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Based on the parallel rock samples of tight oil reservoirs, the imbibition comparison experiments respecting to formation water and active water under different experimental conditions have been initiated. With adoption of the experimental measures i.e. Nuclear Magnetic Resonance (NMR), the micro-production mechanism of the enforcement of the imbibition oil recovery effect by the active agent has been analyzed. Surfactant may promote the imbibition effect of samples with different wettability, different water saturation and different sizes. The imbibition recovery ratios of No. 1 hydrophilic core in formation water and active water are respective 15.79% and 17.68%, and the active water imbibition increased by 1.88%, with an enforcement ratio of 11.97%. The imbibition recovery ratios of No. 3 lipophilic core in formation water and active water are respective 10.64% and 13.26%, and the active water imbibition increased by 2.62%, with an enforcement ratio of​ 24.62%. The enforcement effect of surfactant on the lipophilic core is higher than that on the hydrophilic core. The enforcement ratio of No. 1 core in macropores is 8.78%, and that of No. 3 rock is 36.14%, indicating that the surfactant can effectively reduce the seepage resistance of the lipophilic cores and enhance the imbibition recovery ratio of macropores. The increase magnitude and the enforcement ratio of the imbibition recovery of No. 4 low water-contained core are lower than those of No. 6 high water-contained core, indicating that the surfactant has a greater impact on the imbibition of high water-contained cores. The enforcement ratio in active water imbibition in core No. 4 is 28.59% in micropores and 15.46% in macropores; and respective 1460.74% and 9.56% for No. 6 core. The enforcement ratios of micropores in the two cores are both higher than those in macropores. It shows that the surfactant has a great influence on the permeability of micropores in the core. The increment magnitude in active water imbibition of small No. 2 core is 22%, and that of No. 2 large core is 26.93%, indicating that the surfactant has a greater impact on the imbibition of large cores.

How to reduce the damage caused by myocardial ischemia-reperfusion (IR) in a timely manner to save patients' lives is still a great clinical challenge. Although dexmedetomidine (DEX) has been reported to protect the myocardium, the regulatory mechanism of gene translation responding to IR injury and DEX protection is poorly understood. In this study, IR rat model with DEX and the antagonist yohimbine (YOH) pretreatment were established, and RNA sequencing was carried out to seek the important regulators in differential expressed genes. A series of cytokines and chemokine as well as eukaryotic translation elongation factor 1 alpha 2 (EEF1A2) were induced by IR compared to control and compromised by DEX pretreatment compared to IR, then reversed by YOH. Immunoprecipitation was conducted to identify that peroxiredoxin 1 (PRDX1) interacted with EEF1A2 and contributed to the recruitment of EEF1A2 on mRNA molecules of cytokines and chemokine. Knockdown of PRDX1 could weaken the enhancive effect of EEF1A2 for gene translation of IL6, CXCL2 and CXCL11 under the IR condition, and indeed reduce cell apoptosis of cardiomyocytes. We also determined that the RNA motif “USCAGDCU” at 5′ UTR could be particularly recognized by PRDX1. Destruction of this motif at the 5’ UTR of IL6, CXCL2 and CXCL11 by CRISPR-CAS9 could result in the loss occupancies of EEF1A2 and PRDX1 on the mRNA of these three genes. Our observations showed the importance of PRDX1 in the reasonable control of cytokine and chemokine expression to prevent excessive inflammatory response to cell damage.