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Background: We described here an aptamer-based magnetic nanoprobe for measuring the amount of chloramphenicol (CAP) in milk. Methods: The nanoprobe presented in this method consists of a magnetic nanoparticle conjugated to a specific CAP aptamer. If the target is detected in the sample, the nanoprobe binds to it, and the aptamer forms a G-quadruplex structure. This structure mimics the peroxidase activity in the presence of the hemin cofactor. If tetramethylbenzidine is added to the sample containing the nanoprobe, a blue color light is observed. After adding a stop reagent solution, the color produced is measured by a microplate reader and a portable meter. Results: This study proves a 99% positive linear relationship between the microplate reader's results and the portable meter results. Conclusion: Conjugation of the aptamer to magnetic nanoparticles and applying magnetic separation operations change the nanoprobe performance by 11% for both mentioned devices. [ABSTRACT FROM AUTHOR]

The application of nanocarriers in medicine and pharmaceuticals suggests a new procedure in nanotechnology terminology, nanomedicine. DNA has been significantly highlighted due to its amazing functionality and nature as a nanomaterial in biomedicine. Given that DNA is biocompatible, its use as a nanomaterial in medicine provides an excellent prospect for the rational engineering of DNA nanostructures. According to new approaches in disease treatment at gene levels, gene therapy, DNA as a nanomedicine plays an essential role in the medical sciences. In this field, researchers have published enormous documents regarding the applications of DNA and DNA-based nanostructures as drug or gene nanocarriers, DNA-based diagnostics, and DNA nano vaccines. In this review, the novelty of DNA-based nanomedicine has been considered. [ABSTRACT FROM AUTHOR]