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AbstractThis study explores cognitive presence (CP) during remote learning by applying content analysis to English language learners’ (ELLs) asynchronous video discussions. The study draws on the framework of Community of Inquiry (CoI) and a four-phase inquiry called the Practical Inquiry Model (PIM). ELLs participated in weekly video discussions over the course of a semester using Flip, a video-based asynchronous computer-mediated communication (ACMC) tool. The data for this study include main reflection videos (N = 137) and peer-response videos (N = 153), and the content analysis provided an understanding to develop deeper learning and co-constructing meaning and knowledge. The findings offer a critical reflection on using asynchronous video discussion for co-constructing knowledge and enhancing social interaction. [ABSTRACT FROM AUTHOR]

The use of fluorescence in imaging has been a pivotal factor in advancing our scientific understanding. As microscopy continues to evolve, the terminology used to describe these techniques becomes increasingly complex, often resulting in a bewildering array of acronyms that resemble alphabet soup. Among the most prominent acronyms are those associated with advanced fluorescence microscopy techniques: Fluorescence Loss in Photobleaching (FLIP), Fluorescence Localization after Photobleaching (FLAP), Fluorescence Recovery after Photobleaching (FRAP), Förster Resonance Energy Transfer (FRET), and Fluorescence Lifetime Imaging Microscopy (FLIM). Each of these methods provides invaluable insights into molecular dynamics and interactions within living cells and tissues. This article, Part 2 of the Alphabet Soup of Microscopy series, aims to clarify these widely used fluorescence microscopy techniques and to illuminate their contributions to our understanding of cellular processes. [ABSTRACT FROM AUTHOR]

This article reports the results of an explorative, quantitative study designed to investigate the pedagogical potential of Flip (formerly Flipgrid) as a connectivist and transmedia tool for developing English language learners’ productive skills to provide a basis for their continued improvement. The study suggests that the connectivist practice via Flip provides significant effect on improving secondary school students’ productive skills. A total of 39 students, divided randomly into one experimental group and one control group, participated in the study during the eight-week curriculum involving an English course. Participants in the experimental group (n = 19) used Flip as a learning tool, whereas the control group participants (n = 20) followed the same curriculum without Flip. Pre- and posttests were used to compare the participants’ speaking and writing productions before and after the project. These tests were evaluated based on a comparison of their mean scores by SPSS (Statistical Package for the Social Sciences) software. We used Questionnaires to analyze the positive and negative aspects of Flip. An independent samples t-test revealed that the experimental group outperformed the control group and showed the potential of regular Flip narrative-based tasks for developing English language learners’ productive skills (p ≤ 0.05 = 0.039). Thus, Flip can be used by teachers and learners to improve speaking and writing, and thus overcome the limitations on time, practice, and feedback inherent in classroom-based courses. [ABSTRACT FROM AUTHOR]

Purpose of Review: Esophagogastric junction outflow obstruction (EGJOO), defined manometrically by impaired esophagogastric junction relaxation (EGJ) with preserved peristalsis, can be artifactual, due to secondary etiologies (mechanical, medication-induced), or a true motility disorder. The purpose of this review is to go over the evolving approach to diagnosing and treating clinically relevant EGJOO. Recent Findings: Timed barium esophagram (TBE) and the functional lumen imaging probe (FLIP) are useful to identify clinically relevant EGJOO that merits lower esophageal sphincter (LES) directed therapies. There are no randomized controlled trials evaluating EJGOO treatment. Uncontrolled trials show effectiveness for pneumatic dilation and peroral endoscopic myotomy to treat confirmed EGJOO; Botox and Heller myotomy may also be considered but data for confirmed EGJOO is more limited. Summary: Diagnosis of clinically relevant idiopathic EGJOO requires symptoms, exclusion of mechanical and medication-related etiologies, and confirmation of EGJ obstruction by TBE or FLIP. Botox LES injection has limited durability, it can be used in patients who are not candidates for other treatments. PD and POEM are effective in confirmed EGJOO, Heller myotomy may also be considered but data for confirmed EGJOO is limited. Randomized controlled trials are needed to clarify optimal management of EGJOO. [ABSTRACT FROM AUTHOR]

In this article, we examine Differential Fault Attacks (DFA) targeting two stream ciphers, FLIP and FiLIP. We explore the fault model where an adversary flips a single bit of the key at an unknown position. Our analysis involves establishing complexity bounds for these attacks, contingent upon the cryptographic parameters of the Boolean functions employed as filters and the key size. Initially, we demonstrate how the concept of sensitivity enables the detection of the fault position using only a few keystream bits. This represents an enhancement over previous DFA methodologies applied to these ciphers. Subsequently, we leverage the properties of the filter's derivatives to execute attacks. This approach is universally applicable to any filter, and we delineate specific attack strategies for the two function families previously implemented in these ciphers. [ABSTRACT FROM AUTHOR]

INTRODUCTION: Guidelines advise esophageal motility testing for dysphagia when structural disorders are ruled out, but cost concerns impede adoption. We evaluated cost-effective positioning of esophageal motility testing in the algorithm to evaluate esophageal dysphagia. METHODS: We developed a decision analytic model comparing 3 strategies: (i) esophageal manometry, (ii) screening impedance planimetry followed by esophageal manometry if needed, or (iii) nonalgorithmic usual care. Diagnostic test accuracy was adapted to expected rates of esophageal motility disorders in general gastroenterology populations. We modeled routine testing for all patients with nonstructural/ mechanical dysphagia compared with selective testing with strong suspicion for achalasia. Cost outcomes were defined on national commercial and Medicare datasets stratified on age and sex. Health outcomes were modeled on populations with achalasia. The time horizon was 1 year. RESULTS: Motility testing was preferred over nonalgorithmic usual care due to cost savings rather than health gains. To commercial insurers, routine esophageal manometry for nonstructural/mechanical dysphagia would be cost-saving below a reimbursed cost of $2,415. Screening impedance planimetry would be cost saving below a reimbursed cost of $1,130. The limit for reimbursed costs would be lower for patients older than 65 years to achieve cost savings mainly due to insurance. Sex did not significantly influence cost-effectiveness. Patients and insurers preferred routine screening impedance planimetry before manometry when the index of suspicion for achalasia was below 6%. DISCUSSION: Aligningwith practice guidelines, routine esophageal motility testing seems cost saving to patients and insurers compared with nonalgorithmic usual care to evaluate nonstructural/mechanical dysphagia. Choice of testing should be guided by index of suspicion. [ABSTRACT FROM AUTHOR]

Dysphagia is a common symptom with significant impact on quality of life. Our diagnostic armamentarium was primarily limited to endoscopy and barium esophagram until the advent of manometric techniques in the 1970s, which provided the first reliable tool for assessment of esophageal motor function. Since that time, significant advances have been made over the last 3 decades in our understanding of various esophageal motility disorders due to improvement in diagnostics with high-resolution esophageal manometry. High-resolution esophageal manometry has improved the sensitivity for detecting achalasia and has also enhanced our understanding of spastic and hypomotility disorders of the esophageal body. In this review, we discuss the current approach to diagnosis and therapeutics of various esophageal motility disorders.

Abstract After the occurrence of a large earthquake, engineering seismologists are often requested to estimate strong ground motions at a site where strong motion data were not obtained. The goal of this study was to test the ability of a class of methods that uses Fourier phase characteristics for the post-earthquake ground motion estimation, making use of the precious opportunity provide by the ESG6 Blind Prediction Steps 2&3. It was also part of the goal of this study to test the performance of the effective stress analyses to account for soil nonlinearity. In addition to the dataset provided by the organizer of the blind prediction, the author used additional accelerometric data from a nearby JMA site. To simulate ground motions for an M5.9 earthquake at the target site “KUMA”, the Fourier amplitude spectrum was estimated from the spectral ratio between KUMA and the nearby JMA site. The Fourier phase spectrum was approximated by the spectrum of another event at KUMA. Comparison between the estimated and recorded ground motions after the blind prediction revealed that the estimated ground motions were fairly consistent with the observed ground motions, indicating the effectiveness of the method when the rupture process of the target event is simple and the soil nonlinearity at the target site is not significant. To simulate ground motions at KUMA for the M6.5 foreshock and the M7.3 mainshock of the 2016 Kumamoto earthquake sequence, the author conducted effective stress analyses using a program called “FLIP” to account for soil nonlinearity. Comparison between the estimated and recorded ground motions after the blind prediction indicated that the low-frequency components were overestimated and the high-frequency components were underestimated. The strong soil nonlinearity considered in the effective stress analyses was the main cause of the discrepancy. One explanation for this result could be that the nonlinear soil behavior at KUMA during the foreshock and the mainshock was not a strong one. Another explanation could be that the effect of soil nonlinearity was already included in the records at JMA and the effect of soil nonlinearity was double counted in the results submitted by the author. Graphical Abstract

This study is set in EFL teacher training during the COVID -19 pandemic. The goal of this research study was to examine students' perceptions of the development of their oral abilities by means of weekly Flip video recordings. An action research design was carried out for EFL teacher-training students from a Chilean university. Synchronous English language sessions focusing on oral production were aided by asynchronous practice on the Flip platform. Content analysis was conducted to examine the students' perceptions of the use of Flip to facilitate the development of speaking skills. Cooccurring categories were identified, and related networks were produced to identify relevant nodes. Findings revealed favorable perceptions in both the linguistic and socioaffective aspects of learners' oral performance resulting from the weekly recordings. It was concluded that the use of Flip promoted the students' oral production by heightened positive socioaffective perceptions over the course of one academic semester during the time of COVID-19. [ABSTRACT FROM AUTHOR]

International students, particularly international English as a Second Language (ESL) students who come to the United States with relatively lower levels of English oral language proficiency, are often reluctant to participate in academic discussions. In a graduate-level TESOL program with a majority of international ESL students, a video recording, sharing, and discussion platform, Flip, was utilized in the program's teaching innovation. Flip is widely adopted by educators of all grade levels worldwide, as it enables educators to create a less formal online space to meet the holistic needs of international ESL students, including both academic and social needs. The article explains the teaching context of the practice, four stages of course integration with Flip, reasons for the integration, reflections, and future pedagogical directions. Incorporating Flip in this program promotes linguistic benefits, teacher identity and skill development, a community of belonging, and increased equity in assessment. Practical pedagogical considerations are also provided at the end of the article. [ABSTRACT FROM AUTHOR]

After the occurrence of a large earthquake, engineering seismologists are often requested to estimate strong ground motions at a site where strong motion data were not obtained. The goal of this study was to test the ability of a class of methods that uses Fourier phase characteristics for the post-earthquake ground motion estimation, making use of the precious opportunity provide by the ESG6 Blind Prediction Steps 2&3. It was also part of the goal of this study to test the performance of the effective stress analyses to account for soil nonlinearity. In addition to the dataset provided by the organizer of the blind prediction, the author used additional accelerometric data from a nearby JMA site. To simulate ground motions for an M5.9 earthquake at the target site "KUMA", the Fourier amplitude spectrum was estimated from the spectral ratio between KUMA and the nearby JMA site. The Fourier phase spectrum was approximated by the spectrum of another event at KUMA. Comparison between the estimated and recorded ground motions after the blind prediction revealed that the estimated ground motions were fairly consistent with the observed ground motions, indicating the effectiveness of the method when the rupture process of the target event is simple and the soil nonlinearity at the target site is not significant. To simulate ground motions at KUMA for the M6.5 foreshock and the M7.3 mainshock of the 2016 Kumamoto earthquake sequence, the author conducted effective stress analyses using a program called "FLIP" to account for soil nonlinearity. Comparison between the estimated and recorded ground motions after the blind prediction indicated that the low-frequency components were overestimated and the high-frequency components were underestimated. The strong soil nonlinearity considered in the effective stress analyses was the main cause of the discrepancy. One explanation for this result could be that the nonlinear soil behavior at KUMA during the foreshock and the mainshock was not a strong one. Another explanation could be that the effect of soil nonlinearity was already included in the records at JMA and the effect of soil nonlinearity was double counted in the results submitted by the author. [ABSTRACT FROM AUTHOR]

This study evaluated the perceived impact of using Flip (Microsoft Corp.) to facilitate a group project in a socially distanced environment. Student groups were assigned a self-care scenario, and students independently traveled to local pharmacies to identify a pertinent nonprescription product. Students individually recorded a review of the product using Flip. Subsequently, students reviewed and replied to group member videos to compare products. Students worked as a group to develop consensus regarding the most appropriate product recommendation for their scenario. At semester end, students completed a questionnaire about their experiences using Flip for the project. Sixty-eight students (91%) completed the perceptions questionnaire at course conclusion. Students found Flip enjoyable (68%) and easy to use (74%). The majority of students (82%) viewed all of their group members' Flip videos, and most (88%) agreed or strongly agreed Flip facilitated group work in a socially distanced environment. Students overall perceived Flip as enjoyable and easy to use. Though utilization of Flip was initially due social distancing requirements, the positive student feedback encouraged continued use of this platform asynchronously. Flip provides a unique mechanism for facilitating group projects in multiple different environments. [ABSTRACT FROM AUTHOR]

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Finding creative and effective ways to support student experiences and student learning in the classroom is an important goal for instructors, especially as remote and hybrid formats become more common and new, educational technologies are recommended. The current work involved the creation and implementation of a curricular innovation using Flip to encourage the use of evidence-based learning strategies and promote a sense of community in a college classroom. A survey was developed and administered as extra credit in a large Introduction to Psychology class in Spring 2021, with questions focused on examining student perceptions of using Flip in the classroom. Results revealed that students enjoyed using Flip for assignments, believed that Flip assignments helped their learning of class content, and recommended the instructor keeping using Flip in future semesters. The current work highlights the versatility and benefit of using Flip as an instructional tool in the classroom. [ABSTRACT FROM AUTHOR]

With the advantages of high flexibility, strong adaptability, etc., the legged robot can help humans to complete numerous complicated tasks. In this paper, a kind of reconfigurable legged robot with a flexible waist was proposed. Compared with the common robots with a rigid trunk, the proposed robot can twist its waist flexibly. Through analysis, it is found that the flexible waist can improve the trunk workspace, foot endpoints' workspace and static stability margin of the robot, and further enhance the motion performance of the robot. Meanwhile, by imitating the creatures in nature, the motion gait planning of the robot was provided. Additionally, the proposed robot has excellent reconfigurable characteristics, and can flexibly transform among three forms to adapt to different working environments and accomplish different tasks. Further, after capsizing, the robot can complete the motion of turning over more easily. In this paper, the reconfiguration posture and the motion of turning over of the robot were planned in detail, and finally verified by ADAMS simulation. [ABSTRACT FROM AUTHOR]

Several combinatorial actions of the affine Weyl group of type C ˜ n on triangulations, trees, words and permutations are compared. Addressing a question of David Vogan, we show that, modulo a natural involution, these permutation representations are multiplicity-free. The proof uses a general construction of Gelfand subgroups in the affine Weyl groups of types C ˜ n and B ˜ n. [ABSTRACT FROM AUTHOR]

The ongoing co-circulation of multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains necessitates advanced methods such as high-throughput multiplex pseudovirus systems for evaluating immune responses to different variants, crucial for developing updated vaccines and neutralizing antibodies (nAbs). We have developed a quadri-fluorescence (qFluo) pseudovirus platform by four fluorescent reporters with different spectra, allowing simultaneous measurement of the nAbs against four variants in a single test. qFluo shows high concordance with the classical single-reporter assay when testing monoclonal antibodies and human plasma. Utilizing qFluo, we assessed the immunogenicities of the spike of BA.5, BQ.1.1, XBB.1.5, and CH.1.1 in hamsters. An analysis of cross-neutralization against 51 variants demonstrated superior protective immunity from XBB.1.5, especially against prevalent strains such as "FLip" and JN.1, compared to BA.5. Our finding partially fills the knowledge gap concerning the immunogenic efficacy of the XBB.1.5 vaccine against current dominant variants, being instrumental in vaccine-strain decisions and insight into the evolutionary path of SARS-CoV-2., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

The uncontrolled proliferation of malignant cells in growing tumors results in the generation of different stressors in the tumor microenvironment, such as nutrient shortage, hypoxia and acidosis, among others, that disrupt endoplasmic reticulum (ER) homeostasis and may lead to ER stress. As a response to ER stress, both normal and tumor cells launch a set of signaling pathways known as the unfolded protein response (UPR) to restore ER proteostasis and maintain cell viability and function. However, under sustained ER stress, an apoptotic cell death process can be induced and this has been the subject of different review articles, although the role of the TRAIL-R2/DR5-activated extrinsic pathway of apoptosis has not yet been thoroughly summarized. In this Review, we provide an updated overview of the molecular mechanisms regulating cell fate decisions in tumor cells undergoing ER stress and discuss the role of the tumor necrosis factor (TNF)-related apoptosis-inducing ligand receptor 2 (TRAIL-R2/DR5) in the final outcome of UPR signaling. Particularly, we focus on the mechanisms controlling cellular FLICE-like inhibitory protein (FLIP) levels in tumor cells undergoing ER stress, which may represent a potential target for therapeutic intervention in cancer. [ABSTRACT FROM AUTHOR]

Phytoplankton patterns have been observed widely in aquatic systems. Although pattern formation has been investigated based on many PDEs, discrete models on aquatic systems can provide more complex dynamics. A discrete toxic-phytoplankton–zooplankton model is studied in this paper, with the consideration of Allee effect and cross-diffusion. Focusing on Allee effect coefficient, flip and Neimark–Sacker bifurcation analyses are carried out. And focusing on cross-diffusion coefficient, Turing bifurcation analyses are carried out. Parameter conditions and bifurcation diagram of these bifurcations are obtained correspondingly. Numerical simulations are then performed which are consistent with results of theoretical analysis. Irregular patterns can be formed by flip bifurcation. Spirals can be formed by Neimark–Sacker bifurcation. Spots and stripes can be formed by Turing bifurcation. When Turing and flip, or Turing and Neimark–Sacker bifurcations both occur, special patterns can be obtained. [ABSTRACT FROM AUTHOR]

Introduction: The esophagogastric junction (EGJ) is a complex anti-reflux barrier whose integrity relies on both the intrinsic lower esophageal sphincter (LES) and extrinsic crural diaphragm. During hiatal hernia repair, it is unclear whether the crural closure or the fundoplication is more important to restore the anti-reflux barrier. The objective of this study is to analyze changes in LES minimum diameter (Dmin) and distensibility index (DI) using the endoluminal functional lumen imaging probe (FLIP) during hiatal hernia repair. Methods: Following implementation of a standardized operative FLIP protocol, all data were collected prospectively and entered into a quality database. This data were reviewed retrospectively for all patients undergoing hiatal hernia repair. FLIP measurements were collected prior to hernia dissection, after hernia reduction, after cruroplasty, and after fundoplication. Additionally, subjective assessment of the tightness of crural closure was rated by the primary surgeon on a scale of 1 to 5, 1 being the loosest and 5 being the tightest. Results: Between August 2018 and February 2020, 97 hiatal hernia repairs were performed by a single surgeon. FLIP measurements collected using a 40-mL volume fill without pneumoperitoneum demonstrated a significant decrease in LES Dmin (13.84 ± 2.59 to 10.27 ± 2.09) and DI (6.81 ± 3.03 to 2.85 ± 1.23 mm2/mmHg) after crural closure (both p < 0.0001). Following fundoplication, there was a small, but also statistically significant, increase in both Dmin and DI (both p < 0.0001). Additionally, subjective assessment of crural tightness after cruroplasty correlated well with DI (r = − 0.466, p < 0.001) and all patients with a crural tightness rating ≥ 4.5 (N = 13) had a DI < 2.0 mm2/mmHg. Conclusion: Cruroplasty results in a significant decrease in LES distensibility and may be more important than fundoplication in restoring EGJ competency. Additionally, subjective estimation of crural tightness correlates well with objective FLIP evaluation, suggesting surgeon assessment of cruroplasty is reliable. [ABSTRACT FROM AUTHOR]

Let n , d ∈ N and n > d. An (n − d) -domino is a box I 1 × ⋯ × I n such that I j ∈ { [ 0 , 1 ] , [ 1 , 2 ] } for all j ∈ N ⊂ [ n ] with | N | = d and I i = [ 0 , 2 ] for every i ∈ [ n ] ∖ N. If A and B are two (n − d) -dominoes such that A ∪ B is an (n − (d − 1)) -domino, then A , B is called a twin pair. If C , D are two (n − d) -dominoes which form a twin pair such that A ∪ B = C ∪ D and { C , D } ≠ { A , B } , then the pair C , D is called a flip of A , B. A family D of (n − d) -dominoes is a tiling of the box [ 0 , 2 ] n if interiors of every two members of D are disjoint and ⋃ B ∈ D B = [ 0 , 2 ] n. An (n − d) -domino tiling D ′ is obtained from an (n − d) -domino tiling D by a flip, if there is a twin pair A , B ∈ D such that D ′ = (D ∖ { A , B }) ∪ { C , D } , where C , D is a flip of A , B. A family of (n − d) -domino tilings of the box [ 0 , 2 ] n is flip-connected, if for every two members D , E of this family the tiling E can be obtained from D by a sequence of flips. In the paper a flip-connected class of (n − d) -domino tilings of the box [ 0 , 2 ] n is described. [ABSTRACT FROM AUTHOR]

Dysphagia is a common symptom with significant impact on quality of life. Our diagnostic armamentarium was primarily limited to endoscopy and barium esophagram until the advent of manometric techniques in the 1970s, which provided the first reliable tool for assessment of esophageal motor function. Since that time, significant advances have been made over the last 3 decades in our understanding of various esophageal motility disorders due to improvement in diagnostics with high-resolution esophageal manometry. High-resolution esophageal manometry has improved the sensitivity for detecting achalasia and has also enhanced our understanding of spastic and hypomotility disorders of the esophageal body. In this review, we discuss the current approach to diagnosis and therapeutics of various esophageal motility disorders. [ABSTRACT FROM AUTHOR]

Eosinophilic esophagitis (EoE) is a leading cause of food bolus impaction in children and adults. The mechanism of dysphagia in EoE, particularly non-obstructive dysphagia, remains incompletely understood. While fibrostenotic processes appear to be critical in the development of dysphagia, somatosensory dysfunction and dysmotility also contribute. This review considers potential mechanisms of dysphagia and evaluates the utility of current and future treatment strategies in this context. [ABSTRACT FROM AUTHOR]

Background. There are growing interests from practicing endoscopists to implement the functional lumen imaging probe (FLIP) impedance planimetry system. We present a simulation-based curriculum using an "into the fire" approach with hands-on pre- and post-tests to teach the use of this technology. Methods. The curriculum consists of a series of pre-tests, didactic content, mentored hands-on instructions, and post-tests. Pre- and post-testing included a knowledge-based written test, a confidence survey, and an assessment form specific to the hands-on performance of FLIP. Result. Twenty-two practicing physicians completed the curriculum. After course completion, participants had improved knowledge-based written test scores from 6.8±1.7 to 8.9±0.9 (P <0.001), confidence scores from 10.0±5.9 to 22.1±2.6 (P <0.001), and hands-on performance score from 11.4±3.4 to 23.1±2.0 (P <0.001) with significant improvement in all components of the hands-on skills. Conclusion. Our simulation curriculum is effective in improving confidence, knowledge, and technical proficiency when teaching the use of FLIP to practicing physicians. [ABSTRACT FROM AUTHOR]

Single water swallow (SWS) high-resolution manometry (HRM) may miss relevant esophageal motility disorders. Solid test meal (STM) during HRM and lately the functional lumen imaging probe (FLIP) have been shown to be of diagnostic value in the assessment of motility disorders. We aimed to assess the diagnostic yield of STM and FLIP in non-obstructive dysphagia (NOD). Patients assessed for dysphagia with both HRM and FLIP between April 2016 and August 2019 were analyzed for signs of non-obstructive EGJ outflow obstruction (EGJOO) according to Chicago Classification 3.0 (CCv3) and CC adapted for the use with solid swallows (CC-S), followed by an individual group-specific analysis. Five subjects without dysphagia served as control group. Standard HRM- and FLIP-values as well as esophagograms and Eckardt Scores were analyzed. Forty-two patients were identified (male/female, 14/36, median age 62). Twenty-five (59.5%) were diagnosed with EGJOO during STM only (= SWS-negative patients; CC-S). The EGJ distensibility index (EGJ-DI) of symptomatic patients was significantly lower compared to the control group (p = 0.006). EGJ-DI was < 3mm2/mmHg in 67% and 88% of patients diagnosed according to CC-S and CCv3, respectively. The IRP during STM showed a significant association to the corresponding EGJ-DI values (p < 0.001). Seventy-six percent of patients received treatment because of additional STM evaluation with a favorable clinical response rate of 89%. STM and FLIP identify EGJOO in symptomatic patients with normal SWS during HRM. STM resembles an inexpensive and clinically meaningful option to diagnose motility disorders and helps to select patients for interventional treatment. [ABSTRACT FROM AUTHOR]

Let X be a normal Q-factorial projective variety with at most log canonical singularities. We shall give a sufficient condition for the existence of at most finitely many KX-negative extremal rays R(C NE(X) of divisorial type. As an application, we show that for a nonisomorphic surjective endomorphism f:X - X of a normal projective Q-factorial terminal 3-fold X with (X) > 0, a suitable power fk (k > 0) of f descends to a nonisomorphic surjective endomorphism g:Xmin - Xmin of a minimal model Xmin of X. [ABSTRACT FROM AUTHOR]

Background: Endoscopic per-oral pyloromyotomy (POP) has emerged as a safe and effective first line option in medically refractory gastroparesis. Determining the appropriate extent of the pyloromyotomy continues to present a challenge as there are no standardized tools for measuring changes in pyloric distensibility during the procedure. The objective of this study was to evaluate the utility of using impedance planimetry with endoscopic functional luminal imaging probe (FLIP) to measure changes in pyloric distensibility after POP, and to compare these changes with improvement in symptoms and objective gastric emptying. Methods: Patients with medically refractory gastroparesis underwent POP with FLIP measurements of the pylorus (EndoFLIP®, Medtronic, Fridley MN). FLIP measurements, as well as changes in symptoms measured by the validated gastroparesis cardinal symptom index (GCSI) and scintigraphic gastric emptying studies (GES), were evaluated before and after POP. Results: A total of 14 patients underwent measurement with FLIP during POP, 12 of whom had pre- and post-POP measurements. Mean pyloric diameter increased by 1.4 mm, from 13.9 mm to 15.3 mm (p = 0.0012). Mean distensibility index increased from 6.2 mm2/mmHg to 9.1 mm2/mmHg (p = 0.0074). Successful division of the pylorus was achieved in 100% of patients with a mean operative time of 36 min and no perioperative complications. The mean length of stay was 0.7 days (0–3 days). Post-POP mean GCSI score improved from 2.97 to 2.28 at a mean follow-up time of 27 days (p < 0.001). Objective improvement in gastric emptying was observed in 80% of patients with scintigraphic GES, with mean four-hour retention decreasing from 46.3% to 32.4% (p < 0.007). Conclusions: FLIP is a safe and feasible tool to provide objective measurements during POP. Larger cohorts with longer follow-up are required to determine if measured improvements in pyloric diameter and distensibility are predictive of sustained improvements in GCSI and GES. [ABSTRACT FROM AUTHOR]

In this article, we propose key recovery attack on two stream ciphers: Kreyvium and FLIP $_{530}(42,128,360)$ 530 (42 , 128 , 360) using Differential Fault Attack (DFA) technique. These two ciphers are being used in Fully Homomorphic Encryption (FHE) due to their low error growth during keystream generation. Kreyvium is an NFSR-based stream cipher and FLIP is a permutation-based stream cipher. We first show that the complete state of the Kreyvium can be recovered by injecting 3 faults and considering 450 many keystream bits. In case of FLIP, we show that if there is a 1-bit fault in the state of the cipher then from 9000 normal and faulty keystream bits the state (i.e., the secret key) of the cipher can be recovered. For single bit fault, one will require to solve a system of equations for each 530 possible fault locations to recover the correct key of FLIP. To the best of our knowledge, this is the first article which analyzes the security of these two FHE supported stream ciphers under DFA and it has been observed that DFA completely reveals the secret keys of these two ciphers with very minimal faults. [ABSTRACT FROM AUTHOR]

COVID-19 pandemic is casting a long shadow, and the appearance of the JN.1 variety calls attention to the necessity of maintaining heightened awareness. It considers the strength that has been developed via immunization programs and the necessity of global collaboration to find a solution in light of the emergence of new strains of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Phylogenetically, the SARS-CoV-2 Omicron XBB lineages, which include EG.5.1 and HK.3, are different from the SARS-CoV-2 BA.2.86 lineage, which was initially discovered in August 2023. More than 30 mutations in the spike (S) protein are carried by BA.2.86 compared to XBB and BA.2, suggesting a high potential for immune evasion. JN.1 (BA.2.86.1.1), a descendant of BA.2.86, appeared in late 2023 after the format had undergone evolution. JN.1 carries three mutations in proteins that do not include S, as well as S:L455S. As previously demonstrated, the HK.3 and other "FLip" variations possess the S:L455F mutation, which enhances transmissibility and immune escape capacity in comparison to the parental EG.5.1 variety. This mutation is a characteristic of JN.1. The COVID-19 virus is dynamic and evolves over time. New varieties can sometimes spread more quickly or effectively after these alterations. If that happens, the new variant has a chance to outpace the current varieties in terms of frequency., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

With the advantages of high flexibility, strong adaptability, etc., the legged robot can help humans to complete numerous complicated tasks. In this paper, a kind of reconfigurable legged robot with a flexible waist was proposed. Compared with the common robots with a rigid trunk, the proposed robot can twist its waist flexibly. Through analysis, it is found that the flexible waist can improve the trunk workspace, foot endpoints’ workspace and static stability margin of the robot, and further enhance the motion performance of the robot. Meanwhile, by imitating the creatures in nature, the motion gait planning of the robot was provided. Additionally, the proposed robot has excellent reconfigurable characteristics, and can flexibly transform among three forms to adapt to different working environments and accomplish different tasks. Further, after capsizing, the robot can complete the motion of turning over more easily. In this paper, the reconfiguration posture and the motion of turning over of the robot were planned in detail, and finally verified by ADAMS simulation.

The uncontrolled proliferation of malignant cells in growing tumors results in the generation of different stressors in the tumor microenvironment, such as nutrient shortage, hypoxia and acidosis, among others, that disrupt endoplasmic reticulum (ER) homeostasis and may lead to ER stress. As a response to ER stress, both normal and tumor cells launch a set of signaling pathways known as the unfolded protein response (UPR) to restore ER proteostasis and maintain cell viability and function. However, under sustained ER stress, an apoptotic cell death process can be induced and this has been the subject of different review articles, although the role of the TRAIL-R2/DR5-activated extrinsic pathway of apoptosis has not yet been thoroughly summarized. In this Review, we provide an updated overview of the molecular mechanisms regulating cell fate decisions in tumor cells undergoing ER stress and discuss the role of the tumor necrosis factor (TNF)-related apoptosis-inducing ligand receptor 2 (TRAIL-R2/DR5) in the final outcome of UPR signaling. Particularly, we focus on the mechanisms controlling cellular FLICE-like inhibitory protein (FLIP) levels in tumor cells undergoing ER stress, which may represent a potential target for therapeutic intervention in cancer.

Evolution of SARS-CoV-2 requires the reassessment of current vaccine measures. Here, we characterized BA.2.86 and XBB-derived variant FLip by investigating their neutralization alongside D614G, BA.1, BA.2, BA.4/5, XBB.1.5, and EG.5.1 by sera from 3-dose-vaccinated and bivalent-vaccinated healthcare workers, XBB.1.5-wave-infected first responders, and monoclonal antibody (mAb) S309. We assessed the biology of the variant spikes by measuring viral infectivity and membrane fusogenicity. BA.2.86 is less immune evasive compared to FLip and other XBB variants, consistent with antigenic distances. Importantly, distinct from XBB variants, mAb S309 was unable to neutralize BA.2.86, likely due to a D339H mutation based on modeling. BA.2.86 had relatively high fusogenicity and infectivity in CaLu-3 cells but low fusion and infectivity in 293T-ACE2 cells compared to some XBB variants, suggesting a potentially different conformational stability of BA.2.86 spike. Overall, our study underscores the importance of SARS-CoV-2 variant surveillance and the need for updated COVID-19 vaccines. [Display omitted] • BA.2.86 is less immune evasive compared to FLip and other XBB variants • BA.2.86 is antigenically more similar to BA.2 and BA.4/5 than XBB variants • MAb S309 is unable to neutralize BA.2.86 possibly contributed by a D339H mutation • The fusion and infectivity of BA.2.86 is higher than XBB variants in CaLu-3 cells The SARS-CoV-2 BA.2.86 variant is less resistant to neutralization by bivalent vaccine-induced antibodies compared to FLip and other XBB variants but more resistant to mAb S309. BA.2.86 shows higher fusogenicity and infectivity in CaLu-3 cells compared to that in 293T-ACE2 cells. [ABSTRACT FROM AUTHOR]

Oral demonstration of knowledge is an effective learning and assessment strategy. It has been shown that generating explanations to oneself, or self-explaining, can improve student understanding of information. This can be achieved via student-generated videos. The quantitative effects of student-generated videos on learning and assessment in postsecondary education are unknown. To our knowledge, this is the first study to analyze the effects asynchronous student-generated videos have on student learning and assessment in a large-enrollment (∼400 students), undergraduate physiology course. Students were charged with making self-generated videos discussing major physiological concepts and uploading these videos to Flip for assessment. Flip is an online, social education platform for asynchronous video-based discussion. In the present study, we combined four semesters ( n = 1,100 students) of Flip data and analyzed the effects it had on student examination performance. Specifically, we first analyzed how students performed on exam questions corresponding to their Flip prompts in comparison to students not assigned those prompts [25/44 (57%) were statistically significantly different]. Second, we analyzed the association between Flip prompt score and performance on corresponding exam questions [39/44 (89%) were statistically significantly different]. Third, we analyzed the association between cumulative Flip score and performance on all corresponding, and noncorresponding exam questions. Finally, we analyzed the association between cumulative Flip score and averaged exam performance. There was a positive association ( r = 0.54). Taken together, our data suggest that asynchronous student-generated Flip videos can facilitate student learning and assessment in a large-enrollment, undergraduate physiology course. NEW & NOTEWORTHY Oral demonstration of knowledge is an effective learning and assessment strategy. Student-generated videos have been shown to improve learning and assessment in secondary education. To our knowledge, this is the first study to analyze the effects asynchronous student-generated Flip videos have on student learning and assessment in postsecondary education. The results of the present study suggest that asynchronous student-generated Flip videos can facilitate student learning and assessment in a large-enrollment (∼400 students), undergraduate physiology course.

As an opportunistic causative pathogen of "Visceral White Spot Disease" in several kinds of teleost, Pseudomonas plecoglossicida has caused huge economic losses to aquaculture. Flagella is the motility organ of bacteria as well as a vital virulence factor. The protein encoded by the fliP gene is involved in the formation of the flagellar type III secretion export apparatus, which plays a crucial role in flagellar assembly. In this study, the Δ fliP and C-Δ fliP strains of P. plecoglossicida were constructed to explore the biological profiles mediated by the fliP gene and its effects on the virulence of this organism. Compared to the wild type strain (NZBD9) of P. plecoglossicida , the deletion strain Δ fliP exhibited a significant impairment in swimming motility, chemotaxis, adhesion, and biofilm formation abilities. The transcriptomic analysis of the NZBD9, Δ fliP and C-Δ fliP strains revealed that the fliP gene may affect the biological profiles of P. plecoglossicida by down-regulating the expression of flagellar assembly, type 6 secretion system and bacterial chemotaxis related genes. Furthermore, the results of artificial infection showed that the virulence of Δ fliP strain to hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) was attenuated, with a LD 50 value of 2.5 × 104 CFU/fish. These findings demonstrate that the fliP gene contributes to the virulence of P. plecoglossicida by regulating its motility. • Δ fliP strain was constructed by a two-step allelic exchange from P. plecogerossicida NZBD9 strain. • Deletion of fliP significantly reduced pathogenicity of P. plecoglossicida to hybrid grouper. • Δ fliP exhibited a significant impairment in swimming motility, chemotaxis, adhesion, and biofilm formation abilities. • Δ fliP down-regulated the expression of flagellar assembly, type 6 secretion system and bacterial chemotaxis related genes. [ABSTRACT FROM AUTHOR]

Pseudomonas plecoglossicida is a pathogen that causes visceral white spot disease in a variety of teleosts. The protein encoded by fliP gene is involved in the assembly of bacterial flagella, which plays a vital role in bacterial pathogenicity. However, the roles of the fliP gene on the host immune response remain unclear. Here, we compared the pathogenicity of fliP gene-deleted (Δ fliP) strain, fliP gene-complemented (C-Δ fliP) strain and wild-type (NZBD9) strain of P. plecoglossicida to hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂), and explored the impacts of fliP gene on the immune response of hybrid grouper to P. plecoglossicida infection by using RNA-seq. In this study, the grouper in the Δ fliP strain-infected group had a 30% higher survival rate than those in the NZBD9 strain-infected group. In addition, the deletion of fliP gene decreased bacterial load in the spleen, intestine, liver as well as head kidney of hybrid grouper and the tissues damage were weakened. Moreover, the infection of hybrid grouper spleen by the Δ fliP strain induced 1,189 differential expression genes compared with the counterpart infected by NZBD9 strain. KEGG enrichment analysis showed that 9 immune-related pathways, 5 signal transduction pathways, and 3 signaling molecules and interaction pathways were significantly enriched. qRT-PCR analysis revealed that the Δ fliP strain mainly up-regulated the expression of inflammation related genes (IL-6 , IL-12 , IL-1β , IL-10 , CXCL8 , CXCL10) and immune regulation related genes (TLR2 , P65 , MyD88 , P85 , AKT), but down-regulated the expression of cell death related genes (FoxO1 , Bim , PLK2 and LDHA) during infection. Based on the above results, fliP gene contributed to the pathogenicity of P. plecoglossicida to hybrid grouper (E. fuscoguttatus ♀ × E. lanceolatus ♂), deletion of fliP gene promoted the inflammation and immune response of hybrid grouper to P. plecoglossicida infection, which accelerating host clearance of pathogen and reducing tissue damages. • fliP is a virulence gene of P. plecoglossicida. • Deletion of the fliP decreased bacterial load and attenuated the tissues damage in grouper. • Deletion of the fliP significantly affected immune response of grouper to P. plecoglossicida infection. [ABSTRACT FROM AUTHOR]