Search

Over the past years, several methods have been developed for gene cloning. Choosing a cloning strategy depends on various factors, among which simplicity and affordability have always been considered. The aim of this study, on the one hand, is to simplify gene cloning by skipping in vitro assembly reactions and, on the other hand, to reduce costs by eliminating relatively expensive materials. We investigated a cloning system using Escherichia coli harboring two plasmids, pLP-AmpR and pScissors-CmR. The pLP-AmpR contains a landing pad (LP) consisting of two genes (λ int and λ gam) that allow the replacement of the transformed linear DNA using site-specific recombination. After the replacement process, the inducible expressing SpCas9 and specific sgRNA from the pScissors-CmR (CRISPR/Cas9) vector leads to the removal of non-recombinant pLP-AmpR plasmids. The function of LP was explored by directly transforming PCR products. The pScissors-CmR plasmid was evaluated for curing three vectors, including the origins of pBR322, p15A, and pSC101. Replacing LP with a PCR product and fast-eradicating pSC101 origin-containing vectors was successful. Recombinant colonies were confirmed following gene replacement and plasmid curing processes. The results made us optimistic that this strategy may potentially be a simple and inexpensive cloning method. Key points: •The in vivo cloning was performed by replacing the target gene with the landing pad. •Fast eradication of non-recombinant plasmids was possible by adapting key vectors. •This strategy is not dependent on in vitro assembly reactions and expensive materials. [ABSTRACT FROM AUTHOR]

The accumulation of phase-transformation-induced strain during the direct thermoelastic martensitic transformation and structural strain in the course of loading under martensitic inelasticity conditions in three directions with respect to the rolling direction (0°, 45°, 90°) has been experimentally studied for a thin titanium nickelide plate. For the case of uniaxial tension, approximation functions are proposed to accurately describe direct transformation and martensitic inelasticity diagrams with stable values of material parameters. For all directions in the plate plane, the direct transformation diagram is located higher than the martensitic inelasticity diagram along the strain axis. [ABSTRACT FROM AUTHOR]

ABSTRACTSesame (Sesamum indicum L.) is an important oil crop and one of the oldest-known oil crops to humankind. Sesame has excellent nutritional and therapeutic properties; it is rich in important fatty acids, protein, fiber, and vital minerals. Oil percentage varies among different genotypes but generally accounts for more than 50% of the seed’s dry weight. To meet the increasing demand for vegetable oil production worldwide, expanding the cultivation of oil crops in newly reclaimed areas worldwide is essential. Molecular breeding is an expeditious approach for varietal improvement but requires efficient transgenesis. Published sesame transformation methods are highly genus-specific, tedious, and involve preparing and testing different media and explants. We produced transgenic sesame plants using a stable, noninvasive, and robust Agrobacterium tumefaciens transformation method. Leaves and flowers excised from the T0 plants at different developmental stages were PCR screened, and 61/93 seedlings were found to be PCR positive. T1 seeds resulting from two lines were germinated in a biocontainment greenhouse facility and screened using PCR, basta leaf painting, and spraying fully matured plants with basta herbicide (0.02 mg/l); non-transgenic segregants and control non-transgenic plants were severely damaged, and eventually died, while transgenic plants were not affected by the Basta spraying. RT-PCR on T1 plants indicated the presence of Bar transcripts in T1 progeny. Furthermore, RT-PCR using NPTII primers did not result in any amplification in transgenic sesame plants (NPTII is present in the vector but not in the T-DNA region) indicating that the transgenic sesame plants were not an Agrobacterium-contaminant.

A one-dimensional structural-simulation model for the deformation of shape memory alloys during thermoelastic phase transformations is used to describe the deformation change during the direct transformation under reversible stresses and the reversible shape memory effect. [ABSTRACT FROM AUTHOR]

A one-dimensional structural-simulation model is proposed to describe the deformation of shape memory alloys during thermoelastic phase transformations; it takes into account the nucleation and development of martensitic mesoelements during the direct transformation and their degradation and disappearance during the reverse transformation. An adequate description of the orientational transformation effects in terms of the model is demonstrated. [ABSTRACT FROM AUTHOR]

Based on the iron-based catalysts for direct synthesis of aromatics from syngas, the research progress of modified iron-based catalysts and iron-based zeolites composite catalysts was summarized, and the reaction mechanism was discussed; the coupling mode of the active components of the iron-based and zeolites composite catalyst, the modification of the iron-based catalyst, and the influence of the acidity and structural modification of the zeolites on the yield of aromatics were emphatically described; finally, the future research directions of iron-based catalysts were prospected. [ABSTRACT FROM AUTHOR]

Intertextuality, a term coined by Julia Kristeva, is a crucial aspect of all forms of creative expression, including literature, music, and art. The idea of intertextuality has been further expanded by Gérard Genette, who included intertextuality as one of the five aspects of the broader phenomenon of transtextuality. Among these several types is hypertextuality, which refers to the relationship between a text and its hypertextual extensions or variations. Within the scope of hypertextuality, Genette identified two methods of transforming a text A into a text B: direct and indirect transformation. This paper considers direct transformation. Although its primary association is with prose and poetry, it can also be extended to encompass song lyrics. In this article, several rock covers serve as examples of text B, and consideration is given to how they differ from their original counterparts (text A), albeit not in a musical form, but rather in terms of their lyrics. In the process, four different approaches were found for delivering a transformation of the original lyrics: fixed transformation, slight transformation, conversion of both the original meaning and lyrics, and considerable transformation. [ABSTRACT FROM AUTHOR]

Even though trait introgression for maize is relatively straightforward, conversion from a single germplasm source, native or gene edit, can greatly slow the development of commercial lines because the allele donor will also contribute undesirable parts of its genome (genetic drag) to the destination genome. Should evaluations of genome edits follow the GM assessment framework, which is event-based and can include comprehensive molecular, protein safety, environmental safety, and agronomic assessments, this will severely limit accessibility of important trait improvements and innovations to farmers and consumers even in a trait-based regulation environment due to the costly and heavy regulatory burden associated with GM approvals ([2]). How genome editing can fundamentally change the path for crop development Potential considerations for direct germplasm editing and trait-based regulation of genome edits One key consideration for trait-based regulation of genome edits is a rigorous product development pipeline. [Extracted from the article]

Copyright of Journal of Central South University is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Sesame (Sesamum indicum L.) is an important oil crop and one of the oldest-known oil crops to humankind. Sesame has excellent nutritional and therapeutic properties; it is rich in important fatty acids, protein, fiber, and vital minerals. Oil percentage varies among different genotypes but generally accounts for more than 50% of the seed's dry weight. To meet the increasing demand for vegetable oil production worldwide, expanding the cultivation of oil crops in newly reclaimed areas worldwide is essential. Molecular breeding is an expeditious approach for varietal improvement but requires efficient transgenesis. Published sesame transformation methods are highly genus-specific, tedious, and involve preparing and testing different media and explants. We produced transgenic sesame plants using a stable, noninvasive, and robust Agrobacterium tumefaciens transformation method. Leaves and flowers excised from the T0 plants at different developmental stages were PCR screened, and 61/93 seedlings were found to be PCR positive. T1 seeds resulting from two lines were germinated in a biocontainment greenhouse facility and screened using PCR, basta leaf painting, and spraying fully matured plants with basta herbicide (0.02 mg/l); non-transgenic segregants and control non-transgenic plants were severely damaged, and eventually died, while transgenic plants were not affected by the Basta spraying. RT-PCR on T1 plants indicated the presence of Bar transcripts in T1 progeny. Furthermore, RT-PCR using NPTII primers did not result in any amplification in transgenic sesame plants (NPTII is present in the vector but not in the T-DNA region) indicating that the transgenic sesame plants were not an Agrobacterium-contaminant. [ABSTRACT FROM AUTHOR]

The dependence of the stress of the onset of structural deformation of titanium nickelide on the degree of reverse phase transformation occurring after complete direct martensitic transformation is experimentally investigated. The experimental results are described qualitatively and quantitatively correctly in terms of a model, in which the maximum intrinsic inelastic strain intensity of the martensitic part of the representative volume of a shape memory alloy is used as an isotropic hardening parameter. [ABSTRACT FROM AUTHOR]

Copyright of Hydrographic Surveying & Charting / Haiyang Cehui is the property of Hydrographic Surveying & Charting Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)