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Cowpea ( Vigna unguiculata (L.) Walp.) is a diploid legume crop used for human consumption, feed for livestock, and cover crops. Earlier reports have shown that salinity has been a growing threat to cowpea cultivation. The objectives of this study were to conduct a genome-wide association study (GWAS) to identify SNP markers and to investigate candidate genes for salt tolerance in cowpea. A total of 331 cowpea genotypes were evaluated for salt tolerance by supplying a solution of 200 mM NaCl in our previous work. The cowpea panel was genotyped using a whole genome resequencing approach, generating 14,465,516 SNPs. Moreover, 5,884,299 SNPs were used after SNP filtering. GWAS was conducted on a total of 296 cowpea genotypes that have high-quality SNPs. BLINK was used for conducting GWAS. Results showed (1) a strong GWAS peak on an 890-bk region of chromosome 2 for leaf SPAD chlorophyll under salt stress in cowpea and harboring a significant cluster of nicotinamide adenine dinucleotide (NAD) dependent epimerase/dehydratase genes such as Vigun02g128900.1 , Vigun02g129000.1 , Vigun02g129100.1 , Vigun02g129200.1 , and Vigun02g129500.1 ; (2) two GWAS peaks associated with relative tolerance index for chlorophyll were identified on chromosomes 1 and 2. The peak on chromosome 1 was defined by a cluster of 10 significant SNPs mapped on a 5 kb region and was located in the vicinity of Vigun01g086000.1 , encoding for a GATA transcription factor. The GWAS peak on chromosome 2 was defined by a cluster of 53 significant SNPs and mapped on a 68 bk region of chromosome 2, and (3) the highest GWAS peak was identified on chromosome 3, and this locus was associated with leaf score injury. This peak was within the structure of a potassium channel gene ( Vigun03g144700.1 ). To the best of our knowledge, this is one the earliest reports on the salt tolerance study of cowpea using whole genome resequencing data.

The impact of salt stress is becoming more prevalent each year, largely due to the effects of climate change. Limited availability of salt-free water is rising concern for hydroponics lettuce production. Despite evidence supporting salt stress-induced quality losses and physiological changes, studies on romaine lettuce salt-stress tolerance are limited. This study examined the mechanism underlying the sodium chloride (NaCl) tolerance (0, 50, 100, and 150 mM) of lettuce on its growth and nutrition at late-rosette and early head-formation stages. Results revealed 76% fresh mass reduction under increased NaCl at both stages. The study also found unchanged carbon assimilation with reduced stomatal conductance under increased NaCl. Salt-stressed lettuce accumulated more boron and iron but had reduced phosphorus and calcium. Phenolics and sugars increased linearly under salt stress, suggesting that lettuce responds to increased oxidative stress at both stages. A positive association between salt treatment and sodium to potassium ion ratio indicated lettuce sensitivity to salt stress at both stages., Competing Interests: The authors declare that they have no conflict of interest., (Copyright: © 2023 Adhikari et al.)

Waterlogging causes various metabolic, physiological, and morphological changes in crops, resulting in yield loss of most legumes in rainfed and irrigated agriculture. However, research on cowpea genotypes using physiological and biochemical traits as a measure of tolerance to waterlogging stress is limited. We evaluated the impacts of 7 days of waterlogging (DOW) and 7 days of recovery (DOR) on the physiology and biochemistry of two cowpea (Vigna unguiculata (L.) Walp) genotypes (UCR 369 and EpicSelect.4) with contrasting waterlogging tolerance. Cowpea genotypes were grown in a controlled environment until the R2 stage and then subjected to 7 DOW. Later, the waterlogged plants were reoxygenated for an additional 7 DOR. Overall, cowpea genotypes had a contrasting response to waterlogging using different mechanisms. Compared to the control, the photosynthetic parameters of both cowpea genotypes were impaired under 7 DOW and could not recover at 7 DOR, with a larger decline in EpicSelect.4.7 DOW caused significant loss in the chlorophyll and carotenoid content of both genotypes. However, only waterlogged UCR 369 was not photo-inhibited and able to restore the levels of chlorophyll and carotenoids at 7 DOR. In addition, 7 DOW induced intense stress in UCR 369 with increased zeaxanthin, sucrose, and flavonoid content, while these metabolites were decreased in EpicSelect.4. On the other hand, glucose, fructose, and phenolic content were increased in EpicSelect.4 but decreased in UCR 369 at 7 DOR. In summary, compared to EpicSelect.4, UCR 369 restored their photosynthetic pigments and metabolites to the control levels at 7 DOR, indicating a likely tolerance to waterlogging stress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier Masson SAS.)

Due to climate change, the attainment of global food security is facing serious challenges in meeting the growing food demand. Abiotic stresses are the foremost limiting factors for agricultural productivity. However, not much information is available on the effect of multiple abiotic stresses on the morphological and biochemical aspects of kale and mustard. Therefore, an experiment was designed to study the effects of UV-B radiation, CO 2 concentration, and high temperature on the growth, yield, and biochemistry of two Brassica species, namely B. oleracea L. var. acephala Winterbor F1 (hybrid kale) and B. juncea var. Green wave O.G. (mustard greens), which were grown under optimal nutrients and soil moisture conditions in soil-plant-atmosphere-research (SPAR) units. Two levels of UV-B radiation (0 and 10 kJ m -2 d -1 ), two concentrations of CO 2 (420 and 720 ppm), and two different temperature treatments (25/17 °C and 35/27 °C) were imposed 12 days after sowing (DAS). Several morphological and biochemical parameters were measured at harvest (40 DAS) in both species. All the traits declined considerably under individual and multi-stress conditions in both species except under elevated CO 2 levels, which had a positive impact. Marketable fresh weight decreased by 64% and 58% in kale and mustard plants, respectively, growing under UV-B treatment. A slight increase in the chlorophyll content was observed in both species under the UV-B treatment alone and in combination with high temperature and elevated CO 2 . Understanding the impacts of high temperature, CO 2 , and UV-B radiation treatments on leafy vegetables, such as kale and mustard, can help to improve existing varieties to enhance resilience towards environmental stresses while simultaneously improving yield, morphology, and biochemistry in plants.

Waterlogging is an important environmental stress limiting the productivity of crops worldwide. Cowpea ( Vigna unguiculata L.) is particularly sensitive to waterlogging stress during the reproductive stage, with a consequent decline in pod formation and yield. However, little is known about the critical processes underlying cowpea's responses to waterlogging during the reproductive stage. Thus, we investigated the key parameters influencing carbon fixation, including stomatal conductance (g s ), intercellular CO 2 concentration, chlorophyll content, and chlorophyll fluorescence, of two cowpea genotypes with contrasting waterlogging tolerance. These closely related genotypes have starkly contrasting responses to waterlogging during and after 7 days of waterlogging stress (DOW). In the intolerant genotype ('EpicSelect.4'), waterlogging resulted in a gradual loss of pigment and decreased photosynthetic capacity as a consequent decline in shoot biomass. On the other hand, the waterlogging-tolerant genotype ('UCR 369') maintained CO 2 assimilation rate ( A ), stomatal conductance (g s ), biomass, and chlorophyll content until 5 DOW. Moreover, there was a highly specific downregulation of the mesophyll conductance (g m ), maximum rate of Rubisco (V cmax ), and photosynthetic electron transport rate (J max ) as non-stomatal limiting factors decreasing A in EpicSelect.4. Exposure of EpicSelect.4 to 2 DOW resulted in the loss of PSII photochemistry by downregulating the PSII quantum yield (F v /F m ), photochemical efficiency (Φ PSII ), and photochemical quenching (qP). In contrast, we found no substantial change in the photosynthesis and chlorophyll fluorescence of UCR 369 in the first 5 DOW. Instead, UCR 369 maintained biomass accumulation, chlorophyll content, and Rubisco activity, enabling the genotype to maintain nutrient absorption and photosynthesis during the early period of waterlogging. However, compared to the control, both cowpea genotypes could not fully recover their photosynthetic capacity after 7 DOW, with a more significant decline in EpicSelect.4. Overall, our findings suggest that the tolerant UCR 369 genotype maintains higher photosynthesis under waterlogging stress attributable to higher photochemical efficiency, Rubisco activity, and less stomatal restriction. After recovery, the incomplete recovery of A can be attributed to the reduced g s caused by severe waterlogging damage in both genotypes. Thus, promoting the rapid recovery of stomata from waterlogging stress may be crucial for the complete restoration of carbon fixation in cowpeas during the reproductive stage.

Waterlogging induces growth and developmental changes in sensitive crops such as cucumber ( Cucumis sativus L.) during early plant development. However, information on the physiological mechanisms underpinning the response of cucumber plants to waterlogging conditions is limited. Here, we investigated the effects of 10-day waterlogging stress on the morphology, photosynthesis, and chlorophyll fluorescence parameters in two cultivars of cucumber seedlings. Waterlogging stress hampered cultivars' growth, biomass accumulation, and photosynthetic capacity. Both cultivars also developed adventitious roots (ARs) after 10 days of waterlogging (DOW). We observed differential responses in the light- and carbon-dependent reactions of photosynthesis, with an increase in light-dependent reactions. At the same time, carbon assimilation was considerably inhibited by waterlogging. Specifically, the CO 2 assimilation rate ( A ) in leaves was significantly reduced and was caused by a corresponding decrease in stomatal conductance (g s ). The downregulation of the maximum rate of Rubisco efficiency (V cmax ) and the maximum rate of photosynthetic electron transport (J max ) were non-stomatal limiting factors contributing to A reduction. Exposure of cucumber to 10 DOW affected the PSII photochemistry by downregulating the PSII quantum yield (Φ PSII ). The redox state of the primary quinone acceptor in the lake model (1-qL), a measure of the regulatory balance of the light reactions, became more oxidized after 10 DOW, indicating enhanced electron sink capacity despite a reduced A . Overall, the results suggest that waterlogging induces alterations in the photochemical apparatus efficiency of cucumber. Thus, developing cultivars that resist inhibition of PSII photochemistry while maintaining carbon metabolism is a potential approach for increasing crops' tolerance to waterlogged environments., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Olorunwa, Adhikari, Brazel, Popescu, Popescu and Barickman.)

The majority of cowpea (Vigna unguiculata (L.) Walp.) produced in the U.S. is planted shortly after the summer rains and subsequently depends on rain or artificial irrigation. Therefore, excessive precipitation and poor soil drainage will cause cowpea plants to suffer temporary waterlogging, reducing the submerged tissue's oxygen level. Although cowpea is sensitive to waterlogging, excessive moisture can induce several morpho-physiological changes with adverse impacts on yield in its early stages of development. The current study subjected 30 cowpea genotypes to 10-days of waterlogging at the seedling stage under a controlled environment. The dynamic changes of 24 morpho-physiological parameters under waterlogging and optimal water conditions were analyzed to understand cowpea's response to waterlogging. Significant waterlogging treatment, cowpea genotypes, and their interactions (P < 0.001) were observed for most of the measured parameters. The results indicated that plant height (PH), leaf area (LA), fresh (FW) and dry weight (DW) of cowpea genotypes were significantly decreased under waterlogging compared to the control treatments. Similar results were obtained for net photosynthesis (P n ), stomatal conductance (g s ), intercellular CO 2 concentration (C i ), and transpiration rate (E). However, the water use efficiency (WUE) and adventitious roots (ARs) increased linearly under waterlogging conditions. Waterlogging also declined chlorophyll fluorescence parameters except non-photochemical quenching (qN), which increased with excess soil moisture. In addition, waterlogging tolerance coefficient (WTC) and multivariate analysis (MCA) methods were used to characterize cowpea genotypes for waterlogging tolerance. Accordingly, the cowpea genotype Dagupan Pangasinan, UCR 369, and Negro were classified as waterlogging tolerant, while EpicSelect.4 and ICARDA 140071, as the most waterlogging sensitive. The cowpea genotypes and morpho-physiological traits determined from this study may be useful for genetic engineering and breeding programs that integrate cowpea waterlogging tolerance., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)