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Your search keyword '"Buhler, Stéphane"' showing total 8 results
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8 results on '"Buhler, Stéphane"'

2. The most frequent HLA alleles around the world: A fundamental synopsis

Author

Di, Da, Dominguez, Enrique Alanis, Gerbault, Pascale, Faye, Ndeye Khady, Almawi, Wassim, Andreani, Marco, Arrieta-Bolanos, Esteban, Augusto, Danillo G., Buhler, Stéphane, de Groot, Natasja G., Díaz-Peña, Roberto, D'Silva, Selma, Dubois, Valérie, ElGhazali, Gehad, Gorodezky, Clara, Grubić, Zorana, Hajeer, Ali, Hollenbach, Jill, Issler, Hellen C., Ivanova, Milena, Jawdat, Dunia, Kanga, Uma, Kirijas, Meri, Lemieux, William, Loginova, Maria, Mahfoudh, Nadia, Mehra, Narinder, Mouron, Delphine, Petřek, Martin, Petzl-Erler, Maria Luiza, Richard, Lucie, Roelen, Dave L., Sacchi, Nicoletta, Sharma, Akanksha, Singh, Bisu, Singh, Meenakshi, Singh, Yoginder, Tarassi, Katerina, Vidan-Jeras, Blanka, Villard, Jean, Sanchez-Mazas, Alicia, and Nunes, José Manuel

Published
2024
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5. High resolution HLA genotyping with third generation sequencing technology—A multicentre study.

Author

Buhler, Stéphane, Nørgaard, Maja, Steffensen, Rudi, Kløve‐Mogensen, Kirstine, Møller, Bjarne Kuno, Grossmann, Rebecca, Ferrari‐Lacraz, Sylvie, and Lehmann, Claudia

Subjects
*NUCLEOTIDE sequencing, *STEM cell transplantation, *QUALITY control, *EPIGENETICS, *GENOMICS, *DEAD
Abstract

Molecular HLA typing techniques are currently undergoing a rapid evolution. While real‐time PCR is established as the standard method in tissue typing laboratories regarding allocation of solid organs, next generation sequencing (NGS) for high‐resolution HLA typing is becoming indispensable but is not yet suitable for deceased donors. By contrast, high‐resolution typing is essential for stem cell transplantation and is increasingly required for questions relating to various disease associations. In this multicentre clinical study, the TGS technique using nanopore sequencing is investigated applying NanoTYPE™ kit and NanoTYPER™ software (Omixon Biocomputing Ltd., Budapest, Hungary) regarding the concordance of the results with NGS and its practicability in diagnostic laboratories. The results of 381 samples show a concordance of 99.58% for 11 HLA loci, HLA‐A, ‐B, ‐C, ‐DRB1, ‐DRB3, ‐DRB4, ‐DRB5, ‐DQA1, ‐DQB1, ‐DPA1 and ‐DPB1. The quality control (QC) data shows a very high quality of the sequencing performed in each laboratory, 34,926 (97.15%) QC values were returned as 'passed', 862 (2.4%) as 'inspect' and 162 (0.45%) as 'failed'. We show that an 'inspect' or 'failed' QC warning does not automatically lead to incorrect HLA typing. The advantages of nanopore sequencing are speed, flexibility, reusability of the flow cells and easy implementation in the laboratory. There are challenges, such as exon coverage and the handling of large amounts of data. Finally, nanopore sequencing presents potential for applications in basic research within the field of epigenetics and genomics and holds significance for clinical concerns. [ABSTRACT FROM AUTHOR]

Published
2024
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