1. Cycles of satellite and transposon evolution in Arabidopsis centromeres
- Author
-
Wlodzimierz, Piotr, Rabanal, Fernando A, Burns, Robin, Naish, Matthew, Primetis, Elias, Scott, Alison, Mandáková, Terezie, Gorringe, Nicola, Tock, Andrew J, Holland, Daniel, Fritschi, Katrin, Habring, Anette, Lanz, Christa, Patel, Christie, Schlegel, Theresa, Collenberg, Maximilian, Mielke, Miriam, Nordborg, Magnus, Roux, Fabrice, Shirsekar, Gautam, Alonso-Blanco, Carlos, Lysak, Martin A, Novikova, Polina Y, Bousios, Alexandros, Weigel, Detlef, Henderson, Ian R, Rabanal, Fernando A [0000-0003-1538-9752], Naish, Matthew [0000-0002-8977-1295], Primetis, Elias [0000-0003-0502-2447], Gorringe, Nicola [0000-0003-1389-7630], Nordborg, Magnus [0000-0001-7178-9748], Alonso-Blanco, Carlos [0000-0002-4738-5556], Weigel, Detlef [0000-0002-2114-7963], Henderson, Ian R [0000-0001-5066-1489], Apollo - University of Cambridge Repository, University of Cambridge [UK] (CAM), Max-Planck-Institut fur Biologie = Max Planck Institute for Biology [Tübingen], Max-Planck-Gesellschaft, University of Sussex, Max Planck Society, Austrian Academy of Sciences (OeAW), Masaryk University [Brno] (MUNI), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Consejo Superior de Investigaciones Científicas (CSIC), This work was supported by BBSRC grants BB/S006842/1, BB/S020012/1 and BB/V003984/1, European Research Council Consolidator Award ERC-2015-CoG-681987, Marie Curie International Training Network MEICOM' and Human Frontier Science Program award RGP0025/2021 to I.R.H., EMBO long-term postdoctoral fellowship ALTF224-2022 to R.B., a Human Frontiers Science Program (HFSP) Long-Term Fellowship (LT000819/2018-L) to F.A.R., the Max Planck Society to D.W., an ERA-CAPS 1001G+ grant to M. Nordborg and D.W., Royal Society awards UF160222, URF\R\221024, RGF/R1/180006 and RGF/EA/201030 to A.B., European Research Council award ERC HOW2DOBLE 101041354 to P.Y.N., Czech Science Foundation grant no. 21-03909S to M.A.L., a BBSRC DTP Studentship to N.G., a Broodbank Fellowship to M. Naish, and grant PID2022-136893NB-I00 from the Ministerio de Ciencia e Innovacion of Spain/Agencia Estatal de Investigacion/10.13039/50110001103/FEDER, EU, to C.A.-B., and European Project: 951444,NHMRC::NHMRC Infrastructure Grants(1995)
- Subjects
Histones ,Evolution, Molecular ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Multidisciplinary ,[SDE]Environmental Sciences ,Centromere ,Arabidopsis ,DNA Transposable Elements ,Gene Conversion ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,DNA, Satellite ,[INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation ,Nucleosomes - Abstract
International audience; Centromeres are critical for cell division, loading CENH3 or CENPA histone variant nucleosomes, directing kinetochore formation and allowing chromosome segregation(1,2). Despite their conserved function, centromere size and structure are diverse across species. To understand this centromere paradox(3,4), it is necessary to know how centromeric diversity is generated and whether it reflects ancient trans-species variation or, instead, rapid post-speciation divergence. To address these questions, we assembled 346 centromeres from 66 Arabidopsis thaliana and 2 Arabidopsis lyrata accessions, which exhibited a remarkable degree of intra- and inter-species diversity. A. thaliana centromere repeat arrays are embedded in linkage blocks, despite ongoing internal satellite turnover, consistent with roles for unidirectional gene conversion or unequal crossover between sister chromatids in sequence diversification. Additionally, centrophilic ATHILA transposons have recently invaded the satellite arrays. To counter ATHILA invasion, chromosome-specific bursts of satellite homogenization generate higher-order repeats and purge transposons, in line with cycles of repeat evolution. Centromeric sequence changes are even more extreme in comparison between A. thaliana and A. lyrata. Together, our findings identify rapid cycles of transposon invasion and purging through satellite homogenization, which drive centromere evolution and ultimately contribute to speciation.
- Published
- 2023
- Full Text
- View/download PDF