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IntroductionThis study aims to delineate the etiology and prevalence of isolated pathogens, along with the clinical characteristics of endophthalmitis patients over a 9-year period at hospital in Southwest of China. Additionally, we investigating the metabolic and cellular processes related to environmental factors may offer novel insights into endophthalmitis.MethodsWe analyzed data pertaining to endophthalmitis patients treated at the Affiliated Hospital of Yunnan University from 2015 to 2023. According to our clinical data, we conducted an experiment based on transcriptomics and metabolomics analysis to verify whether environmental factors affect behavior of S. epidermidis by culturating S. epidermidis under oxic and microoxic condition.ResultsIn this study, 2,712 fungi or bacteria strains have been analyzed, gram-positive bacteria constituted 65.08%, with S. epidermidis being the most predominant species (25.55%). Ophthalmic trauma was the primary pathogenic factor for S. epidermidis ocular infections. Regarding fluoroquinolones, S. epidermidis exhibited the higher resistance rate to levofloxacin than moxifloxacin. Moreover, our investigation revealed that S. epidermidis in microoxic environment increase in energy metabolism, amino acid metabolism, and membrane transport.ConclusionOur findings underscore the significance of S. epidermidis as a crucial pathogen responsible for infectious endophthalmitis. It is crucial to exercise vigilance when considering Levofloxacin as the first-line drug for empiric endophthalmitis treatment. The metabolites alteration observed during the commensal-to-pathogen conversion under microoxic condition serve as a pivotal environmental signal contributing to S. epidermidis metabolism remodeling, toward more pathogenic state.

A fictional story is always narrated from a certain narrative voice and mode of focalization. These core narrative techniques have a major impact on how readers interpret the narrative plot and connect with the characters. This study used eye-tracking to investigate how classic narrative reading is affected by narrative voice and focalization. The results showed that the third-person narrative voice was read more slowly than the first-person narrative voice, especially when the narrative was presented with internal focalization. Importantly, the transition from a first-person to a third-person narrative voice generally resulted in longer reading times, whereas a switch from a third-person to a first-person narrative voice only yielded limited benefits in terms of reduced reading time. These findings provide direct evidence to support the assumption that there is a distinction between the first-person narration and the third-person narration and demonstrate the important role of narrative voice and focalization in understanding narrative texts.