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Colorectal cancer is the third most frequently diagnosed cancer in the world. Despite extensive studies and apparent progress in modern strategies for disease control, the treatment options are still not sufficient and effective, mostly due to frequently encountered resistance to immunotherapy of colon cancer patients in common clinical practice. In our study, we aimed to uncover the CCL9 chemokine action employing the murine model of colon cancer to seek new, potential molecular targets that could be promising in the development of colon cancer therapy. Mouse CT26.CL25 colon cancer cell line was used for introducing lentivirus-mediated CCL9 overexpression. The blank control cell line contained an empty vector, while the cell line marked as CCL9+ carried the CCL9-overexpressing vector. Next, cancer cells with empty vector (control) or CCL9-overexpressing cells were injected subcutaneously, and the growing tumors were measured within 2 weeks. Surprisingly, CCL9 contributed to a decline in tumor growth in vivo but had no effect on CT26.CL25 cell proliferation or migration in vitro. Microarray analysis of the collected tumor tissues revealed upregulation of the immune system-related genes in the CCL9 group. Obtained results suggest that CCL9 reveals its anti-proliferative functions by interplay with host immune cells and mediators that were absent in the isolated, in vitro system. Under specific study conditions, we determined unknown features of the murine CCL9 that have so far bee reported to be predominantly pro-oncogenic.

Introduction The goal of the study was to analyse the attitudes towards moral values adopted by Polish physiotherapists. Eight basic moral values were enumerated, namely: professionalism, care, fairness, dignity, autonomy, responsibility, trust, and profes�sional integrity. The chosen values were analysed taking into account the sex, age, education, work experience, workplace and the knowledge of moral rules included in the Code of Professional Ethics of the Physiotherapist of the Republic of Poland and/or in the Rules of Professional Ethics of Physiotherapists (the Polish Chamber of Physiotherapists). Methods The study embraced 199 professionally active physiotherapists, with 5 surveys not filled in correctly, which left 194. Results Variables such as sex ( p < 0.001) and workplace ( p < 0.016) differentiate the respondents significantly in the context of the studied values. Physiotherapists with longer working experience also declare attitudes more closely based on moral values than physiotherapists with shorter working experience ( p = 0.021). With regard to the adopted attitudes towards values, vital differences between men and women can be observed. Women choose fairness ( p < 0.001) whereas men choose the ethics of care ( p = 0.619). Physiotherapists employed by non-public centres are more oriented towards autonomous decisions than physiotherapists employed by publicly financed centres ( p = 0.048). Conclusions Numerous factors have been observed differentiating the attitudes of the respondents towards moral values. The differences may evidence a lack of uniform professional culture which might in turn translate into limited respect on the part of society. The variables differentiating the group are modal, thanks to which a change in the moral attitudes of Polish physiothera�pists is possible.