1. Towards chloroplastic nanofactories: formation of proteinaceous scaffolds for metabolic engineering.
- Author
-
Dwyer, Matthew E., Froehlich, John E., Raba, Daniel A., Borrusch, Melissa, Danhof, Linda, Sharma, Naveen, Young, Eric J., Brandizzi, Federica, Benning, Christoph, and Kerfeld, Cheryl A.
- Subjects
- *
BIOENGINEERING , *MOLECULAR biology , *SMALL molecules , *ESCHERICHIA coli , *PEPTIDES - Abstract
This article explores the use of specialized bacterial microcompartments (BMCs) as a platform for metabolic engineering in plants. The researchers conducted experiments using Arabidopsis thaliana chloroplasts and introduced genes encoding synthetic BMC shell proteins. The study successfully demonstrated the potential for assembling nanofactories within the chloroplasts to enhance metabolic processes in plants. The researchers were able to construct and purify wiffle ball shells in Arabidopsis chloroplasts, showcasing the potential of this synthetic scaffold system. The research was supported by the Department of Energy and MSU AgBioResearch. [Extracted from the article]
- Published
- 2024
- Full Text
- View/download PDF