Search

Biological cells utilize membranes and liquid-like droplets, known as biomolecular condensates, to structure their interior. The interaction of droplets and membranes, despite being involved in several key biological processes, is so far little understood. Here, we present a first numerical method to simulate the continuum dynamics of droplets interacting with deformable membranes via wetting. The method combines the advantages of the phase-field method for multiphase flow simulation and the arbitrary Lagrangian-Eulerian method for an explicit description of the elastic surface. The model is thermodynamically consistent, coupling bulk hydrodynamics with capillary forces, as well as bending, tension, and stretching of a thin membrane. The method is validated by comparing simulations for single droplets to theoretical results of shape equations, and its capabilities are illustrated in two- and three-dimensional axisymmetric scenarios. [ABSTRACT FROM AUTHOR]

Abstract The diagnosis of neurodegenerative diseases (NDDs) remains challenging, and existing therapeutic approaches demonstrate little efficacy. NDD drug delivery can be achieved through the utilization of nanostructures, hence enabling multimodal NDD theranostics. Nevertheless, both biomembrane and non-biomembrane nanostructures possess intrinsic shortcomings that must be addressed by hybridization to create novel nanostructures with versatile applications in NDD theranostics. Hybrid nanostructures display improved biocompatibility, inherent targeting capabilities, intelligent responsiveness, and controlled drug release. This paper provides a concise overview of the latest developments in hybrid nanostructures for NDD theranostics and emphasizes various engineering methodologies for the integration of diverse nanostructures, including liposomes, exosomes, cell membranes, and non-biomembrane nanostructures such as polymers, metals, and hydrogels. The use of a combination technique can significantly augment the precision, intelligence, and efficacy of hybrid nanostructures, therefore functioning as a more robust theranostic approach for NDDs. This paper also addresses the issues that arise in the therapeutic translation of hybrid nanostructures and explores potential future prospects in this field.

The diagnosis of neurodegenerative diseases (NDDs) remains challenging, and existing therapeutic approaches demonstrate little efficacy. NDD drug delivery can be achieved through the utilization of nanostructures, hence enabling multimodal NDD theranostics. Nevertheless, both biomembrane and non-biomembrane nanostructures possess intrinsic shortcomings that must be addressed by hybridization to create novel nanostructures with versatile applications in NDD theranostics. Hybrid nanostructures display improved biocompatibility, inherent targeting capabilities, intelligent responsiveness, and controlled drug release. This paper provides a concise overview of the latest developments in hybrid nanostructures for NDD theranostics and emphasizes various engineering methodologies for the integration of diverse nanostructures, including liposomes, exosomes, cell membranes, and non-biomembrane nanostructures such as polymers, metals, and hydrogels. The use of a combination technique can significantly augment the precision, intelligence, and efficacy of hybrid nanostructures, therefore functioning as a more robust theranostic approach for NDDs. This paper also addresses the issues that arise in the therapeutic translation of hybrid nanostructures and explores potential future prospects in this field. [ABSTRACT FROM AUTHOR]

Cardiovascular diseases (CVDs) are one of the leading causes of death worldwide. Despite significant advances in current drug therapies, issues such as poor drug targeting and severe side effects persist. In recent years, nanomedicine has been extensively applied in the research and treatment of CVDs. Among these, biomembrane-modified biomimetic nanodrug delivery systems (BNDSs) have emerged as a research focus due to their unique biocompatibility and efficient drug delivery capabilities. By modifying with biological membranes, BNDSs can effectively reduce recognition and clearance by the immune system, enhance biocompatibility and circulation time in vivo, and improve drug targeting. This review first provides an overview of the classification and pathological mechanisms of CVDs, then systematically summarizes the research progress of BNDSs in the treatment of CVDs, discussing their design principles, functional characteristics, and clinical application potential. Finally, it highlights the issues and challenges faced in the clinical translation of BNDSs. [ABSTRACT FROM AUTHOR]

Saponins are a large group of compounds, produced mostly by plants as a side product of their metabolic activity. These compounds have attracted much attention over the years mostly because of their surface activity and antibacterial, anti-inflammatory and antifungal properties. On the other hand, most of the hitherto research has concerned the action of saponins against microbial cells as a whole. Therefore, knowing the possible interaction of saponins with biomembrane, we decided to check in-vitro the influence of saponin-rich extract of Saponaria officinalis on spheroplasts of two Candida sp. The obtained results show that 10 mg L− 1 of extract increased the permeability of spheroplasts up to 21.76% relative to that of the control sample. Moreover, the evaluation of surface potential has revealed a decrease by almost 10 mV relative to that of the untreated samples. Such results suggest its direct correlation to integration of saponins into the biomembrane structure. The obtained results have proved the antifungal potential of saponins and their ability of permeabilization of cells. This proves the high potential of saponins use as additives to antifungal pharmaceutics, which is expected to lead to improvement of their action or reduction of required dosage. [ABSTRACT FROM AUTHOR]

Using advanced nanotechnology membranes has opened up new possibilities in the field of biomedicine, particularly for controlled drug delivery and especially for topical use. Bacterial cellulose membranes (BCM), particularly, have gained prominence owing to their distinctive attributes, including remarkable water retention, safety, biodegradability, and tunable gas exchange. However, they are aqueous matrices and, for this reason, of limited capacity for incorporation of apolar compounds. Cubosomes are lipid nanoparticles composed of a surfactant bicontinuous reverse cubic phase, which, owing to their bicontinuous structure, can incorporate both polar and apolar compounds. Therefore, these particles present a promising avenue for encapsulating and releasing drugs and biomolecules due to their superior entrapment efficiency. In this study, we aim to extend earlier investigations using polymeric hydrogels for cubosome immobilization, now using BCMs, a more resilient biocompatible matrix. Phytantriol cubosome-loaded BCMs were prepared by three distinct protocols: ex situ incorporation into wet BCMs, ex situ incorporation by swelling of dry BCMs, and an in situ process with the growth of BCMs in a sterile medium already containing cubosomes. Our investigation revealed that these methodologies ensured that cubosomes remained integral, uniformly distributed, and thoroughly dispersed within the membrane, as confirmed using Small-Angle X-ray Scattering (SAXS) and high-resolution confocal microscopy. The effective incorporation and sustained release of diclofenac were validated across the different BCMs and compared with hyaluronic acid (HA) hydrogel in our previous studies. Furthermore, the resistance against cubosome leaching from the three BCM and HA hydrogel samples was quantitatively evaluated and contrasted. We hope that the outcomes from this research will pave the way for innovative use of this platform in the incorporation and controlled release of varied active agents, amplifying the already multifaceted applicability of BCMs.

Cancer remains a significant global health challenge, demanding innovative approaches to enhance the effectiveness and precision of therapies. In this pursuit, biomembrane-grafted dendrimer-polymeric conjugates have emerged as pioneering innovations within the field of cancer nanomedicine. These nanostructures represent a convergence of biology, materials science, and nanotechnology, offering a multifaceted platform with profound implications for cancer diagnosis and treatment. With precision drug delivery, they can transport therapeutics directly to cancer cells, minimizing systemic toxicity. Their utility extends to cancer imaging, enabling accurate visualization and monitoring of disease progression. Moreover, these conjugates facilitate personalized medicine by targeting specific genetic mutations, paving the way for tailored treatment approaches. Intriguingly, they hold promise in overcoming drug resistance by outsmarting efflux mechanisms and penetrating deep into tumor tissues. By combining multiple therapeutic agents, they enhance treatment efficacy through synergistic effects. This review delves into the intricacies of their design and the underlying mechanisms that govern their interactions within the tumor microenvironment. Furthermore, it highlights their potential to reshape cancer therapeutics by reducing side effects, inhibiting metastasis, and improving patient outcomes. As we navigate this innovative landscape, it becomes evident that biomembrane-grafted dendrimer-polymeric conjugates represent a powerful frontier in the battle against cancer, offering a beacon of hope for more effective and personalized treatment strategies. [ABSTRACT FROM AUTHOR]

The investigated starch biopolymer membrane was found to be a sustainable alternative to currently reported and used separators due to its properties, which were evaluated using physicochemical characterization. The molecular dynamics of the biomembrane were analyzed using low-field nuclear magnetic resonance (LF NMR) as well as Raman and infrared spectroscopy, which proved that the chemical composition of the obtained membrane did not degrade during microwave-assisted polymerization. Easily and cheaply prepared through microwave-assisted polymerization, the starch membrane was successfully used as a biodegradable membrane separating the positive and negative electrodes in electric double-layer capacitors (EDLCs). The obtained results for the electrochemical characterization via cyclic voltammetry (CV), galvanostatic charge with potential limitation (GCPL), and electrochemical impedance spectroscopy (EIS) show a capacitance of 30 F g−1 and a resistance of 2 Ohms; moreover, the longevity of the EDLC during electrochemical floating exceeded more than 200 h or a cyclic ability of 50,000 cycles. Furthermore, due to the flexibility of the membrane, it can be easily used in novel, flexible energy storage systems. This proves that this novel biomembrane can be a significant step toward ecologically friendly energy storage devices and could be considered a cheaper alternative to currently used materials, which cannot easily biodegrade over time in comparison to biopolymers. [ABSTRACT FROM AUTHOR]

The inspiring ideas of Professor Lester Packer (1929–2018) substantially enriched our understanding of biological systems. One of the most important contributions of Lester is the role of vitamin E in biological membranes. Lester started early in the 1970s with the development and use of a preparatory technique for electron microscopy of biological membranes, the "freeze fracture." This made it possible to detect inner and outer membranes of mitochondria as well as associated compounds in other biological organelles. Lester also considered the effect of tocols on entire animals and thereby initiated the field of exercise biology. An important finding was the loss of vitamin E and of muscle mitochondria after exhaustive exercise. In the 1990s, he and his group worked on the intermembrane exchange and membrane stabilization by tocols. They also determined the specific activities of various tocols including tocotrienols. In the later years they embarked on the role of vitamin E in redox signaling and gene expression, topics fundamental to our understanding of the role of vitamin E in membranes and in general. Lester, his group, and international guests tried to answer the still open question how vitamin E protects biomembranes. The numerous possibilities they offered will help to find a final solution. Lester always engaged himself at the forefront of science and in scientific exchange on meetings and in societies. Antioxid. Redox Signal. 39, 771–776. [ABSTRACT FROM AUTHOR]

Wen-Shang Liu,1,* Yu Liu,2,* Jie Gao,3,* Hao Zheng,4 Zheng-Mao Lu,4 Meng Li1 1Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai, People’s Republic of China; 2Department of Gastroenterology, Jinling Hospital, Medical School of Nanjing University, Nanjing, People’s Republic of China; 3Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai, People’s Republic of China; 4Department of General Surgery, Shanghai Changhai Hospital, Naval Medical University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Meng Li, Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai, People’s Republic of China, Tel +086-15000879978, Fax +086-021-23271699, Email lemonlives_dr@163.com Zheng-Mao Lu, Department of General Surgery, Shanghai Changhai Hospital, Naval Medical University, Shanghai, People’s Republic of China, Tel +086-13651688596, Fax +086-021-31161589, Email luzhengmao1982@163.comAbstract: Wound healing is a complex and dynamic process, and metabolic disturbances in the microenvironment of chronic wounds and the severe symptoms they cause remain major challenges to be addressed. The inherent properties of hydrogels make them promising wound dressings. In addition, biomembrane-based nanostructures and microstructures (such as liposomes, exosomes, membrane-coated nanostructures, bacteria and algae) have significant advantages in the promotion of wound healing, including special biological activities, flexible drug loading and targeting. Therefore, biomembrane-based nanostructure- and microstructure-loaded hydrogels can compensate for their respective disadvantages and combine the advantages of both to significantly promote chronic wound healing. In this review, we outline the loading strategies, mechanisms of action and applications of different types of biomembrane-based nanostructure- and microstructure-loaded hydrogels in chronic wound healing.Keywords: biomembrane, nanostructures, microstructures, hydrogels, chronic wound healing

The cell membrane undergoes various changes to modify its composition and mechanical properties to control the functioning of cellular mechanisms, in which cholesterol is highly involved. We have investigated the formation of cholesterol-rich nano-domains in pure 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) solid-supported lipid bilayer (SLB) at room temperature. The mechanical properties of the lipid bilayer in the presence of 0 to 40 mol% of cholesterol have been explored by using Peak-Force Quantitative Nano-Mechanical Atomic Force Microscopy. Cholesterol-rich domains with different mechanical strengths are distinctly observed at 5% cholesterol in SLB of DOPC-cholesterol. The cholesterol-rich domains with increased height are observed up to 25% cholesterol insertion within the bilayer. Further increase of cholesterol concentration leads to shrinkage of bilayer height and moderate rise of rigidity modulus. We believe that the reduction of bilayer height is due to the decoupling of the two leaflets within the membrane at high cholesterol concentration. [ABSTRACT FROM AUTHOR]

Using the EIS method (Electrochemical Impedance Spectroscopy), the interaction of the (Bilayer Lipid Membrane) BLM system with Ofloxacin in NaCl bath solutions was studied. The BLM system shows electrical properties and stability, which mainly depend on the concentration of the sodium chloride bath solution. The stability of the BLM system increased due to the increase in the concentration of NaCl. On the surface of BLM, a fixing impact has been created due to the cations. The Ofloxacin atoms get divided into the BLM stage and display the fluidization impact. The resistance of the membrane decreases with the concentration of Ofloxacin. To identify Ofloxacin in the arrangement, an impedimetric sensor was created. The level of Ofloxacin detected mainly depends on the sodium chloride concentration present in the bath solution. [ABSTRACT FROM AUTHOR]

Yougui pill is one of the representative prescriptions of "reinforcing Yang from Yin" theory (a basic theory of traditional Chinese medicine). Differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC), synchrotron small-angle X-ray scattering (SAXS), and Fourier transform infrared spectroscopy (FTIR) techniques were employed to explore the interaction of Yougui pill and its disassembled prescriptions (Yang-tonifying group and Yin-tonifying group) with liposome mimetic biomembrane. ITC experiments indicated that the whole Yougui pill has more favorable, stabilizing interaction with liposome mimetic biomembrane compared with the Yang-tonifying group and the Yin-tonifying group. DSC and SAXS experiments show that the whole Yougui pill has a stronger interaction with the head polar groups of the DPPC compared with the Yang-tonifying group and the Yin-tonifying group when containing the same concentration of indicator component. FTIR experiments show that the dehydration effect of the whole Yougui pill on the PO2− group of DPPC is greater than that of the Yang-tonifying group and greater than that of the Yin-tonifying group when containing the same concentration of indicator component. These conclusions may be related to the fact that the whole Yougui pill is more effective in treating kidney Yang deficiency syndrome than the Yang-tonifying group and than the Yin-tonifying group. [ABSTRACT FROM AUTHOR]

The bacterial cellulose membrane (CM) is a promising biomaterial due to its easy applicability and moist environment. Moreover, nanoscale silver compounds (AgNO 3) are synthesized and incorporated into CMs to provide these biomaterials with antimicrobial activity for wound healing. This study aimed to evaluate the cell viability of CM incorporated with nanoscale silver compounds, determine the minimum inhibitory concentration (MIC) for Escherichia coli and Staphylococcus aureus , and its use on in vivo skin lesions. Wistar rats were divided according to treatment: untreated, CM (cellulose membrane), and AgCM (CM incorporated with silver nanoparticles). The euthanasia was performed on the 2nd, 7th, 14th, and 21st days to assess inflammation (myeloperoxidase-neutrophils, N-acetylglucosaminidase-macrophage, IL-1β, IL-10), oxidative stress (NO-nitric oxide, DCF-H 2 O 2), oxidative damage (carbonyl: membrane's damage; sulfhydryl: membrane's integrity), antioxidants (superoxide dismutase; glutathione), angiogenesis, tissue formation (collagen, TGF-β1, smooth muscle α-actin, small decorin, and biglycan proteoglycans). The use of AgCM did not show toxicity, but antibacterial effect in vitro. Moreover, in vivo, AgCM provided balanced oxidative action, modulated the inflammatory profile due to the reduction of IL-1β level and increase in IL-10 level, in addition to increased angiogenesis and collagen formation. The results suggest the use of silver nanoparticles (AgCM) enhanced the CM properties by providing antibacterial properties, modulation the inflammatory phase, and consequently promotes the healing of skin lesions, which can be used clinically to treat injuries. • Silver nanoparticles (around 15-nm in size) were successfully incorporated into bacterial cellulose membranes. • Bacterial cellulose membrane (BCM) with silver nanoparticles: bacteriostatic property; no cytotoxicity for animal cells. • BCM with silver nanoparticles modulated the inflammatory process and stimulated angiogenesis and collagen formation. • Balanced oxidative stress and antioxidant markers by treating wounds with BCM incorporated with silver nanoparticles. [ABSTRACT FROM AUTHOR]

In the last decades, dendrimers have received attention in biomedicine that requires detailed study on the mechanism of their interaction with cell membranes. In this article, we report on the role of dendrimer structure in their interaction with liposomes. Here, the interactions between cationic pyridylphenylene dendrimers of the first, second, and third generations with mixed or completely charged pyridyl periphery (D16+, D215+, D229+, and D350+) with cholesterol-containing (CL/Chol/DOPC) anionic liposomes were investigated by microelectrophoresis, dynamic light scattering, fluorescence spectroscopy, and conductometry. It was found that the architecture of the dendrimer, namely the generation, the amount of charged pyridynium groups, the hydrophobic phenylene units, and the rigidity of the spatial structure, determined the special features of the dendrimer–liposome interactions. The binding of D350+ and D229+ with almost fully charged peripheries to liposomes was due to electrostatic forces: the dendrimer molecules could be removed from the liposomal surfaces by NaCl addition. D350+ and D229+ did not display a disruptive effect toward membranes, did not penetrate into the hydrophobic lipid bilayer, and were able to migrate between liposomes. For D215+, a dendrimer with a mixed periphery, hydrophobic interactions of phenylene units with the hydrocarbon tails of lipids were observed, along with electrostatic complexation with liposomes. As a result, defects were formed in the bilayer, which led to irreversible interactions with lipid membranes wherein there was no migration of D215+ between liposomes. A first-generation dendrimer, D16+, which was characterized by small size, a high degree of hydrophobicity, and a rigid structure, when interacting with liposomes caused significant destruction of liposomal membranes. Evidently, this interaction was irreversible: the addition of salt did not lead to the dissociation of the complex. [ABSTRACT FROM AUTHOR]

Passive permeation of cellular membranes is a key feature of many therapeutics. The relevance of passive permeability spans all biological systems as they all employ biomembranes for compartmentalization. A variety of computational techniques are currently utilized and under active development to facilitate the characterization of passive permeability. These methods include lipophilicity relations, molecular dynamics simulations, and machine learning, which vary in accuracy, complexity, and computational cost. This review briefly introduces the underlying theories, such as the prominent inhomogeneous solubility diffusion model, and covers a number of recent applications. Various machine-learning applications, which have demonstrated good potential for high-volume, data-driven permeability predictions, are also discussed. Due to the confluence of novel computational methods and next-generation exascale computers, we anticipate an exciting future for computationally driven permeability predictions.

The bark of Frangula alnus Mill (FAM), the so-called alder buckthorn, has been widely investigated for its medicinal properties, especially its laxative effects and the bioactive properties of the plant material extract. Still, there is no wider study devoted to its antibacterial properties. This is important in the context of its impact on probiotic gut bacteria. The aim of the research was to recognize the effect of FAM extract on bacterial cells, and to determine how the bioactive properties and composition of the extract are influenced by the type of solvent used for the extraction. To find the most suitable conditions for the FAM extraction, we used four solvent solutions with different polarities, including water, methanol, ethanol, and isopropanol. We assessed the quality and composition of the extracts with spectral analysis, using spectrophotometric (FTIR, UV-Vis) and chromatographic methods (GC-MS). Finally, we analyzed the extractant impact of the extracts on the selected bacterial cells. The results showed that the chemical diversity of the extracts increased with the increase in solvent polarity, in which the abundance of frangulin, the main bioactive compound in buckthorn bark, was confirmed. Pseudomonas fluorescens ATCC 17400 was particularly sensitive to the action of extracts, whereas other strains of the Pseudomonas genus showed practically no adverse effects. Ethanolic extracts had the strongest effect on most of the selected bacteria strains. We found that the probiotic Lactobacillus strain, which represents intestinal microflora, has no direct effect on probiotic microorganisms. The research shown FAM extracts can be safe for probiotic bacteria present in human gut microflora. Moreover, the study indicated that contact with the extracts may reduce the total permeability of the bacterial membranes. This opens up the possibility of using FAM extracts as a factor regulating transport into cells, which may be used to support the action of other bioactive substances. [ABSTRACT FROM AUTHOR]

Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions. Curcumin-loaded native protein-chitosan and succinylated protein-chitosan complexes, as well as native protein-chitosan and succinylated protein-chitosan hollow, induced leakage of the calcein encapsulated in the giant unilamellar vesicles. The leakage was more pronounced with hollow protein-chitosan complexes. However, curcumin-loaded native protein and curcumin-loaded succinylated protein nanoparticles induced calcein fluorescence quenching. Dynamic light scattering measurements showed that the interaction of curcumin-loaded native protein, curcumin-loaded succinylated protein, native protein-chitosan, and succinylated protein-chitosan complexes with the giant unilamellar vesicles caused a major reduction in the size of the lipid vesicles. Confocal and widefield fluorescence microscopy showed rupturing of the unilamellar vesicles after treatment with native pea protein-chitosan and succinylated pea protein-chitosan complexes. The nature of interaction between the curcumin-loaded protein nanoparticles and the biomembranes, at the bio-nano interface, is influenced by the encapsulated curcumin. Findings from this study showed that, as the protein plays a crucial role in stabilizing the bioactive compound from chemical and photodegradation, the encapsulated nutraceutical stabilizes the protein nanoparticle to reduce its interaction with biomembranes. [ABSTRACT FROM AUTHOR]

Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).

The bark of Frangula alnus Mill (FAM), the so-called alder buckthorn, has been widely investigated for its medicinal properties, especially its laxative effects and the bioactive properties of the plant material extract. Still, there is no wider study devoted to its antibacterial properties. This is important in the context of its impact on probiotic gut bacteria. The aim of the research was to recognize the effect of FAM extract on bacterial cells, and to determine how the bioactive properties and composition of the extract are influenced by the type of solvent used for the extraction. To find the most suitable conditions for the FAM extraction, we used four solvent solutions with different polarities, including water, methanol, ethanol, and isopropanol. We assessed the quality and composition of the extracts with spectral analysis, using spectrophotometric (FTIR, UV-Vis) and chromatographic methods (GC-MS). Finally, we analyzed the extractant impact of the extracts on the selected bacterial cells. The results showed that the chemical diversity of the extracts increased with the increase in solvent polarity, in which the abundance of frangulin, the main bioactive compound in buckthorn bark, was confirmed. Pseudomonas fluorescens ATCC 17400 was particularly sensitive to the action of extracts, whereas other strains of the Pseudomonas genus showed practically no adverse effects. Ethanolic extracts had the strongest effect on most of the selected bacteria strains. We found that the probiotic Lactobacillus strain, which represents intestinal microflora, has no direct effect on probiotic microorganisms. The research shown FAM extracts can be safe for probiotic bacteria present in human gut microflora. Moreover, the study indicated that contact with the extracts may reduce the total permeability of the bacterial membranes. This opens up the possibility of using FAM extracts as a factor regulating transport into cells, which may be used to support the action of other bioactive substances.

Nature has inspired the development of biomimetic membrane sensors in which the functionalities of biological molecules, such as proteins and lipids, are harnessed for sensing applications. This review provides an overview of the recent developments for biomembrane sensors compatible with either bulk or planar sensing applications, namely using lipid vesicles or supported lipid bilayers, respectively. We first describe the individual components required for these sensing platforms and the design principles that are considered when constructing them, and we segue into recent applications being implemented across multiple fields. Our goal for this review is to illustrate the versatility of nature's biomembrane toolbox and simultaneously highlight how biosensor platforms can be enhanced by harnessing it.

Artificial lipid bilayers have revolutionized biochemical and biophysical research by providing a versatile interface to study aspects of cell membranes and membrane-bound processes in a controlled environment. Artificial bilayers also play a central role in numerous biosensing applications, form the foundational interface for liposomal drug delivery, and provide a vital structure for the development of synthetic cells. But unlike the envelope in many living cells, artificial bilayers can be mechanically fragile. Here, we develop prototype scaffolds for artificial bilayers made from multiple chemically linked tiers of actin filaments that can be bonded to lipid headgroups. We call the interlinked and layered assembly a multiple minimal actin cortex (multi-MAC). Construction of multi-MACs has the potential to significantly increase the bilayer's resistance to applied stress while retaining many desirable physical and chemical properties that are characteristic of lipid bilayers. Furthermore, the linking chemistry of multi-MACs is generalizable and can be applied almost anywhere lipid bilayers are important. This work describes a filament-by-filament approach to multi-MAC assembly that produces distinct 2D and 3D architectures. The nature of the structure depends on a combination of the underlying chemical conditions. Using fluorescence imaging techniques in model planar bilayers, we explore how multi-MACs vary with electrostatic charge, assembly time, ionic strength, and type of chemical linker. We also assess how the presence of a multi-MAC alters the underlying lateral diffusion of lipids and investigate the ability of multi-MACs to withstand exposure to shear stress.

Toxic and carcinogenic metal (loid)s, such arsenic (As) and cadmium (Cd), found in contaminated paddy soils pose a serious danger to environmental sustainability. Their geochemical activities are complex, making it difficult to manage their contamination. Rice grown in Cd and As-polluted soils ends up in people's bellies, where it can cause cancer, anemia, and the deadly itai sickness. Solving this issue calls for research into eco-friendly and cost-effective remediation technology to lower rice's As and Cd levels. This research delves deeply into the origins of As and Cd in paddy soils, as well as their mobility, bioavailability, and uptake mechanisms by rice plants. It also examines the current methods and reactors used to lower As and Cd contamination in rice. Iron-modified biochar (Fe-BC) is a promising technology for reducing As and Cd toxicity in rice, improving soil health, and boosting rice's nutritional value. Biochar's physiochemical characteristics are enhanced by the addition of iron, making it a potent adsorbent for As and Cd ions. In conclusion, Fe-BC's biomembrane properties make them an attractive option for remediating As- and Cd-contaminated paddy soils. More efficient mitigation measures, including the use of biomembrane technology, can be developed when sustainable agriculture practices are combined with these technologies. [Display omitted] • Cadmium and arsenic have opposite geochemical behaviours in paddy soils. • Modifying biochar with iron increases its adsorption capacity for both cadmium and arsenic. • Iron-modified biochar decreased the bioavailability of metal (loid) and limited their uptake in rice plants. • Future goals include optimizing performance while reducing production costs. [ABSTRACT FROM AUTHOR]

Amidoxime compounds have been widely used in metal separation and recovery because of their excellent chelating properties to metal ions, especially to uranium (VI). In this study, N, N-bis (2-hydroxyethyl) malonamide was obtained from ethanolamine and dimethyl malonate, and used to prepare a two-dimensional network polymer, then the obtained polymer was immobilized in an environmentally friendly chitosan biomembrane, which enhanced its stability and hydrophobicity, meanwhile the amidoxime functionalization was achieved by oximation reaction of bromoacetonitrile, the application of the material further extends to uranium (VI) separation in solutions. Due to the synergistic action of amide group and amidoxime group, poly (ethanolamine-malonamide) based amidoxime biomembranes (PEA-AOM) showed extraordinary adsorption effect on uranium (VI), among which the saturation adsorption capacity of PEA-AOM-2 was 748.64 mg/g. PEA-AOM-2 also had good reusability (following five cycles of adsorption-desorption, the recovery rate maintained at 88%) and selectivity for uranium (VI), showing satisfactory results in competitive ion coexistence system and simulated seawater experiments. This study demonstrated that PEA-AOM-2 provided a new option for uranium (VI) separation in complex environment and low-concentration uranium background. [Display omitted] • A novel amidoxime functionalized polymer was immobilized in chitosan biomembranes. • PEA-AOM-2 showed remarkable adsorption capacity of 748.64 mg/g for uranium (VI). • PEA-AOM-2 demonstrated satisfactory selectivity for uranium (VI) up to 94.8% at pH 6.0. • PEA-AOM-2 had been fully verified in extraction uranium (VI) in simulated seawater. [ABSTRACT FROM AUTHOR]

In this paper we propose a novel numerical scheme for the Canham-Helfrich-Evans bending energy based on a three-field lifting procedure of the distributional shape operator to an auxiliary mean curvature field. Together with its energetic conjugate scalar stress field as Lagrange multiplier the resulting fourth order problem is circumvented and reduced to a mixed saddle point problem involving only second order differential operators. Further, we derive its analytical first variation (also called first shape derivative), which is valid for arbitrary polynomial order, and discuss how the arising shape derivatives can be computed automatically in the finite element software NGSolve. We finish the paper with several numerical simulations showing the pertinence of the proposed scheme and method. • Novel numerical scheme to discretize the Canham-Helfrich-Evans bending energy. • Lifting of distributional shape operator to regular function. • Derivation of distributional curvature in context of FEM. • Rigorous computation of first variation of the discretized bending energy. • Numerical minimization of energy by gradient-type algorithm using first variation. [ABSTRACT FROM AUTHOR]

In this article, the suitability of composite transdermal biomaterial for wound dressing applications is discussed. Bioactive, antioxidant Fucoidan and Chitosan biomaterials were doped into polyvinyl alcohol/β-tricalcium phosphate based polymeric hydrogels loaded with Resveratrol, which has theranostic properties, and biomembrane design with suitable cell regeneration properties was aimed. In accordance with this purpose, tissue profile analysis (TPA) was performed for the bioadhesion properties of composite polymeric biomembranes. Fourier Transform Infrared Spectrometry (FT-IR), Thermogravimetric Analysis (TGA) and Scanning Electron Microscopy (SEM-EDS) analyses were performed for morphological and structural analyses of biomembrane structures. In vitro Franz diffusion mathematical modelling of composite membrane structures, biocompatibility (MTT test) and in vivo rat tests were performed. TPA analysis of resveratrol loaded biomembrane scaffold design; compressibility; 13.4 ± 1.9(g.s), hardness; 16.8 ± 1(g), adhesiveness; −11 ± 2.0(g.s), elasticity; 0.61 ± 0.07, cohesiveness; 0.84 ± 0.04 were found. Proliferation of the membrane scaffold was 189.83 % at 24 h and 209.12 % at 72 h. In the in vivo rat test; at the end of 28th day, it was found that biomembrane_3 provided 98.75 ± 0.12 % wound shrinkage. The shelf-life of RES in the transdermal membrane scaffold, which was determined as Zero order according to Fick's law in in vitro Franz diffusion mathematical modelling, was found to be approximately 35 days by Minitab statistical analysis. The importance of this study is that the innovative and novel transdermal biomaterial supports tissue cell regeneration and cell proliferation in theranostic applications as a wound dressing. [Display omitted] [ABSTRACT FROM AUTHOR]

The alginate-based multi-crosslinked biomembranes (ABMCBs) were prepared mainly with sodium alginate as matrix and self-made functionalized organosilane containing different groups as additive. The properties of ABMCBs with various additive loading were investigated as proton exchange membranes (PEMs). The results showed that higher water absorption and lower swelling were obtained simultaneously with increasing additive loading, which is very beneficial to the use of PEMs. The ABMCB-4 containing 40 wt% additive exhibited the optimal selectivity and maximum power density, which were obviously higher than that of commercial Nafion@ 117. Furthermore, ABMCB-4 possessed excellent mechanical property, methanol barrier and stability, indicating its potential adaptability as PEM for direct methanol fuel cell application. [Display omitted] • The alginate-based multi-crosslinked biomembranes (ABMCBs) were prepared. • ABMCBs possessed good physicochemical stability. • More water uptake and less swelling were obtained simultaneously in ABMCBs. • ABMCBs showed significantly more excellent methanol barrier and selectivity than Nafion@ 117. • Compared with Nafion@ 117, ABMCB-4 exhibited better DMFC performance. [ABSTRACT FROM AUTHOR]

Circulating exosomes (crEVs) have attracted increasing interest in non-invasive cancer diagnosis and monitoring of treatment response. In this paper, cancer cell membrane functionalized AuNPs (CCM@AuNPs) containing overexpressed Tenascin-C (TNC) were synthesized as a biomimetic probe for crEVs. crEVs containing fibronectin 1 (FN1) could be recognized through the specific interaction between FN1 and TNC. CCM@AuNPs were immobilized on the gold chip surface to decrease nonspecific interactions and enhance the surface plasmon resonance signal to ensure the sensor's accuracy and specificity. The ultra-sensitive crEVs detection was achieved through CCM@AuNPs with a low detection limit of 18.1 particles/mL−1 and the linear range of 3 × 104∼3 × 107 particles mL−1. Owing to its simple and direct detection of crEVs, this biomimetic strategy for detecting crEVs offers new possibilities for clinical non-invasive cancer diagnosis. • Cancer cell-functionalized AuNPs (CCM@AuNPs) are successfully prepared. • A Surface plasmon resonance (SPR) sensing platform was designed based on CCM@AuNPs to achieve the sensitive detection of crEVs. • The ultra-sensitive crEVs detection was achieved through CCM@AuNPs with a low detection limit of 18.1 particles/mL−1. [ABSTRACT FROM AUTHOR]

[Display omitted] • Castes and ethotypes of Apis mellifera form qualitatively distinct classes of membrane lipid composition. • Cardiolipins are the lipid class most strongly differentiating castes and ethotypes. • Sexuals and cell-heater bees are characterized by highly-saturated, short-chained cardiolipins. • Forager bees show an inverse pattern. • Lipid composition can be experimentally influenced through fatty acids and brood rearing temperature. Honeybees of the same colony combine a near-homogeneous genetic background with a high level of phenotypic plasticity, making them ideal models for functional lipidomics. The only external lipid source of the colony is pollen, a diet rich in polyunsaturated fatty acids (PUFA). It has been suggested that differences in exposure to pollen-derived PUFA could partly explain differences in longevity between honeybee castes. We here investigated whether the membrane composition of honeybees plays roles in the physiological adaptation to tasks of individuals within the colony. Membranes of cell heaters, a group of workers producing heat from their flight muscles to uphold brood nest temperature, were compared to those of different types of non-heaters. We found that the lipidomic profiles of these groups fall into clearly different "lipotypes", characterized by chain length and saturation of phospholipid-bound fatty acyl residues. The nutritional exposure to PUFA during early adult life and pupal development at the lower edge of the natural range of brood nest temperature both suppressed the expression of the cell heater-"lipotype". Because cardiolipins (CL) are the lipid class most clearly differentiating honeybee phenotypes, and CL plays central roles in mitochondrial function, dysfunction and aging, our findings could help to understand these processes in other animals and humans. Taken together, the lipidome analysis of different life stages of workers, fertile queens, and drones lead to the hypothesis that honeybee "lipotypes" might represent adaptations to different energetic profiles and the likelihood of exposure to low temperatures. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the potential of 1-alkyl-1-methylpiperidinium bromides as fungicides and evaluate their impact on the human respiratory system when spread in the atmosphere. We investigated the behavior of membrane lipids and model membranes in the presence of a series of amphiphilic 1-alkyl-1-methylpiperidinium bromides ([MePipC n ][Br]), differing in the alkyl chain length (n = 4 − 18). The experiments were performed with the Langmuir monolayer technique using 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) and ergosterol (ERG)–the main components of lung surfactant and fungal plasma membrane, respectively and their mixtures with phospholipids and sterols. The mixtures were chosen as the representatives of target and non−target organisms. The surface pressure−area isotherms were obtained by compressing monolayers in the presence of [MePipC n ][Br] in the subphase. The results were analyzed in terms of area expansion/contraction and compressibility. The surface activity of the studied organic salts was also studied. In addition, the monolayers were deposited on a solid surface and their topography was investigated using atomic force microscopy. This research implies that the studied compounds may destabilize efficiently the fungal plasma membrane. At the same time we demonstrated the significant impact of 1-alkyl-1-methylpiperidinium bromides on the lung surfactant layer. The interaction between [MePipCn][Br] and model membranes depends on the concentration and alkyl chain length of organic salt. The key role of contact time has been also revealed. The results may be helpful in the reasonable development of new agrochemical products aiming at the treatment of fungal infections in plants. In addition, our study indicates the significance of proper safety management while spreading the fungicides in the environment. [Display omitted] • 1-Alkyl-1-methylpiperidinium bromides show activity against fungal species. • The compounds may potentially affect pulmonary surfactant layer. • 1-Alkyl-1-methylpiperidinium are promising compounds as antifungal agents. [ABSTRACT FROM AUTHOR]

The electrostatic interactions between cationic poly(amidoamine) (PAMAM) dendrimers of different generations, G3, G4, and G6, with net anionic model biomembranes have been predicted by adopting an analytical model based on two dissimilar soft spheres. The influence of bilayer surface charge density, ionic strength, pH, temperature, membrane softness (modeled as changes in bilayer thickness), and dendrimer generation on the attractive interaction was investigated. The attraction was found to decrease with increasing salt concentration, dendrimer charge, and thickness (or softness) of the membrane. On the other hand, the attraction increased with the surface charge density of the membrane, and the size of dendrimer generation. In fact, the attraction was found to be much larger for large generations, like G6 dendrimer that have a higher charge, than it is with small ones like G3 and G4 dendrimers. These results have implications for the use of PAMAM dendrimers as potential gene transfection vectors. [ABSTRACT FROM AUTHOR]

Organ-on-a-chip (OOC) systems are engineered nanobiosystems to mimic the physiochemical environment of a specific organ in the body. Among various components of OOC systems, biomimetic membranes have been regarded as one of the most important key components to develop controllable biomimetic bioanalysis systems. Here, we review the preparation and characterization of biomimetic membranes in comparison with the features of the extracellular matrix. After that, we review and discuss the latest applications of engineered biomimetic membranes to fabricate various organs on a chip, such as liver, kidney, intestine, lung, skin, heart, vasculature and blood vessels, brain, and multiorgans with perspectives for further biomedical applications.

Optogenetics in the conventional sense, i.e. the use of engineered proteins that gain their light sensitivity from naturally abundant chromophores, represents an exciting means to trigger and control biological activity by light. As an alternate approach, photopharmacology controls biological activity with the help of synthetic photoswitches. Here, we used an azobenzene-derived lipid analogue to optically activate the transmembrane mechanosensitive channel MscL which responds to changes in the lateral pressure of the lipid bilayer. In this work, MscL has been reconstituted in nanodiscs, which provide a native-like environment to the protein and a physical constraint to membrane expansion. We characterized this photomechanical system by FTIR spectroscopy and assigned the vibrational bands of the light-induced FTIR difference spectra of the trans and cis states of the azobenzene photolipid by DFT calculations. Differences in the amide I range indicated reversible conformational changes in MscL as a direct consequence of light switching. With the mediation of nanodiscs, we inserted the transmembrane protein in a free standing photoswitchable lipid bilayer, where electrophysiological recordings confirmed that the ion channel could be set to one of its sub-conducting states upon light illumination. In conclusion, a novel approach is presented to photoactivate and control cellular processes as complex and intricate as gravitropism and turgor sensing in plants, contractility of the heart, as well as sensing pain, hearing, and touch in animals., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Crea, Vorkas, Redlich, Cruz, Shi, Trauner, Lange, Schlesinger and Heberle.)