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This work presents observations of symmetry breakages in the intensity distributions of near-zone-axis convergent-beam electron diffraction (CBED) patterns that can only be explained by the symmetry of the specimen and not the symmetry of the unit cell describing the atomic structure of the material. The specimen is an aluminium-copper-tin alloy containing voids many tens of nanometres in size within continuous single crystals of the aluminium host matrix. Several CBED patterns where the incident beam enters and exits parallel void facets without the incident beam being perpendicular to these facets are examined. The symmetries in their intensity distributions are explained by the specimen morphology alone using a geometric argument based on the multislice theory. This work shows that it is possible to deduce nanoscale morphological information about the specimen in the direction of the electron beam - the elusive third dimension in transmission electron microscopy - from the inspection of CBED patterns., (© Xiaofen Tan et al. 2024.)

Despite the clinical benefits that chemotherapeutics has had on the treatment of breast cancer, drug resistance remains one of the main obstacles to curative cancer therapy. Nanomedicines allow therapeutics to be more targeted and effective, resulting in enhanced treatment success, reduced side effects, and the possibility of minimising drug resistance by the co-delivery of therapeutic agents. Porous silicon nanoparticles (pSiNPs) have been established as efficient vectors for drug delivery. Their high surface area makes them an ideal carrier for the administration of multiple therapeutics, providing the means to apply multiple attacks to the tumour. Moreover, immobilising targeting ligands on the pSiNP surface helps direct them selectively to cancer cells, thereby reducing harm to normal tissues. Here, we engineered breast cancer-targeted pSiNPs co-loaded with an anticancer drug and gold nanoclusters (AuNCs). AuNCs have the capacity to induce hyperthermia when exposed to a radiofrequency field. Using monolayer and 3D cell cultures, we demonstrate that the cell-killing efficacy of combined hyperthermia and chemotherapy via targeted pSiNPs is 1.5-fold higher than applying monotherapy and 3.5-fold higher compared to using a nontargeted system with combined therapeutics. The results not only demonstrate targeted pSiNPs as a successful nanocarrier for combination therapy but also confirm it as a versatile platform with the potential to be used for personalised medicine.

Many new circular economy technologies are gaining momentum, yet research on the complexity of adoption decisions driven by uncertainties, both at technology and ecosystem level, is lacking. In the present study, an agent-based model was developed to study factors that influence the adoption of emerging circular technologies. The case of the waste treatment industry was chosen, specifically its (non-) adoption of the so-called "Volatile Fatty Acid Platform", a circular economy technology that facilitates both the valorization of organic waste into high-end products as well as their sale on global markets. Model results show adoption rates under 60% due to effects of subsidies, market growth, technological uncertainty and social pressure. Furthermore, the conditions were revealed under which certain parameters have the most effect. An agent-based model enabled use of a systemic approach to reveal the mechanisms of circular emerging technology innovation that are most relevant for researchers and waste treatment stakeholders., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Author(s).)

Inspired by nature, green chemistry uses various biomolecules, such as proteins, as reducing agents to synthesize metallic nanostructures. This methodology provides an alternative route to conventional harsh synthetic processes, which include polluting chemicals. Tuning the resulting nanostructure properties, such as their size and shape, is challenging as the exact mechanism involved in their formation is still not well understood. This work reports a well-controlled method to program gold nanostructures' shape, size, and aggregation state using only one protein type, mucin, as a reduction and capping material in a one-pot bio-assisted reaction. Using mucin as a gold reduction template while varying its tertiary structure via the pH of the synthesis, we demonstrate that spherical, coral-shaped, and hexagonal gold crystals can be obtained and that the size can be tuned over three orders of magnitude. This is achieved by leveraging the protein's intrinsic reducing properties and pH-induced conformational changes. The systematic study of the reaction kinetics and growth steps developed here provides an understanding of the mechanism behind this phenomenon. We further show that the prepared gold nanostructures exhibit tunable photothermal properties that can be optimized for various hyperthermia-induced antibacterial applications., (© 2022. The Author(s).)

Nickel oxide exhibits almost the highest theoretical specific capacitance ( C s ), which includes contributions from non-faradaic double layer charging and faradaic OH - adsorption. However, the realistic and tangible C s is due to the faradaic process, which can be influenced by chemical ( i.e. stoichiometry) and structural ( i.e. grain size) changes. Hence, it is necessary to investigate the interrelationships among chemical and structural features and charge storage capacity. Here, a non-stoichiometric nickel oxide (Ni x O) containing Ni 2+ and Ni 3+ was synthesized by a sol-gel method at 620, 720 and 920 °C using Ni(NO 3 ) 2 ·6H 2 O and citric acid. The grain size as estimated from X-ray diffraction increases from 55 to 194 nm with increase in the synthesis temperature. The stoichiometry measured through Ni 2+ (or Ni 3+ ) fraction from X-ray photoelectron spectroscopy also increases from 70.3 to 99.2 atom% with synthesis temperature. The C s due to faradaic OH - adsorption was estimated from cyclic voltammetry in 2 M KOH within -0.05 to +0.60 V vs. Hg/Hg 2 Cl 2 /KCl (sat. in water). This C s increases from 7.5 to 92.4 F g -1 with a decrease in the grain size and stoichiometry (increase in Ni 3+ ) due to possibly the increased conductivity and NiOOH formation through OH - adsorption. The deviation from stoichiometry at lower grain size mainly stems from nickel vacancy accommodation, according to the thermodynamic model proposed here. The interrelationships among stoichiometry, grain size and the specific capacitance of nickel oxide are investigated., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Confirmed diagnosis of alveolar echinococcosis (AE) is based on pathological criteria and molecular evidence. This parasite-borne disease, caused by the cestode Echinococcus multilocularis, sparingly involves humans as a dead-end host. In humans, the parasite mainly colonizes the liver but can colonize any organ and cause atypical forms, often difficult to characterize clinically. Moreover, molecular methods may be suitable to make the diagnosis of AE in cases of atypical forms, extra-hepatic localizations, or immunosuppressed patients. The aim of this study was to determine the most relevant published PCR techniques, for diagnosis of AE in patients and adopt the best strategy for molecular diagnosis depending on the nature of the tested sample. In this study, we evaluated nine end-point PCR assays and one real-time PCR assay (qPCR), targeting mitochondrial genes, using a total of 89 frozen or formalin-fixed paraffin-embedded (FFPE) samples from either 48 AE or 9 cystic echinococcosis patients. Targeted fragment-genes ranged from 84 to 529 bp. Six PCR assays were able to amplify the DNA of 100% of the frozen AE-samples and for one PCR, 69.8% of the FFPE AE-samples. The 16S rrnL PCR (84 bp) was positive in PCR for 77% of the AE samples and in qPCR for 86.5%. The sensitivity of the PCR assays was higher for fresh samples and FFPE samples stored for less than 5 years. The qPCR assay further increased sensitivity for the tested samples, confirming the need for the development of an Echinococcus spp. qPCR to improve the molecular diagnosis of echinococcoses., (© J. Knapp et al., published by EDP Sciences, 2022.)