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Objective To analyze the spatial distribution characteristics, efficiency, and influencing factors of pension institutions in Dongguan, so as to provide reference for optimizing the resource allocation of pension institutions in Dongguan. Methods The resource allocation data of pension institutions in Dongguan were obtained by questionnaire survey, and the spatial distribution characteristics and efficiency of pension institutions were analyzed and evaluated based on GIS and three-stage DEA model. Results There was a good spatial coupling between the resources of pension institutions in Dongguan and the elderly population over 60 years old; the lowest occupancy rate was 12.50%, the highest was 100.005%, and the phenomenon of "difficulty in finding a bed" and "half of the beds were vacant" coexisted. In the third stage, the average comprehensive efficiency decreased from 0.679 to 0.635, and environmental factors had a great influence on the efficiency of resource allocation in Dongguan. There were great differences in the efficiency of pension institutions in different areas. The comprehensive efficiency of urban areas was 0.784, which exceeded the average level of the whole city (0.635). The comprehensive efficiency of the eastern industrial park was only 0.439, which did not reach the average level of the whole city. The per capita regional GDP and the level of aging had a negative impact on efficiency. Conclusion It is suggested to rationally allocate the resources of pension institutions, optimize regional development planning, promote the upgrading of institutional pension services, improve the efficiency of resource allocation, and provide improved reference scheme for improving the service level of pension institutions in Dongguan. [ABSTRACT FROM AUTHOR]

Aim: To observe the effect of low oxygen concentration on the neural retina in human induced pluripotent stem cell (hiPSC)-derived retinal organoids (ROs)., Methods: The hiPSC and a three-dimensional culture method were used for the experiments. Generated embryoid bodies (EBs) were randomly and equally divided into hypoxic and normoxic groups. Photographs of the EBs were taken on days 38, 45, and 52, and the corresponding volume of EBs was calculated. Simultaneously, samples were collected at these three timepoints, followed by fixation, sectioning, and immunofluorescence., Results: The proportion of Ki67-positive proliferating cells increased steadily on day 38; this proliferation-promoting effect tended to increase tissue density rather than tissue volume. On days 45 and 52, the two groups had relatively similar ratios of Ki67-positive cells. Further immunofluorescence analysis showed that the ratio of SOX2-positive cells significantly increased within the neural retina on day 52 ( P <0.05). In contrast, the percentage of PAX6- and CHX10-positive cells significantly decreased following hypoxia treatment at all three timepoints ( P <0.01), except for CHX10 at day 45 ( P >0.05). Moreover, the proportion of PAX6 - /TUJ1 + cells within the neural retinas increased considerably ( P <0.01, <0.05, <0.05 respectively)., Conclusion: Low oxygen promotes stemness and proliferation of neural retinas, suggesting that hypoxic conditions can enlarge the retinal progenitor cell pool in hiPSC-derived ROs., (International Journal of Ophthalmology Press.)