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Based on the principle of virtual power, equilibrium conditions are established for the forces within a cross section of a tunnel top heading driven according the New Austrian Tunneling Method (NATM). External forces, namely impost actions and ground pressure distributions following a third-order polynomial, are analytically linked with internal forces, such as axial forces and bending moments, arising as integrals over the shell thickness, of circumferential normal stresses. The latter are related, via an aging viscoelastic shotcrete material model, to circumferential normal strains, as well as to radial and circumferential displacement components. This allows for analytical transformation of displacement measurement data collected at the crown and the footings of the shell segment, into ground pressure and impost action evolutions, together with all the associated force and stress quantities. For the Sieberg tunnel, driven in Miocene clay marl, our data-driven analytical mechanics model evidences virtually uniform ground pressure distributions, leading to a first rapidly increasing, and then mildly decreasing utilization degree of the shotcrete shell.

Imaged capillary isoelectric focusing (iCIEF) has emerged as an important technique for therapeutic monoclonal antibody (mAb) charge heterogeneity analysis in the biopharmaceutical context, providing imaged detection and quantitation by UV without a mobilization step. Besides quantitation, the characterization of separated charge variants ideally directly by online electrospray ionization-mass spectrometry (ESI-MS) is crucial to ensure product quality, safety, and efficacy. Straightforward direct iCIEF-MS coupling combining high separation efficiency and quantitative results of iCIEF with the characterization power of MS enables deep characterization of mAb charge variants. A short technical setup and optimized methodical parameters (30 nl/min mobilization rate, 2%-4% ampholyte concentration, 0.5-2 mg/ml sample concentration) allow successful mAb charge variant peak assignment from iCIEF to MS. Despite a loss of separation resolution during the transfer, separated intact mAb charge variants, including deamidation as well as major and minor glycoforms even from low abundant charge variants, could be characterized by online ESI-MS with high precision. The presented setup provides a large potential for mAb charge heterogeneity characterization in biopharmaceutical applications.