P<0.05, adjusted R²=0.443). Conclusions The stress-related growth of caregivers of CPT children is at a moderate level. Active coping, social support, children's age and caregiver occupation are the major factors affecting the stress-related growth level of caregivers of CPT children. Therefore, in clinical practices, attention should be paid to caregivers' coping styles and social support. The corresponding intervention measures may be formulated according to the above influencing factors to enhance their stress-related growth level and thus improve the outcomes of children. [ABSTRACT FROM AUTHOR]

The global population has entered a stage of ageing, and the incidence of frailty is increasing year by year. Frailty is not only associated with dysfunction of multiple systems, but also with mitochondrial dysfunction, chronic inflammation, oxidative stress and malnutrition. Among them, malnutrition is considered to be a key factor in its pathophysiological changes. As malnutrition is a modifiable risk factor for frailty, early nutritional interventions can be used to prevent frailty and improve the quality of life of the frailty elderly. [ABSTRACT FROM AUTHOR]

Objective To analyze the pesticriele; residues in vegetables sold in Hubei Province, and to evaluate; the pesticide; residue; risks. Methods 1 327 samples of 8 types of vegetables were; collected in Hubei Province; in 2022. 46 pesticide; residues were; detected and analyzed, and the; indc;x of foed safety method was used to assess risks. Results 35 were; ele;te;e;te;ei and 16 exceeded the; standard among the; 46 pesticides. The; highest detection rate; was cnoylmorpholinc; (53.09%) anel the; highest exceeding rate; was triazophos (22. 22%). 7 kinds of vegetables were; detected, anel 5 kinds of vegetables exceeded the; stanelarel among the; 8 kinels of vegetables. The; highest detention rate; was melon (65. 79%) anel the; highest exceeding rate; was bean (32. 53%). The; safety index of the; average; value; erf elete;e;te;el pesticide; rcsielucs was less than 1, anel the; safety index of the; maximum value; erf se;ve;n pesticide; residues, such as fipronil, triazophos was more; than 1. Conclusion The; overall risk of pesticide; residues in vegetables sold in Hubei Province; is relatively low in 2022, but some; vegetable; varieties have; a high rate; erf' exceeding the; stanelarel. The; maximum detected values erf pesticide; residues sucli as flufenitrile;, triazophos pose; certain safety risks. [ABSTRACT FROM AUTHOR]

In recent years, an increasing number of researchers have attempted to link catalytic activity to the acidity and basicity of the oxygen vacancies in catalysts. A deeper understanding of the role played by oxygen vacancy defects in determining catalytic properties and charge carrier dynamics by combining Lewis acid-base active sites can provide valuable insights for the rational development of more efficient catalysts. Oxygen vacancies are the most common defect in transition metal oxides, and catalysts containing oxygen vacancies are closely related to how surface acidity and basicity affect the adsorption, reaction, and desorption processes of reactants, intermediates, and solid surface products. To this end, the location and the acid-base of oxygen vacancies have always played a central role in the history of multiphase catalysis, and the study of the construction of Lewis acid-base sites based on oxygen vacancy defects in catalysts is of great significance for the performance enhancement of various molecular activation and catalytic conversion reactions. The surface properties of multiphase catalysts will largely determine the electronic structure of the surface active sites. Therefore, this paper reviews the research on the oxygen vacancy-containing solid catalysts with Lewis acid-base sites, explores the effects of the corresponding active sites on the catalytic efficiency, stability and chemoselectivity of the catalysts, and summarizes their relevant applications in the field of photocatalysis. [ABSTRACT FROM AUTHOR]

[Objective]To isolate and screen the high-efficiency cellulose degrading bacteria from traditional Chinese medicine residue, and to determine the cellulase activity in order to improve the added value of traditional Chinese medicine residue. [Method]The decomposition products of traditional Chinese medicine residues and soil samples piled up with drug residues for a long time were enriched and cultured in Hutchison medium, and the strains with drug residues as the only carbon source were isolated and purified. The obtained strains were primary screened by Congo red plate method, secondary screening by determine the strain’ s filter paper activity, exo-β-glucanase activity, endo-βglucanase activity and glucosidase activity. The strains were identified by morphology, physiology and biochemistry, 16S rDNA sequence analysis. [Result]One cellulose degrading strain YZ25 with good cellulase production was screened, with FPA enzyme activity of 25. 073 U/ mL, CMC enzyme activity of 24. 978 U/ mL, CBH enzyme activity of 58. 452 U/ mL, and CB enzyme activity of 37. 202 U/ mL. The strain YZ25 was identified as Bacillus subtilis. [Conclusion] This study enriches the resource pool of cellulose degrading strains from drug residues and has a good application prospect, which has practical significance for the research and development of functional biological organic fertilizer from traditional Chinese medicine residue. [ABSTRACT FROM AUTHOR]

To investigate the potential targets and related mechanisms of micheliolide (MCL) in alleviating gouty arthritis (GA). The network pharmacology technology was used to retrieve MCL and GA related targets, screen their intersection targets, construct protein interaction maps and screen core targets. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was conducted, the ″MCL-target-pathway-GA″ network diagram was drawn, and molecular docking verification of MCL and core target was conducted. Results A total of 778 MCL related targets, 351 GA related targets and 58 intersection targets were screened out. Twenty-four targets were predicted to be the core targets of MCL inalleviating GA, such as TNF, IL-1B (IL-1β) and so on. GO analysis confirmed 294 items related to biological process (BP), 38 items related to cellular components (CC) and 57 items related to molecular function (MF). KEGG analysis revealed 105 signaling pathways, including inflammation, metabolism, infection and tumor. The molecular docking results showed that MCL binds tightly to the core target of TNF and IL-1B (IL-1β) by hydrogen bonding, and the MCL binds most tightly to the core target of IL-1B (IL-1β). The core targets and related biological processes and signaling pathways of MCL alleviating GA were preliminarily predicted, and molecular docking verification was carried out, providing ideas for further in vivo and in vitro experiments. [ABSTRACT FROM AUTHOR]

Objective Corin, a transmembrane serine protease that can cleave atrial natriuretic peptide precursor (pro-ANP) into atrial natriuretic peptide with smaller bioactive molecules, participates in the pathophysiologicalprocess of hypertension and cardiac hypertrophy. The purpose of this study was to explore the relationship of Corin gene variation with blood pressure responses to sodium and potassium dietary interventions. Methods In 2004, we recruited 514 participants from 124 families in 7 villages of Baoji, Shaanxi Province, China. All the subjects received a 3-day normal diet, a 7-day low-salt diet, a 7-day high-salt diet, and finally a 7-day high-salt and potassium supplementation. Fifteen single nucleotide polymorphisms (SNPs) of Corin gene were selected for final analysis. Results SNPs rs12509275 were significantly associated with diastolic blood pressure (DBP) response to low-salt diet, while rs3749584 was associated with pulse pressure (PP) response to low-salt diet.SNP rs3749584 and rs10517195 were significantly associated with PP response to high-salt diet. In addition,rs17654278 were significantly associated with systolic blood pressure (SBP) response to high-salt and potassium supplementation, rs2271037 was significantly correlated with DBP responses to high-salt and potassium supplementation, and rs4695253, rs12509275, rs2351783, rs36090894 were significantly associated with PP response to high-salt and potassium supplementation. Conclusion Corin gene polymorphisms were associated with blood pressure response to sodium and potassium, suggesting that Corin gene may be involved in pathophysiological process of salt sensitivity and potassium sensitivity. [ABSTRACT FROM AUTHOR]

A nonlinear switching control method was proposed based on an improved quasilinear autoregressive with exogenous inputs (quasi-ARX) radial basis function (RBF) network model and the support vector regression (SVR) algorithm. The RBF network was chosen as the nonlinear part of the improved quasi-ARX prediction model. The proposed controller design method was divided into 3 steps: firstly, the nonlinear parameters of the model were determined with the clustering method; secondly, the linear SVR algorithm was used to solve the robustness problem of the control system; thirdly, the switching criterion function was given based on the control error, and the control sequence were determined according to the switching law. Finally, a numerical example was given to verify the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

To research the effect of Chinese (AOHUA AQ-100 type) endoscope on the diagnosis of heterotopic gastric mucosa in the upper esophagus (HGMUE) under anesthesia.Methods The patients who underwent endoscopy in the endoscopy center of a hospital from January 2018 to March 2020 were selected as the research objects and randomly divided into the experimental group and the control group.The AOHUA AQ-100 type gastroscope (AQ-100-type medical endoscopic image processor and AQL-100-type xenon cold light source) was used in the experimental group.while the Olympus gastroscope (GIF-Q260J-type) was used in the control group.A total of 2 000 cases were examined in each group respectively.The patients were divided into the painless test group and the general test group for examination on a voluntary basis.In each group,moderate air injection observation was started at about 15 cm from the incisor,and then full air injection was started at about 25 cm from the incisor while slowly withdrawing the gastroscope (from about 25cm from the incisor to about 15cm from the incisor for more than 30 seconds),and careful observation was carried out.When the orange-red lesion with a clear boundary was observed,its position,size,shape and quantity were recorded,after considering it as HGMUE.Then,one to two pieces of mucosa were biopsied in the center of the suspected lesion and sent for pathological examination.The diagnosis was made according to the pathological results.Results In the experimental group,the total detection positive rate of HGMUE was 1.80% (36/2 000).Among them,the detection positive rate was about 1.35% (14/1 030) in the ordinary gastroscope group and 2.29% (22/970) in the painless gastroscope group.In the control group,the total detection positive rate of HGMUE was 2.00% (40/2 000).Among them,the detection positive rate was about 1.67% (13/780) in the ordinary gastroscope group and 2.21% (27/1 220) in the painless gastroscope group. In this study,there was no significant statistical difference in the total detection positive rate of HGMUE between the experimental group and the control group (P >0.05).There were no significant statistical differences about the positive rate in the painless gastroscope groups and the ordinary group between the experimental group and the control group too.Conclusion The application of the AOHUA AQ-100 medical gastroscope has the advantages of moderate price,clear image and good performance,which is helpful to promote the popularization of endoscopy in our country,especially in painless gastroscopy.It can improve the diagnosis rate of HGMUE. [ABSTRACT FROM AUTHOR]

Using Yuanjia Village in Liquan County, Shaanxi Province, China as a case study, this paper uses qualitative research methods to analyze the place identity construction of rural in‐ coming tourism entrepreneurs from the perspective of everyday life. The results show that the in‐ coming tourism entrepreneurs in Yuanjia Village have a strong emotional attachment to rural pastoral life, which is expressed through the everyday life worlds of rural areas consisting of every‐ day livelihood, everyday communication and everyday concepts. In the everyday life worlds of rural areas, the incoming tourism entrepreneurs are continuously shaping the life imagination and identity cognition after escaping from urban modernity. With the increase of everyday inter‐ active practice in the destination of incoming tourism entrepreneurs, identity will also be gradual‐ ly transformed and endowed with placeness, further stimulating the positive behavior of individu‐ als to the destination. [ABSTRACT FROM AUTHOR]

[Objective] To select oat varieties with high aphid resistance, strong production performance and excellent comprehensive performance, to provide theoretical references for the healthy development of the oat industry in Inner Mongolia. [Methods] In Shaerqin farming-pastoral test site of Institute of Grassland Research of Chinese Academy of Agricultural Sciences in Tumed Left Banner, Hohhot, taking 17 oat varieties as test materials, the aphid resistance was identified by aphid ratio method. The aphid resistance grade of each variety was determined. And the agronomic traits and yield were used as indicators to compare the differences in production performance of the varieties. Bivariate correlation analysis and systematic cluster analysis were performed using agronomic parameters, yield, and aphid ratio as indicators. [Results] The aphid resistance of the test oat varieties was categorized into 6 grades. Baiyan No.7, Mengyan No.3, Qinghai 444, and Qingyin No.2 had high resistance. Qinghaitian oats and Qingyan No.1 showed medium resistance. Mengsiyan No.2, Lingxiu, Beile, Taiyangshen, and Yellow Oats had low resistance. Bayan No.1 and Bayan No.7 were low susceptible. Bayan No.5 was medium susceptible. Aiwo126, Yanwang and Furuizhi were highly susceptible. The plant height of Baiyan No.7 and Taiyangshen reached over 1.18 m, which was significantly higher than that of Bayan No.7, Lingxiu, Yanwang, Qingyin No.2, Aiwo 126, Furuizhi (P<0.05). Beile had the highest fresh grass yield (4.34 kg/m2), which was significantly higher (P<0.05) than other varieties except Bayan No.5 and Aiwo 126. Taiyangshen had the highest hay yield (1.69 kg/m2), which was significantly higher (P<0.05)than that of Bayan No.7 and Qingyin No.2. There was no significant difference (P>0.05)in the fresh-to-dry ratio of the 17 oat varieties. Aiwo 126 had the highest (2.98) fresh-to-dry ratio, while Qinghai 444 had the lowest (1.86). The results of cluster analysis showed that the aphid ratio, plant height and yield performance of different oat varieties varied. The comprehensive performance of class I oat varieties (Qinghaitian oats, Mengyan No.3, Qinghai 444, Baiyan No.7, Lingxiu, Qingyan No.1, Yellow Oats, Taiyangshen, Bayan No.1, Bayan No.5, Bayan No.7, and Mengsiyan No.2) was stronger. [Conclusion] Qinghaitian oats, Mengyan No.3, Qinghai 444, Baiyan No.7, Lingxiu, Qingyan No.1, Yellow Oats, Taiyangshen, Bayan No.1, Bayan No.5, Bayan No.7 and Mengsiyan No.2 had strong aphid resistance, good production performance, and excellent comprehensive performance.They were suitable for planting in Tumed Left Banner in Inner Mongolia. [ABSTRACT FROM AUTHOR]

The experiment was conducted to study the effect of composite plant extracts Piper sarmentosum Roxb. and guava leaf on growth performance and serum indexes of weaned piglets. A total of 200 21-day-old weaned piglets were selected and randomly divided into two groups, with five replicates in each group and 20 piglets in each replicate. Piglets in control group were fed with a basal diet, the piglets in experimental group were fed with the basal diet supplemented with composite plant extracts at 200 mg/kg. The experimental period was 21 d. The results showed that there was no significant difference on growth performance of piglets among the groups（P>0.05）. Compared with control group, the experimental group supplemented with the compound plant extract in the diet significantly decreased the serum cholesterol（CHOL） and low-density lipoprotein cholesterol（LDL-C） content of piglets（P<0.05）, glutathione peroxidase（GSH-Px）activity was significantly increased（P<0.05）, serum content of interleukin-4（IL-4）, interleukin-10（IL-10） and transforming growth factor-β（TGF-β） were significantly increased（P<0.05）, the content of interleukin-1β（IL-1β）,interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） were significantly decreased（P<0.05）, and serum endotoxin（ET-1） content and diamine oxidase（DAO） activity were significantly decreased（P<0.05）. The study indicates that adding compound plant extracts to diet can improve the antioxidant performance and immune function of weaned piglets, and improve the intestinal mucosal barrier function of piglets. [ABSTRACT FROM AUTHOR]

[Objective] To use Nit mutant system to explore the genetic diversity of fragrant pear from different geographical sources in Xinjiang. [Method] In this study, Nit mutant technology (nitrate-resistant defective mutant) was used for the first time to study the nutritional affinity of Cytospora pyri, 13 strains of Korla pear tree rot from three different growing areas in southern Xinjiang. [Result] 115 Nit mutants were cultured in chlorate medium (KPS) from 13 strains, and 41 of them did not mutate. In the identification of mutant stability, 33 stable Nit mutants and 82 unstable Nit mutants were identified. The phenotype of the resulting Nit mutants was identified as NitD 85% and NitB 15%. [Conclusion] In this study, two Nit mutants were obtained, indicating that there may be two mutations in the nitrogen assimilation pathway of C. pyri. All isolates tested were able to utilize hypoxanthine, and the ability to use hypoxanthine ruled out the possibility that molybdenum cofactor sites were blocked. [ABSTRACT FROM AUTHOR]

目的探究超脉冲 CO2 点阵激光联合低浓度 5-氟尿嘧啶对烧伤后增生性瘢痕患者的影响。方法回顾性分析自 2019 年1 月至2021 年9 月，川北医学院附属医院整形美容烧伤外科收治118 例烧伤后增生性瘢痕患者，按治疗方法分为两 组，A 组采用低浓度 5-氟尿嘧啶单独治疗；B 组采用超脉冲 CO2 点阵激光联合低浓度 5-氟尿嘧啶治疗。治疗3 个月后比较两 组患者的临床总有效率、温哥华瘢痕量表（vancouver scar scale, VSS）、双向瘢痕评估量表（patient and observer scar assessment scale, POSAS）评分、视觉模拟评分法（visual analogue scale, VAS）、数字评分法（numeric rating scale, NRS）及不良反应发生情况。 结果两组患者均有治疗效果，B 组总有效率大于A 组（P<0.05）。治疗后患者瘢痕的厚度、颜色、柔软度、血管分布情况都有 明显的好转，且B 组的VSS 总评分明显低于A 组（P<0.05）。B 组POSAS、VAS、NRS 评分均低于A 组（P<0.05）。治疗过程中两组 患者不良反应发生情况比较，差异无统计学意义（P>0.05）。结论采用超脉冲 CO2 点阵激光联合低浓度 5-氟尿嘧啶治疗烧伤 后增生性瘢痕可以减轻患者疼痛、瘙痒症状，改善瘢痕状态，提高皮肤美观度，且该治疗方案安全性较高。 [ABSTRACT FROM AUTHOR]

In order to investigate the genetic diversity and variation of different body colors of common carp (Cyprinus carpio), a total of 150 individuals from six different colored koi carp populations (Platinum Ogon, Higoi, Kohaku, Tajsho-sanke, Shiro Utsuri and Hi Utsuri), two colored FFRC No.2 strain common carp (grey and red) populations, and two hybrid carp populations (grey FFRC No.2 strain common carp♀ × Kohaku koi♂,Kohaku koi♀ × grey FFRC No.2 strain common carp♂) were analyzed based on mtDNA D-loop sequences. The results showed that the contents of A, T, G and C in the 932 bp D-loop sequence were 33.2%, 32.8%, 14.2% and 19.8%, respectively. In total, 34 variant sites were detected in all individuals, showing 18 haplotypes. Among them, there were two dominant haplotypes in Kohaku and Hi Utsuri, respectively. There were two unique haplotypes in white koi carp and grey FFRC No.2 strain common carp, and grey and red FFRC No.2 strain common carp shared one dominant haplotype. The genetic distances across the ten populations ranged from 0.001 to 0.018, with FST values ranging from 0.011 to 0.933. The analysis of molecular variance (AMOVA) revealed that there was 67.17% variation among varieties and 32.83% variation within varieties, indicating highly substantial genetic difference. It demonstrated that the Hi Utsuri, white koi carp, and Tajsho-sanke had high genetic diversity, while the Shiro Utsuri, FFRC No.2 strain common carp (grey and red), and hybrid carp had high genetic purity. The study enriched the genetic data of the various body color carp germplasm resources and provided the guidance for germplasm resource utilization and genetic selection in the future. [ABSTRACT FROM AUTHOR]

Objective Dyslipidemia has shown to be associated with cardiovascular, metabolic and renal diseases. This study aimed to investigate the association between residual cholesterol and the risk of subclinical renal damage (SRD). Methods A total of 2 342 participants were recruited from the previously established Hanzhong Adolescent Hypertension Study cohort. According to estimated glomerular filtration rate(eGFR) and urinary albumin-to-creatine ratio(uACR), the subjects were divided into SRD group and non-SRD group. The associations of residual cholesterol with eGFR, uACR, and the risk of SRD were analyzed by multiple linear and Logistic regression analyses. Results Residual cholesterol was positively correlated with uACR(r=0.081, P<0.001) but negatively correlated with eGFR (r=-0.091, P<0.001). Multiple linear regression analysis revealed that residual cholesterol was an influencing factor of uACR (β=0.075, P<0.001) and eGFR (β=-0.027, P<0.001) after adjustment for gender, age, smoke, alcohol, exercise, BMI, hypertension, diabetes and serum uric acid. In addition, Logistic regression analysis revealed that residual cholesterol was significantly associated with the risk of SRD independently of potential confounders [OR(95% CI)=1.387 (1.113-1.728), P<0.001]. Further subgroup analysis showed that residual cholesterol was significantly associated with the risk of SRD in women but not in men. Conclusion Residual cholesterol is a contributing factor in the risk of subclinical renal damage with gender-specific association. [ABSTRACT FROM AUTHOR]

Objective Based on our previously established salt-sensitive hypertension cohort, we aimed to examine the association of genetic variants in uromodulin with blood pressure(BP) responses to dietary interventions of sodium and potassium intake. Methods In 2004, 514 subjects from 124 families in Mei County, Shaanxi Province, were recruited to establish the salt-sensitive hypertension study cohort. Among them, 333 non-parent subjects were selected and sequentially maintained on a normal-diet for 3 days, low-salt diet for 7 days, then a high-salt diet for 7 days and a high-salt diet with potassium supplementation for another 7 days. Thirteen single nucleotide polymorphisms(SNPs) in the uromodulin gene were genotyped on the MassARRAY platform. Results BP levels decreased from the baseline to low-salt diet, increased from low-salt to high-salt diet, and decreased again from the high-salt diet to the high-salt plus potassium supplementation intervention. SNPs rs77875418 and rs4997081 of the uromodulin gene were significantly associated with diastolic BP(DBP) and mean arterial pressure(MAP) responses to high-salt diet. In addition, SNPs rs77875418, rs79245268, rs4293393, rs6497476, rs4997081, rs13333226, and rs12917707 were significantly associated with systolic BP(SBP), DBP, and MAP responses to high-salt diet with potassium supplementation. Conclusion Genetic variants in uromodulin gene are significantly associated with BP responses to sodium and potassium supplementation, suggesting that uromodulin may be mechanistically involved in BP sodium-sensitivity and potassium-sensitivity. [ABSTRACT FROM AUTHOR]

Objective 4-like protein with down-regulated expression and development in neural precursor cells (NEDD4L) plays an important role in blood pressure (BP) regulation and sodium homeostasis by regulating epithelial sodium channel protein. In this study, we aimed to explore the relationship of NEDD4L gene polymorphisms with BP responses to sodium and potassium intake. Methods In 2004, 514 subjects from 124 families in Meixian County, Shaanxi Province, were recruited to establish a salt-sensitive hypertension study cohort. All the subjects received a 3-day baseline survey, a 7-day low-salt diet, a 7-day high-salt diet, and finally a 7-day high-salt and potassium supplementation. Their BP was measured and peripheral blood samples were collected at different intervention periods. The 14 gene polymorphisms of NEDD4L gene were genotyped and analyzed by MassARRAY platform. Results BP decreased on a low-salt diet, and significantly increased on a high-salt diet, and decreased again after potassium supplementation. NEDD4L SNPs rs74408486 were significantly associated with systolic BP, diastolic BP and mean arterial pressure responses to the low-salt diet. SNPs rs292449 and rs2288775 were significantly associated with pulse pressure response to the high-salt diet. In addition, SNPs rs563283 and rs292449 were significantly associated with diastolic BP, mean arterial pressure, and pulse pressure responses to high-salt and potassium supplementation diet. Conclusion NEDD4L gene polymorphisms were significantly associated with BP responses to sodium and potassium intake, suggesting that NEDD4L gene may be involved in the development of salt sensitivity and potassium sensitivity. [ABSTRACT FROM AUTHOR]

Objective M3 muscarinic acetylcholine receptor(M3 receptor), encoded by CHRM3 gene, is widely distributed in the cardiovascular system and plays an important role in cardiac regulation. The aim of this study was to assess the association of genetic variants in M3 receptor with blood pressure(BP) responses to controlleddietary sodium and potassium interventions. Methods A total of 333 subjects from 124 families were recruited from the rural areas of northern China. After a three-day baseline observation, they were sequentially on a seven-day low-salt diet, a seven-day high-salt diet, and a seven-day high-salt diet plus potassium supplementation. Thirteen CHRM3 single nucleotide polymorphisms(SNPs) were selected for analysis. Results SNP rs10802811 of the CHRM3 was significantly associated with diastolic BP(DBP) and mean arterial pressure(MAP) responses to both low-salt and high-salt diets while SNPs rs6429147, rs373288072, rs114677844 and rs663148 showed significant associations with systolic BP(SBP) and MAP responses to high-salt diet. In addition, SNP rs6692904 was significantly associated with SBP, DBP and MAP responses to high-salt diet with potassium supplementation. Conclusion Genetic variants in M3 receptor are significantly associated with BP responses to sodium and potassium intervention, suggesting that M3 receptor may be mechanistically involved in BP salt and potassium sensitivity. [ABSTRACT FROM AUTHOR]

BACKGROUND: For elderly patients with hip fractures, improving the ability to screen for deep vein thrombosis of the lower extremities and implementing individualized prevention programs are important measures to improve the prognosis of patients and reduce the burden on patients' families and socioeconomics. OBJECTIVE: To investigate the risk factors of deep venous thrombosis in elderly patients with hip fracture after operation, and to establish a risk model to predict the occurrence of deep venous thrombosis. METHODS: Clinical data of 325 hospitalized patients with hip fracture over 70 years old from January 2017 to December 2019 were retrospectively analyzed, and they were divided into deep venous thrombosis group (n=50) and non-deep venous thrombosis group (n=275) according to the results of deep vein color Doppler ultrasound examination of lower extremities. The univariate and multivariate Logistic regression analyses were used to identify the independent risk factors for postoperative deep venous thrombosis in elderly patients with hip fracture. Receiver operating characteristic curve was used to analyze the diagnostic efficacy of independent risk factors and combined models for deep venous thrombosis. Furthermore, R Studio software was used to construct a column chart to predict the risk of postoperative deep venous thrombosis of lower limbs in elderly patients with hip fracture, and calibration curves were made to verify the accuracy of the model. RESULTS AND CONCLUSION: (1) The univariate analysis results showed that age, fracture type, the time between injury to operation, anesthesia, operation time, preoperative basic diseases, potassium, cholesterol, creatinine, and total protein were significantly different between the two groups (P < 0.05). (2) Multivariate Logistic regression analysis showed that fracture type of intertrochanteric fracture of femur, time from injury to operation, presence of diabetes before operation, and cerebrovascular disease were independent risk factors for postoperative deep venous thrombosis in elderly patients with hip fracture (P < 0.05). (3) Receiver operating characteristic curve of each independent risk factor and combined model exhibited that the time from injury to operation, the presence of diabetes, cerebrovascular disease, and the combined model had predictive ability, but the type of hip fracture did not have predictive ability, and the combined model had better predictive ability than the single independent risk factor. (4) The column chart has good diagnostic efficiency and accuracy. [ABSTRACT FROM AUTHOR]

Objective To observe the effects of miR-128 and ZEB1 on the migration, invasion and epithelial mesen‐ chymal transition( EMT) of endometrial carcinoma( EC) cells and to verify the targeted relationship between them. Meth⁃ ods The qRT-PCR and Western blotting were used to detect the relative expression levels of miR-128 and ZEB1 in three EC cell lines( HEC-1A, Ishikawa, and KLE) and one endometrial epithelial cell line hEEC, respectively. Ishikawa cell line with the lowest expression level of miR-128 was taken as the follow-up study object. Ishikawa cells were divided into the negative control group and miR-128 overexpression group, and were transfected with miRNA-NC and miR-128 mim‐ ics, respectively. After 48 h, the cells were collected for follow-up experiments. Cell Scratch test and Transwell test were used to detect the migration and invasion abilities of cells in each group after transfection. Western blotting was used to de‐ tect ZEB1, E-cadherin, Fibronectin and Vimentin protein of cells in each group after transfection. Double luciferase re‐ porter gene experiment was used to verify the targeted regulatory relationship between miR-128 and ZEB1. Results Com‐ pared with hEEC, the expression of miR-128 in EC cell lines HEC-1A, Ishikawa and KLE decreased( all P<0. 05), and the expression of ZEB1 protein increased( P<0. 05) . After transfection, the expression of miR-128 increased significantly in Ishikawa cells( P<0. 05), the number of transmembrane cells and scratch healing rate decreased( all P<0. 05), the rel‐ ative expression levels of ZEB1, Fibronectin and Vimentin decreased( all P<0. 05), and the expression of E-cadherin protein increased( P<0. 05). The results of double luciferase reporter gene experiment showed that miR-128 could target and regulate ZEB1 gene( P<0. 05). Conclusions MiR-128 can inhibit the migration, invasion and EMT of Ishikawa cells. ZEB1 can promote the migration, invasion and EMT of Ishikawa cells. MiR-128 and ZEB1 have a targeted regulatory rela‐ tionship. [ABSTRACT FROM AUTHOR]

The acidizing fracturing flowback fluid with strong corrosion, high viscosity, high oil content and high suspended matter content was treated with "viscosity reduction, neutralization, de-stabilization and filtration". The results show that the optimal viscosity reduction conditions were as follows: pH was 4, the dosing quantity of H2O2 was 2 000 mg/L, the dosing quantity of Fe2+ was 500 mg/L, reaction time was 60 min and the optimal instability conditions were as follows: pH was 8, the dosing quantity of PAFC was 120 mg/L, the dosing quantity of PAM was 1. 2 mg/L. Under the optimized conditions and after comprehensive treatment, the oil content and SS content of the acidizing fracturing flowback fluid were 4. 4 mg/L and 1. 3 mg/L, the average corrosion rate was 0. 035 mm/a, the viscosity was 1. 4 mPa • s. The treated acidizing fracturing flowback fluid was mixed with formation water in any proportion, and the compatibility was good. All indicators meet the standard of re-injection. [ABSTRACT FROM AUTHOR]

A new inorganic-organic silicon-aluminum composite flocculant was synthesized by silane coupling agent γ-aminopropyltriethoxysilane and aluminum chloride under alkaline condition.The results show that the best synthetic conditions are as follows: the total aluminum concentration (AlT) is 0.2 mol/L, the molar ratio of Si/Al is 0.6 and the alkalization degree B value is 2.0.The pH value of the new inorganic-organic silicon-aluminum composite flocculant has positive correlation with B value and Si/Al molar ratio, and Zeta potential has negative correlation with B value and Si/Al molar ratio. The organic and inorganic components in the flocculant are bonded together by covalent bond, and the flocculant has a dense space network structure and excellent performance of adsorption and bridging. [ABSTRACT FROM AUTHOR]