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Your search keyword '"Dagmar Klein"' showing total 4 results
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4 results on '"Dagmar Klein"'

1. Contributors

Author

Ahmed Safwat Abouhashem, Aamir Ahmad, Shazia Ahmad, Saira R. Ali, Tyler Anderson, Daniele Avitabile, Asha Balakrishnan, Mumtaz Yaseen Balkhi, Bin Bao, Nasma Bastaki, Christophe Beclin, Soumaya Ben-Aicha, Andreas Bosio, Emily Bruch, George A. Calin, Yang Cao, Maurizio C. Capogrossi, Andrea Caporali, Derryn Xin Hui Chan, Yuk Cheung Chan, Pavithra L. Chavali, Sreenivas Chavali, Alex F. Chen, Xiaona Chen, Charles Cook, Harold Cremer, Catherine Czeisler, Duaa Dakhlallah, Amitava Das, Anne M. Delany, Dasa Dolezalova, Juan Domínguez-Bendala, Manar A. EI Naggar, Costanza Emanueli, Michael Ezzie, Sara T. Fathallah, Tiziana Franceschetti, Roberto Gambari, Subhadip Ghatak, Jonathan M. Gleadle, Le Luo Guan, Denis C. Guttridge, Patrick Edwin Gygli, Khawaja H. Haider, Aleš Hampl, Sen Han, Martin C. Harmsen, Yoshinori Hasegawa, Sara A. Hashish, Eric Hesse, John D. Houlé, Kazuki Inoue, Jared Jagdeo, Imran Khan, Mahmood Khan, Shirin Elizabeth Khorsandi, Dagmar Klein, Dejuan Kong, Guido Krenning, Praveen Kusumanchi, Yiwei Li, Zhigang Li, Suthat Liangpunsakul, Kenneth W. Liechty, Amanda Louiselle, Leina Lu, Alessandra Magenta, Nilusha Malmuthuge, Andrew Mamalis, Clay B. Marsh, Selina Möbus, Ganesh Mohan, Peter J. Mohler, Leni Moldovan, Paloma del C. Monroig, Marek Mraz, S. Patrick Nana-Sinkam, Colby R. Neumann, Stephen Niemiec, José Javier Otero, Durba Pal, Ricardo L. Pastori, Melissa G. Piper, Giulio Pompilio, Mirza Muhammed Fahd Qadir, Srinivas Ramsamy, Darling Rojas-Canales, Alessandra Rossini, Sashwati Roy, Yashika Rustagi, Alaa A. Salama, Mohamed Salama, Prabha Sampath, Fazlul H. Sarkar, Mitsuo Sato, Chandan K. Sen, David S. Shames, Amar Deep Sharma, Anjali Kumari Singh, Kanhaiya Singh, Mithun Sinha, Prashant Srivastava, Hao Sun, Yeqing Sun, Hidetoshi Tahara, Hanna Taipaleenmäki, Joanne Trgovich, Elise J. Tucker, Huating Wang, Jie-Mei Wang, Lijun Wang, Yijie Wang, Brandon Watson, Dan Xu, Junwang Xu, Yi Xuan, Dakai Yang, Zhihong Yang, Nouran Yonis, Marina E. Zambrotta, Carlos Zgheib, Ting Zhang, Baohong Zhao, Yu Zhao, and Liang Zhou

Published
2023

3. 251-LB: Tracking Beta-Cell Regeneration in Human Pancreatic Slices using Adenovirus Transduction

Author

SILVIA ALVAREZ-CUBELA, DAGMAR KLEIN, CHARLES G. ALVER, MIRZA MUHAMMAD FAHD QADIR, LUCIANA MATEUS GONCALVES, FARHAN QURESHI, JOANA ALMACA, CHRISTOPHER FRAKER, ALEJANDRO CAICEDO, ALBERTO PUGLIESE, CAMILLO RICORDI, ELISA OLTRA, ASHUTOSH G. AGARWAL, RICARDO PASTORI, and JUAN DOMÍNGUEZ-BENDALA

Subjects
Endocrinology, Diabetes and Metabolism, Internal Medicine
Abstract

Introduction: The study of pancreatic regeneration would benefit greatly from the design and validation of robust human-based models. Human Pancreatic Slices (HPSs) are thin organotypic sections of live pancreatic tissue. The sectioning method preserves the overall histological structure of the organ, maintaining the integrity of the extracellular matrix and the natural interaction between the endocrine and exocrine compartments, as well as the local neural, vascular and immune milieu. Conditions for the long-term culture of HPSs, recently reported by our team, have enabled the real-time analysis of beta-cell neogenesis using adenoviral (AV) co-transduction of a red-green reporter and an insulin tracer in human pancreatic slices. However, the ability of these cells to respond to glucose was not established at that time. Methods: To determine whether new INS+ cells respond to glucose, we have designed an AV in which INS-dependent recombination leads to the expression of a blue marker (moxBFP) and a Calcium Imaging Reporter (gcAMP6s, green) whose intensity is proportional to glucose-dependent INS secretion. This allowed us to monitor glucose-stimulated calcium influx in the newly created cells. To track the generation of new beta cells with an even higher degree of resolution we subsequently placed the transduced slices in a custom-made microfluid chip. Conclusions: This setting further allows for longitudinal functional analyses in precise and highly controlled experimental conditions. Our ability to study regeneration in a clinically meaningful model represents a groundbreaking advance that may fast-track the screening and preclinical development of therapeutic agents. Disclosure S. Alvarez-cubela: n/a. A. Pugliese: Advisory Panel; Provention Bio, Inc., Consultant; Quell Tx. C. Ricordi: Advisory Panel; Vertex Pharmaceuticals Incorporated. E. Oltra: None. A. Pugliese: Advisory Panel; Provention Bio, Inc., Consultant; Quell Tx. A. G. Agarwal: None. R. Pastori: None. J. Domínguez-bendala: None. D. Klein: None. C. G. Alver: None. M. Qadir: None. L. Mateus goncalves: None. F. Qureshi: None. J. Almaca: None. C. Fraker: None. A. Caicedo: None. Funding National Institutes of Health (RO1DK130846UO1DK12393)

Published
2022

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