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The rapid global population growth since the early 2000s has significantly increased the demand for agricultural products, leading to widespread pesticide use, particularly organophosphorus pesticides (OPPs). This extensive application poses severe environmental risks by contaminating air, soil, and water resources. To protect groundwater quality, it is crucial to understand the transport and fate of these pesticides in soil and sediment. This study investigates the effects of hydrochars and biochars derived from sugar beet shreds (SBS) and Miscanthus×giganteus (MIS) on the retardation and biodegradation of OPPs in alluvial Danube sandy soil. The research is novel in its approach, isolating native OPP-degrading bacteria from natural alluvial sandy soil, inoculating them onto chars, and reapplying these bioaugmented chars to the same soil to enhance biodegradation and reduce pesticide leaching. The amendment of chars with immobilized Bacillus megaterium BD5 significantly increased bacterial abundance and activity. Metabarcoding of the 16S rRNA gene revealed a dominance of Proteobacteria (48.0-84.8 %) and Firmicutes (8.3-35.6 %). Transport modeling showed retardation coefficients (R d ) for OPPs ranging from 10 to 350, with biodegradation rates varying between 0.05 % and 75 %, indicating a positive correlation between retardation and biodegradation. The detection of biodegradation byproducts, including derivatives of phosphin, pyridine, and pyrazole, in the column leachate confirmed that biodegradation had occurred. Additionally, principal component analysis (PCA) revealed positive correlations among retardation, biodegradation, specific surface area (SSA), aldehyde/ketone groups, and bacterial count. These findings demonstrate the potential of biochar and hydrochar amendments to enhance OPP immobilization in contaminated soils, thereby reducing their leaching into groundwater. This study offers a comprehensive approach to the remediation of pesticide-contaminated soils, advancing both our fundamental understanding and the practical applications of environmental remediation techniques., Competing Interests: Declaration of Competing Interest The authors would like to declare that they have no conflicts of interest associated with this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)