48 results on '"Huan, Jia"'
Search Results
2. Decoupling effect and influencing factors of carbon emissions in China: Based on production, consumption, and income responsibilities
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Lu, Zhi-Lin, Wang, Li-Li, Guo, Xue-Peng, Pang, Jun, and Huan, Jia-Jia
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- 2024
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3. Cytochrome c-peroxidase modulates ROS homeostasis to regulate the sexual mating of Sporisorium scitamineum
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Enping Cai, Huan Jia, Ruqing Feng, Wenqiang Zheng, Lei Li, Li Zhang, Zide Jiang, and Changqing Chang
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Sporisorium scitamineum ,cytochrome c-peroxidase ,reactive oxygen species ,pheromone response factor ,mating/filamentation ,Microbiology ,QR1-502 - Abstract
ABSTRACT Sugarcane smut, caused by the basidiomycetous fungus Sporisorium scitamineum, is a global fungal disease resulting in substantial economic losses. Our previous research has highlighted the significant role of reactive oxide species (ROS) in the sexual reproduction of S. scitamineum, yet the underlying mechanisms remain unclear. In this study, we identified the cytochrome c-peroxidase encoding gene, SsCCP1, as crucial for oxidative stress resistance. We further found that absence of SsCcp1 leads to defects in mating/filamentation and disrupts intracellular ROS homeostasis. However, restoration of mating/filamentation of SsCCP1 deletion mutants was partially achieved with exogenous antioxidants (vitamin C or vitamin E). Notably, transcription of the pheromone response factor SsPRF1, pivotal for mating/filamentation of S. scitamineum, is significantly reduced in the SsCCP1 deletion mutants or under exogenous hydrogen peroxide (H2O2) conditions. Nevertheless, the addition of exogenous vitamin C for 60 min significantly up-regulated the SsPRF1 gene in the SsCCP1 deletion mutants. Moreover, the constitutive expression of SsPRF1 essentially reinstated the mating/filamentation of SsCCP1 deletion mutants, suggesting that SsCcp1 mediates the transcriptional activity of SsPRF1 by maintaining ROS homeostasis to regulate the mating/filamentation of S. scitamineum. Further investigations revealed that SsCcp1 is necessary for ROS detoxification and full pathogenicity in planta. Overall, this study not only unveils the importance of SsCcp1 in S. scitamineum mating/filamentation, ROS detoxification, and virulence but also provides insights into the role of SsCcp1-regulated ROS homeostasis in sexual reproduction of S. scitamineum. IMPORTANCE Reactive oxygen species play an important role in pathogen-plant interactions. In fungi, cytochrome c-peroxidase maintains intracellular ROS homeostasis by utilizing H2O2 as an electron acceptor to oxidize ferrocytochrome c, thereby contributing to disease pathogenesis. In this study, our investigation reveals that the cytochrome c-peroxidase encoding gene, SsCCP1, not only plays a key role in resisting H2O2 toxicity but is also essential for the mating/filamentation and pathogenicity of S. scitamineum. We further uncover that SsCcp1 mediates the expression of SsPrf1 by maintaining intracellular ROS homeostasis to regulate S. scitamineum mating/filamentation. Our findings provide novel insights into how cytochrome c-peroxidase regulates sexual reproduction in phytopathogenic fungi, presenting a theoretical foundation for designing new disease control strategies.
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- 2023
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4. Towards a high-intensity muon source
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Han-Jie Cai, Yuan He, Shuhui Liu, Huan Jia, Yuanshuai Qin, Zhijun Wang, Fengfeng Wang, Lixia Zhao, Neng Pu, Jianwei Niu, Liangwen Chen, Zhiyu Sun, Hongwei Zhao, and Wenlong Zhan
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Nuclear and particle physics. Atomic energy. Radioactivity ,QC770-798 - Abstract
A high-intensity muon source driven by a continuous-wave superconducting linac holds the potential to significantly advance the intensity frontier of muon sources. Alongside advancements in accelerator technologies, breakthroughs in muon production target and collection schemes are essential. After a brief introduction to the development of the accelerator-driven system superconducting linac, a novel muon production target is proposed, utilizing a free-surface liquid lithium jet capable of handling the heat power generated by a proton beam with an energy of 600 MeV and a current of 5 mA. It is predicted by our simulation studies that the lithium target is more efficient in surface muon production compared to the rotating graphite target. The parameter space of the front end consisting of a lithium target and a large-aperture capture solenoid is explored, from the perspective of production efficiency, capture efficiency, and characteristics of the surface muon beam.
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- 2024
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5. Thermal study of a scanning beam in granular flow target
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Ping Lin, Yuanshuai Qin, Changwei Hao, Yuan Tian, Jiangfeng Wan, Huan Jia, Lei Yang, Wenshan Duan, Han-Jie Cai, and Sheng Zhang
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Dense granular-flow target ,Scanning beam ,GPU ,Heat deposition ,Heat transfer ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
The concept of dense granular-flow target (DGT) for the China Initiative Accelerator Driven Subcritical system (CiADS) is an attractive choice for high heat removal ability, low chemical toxicity, and radiotoxicity. A wobbling hollow beam is proposed to enhance the homogeneity of temperature rise of flowing particles in beam-target coupling zone. In this paper, the design procedure of target and beam parameters was discussed firstly. Then we simulated the heat deposition and transfer of the scanning beam in DGT to study the effect of beam parameters. The results show the flux density of proton beam plays a crucial role in the distribution of temperature rise while the contributions from scanning frequency heat transfer are also obvious. Moreover, heat transfer in transversal directions is insignificant, resulting in a low heat flux towards the sidewalls of DGT. This work not only contributes to the design of DGT, but also beneficial for understanding the beam-target coupling in porous materials.
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- 2022
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6. Fixed and Sliding FBG Sensors-Based Triaxial Tip Force Sensing for Cable-Driven Continuum Robots.
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Zecai Lin, Huanghua Liu, Xiaojie Ai, Weidong Chen 0001, Anzhu Gao, Zhenglong Sun 0001, Yun Zou, Guang-Zhong Yang, Hao Wu, and Huan Jia
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- 2022
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7. The biomedical knowledge graph of symptom phenotype in coronary artery plaque: machine learning-based analysis of real-world clinical data
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Huan, Jia-Ming, primary, Wang, Xiao-Jie, additional, Li, Yuan, additional, Zhang, Shi-Jun, additional, Hu, Yuan-Long, additional, and Li, Yun-Lun, additional
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- 2024
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8. Beam-target configurations and robustness performance of the tungsten granular flow spallation target for an Accelerator-Driven Sub-critical system
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Han-Jie Cai, Huan Jia, Xin Qi, Ping Lin, Sheng Zhang, Yuan Tian, Yuanshuai Qin, Xunchao Zhang, Lei Yang, and Yuan He
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Granular flow target ,Accelerator-driven sub-critical system ,Beam-target configurations ,Accident conditions ,Robustness performance ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
The dense granular flow spallation target is a new target concept proposed for an Accelerator-Driven Sub-critical (ADS) system. In this paper, the beam-target configurations of a tungsten granular flow target for the ADS with a thermal power of 1 GW is explored. The beam profile options using different scanning methods are discussed. The critical geometry parameters are adjusted to investigate the performance of the granular target from the aspects of neutron efficiency, stability and temperature distribution in target medium. To figure out how the target under accident conditions would behave, different clogging conditions are induced in the simulation. The dynamic processes are analyzed and some important parameters such as abnormal temperature rise and beam cutoff time window are obtained. The response of the sub-critical reactor to a clogging accident is also investigated. It is indicated that the monitoring of the granular flow by the neutron detectors in the sub-critical core will be effective.
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- 2022
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9. Study on Corrosion Resistance of Cationic Waterborne Acrylic Coating
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ZHU Kang, FAN Wan-xin, HUAN Jia-qi, ZHANG Xiao-ya, SUN Ying, HU He-feng
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waterborne anti-corrosive coating ,cationic hydroxy acrylic resin dispersion ,siloxane hydrolyzate ,tertiary amine groups ,corrosion resistance performance ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Technology - Abstract
Aiming at understanding the corrosion resistance of siloxane hydrolyzate cured cationic waterborne acrylic coatings, waterborne metal anti-corrosive coatings were prepared by cationic hydroxy acrylic resin dispersion containing tertiary amine group cured by siloxane hydrolysate WKHY-601.With using DSC, TGA, FT-IR and CHI660E electrochemical workstation, the curing mechanism of the coating and the main factors affecting its salt corrosion resistance were researched systematically.Results showed that the curing of the coating was achieved by the reaction of the silanol hydroxyl group and the alcohol hydroxyl group in the resin and the self-condensation of the siloxane hydrolyzate to form a cross-linked network.The content and type of the tertiary amine group in the resin and the amount of the curing agent siloxane hydrolyzate influenced the salt corrosion resistance of the coating film.As the content of tertiary amine groups increased, the self-corrosion potential of coating increased and the self-corrosion current density of coating declined.After reaching a certain level of the content of tertiary amine groups, the value remained stable.Besides, the dimethyl type tertiary amine group with less steric hindrance was better than the diethyl type, and the increase in the amount of siloxane hydrolyzate was beneficial to improve the compactness of the coating film and enhance its salt corrosion resistance.When the amount of siloxane hydrolyzate was excessive, the content of tertiary amine groups in the coating film would decrease, resulting in weakening the salt corrosion resistance.Furthermore, the self-corrosion potential of the coating sample prepared by DM type resin with a tertiary amine group content of 0.42 mmol/g and siloxane hydrolysate WKHY-601 according to a mass ratio of 3∶1 was as high as-0.298 V, and the self-corrosion current density was only 1.308×10-9 A/cm2, performing excellent salt corrosion resistance.
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- 2022
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10. Genetic findings of Sanger and nanopore single-molecule sequencing in patients with X-linked hearing loss and incomplete partition type III
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Ying Chen, Jiajun Qiu, Yingwei Wu, Huan Jia, Yi Jiang, Mengda Jiang, Zhili Wang, Hai-Bin Sheng, Lingxiang Hu, Zhihua Zhang, Zhaoyan Wang, Yun Li, Zhiwu Huang, and Hao Wu
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IP-III ,POU3F4 ,Nanopore single-molecule sequencing ,Hearing outcomes ,Medicine - Abstract
Abstract Background POU3F4 is the causative gene for X-linked deafness-2 (DFNX2), characterized by incomplete partition type III (IP-III) malformation of the inner ear. The purpose of this study was to investigate the clinical characteristics and molecular findings in IP-III patients by Sanger or nanopore single-molecule sequencing. Methods Diagnosis of IP-III was mainly based on clinical characteristics including radiological and audiological findings. Sanger sequencing of POU3F4 was carried out for these IP-III patients. For those patients with negative results for POU3F4 Sanger sequencing, nanopore long-read single-molecule sequencing was used to identify the possible pathogenic variants. Hearing intervention outcomes of hearing aids (HAs) fitting and cochlear implantation (CI) were also analyzed. Aided pure tone average (PTA) was further compared between two groups of patients according to their different locations of POU3F4 variants: in the exon region or in the upstream region. Results In total, 18 male patients from 14 unrelated families were diagnosed with IP-III. 10 variants were identified in POU3F4 by Sanger sequencing and 6 of these were reported for the first time (p.Gln181*, p.Val215Gly, p.Arg282Gln, p.Gln316*, c.903_912 delins TGCCA and p.Arg205del). Four different deletions that varied from 80 to 486 kb were identified 876–1503 kb upstream of POU3F4 by nanopore long-read single-molecule sequencing. De novo genetic mutations occurred in 21.4% (3/14) of patients with POU3F4 mutations. Among these 18 patients, 7 had bilateral HAs and 10 patients received unilateral CI. The mean aided PTA for HAs and CI users were 41.1 ± 5.18 and 40.3 ± 7.59 dB HL respectively. The mean PTAs for patients with the variants located in the exon and upstream regions were 39.6 ± 6.31 versus 43.0 ± 7.10 dB HL, which presented no significant difference (p = 0.342). Conclusions Among 14 unrelated IP-III patients, 28.6% (4/14) had no definite mutation in exon region of POU3F4. However, possible pathogenic deletions were identified in upstream region of this gene. De novo genetic mutations occurred in 21.4% (3/14) of patients with POU3F4 mutation. There was no significant difference of hearing intervention outcomes between the IP-III patients with variants located in the exon region and in the upstream region.
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- 2022
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11. Preparation and Degradation Performance Study of P(AM/GG/PEGDA) Nanocomposite Self-Degradation Gel Plugging Material
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Dan Bao, Siyuan Liu, Xianli Zhang, Feng Li, Jiaqin Wang, Huan Jia, Shanghao Liu, and Peng Zhang
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reservoir ,drilling fluid lost circulation ,self-degradation plugging material ,gel ,degradation factor ,Science ,Chemistry ,QD1-999 ,Inorganic chemistry ,QD146-197 ,General. Including alchemy ,QD1-65 - Abstract
Lost circulation is a world-class problem, and the contradiction between plugging and unplugging in reservoirs is a problem that needs to be solved urgently. The traditional LCM is not suitable for reservoirs and the complex subsequent operations. Currently, a self-degrading plugging material is proposed. In this paper, a new self-degradation plugging material, CKS-DPPG, was prepared by AM, GG, nano silica, and PEGDA. The effects of reactant concentration, pH, mineralization, etc., on the swelling and degradation performance of CKS-DPPG were investigated. The plugging capacity was tested by fracture plugging equipment, and the mechanism of self-degradation was revealed. The results show that the CKS-DPPG reached a 50% degradation rate in 54 h and complete degradation in 106 h at 80 °C and pH = 8. Low temperatures, high mineralization, and weak alkaline conditions prolong the complete degradation time of CKS-DPPG, which facilitates subsequent operations. The simulation of the 3 mm opening fracture plugging experiment showed that the pressure-bearing capacity reached 6.85 MPa and that a 0.16 MPa pressure difference could unplug after degradation. The ester bond of PEGDA is hydrolyzed under high-temperature conditions, and the spatial three-dimensional structure of CKS-DPPG becomes linear. The CKS-DPPG can effectively reduce subsequent unplugging operations and lower production costs.
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- 2023
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12. A microscope-assisted endoscopic transcanal transpromontorial approach for vestibular schwannoma resection: a preliminary report
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Jianqing, Chen, Yongchuan, Chai, Zhihua, Zhang, Huan, Jia, Zhaoyan, Wang, and Hao, Wu
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- 2022
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13. A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
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Patthara Kongsuphol, Huan Jia, Hoi Lok Cheng, Yue Gu, Bhuvaneshwari D/O Shunmuganathan, Ming Wei Chen, Sing Mei Lim, Say Yong Ng, Paul Ananth Tambyah, Haziq Nasir, Xiaohong Gao, Dousabel Tay, Seunghyeon Kim, Rashi Gupta, Xinlei Qian, Mary M. Kozma, Kiren Purushotorman, Megan E. McBee, Paul A. MacAry, Hadley D. Sikes, and Peter R. Preiser
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Medicine - Abstract
Kongsuphol et al. develop a paper-based, vertical flow assay to detect SARS-CoV-2 neutralizing antibodies. The point-of-care assay has comparable performance to lab-based tests and provides results in 10 min.
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- 2021
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14. Rapid Evaluation of Vaccine Booster Effectiveness against SARS-CoV-2 Variants
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Hoi Lok Cheng, Sing Mei Lim, Huan Jia, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Jyoti Somani, Sharmila Sengupta, Dousabel M. Y. Tay, Patrina W. L. Chua, Abirami R., Sharon Y. H. Ling, Megan E. McBee, Barnaby E. Young, Hadley D. Sikes, and Peter R. Preiser
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COVID ,neutralizing antibodies ,point-of-care test ,Microbiology ,QR1-502 - Abstract
ABSTRACT As the COVID-19 pandemic continues, countries around the world are switching toward vaccinations and boosters to combat the pandemic. However, waning immunity against SARS-CoV-2 wild-type (WT) and variants have been widely reported. Booster vaccinations have shown to be able to increase immunological protection against new variants; however, the protection observed appears to decrease quickly over time suggesting a second booster shot may be appropriate. Moreover, heterogeneity and waning of the immune response at the individual level was observed suggesting a more personalized vaccination approach should be considered. To evaluate such a personalized strategy, it is important to have the ability to rapidly evaluate the level of neutralizing antibody (nAbs) response against variants at the individual level and ideally at a point of care setting. Here, we applied the recently developed cellulose pulled-down virus neutralization test (cpVNT) to rapidly assess individual nAb levels to WT and variants of concerns in response to booster vaccination. Our findings confirmed significant heterogeneity of nAb responses against a panel of SARS-CoV-2 variants, and indicated a strong increase in nAb response against variants of concern (VOCs) upon booster vaccination. For instance, the nAb response against current predominant omicron variant was observed with medians of 88.1% (n = 6, 95% CI = 73.2% to 96.2%) within 1-month postbooster and 70.7% (n = 22, 95% CI = 66.4% to 81.8%) 3 months postbooster. Our data show a point of care (POC) test focusing on nAb response levels against VOCs can guide decisions on the potential need for booster vaccinations at individual level. Importantly, it also suggests the current booster vaccines only give a transient protective response against some VOC and new more targeted formulations of a booster vaccine against specific VOC may need to be developed in the future. IMPORTANCE Vaccination against SARS-CoV-2 induces protection through production of neutralization antibodies (nAb). The level of nAb is a major indicator of immunity against SARS-CoV-2 infection. We developed a rapid point-of-care test that can monitor the nAb level from a drop of finger stick blood. Here, we have implemented the test to monitor individual nAb level against wild-type and variants of SARS-CoV-2 at various time points of vaccination, including post-second-dose vaccination and postbooster vaccination. Huge diversity of nAb levels were observed among individuals as well as increment in nAb levels especially against Omicron variant after booster vaccination. This study evaluated the performance of this point-of-care test for personalized nAb response tracking. It verifies the potential of using a rapid nAb test to guide future vaccination regimens at both the individual and population level.
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- 2022
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15. Finger stick blood test to assess postvaccination SARS‐CoV‐2 neutralizing antibody response against variants
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Sing Mei Lim, Hoi Lok Cheng, Huan Jia, Patthara Kongsuphol, Bhuvaneshwari D/O Shunmuganathan, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Shuvan Prashant Turaga, Sascha P. Heussler, Jyoti Somani, Sharmila Sengupta, Dousabel M. Y. Tay, Megan E. McBee, Barnaby E. Young, Paul A. MacAry, Hadley D. Sikes, and Peter R. Preiser
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cellulose pulldown assay ,COVID19 ,humoral response against COVID19 variants ,neutralizing antibody ,point‐of‐care test ,SARS‐CoV‐2 ,Chemical engineering ,TP155-156 ,Biotechnology ,TP248.13-248.65 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract There is clinical need for a quantifiable point‐of‐care (PoC) SARS‐CoV‐2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi‐quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild‐type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.
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- 2022
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16. Shielding Assessment and Optimization of the Target Station for Medical Isotope Production Based on Superconducting Proton Linac
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Daiyuan Chen, Ricardo dos Santos Augusto, Yuanhong Li, Zhi Qin, Jian Rong, Kaiqiang Yao, Huan Jia, Chenzhang Yuan, Juntao Liu, and Zhiyi Liu
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shielding ,dosimetry ,FLUKA ,medical isotope production ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
In response to the worldwide shortage of 99mTc supply caused by the combined consequence from both decommissioning and maintenance of research reactors worldwide, the Institute of Modern Physics (IMP) of the Chinese Academy of Science and Lanzhou University have launched a collaboration to research and develop a 99mTc production solution based on the 25 MeV high-intensity superconducting proton linear accelerator. Radiation from high-current proton bombardment must be evaluated and considered carefully at the design stage to meet radiation protection (RP) policy and requirements of the shielding for the key device. This work employed FLUKA to conduct the shielding assessment of both prompt and residual radiation fields in several iterations, based on a prototype of the high-power target system. The prompt dose rates outside the target station are lower than the institution’s limit. The residual dose rates inside the station fall below 100 μSv/h at 64 h after the end of beam (EOB); the dominant source term is then the target chamber. The service life of the main actuator is expected to be extended by 2.7 times with the current partial shielding design. The simulation accelerating techniques are applied to balance the accuracy of results and the progress of the project at the same time, which is referential to the shielding assessment of large-scale nuclear facilities. The results can also be used in further study and construction of the target station.
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- 2023
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17. Design and Development of the 200-kW Beam Dump
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Huan Jia, Haihua Niu, Han-Jie Cai, Chenzhang Yuan, Xunchao Zhang, Yuanshuai Qin, Hongming Xie, Baifan Wang, Peng Zhang, Yuxuan Huang, Tieming Zhu, Tianji Peng, Weilong Chen, Qingwei Chu, Jianqiang Wu, Shenghu Zhang, Xiang Li, Duanyang Jia, Bin Zhang, Yuan He, Hongwei Zhao, and Wenlong Zhan
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Nuclear Energy and Engineering - Published
- 2023
18. Engineering thermostable affinity proteins for use in high-throughput immunoassay formats
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Huan Jia, Nazirulmubin Abdul Moomen, Jeanette Leong, Patthara Kongsuphol, Zhi Feng Sherman Lim, Carmen Sze Min Pui, Yuxuan Tan, Ki-Joo Sung, and Hadley D. Sikes
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Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.
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- 2023
19. Simultaneous Bilateral Cochlear Implantation in Very Young Children Improves Adaptability and Social Skills: A Prospective Cohort Study
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Ying, Chen, Yun, Li, Huan, Jia, Wenxi, Gu, Zhaoyan, Wang, Zhihua, Zhang, Minbo, Xue, Jingjie, Li, Wentao, Shi, Linlin, Jiang, Lu, Yang, Olivier, Sterkers, and Hao, Wu
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Speech and Hearing ,Otorhinolaryngology - Abstract
To investigate the value of using the Gesell Development Diagnosis Scale (GDDS) to predict developmental outcomes in very young children who undergo simultaneous bilateral cochlear implantation.In this prospective cohort study, a repeated-measures investigation was conducted in a tertiary referral hospital. A total of 62 children receiving simultaneous bilateral cochlear implantations were enrolled from April 2017 to August 2018. They were divided into 2 groups depending on the operative age: "Infants" group (6 to 12 months, N = 38) or "Children" group (12 to 36 months, N = 24). Data on the surgical outcomes, auditory development, speech production, and developmental indicators were collected until 2 years after the initial fitting. The primary outcome measure was the GDDS, a neuropsychological development examination. Secondary outcomes included the following: complication rate, aided pure-tone average, Infant-Toddler Meaningful Auditory Integration Scale, Categories of Auditory Performance-II, Meaningful Use of Speech Scale, Speech Intelligibility Rating, and the LittlEARS Auditory Questionnaire.The mean ages at implantation in infants and children groups were 9.2 ± 1.17 and 16.6 ± 3.60 months, respectively. Significant differences were found in the social skills (p = 0.001) and adaptability (p = 0.031) domains of GDDS. The younger the age of bilateral cochlear implants surgery, the higher developmental quotient of language, social skills, and adaptability the child could achieve after 2 years. The complication rates in the infants and children groups were 0% versus 2.1% (p = 0.57). There was no surgical complication in the infants group. In the children group, 1 case with enlarged vestibular aqueduct and Mondini malformation had a receiver-implant misplacement on the right side (2%, 1/48). In the two groups, auditory performance and speech production had improved similarly. In the infants group, social skills developmental quotient at baseline had a significant positive relationship with Meaningful Use of Speech Scale after 2 years.Simultaneous bilateral cochlear implantation in younger children improves adaptability and social skills. GDDS is a sensitive tool of evaluating short-term effect of bilateral cochlear implants in neuropsychological development and constitutes a reliable predictor of speech production for the very younger pediatric cochlear implant users.
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- 2022
20. Novel Molecular-Level Insight into the Self-Healing Behavior and Mechanism of Polyurethane-Urea Elastomer Based on a Noncovalent Strategy
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Jialiang Chen, Chichao Li, Huan Jia, Zhihua Shen, Rong Zhao, Tao Su, Bo Xiang, Xiujuan Wang, Danil W. Boukhvalov, Zhenyang Luo, and Yanlong Luo
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Inorganic Chemistry ,Polymers and Plastics ,Organic Chemistry ,Materials Chemistry - Published
- 2022
21. A Decreased Absolute Number of Treg Cells in Patients with Active Rheumatoid Arthritis is Associated with Elevated Serum Osteopontin Levels with Disease Progression
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Jian-Fang Xie, Jia Wang, Huan-Huan Bai, Jiao-Jiao He, Rui-Huan Jia, Xia Wang, Wen-Qi Zhang, Xiang-Cong Zhao, Xian-Cheng Zhang, Guang-Ying Liu, and Xiao-Feng Li
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Pharmacology (medical) ,General Medicine - Published
- 2022
22. Effect of botanical drugs in improving symptoms of hypertensive nephropathy: Analysis of real-world data, retrospective cohort, network, and experimental assessment
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Huan, Jia-Ming, primary, Ma, Xi-Ting, additional, Li, Si-Yi, additional, Hu, Dong-Qing, additional, Chen, Hao-Yu, additional, Wang, Yi-Min, additional, Su, Xiao-Yi, additional, Su, Wen-Ge, additional, and Wang, Yi-Fei, additional
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- 2023
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23. Analysis of the Real-World Application Laws for Zhengan Xifeng Decoction in the Treatment of Hypertension Based on Machine Learning
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Huan, Jia-Ming, primary, Cai, Dan-Yang, additional, Li, Jie, additional, Hua, Zhen, additional, Chen, Xiao-Qing, additional, Yang, Wen-Qing, additional, Hu, Yuan-Long, additional, Wang, Yi-Fei, additional, and Li, Yun-Lun, additional
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- 2023
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24. Association between low blood selenium concentrations and poor hand grip strength in United States adults participating in NHANES (2011–2014)
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Jingjing Pei, Lailai Yan, Yuwan Wu, Xi Zhang, Huan Jia, and Huajun Li
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Nutrition and Dietetics ,Physiology ,Physiology (medical) ,Endocrinology, Diabetes and Metabolism ,General Medicine - Abstract
The trace element selenium, which is found in selenoproteins, plays an antioxidant role in preventing muscle tissue injury. A positive association between selenium concentrations and hand grip strength has been reported in older adults; however, the evidence of this association is scarce in general adults. In this study, we aimed to evaluate the association between blood selenium concentrations and low hand grip strength using the data from the National Health and Nutrition Examination Survey 2011–2012 and 2013–2014 in the United States (US). Logistic regression was used to calculate the odds ratio (OR) of low hand grip strength, with blood selenium level adjusted for potential confounders. Among 8158 adults (women: 51.59%) with a mean age of 47 (range: 18–80) years, women and non-Hispanic Blacks tended to have low blood selenium concentrations. Notably, participants with high blood selenium concentrations (range, 178.1–192.5 µg/L) were more likely to have a low risk of low hand grip strength after adjusting for the potential covariates (OR: 0.60, 95% confidence interval (CI): 0.38–0.95) than those with low blood selenium concentrations. After excluding participants with chronic diseases, high blood selenium concentrations were found to be associated with a low risk of low hand grip strength (OR: 0.30, 95% CI: 0.14–0.65). A J-shaped relationship was found between selenium concentrations and low hand grip strength ( P for nonlinear trend
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- 2023
25. Data from SET Domain–Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence
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Wei-Jun Yang, Yi-Ding Chen, Jiao-Jiao Zhou, Yue-Hong Wang, Guo-Ping Ding, Sheng-Nan Jia, Jin-Shu Yang, Xue-Ting Huang, Qian-Yun Lu, Yao-Shun Yang, Sun-Li Cai, Qian Zhu, Jing-Yi Feng, Xiao-Li Liu, Wen-Huan Jia, Yan-Fu Ding, and Sen Ye
- Abstract
Quiescent cancer stem cells (CSC) play important roles in tumorigenesis, relapse, and resistance to chemoradiotherapy. However, the determinants of CSC quiescence and how they sustain themselves to generate tumors and relapse beyond resistance to chemoradiotherapy remains unclear. Here, we found that SET domain–containing protein 4 (SETD4) epigenetically controls breast CSC (BCSC) quiescence by facilitating heterochromatin formation via H4K20me3 catalysis. H4K20me3 localized to the promoter regions and regulated the expression of a set of genes in quiescent BCSCs (qBCSC). SETD4-defined qBCSCs were resistant to chemoradiotherapy and promoted tumor relapse in a mouse model. Upon activation, a SETD4-defined qBCSC sustained itself in a quiescent state by asymmetric division and concurrently produced an active daughter cell that proliferated to produce a cancer cell population. Single-cell sequence analysis indicated that SETD4+ qBCSCs clustered together as a distinct cell type within the heterogeneous BCSC population. SETD4-defined quiescent CSCs were present in multiple cancer types including gastric, cervical, ovarian, liver, and lung cancers and were resistant to chemotherapy. SETD4-defined qBCSCs had a high tumorigenesis potential and correlated with malignancy and chemotherapy resistance in clinical breast cancer patients. Taken together, the results from our previous study and current study on six cancer types reveal an evolutionarily conserved mechanism of cellular quiescence epigenetically controlled by SETD4. Our findings provide insights into the mechanism of tumorigenesis and relapse promoted by SETD4-defined quiescent CSCs and have broad implications for clinical therapies.Significance:These findings advance our knowledge on the epigenetic determinants of quiescence in cancer stem cell populations and pave the way for future pharmacologic developments aimed at targeting drug-resistant quiescent stem cells.
- Published
- 2023
26. Supplementary Data from SET Domain–Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence
- Author
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Wei-Jun Yang, Yi-Ding Chen, Jiao-Jiao Zhou, Yue-Hong Wang, Guo-Ping Ding, Sheng-Nan Jia, Jin-Shu Yang, Xue-Ting Huang, Qian-Yun Lu, Yao-Shun Yang, Sun-Li Cai, Qian Zhu, Jing-Yi Feng, Xiao-Li Liu, Wen-Huan Jia, Yan-Fu Ding, and Sen Ye
- Abstract
Supplementary Table S1 and Supplementary Figure S1 to S11
- Published
- 2023
27. Thermal-Mechanical Analysis of Beam Window and Beam Tube for ADS Granular Flow Target
- Author
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Weiping Deng, Yanbin Zhang, Huan Jia, Tao Wan, Weifeng Yang, Chengwen Qiang, Long Li, Fei Wang, Honglin Ge, Fei Ma, and Xueying Zhang
- Subjects
Nuclear Energy and Engineering - Published
- 2022
28. Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance
- Author
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Huan Jia, Eric A. Miller, Chia Ching Chan, Say Yong Ng, Mookkan Prabakaran, Meng Tao, Ian Shen-Yi Cheong, Sing Mei Lim, Ming Wei Chen, Xiaohong Gao, Abirami R., Megan E. McBee, Peter R. Preiser, Hadley D. Sikes, and Patthara Kongsuphol
- Subjects
body regions ,SARS-CoV-2 ,Point-of-Care Systems ,Biomedical Engineering ,COVID-19 ,Humans ,Bioengineering ,General Chemistry ,Pandemics ,Sensitivity and Specificity ,Biochemistry - Abstract
Surveillance of SARS-CoV-2 infection is critical for controlling the current pandemic. Antigen rapid tests (ARTs) provide a means for surveillance. Available lateral flow assay format ARTs rely heavily on nitrocellulose paper, raising challenges in supply shortage. Vertical flow assay (VFA) with cellulose paper as test material attracts much attention as a complementary test approach. However, current reported VFAs are facing challenges in reading the test signal from the bottom face of the test cassette, complicating the test workflow and hindering translation into rapid test application. Here, we address this gap with an enhanced VFA against SARS-CoV-2 N protein that adapts a cellulose pull-down test format allowing (1) one-step sample application at the top of the test cassette and (2) readout of the test signal from the top. We also demonstrate the feasibility of translating the enhanced VFA into a point-of-care application that can help in SARS-CoV-2 surveillance.
- Published
- 2022
29. Origins of biallelic inactivation of NF2 in neurofibromatosis type 2
- Author
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Lu Xue, Weiwei He, Yi Zhang, Zhigang Wang, Hongsai Chen, Zhe Chen, Weidong Zhu, Dongmei Liu, Huan Jia, Yi Jiang, Zhaoyan Wang, and Hao Wu
- Subjects
Neurofibromatosis 2 ,Neurofibromin 2 ,Cancer Research ,Phenotype ,Oncology ,Mosaicism ,Genes, Neurofibromatosis 2 ,Basic and Translational Investigations ,Mutation ,otorhinolaryngologic diseases ,High-Throughput Nucleotide Sequencing ,Humans ,Neurology (clinical) - Abstract
Background Elucidating the mechanism by which biallelic inactivation evolved could provide a mechanistic understanding for NF2 tumorigenesis and also a rationale for clinical management. Methods A cohort of 60 NF2 patients was recruited. Next-generation sequencing of tumor and paired control samples was used to explore how NF2 mutations evolve in determining the clinical phenotypes. Results In total, 60 blood samples (one from each patient) and 61 (from 35 patients) NF2-associated tumors were collected. Next-generation sequencing of the blood samples detected “first hit” NF2 mutation in 35/60 donors (58.3%), 82.9% of which (29/35) bear heterozygous germline mutations, and 17.1% (6/35) of which are mosaics with variable allelic frequency (VAF). While a number of NF2 patients were found without germline mutation, most (57/61, 93.4%) NF2-associated tumors were identified with NF2 somatic mutation. We calculated the correlation between the onset latency of mosaic and germline NF2 allele carriers with the mosaicism VAF. The mosaicism VAF is negatively and linearly correlated to clinical symptom onset latency (R2 = 0.3677, P = .00351), suggesting biallelic inactivation probability is a linear function of “first hit” prevalence in the body. The second NF2 somatic mutation occurrence time positively correlates with the onset of clinical symptoms (R2 = 0.4151, P = .02633), suggesting tumor growth is linearly proportional to the time after biallelic inactivation. Conclusions Our results suggested that biallelic inactivation of NF2 evolved through neutral drift and preexisting first hit NF2 allele determines certain aspects of the clinical symptoms. Genetic diagnosis should be included in the diagnostic criteria and treatment consideration of NF2.
- Published
- 2021
30. Accelerating the optimization of vertical flow assay performance guided by a rational systematic model-based approach
- Author
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Dousabel M.Y. Tay, Seunghyeon Kim, Yining Hao, Emma H. Yee, Huan Jia, Sydney M. Vleck, Makaya Chilekwa, Joel Voldman, and Hadley D. Sikes
- Subjects
History ,Polymers and Plastics ,Electrochemistry ,Biomedical Engineering ,Biophysics ,General Medicine ,Business and International Management ,Industrial and Manufacturing Engineering ,Biotechnology - Abstract
Rapid diagnostic tests (RDTs) have shown to be instrumental in healthcare and disease control. However, they have been plagued by many inefficiencies in the laborious empirical development and optimization process for the attainment of clinically relevant sensitivity. While various studies have sought to model paper-based RDTs, most have relied on continuum-based models that are not necessarily applicable to all operation regimes, and have solely focused on predicting the specific interactions between the antigen and binders. It is also unclear how the model predictions may be utilized for optimizing assay performance. Here, we propose a streamlined and simplified model-based framework, only relying on calibration with a minimal experimental dataset, for the acceleration of assay optimization. We show that our models are capable of recapitulating experimental data across different formats and antigen-binder-matrix combinations. By predicting signals due to both specific and background interactions, our facile approach enables the estimation of several pertinent assay performance metrics such as limit-of-detection, sensitivity, signal-to-noise ratio and difference. We believe that our proposed workflow would be a valuable addition to the toolset of any assay developer, regardless of the amount of resources they have in their arsenal, and aid assay optimization at any stage in their assay development process.
- Published
- 2022
31. A Rapid Temporal Bone Localization Method Based on Machine Visual Detection Markers
- Author
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Jian-jun, Li, primary, Jian-ye, Zhuo, additional, Yue, Wu, additional, Zuo, Wang, additional, Zhen, Yao, additional, Huan, Jia, additional, Zhao-yan, Wang, additional, Ying, Chen, additional, Dao-min, Zhou, additional, and Ming-zhou, Yu, additional
- Published
- 2022
- Full Text
- View/download PDF
32. Strategies of eliminating nuclear waste using accelerator-driven system in the transition stage for sustainable and clean nuclear energy in China
- Author
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Xunchao Zhang, Neng Pu, Hanjie Cai, Huan Jia, and Yuan He
- Subjects
Nuclear Energy and Engineering - Published
- 2023
33. Four peptidoglycan recognition proteins are indispensable for antibacterial immunity in the cigarette beetle Lasioderma serricorne (Fabricius)
- Author
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Yi Yan, Ming-Huan Jia, Zhi-Jun Le, Kang-Kang Xu, Can Li, and Wen-Jia Yang
- Subjects
Staphylococcus aureus ,General Medicine ,Peptidoglycan ,Biochemistry ,Adenosine Monophosphate ,Immunity, Innate ,Anti-Bacterial Agents ,Coleoptera ,Structural Biology ,Larva ,Receptors, Pattern Recognition ,Escherichia coli ,Animals ,Amino Acids ,Carrier Proteins ,Molecular Biology - Abstract
The peptidoglycan recognition protein (PGRP), an important pattern recognition receptor of insects, is significant for reducing innate immunity and effective pest control. We cloned four PGRP genes (LsPGRP-LB, LsPGRP-LB1, LsPGRP-LE, and LsPGRP-SC2) from the cigarette beetle, Lasioderma serricorne (Fabricius), which encoded proteins of 216, 197, 317, and 190 amino acids, respectively. Three LsPGRPs were predominantly expressed in the larval and pupal stages, whereas LsPGRP-LE displayed high expression in adults. All the four LsPGRPs genes were highly expressed in the midgut and integument. Pathogen inoculation revealed that the four LsPGRPs actively responded to Escherichia coli and its peptidoglycan. The transcription levels of LsPGRP-LE and LsPGRP-SC2 increased significantly after Staphylococcus aureus stimulation. RNA interference-mediated knockdown of the four LsPGRPs led to increased larval mortality when challenged by E. coli, and the expression of four antimicrobial peptide genes (LsCole, LsAtt2, LsDef1 and LsDef2) had a significant decrease. Higher mortality and lower AMP expression were also observed in L. serricorne under S. aureus infection after silencing LsPGRP-LE and LsPGRP-SC2. Our results suggest that the four LsPGRP genes play important and distinct regulatory roles in the antibacterial defense response of L. serricorne.
- Published
- 2022
34. Development and cold-test of an RFQ-DTL coupled cavity
- Author
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Wei-Ping Dou, Chen-Xing Li, Zhi-Jun Wang, Zong-Heng Xue, Xiao-Feng Jin, Zhi-Hui Li, Xiao-Xiao Li, Chao Wang, Wang-Sheng Wang, Huan Jia, Wei-Long Chen, Zhou-Li Zhang, Yuan-Shuai Qin, Xin Qi, and Yuan He
- Subjects
Nuclear and High Energy Physics ,Astronomy and Astrophysics ,Atomic and Molecular Physics, and Optics - Abstract
With the continuous strengthening of application requirements, neutron sources are becoming more and more compact, integrated and mobile. There are challenges in two aspects. The first is to achieve high transmission efficiency of high beam current physically, the second is to achieve integration and miniaturization technically. The coupling of RFQ and DTL is a feasible way. A preliminary study on the key physical problems in the RFQ-DTL coupled cavity has been accomplished. For the beam dynamics, the compact matching of RFQ and DTL was studied for the coupled cavity. Taking the compact physical design of the RFQ-DTL for deuterium ion beam of 30 mA with the energy of 11 MeV without MEBT as an example, we studied the smooth transition of the phase advance per unit length, the ability of RFQ transition cell to rotate beam in the transverse phase space, the transverse and longitudinal acceptance optimization of DTL. The RFQ and DTL can be matched transversely and longitudinally after removing MEBT, and the transmission efficiency is 98.7%. For direct coupling of four-vane RFQ and CH-DTL, we studied the realization of the coupling, the factors affecting the field amplitude ratio, the tuning of the cavity, and the influence of the superimposed field on the beam transmission. The field amplitude ratio between RFQ and CH-DTL is from 16.5 to 36.2. Under the influence of the superimposed field, the difference in normalized RMS emittance is less than 1%. We have designed, constructed and cold-test the 750 MHz model cavity. The normalized electric field coefficients measured and simulated are basically in agreement.
- Published
- 2022
35. Blood glucose mediated the effects of cognitive function impairment related to aluminum exposure in Chinese aluminum smelting workers
- Author
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Xu, Shi-meng, primary, Pan, Bao-long, additional, Gao, Dan, additional, Zhang, Yun-wei, additional, Huan, Jia-ping, additional, Han, Xiao, additional, Song, Jing, additional, Wang, Lin-ping, additional, Zhang, Hui-fang, additional, Niu, Qiao, additional, and Lu, Xiao-ting, additional
- Published
- 2022
- Full Text
- View/download PDF
36. Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays
- Author
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Elliot, Corless, Yining, Hao, Huan, Jia, Patthara, Kongsuphol, Dousabel M Y, Tay, Say Yong, Ng, and Hadley D, Sikes
- Subjects
Immunoassay ,Epitopes ,Antibodies, Monoclonal ,Gene Library - Abstract
Many point-of-care diagnostic tests rely on a pair of monoclonal antibodies that bind to two distinct epitopes of a molecule of interest. This protocol describes the identification and generation of such affinity pairs based on an easily produced small protein scaffold rcSso7d which can substitute monoclonal antibodies. These strong binding variants are identified from a large yeast display library. The approach described can be significantly faster than antibody generation and epitope binning, yielding affinity pairs synthesized in common bacterial protein synthesis strains, enabling the rapid generation of novel diagnostic tools.
- Published
- 2022
37. Candidacy for Cochlear Implantation in Prelingual Profoundly Deaf Adult Patients
- Author
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Ghizlene Lahlou, Hannah Daoudi, Evelyne Ferrary, Huan Jia, Marion De Bergh, Yann Nguyen, Olivier Sterkers, and Isabelle Mosnier
- Subjects
cochlear implant ,prelingual profound hearing loss ,speech perception ,quality of life ,otorhinolaryngologic diseases ,General Medicine - Abstract
Cochlear implantation is usually not recommended for prelingual profoundly deaf adults, although some of these patients might benefit from it. This study aims to define the candidates for cochlear implantation in this population. This retrospective study reviewed 34 prelingual profoundly deaf patients who had received a cochlear implant at 32 ± 1.7 years old (16–55), with at least 1 year of follow-up. Speech perception and quality of life were assessed before and 3, 6, and 12 months after cochlear implantation, then every year thereafter. According to the word speech intelligibility in quiet (WSI) 1 year after implantation, two groups were identified: good performer (GP) with WSI ≥ 50% (n = 15), and poor performer (PP) with WSI ≤ 40% (n = 19). At the 1 year mark, mean WSI improved by 28 ± 4.6% (−20–100) (p < 0.0001). In GP, the intelligibility for words and sentences, communication and quality of life scales improved. In PP, the communication scale improved, but not auditory performance or quality of life. GP and PP differed pre-operatively in speech production, communication abilities, and WSI in best-aided conditions. In prelingual profoundly deaf adults, a dramatic auditory performance benefit could be expected after cochlear implantation if the patients have some degree of speech intelligibility in aided conditions and have developed oral communication and speech production.
- Published
- 2022
- Full Text
- View/download PDF
38. A Review on Solid-State Li-S Battery: From the Conversion Mechanism of Sulfur to Engineering Design.
- Author
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Huan-Huan Jia, Chen-Ji Hu, Yi-Xiao Zhang, and Li-Wei Chen
- Subjects
LITHIUM sulfur batteries ,ENGINEERING design ,ENERGY density ,SOLID electrolytes ,CATHODES - Abstract
Copyright of Journal of Electrochemistry is the property of Journal of Electrochemistry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
39. Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays
- Author
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Elliot Corless, Yining Hao, Huan Jia, Patthara Kongsuphol, Dousabel M. Y. Tay, Say Yong Ng, and Hadley D. Sikes
- Published
- 2022
40. Evaluation of OpenMC calculations coupling with PHITS, FLUKA, and GEANT4 for ADS study
- Author
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Neng Pu, Xun-Chao Zhang, Han-Jie Cai, Huan Jia, Tian-Jiao Liang, and Yuan He
- Subjects
Nuclear Energy and Engineering ,Energy Engineering and Power Technology ,Safety, Risk, Reliability and Quality ,Waste Management and Disposal - Published
- 2023
41. A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
- Author
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Hoi Lok Cheng, Peter R. Preiser, O Shunmuganathan, Hadley D. Sikes, Haziq Nasir, Patthara Kongsuphol, Mary M Kozma, Paul Ananth Tambyah, Bhuvaneshwari D, Megan E. McBee, Seunghyeon Kim, Xiaohong Gao, Say Yong Ng, Paul A. MacAry, Dousabel M Y Tay, Kiren Purushotorman, Rashi Gupta, Sing Mei Lim, Huan Jia, Xinlei Qian, Yue Gu, and Ming Wei Chen
- Subjects
biology ,Immunity ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,biology.protein ,Medicine ,Antibody ,Vaccine efficacy ,Cellulose binding ,Virology ,Virus ,Herd immunity ,Point of care - Abstract
Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor—RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens. In response to infections, the human body produces proteins called antibodies. Neutralizing antibodies (NAbs) are one type of such proteins that are capable of inactivating the target, such as the SARS-CoV-2 virus that causes COVID-19. Monitoring levels of NAb allows us to understand levels of protective immunity. However, current methods to measure NAb are laboratory-based and are not necessarily suitable for large scale NAb monitoring in a large population. We develop a rapid test to detect SARS-CoV-2 NAb in 10 min that can be operated outside a laboratory. Our test provides results that are comparable to lab-based tests, which require between 1 h and up to 2 days to get a result. Our test may be useful for large-scale monitoring of immunity, for example in populations that do not have routine access to a lab. Kongsuphol et al. develop a paper-based, vertical flow assay to detect SARS-CoV-2 neutralizing antibodies. The point-of-care assay has comparable performance to lab-based tests and provides results in 10 min.
- Published
- 2021
42. Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants
- Author
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Sing Mei Lim, Hoi Lok Cheng, Huan Jia, Patthara Kongsuphol, Bhuvaneshwari D/O Shunmuganathan, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Shuvan Prashant Turaga, Sascha P. Heussler, Jyoti Somani, Sharmila Sengupta, Dousabel M. Y. Tay, Megan E. McBee, Barnaby E. Young, Paul A. MacAry, Hadley D. Sikes, Peter R. Preiser, School of Biological Sciences, Lee Kong Chian School of Medicine (LKCMedicine), Singapore-MIT Alliance in Research and Technology (SMART), National Centre for Infectious Diseases, and Tan Tock Seng Hospital
- Subjects
COVID19 ,Biomedical Engineering ,Pharmaceutical Science ,Medicine [Science] ,Cellulose Pulldown Assay ,Biotechnology - Abstract
There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population. Ministry of Health (MOH) National Medical Research Council (NMRC) National Research Foundation (NRF) Published version This study is supported by National Health Innovation Singapore (NHIC) grant # NHIC-COVID19-2005004, National Research Foundation via CREATE Share grant #R571-002-021-592 and the Anti-microbial Resistance Interdisciplinary Research Group (AMR-IRG) of Singapore-MIT Alliance in Research and Technology (SMART). All samples acquired from National Centre for Infectious Diseases (NCID) were supported by Singapore Ministry of Health's National Medical Research Council COVID-19 Research Fund: COVID19RF-0008.
- Published
- 2021
43. Predicting Coupled Herbs for the Treatment of Hypertension Complicated with Coronary Heart Disease in Real-World Data Based on a Complex Network and Machine Learning
- Author
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Huan, Jia-Ming, primary, Li, Yun-Lun, additional, Zhang, Xin, additional, Wei, Jian-Liang, additional, Peng, Wei, additional, Wang, Yi-Min, additional, Su, Xiao-Yi, additional, Wang, Yi-Fei, additional, and Su, Wen-Ge, additional
- Published
- 2022
- Full Text
- View/download PDF
44. Characterization, signal estimation and analyzing of cold button BPMs for a low-β helium/proton superconducting LINAC
- Author
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Yong Zhang, Xuejing Hu, Huan Jia, Hongming Xie, Zhixue Li, Shuhui Liu, Guangyu Zhu, Junxia Wu, Ze Du, Zhaolong Zhang, and Fafu Ni
- Subjects
Nuclear and High Energy Physics ,Instrumentation - Published
- 2022
45. Predicting the Radiated Noise of a Submarine Propeller with Different Types of Control Surfaces.
- Author
-
Jui-Hsiang Kao, Shang-Sheng Chin, Fang-Nan Chang, Yu-Han Tsai, Hua-Tung Wu, and Huan-Jia Xu
- Subjects
PROPELLERS ,COMPUTATIONAL fluid dynamics ,SUBMARINES (Ships) ,DEEP diving ,CAVITATION ,NOISE - Abstract
The objective of this paper is to predict the noise radiated from submarine propellers with different control surface types (the cross- and X-type). When the propellers are free from cavitation, such as those of submarines at a diving depth, the radiated noise dominate, due to unsteady propeller forces. A well-known submarine model (DARPA SUBOFF) is taken as the computing sample. Simulations for hydrodynamics, including stern wakes and unsteady propeller forces, are carried out by using CFD (Computational Fluid Dynamics) technology, and the results are compared with the experimental data. The accuracy of the predicted noise depends on the CFD results. Comparisons between the CFD results and the experimental data are in good agreement. The CFD results are treated as dipole strengths in the linear wave theory to predict the radiated noise caused by the unsteady forces of the propeller. It is found that, when the control surface is of the X-type, the propeller inflow is more uniform, and the radiated noise can be decreased by about 5 dB compared to the cruciform control surface. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
46. ProfKin: A comprehensive web server for structure-based kinase profiling
- Author
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Shuo Yang, Yuan Yuan, Guo-Bo Li, Honglin Li, Zhiqiang Qiu, Yu-Hang Yan, Ruiqiong Wang, Zihao Shen, Huan Jia, and Sang Zhu
- Subjects
Pharmacology ,Profiling (computer programming) ,Web server ,Subfamily ,Dose-Response Relationship, Drug ,Molecular Structure ,Databases, Pharmaceutical ,Drug discovery ,Chemistry ,Kinase ,Organic Chemistry ,General Medicine ,Computational biology ,Ligands ,Ligand (biochemistry) ,computer.software_genre ,Structure-Activity Relationship ,Drug Discovery ,Humans ,Structure based ,Binding site ,Protein Kinase Inhibitors ,Protein Kinases ,computer ,Software - Abstract
Protein kinases are central mediators of signal-transduction cascades and attractive drug targets for therapeutic intervention. Since kinases are structurally and mechanistically related to each other, kinase inhibitor selectivity is often investigated by kinase profiling and considered as an important index for drug discovery. We here describe a versatile web server termed ProfKin for structure-based kinase profiling, which is based on a kinase-ligand focused database (KinLigDB). It provides all ready-to-use 3D structure coordinates of 4219 kinase-ligand complex structures covering 297 human kinases and the associated information, particularly including binding site type, binding ligand type, interaction fingerprints, downstream molecules and related human diseases. The web server works via predicting possible binding modes for the query molecule, prioritizing the binding modes guided by an interaction fingerprint analysis method, and giving a list of ranked kinases by a comprehensive index. Users can freely select entire or part of the KinLigDB database, e.g. via subfamily and binding site type, to customize the profiling contents. The superimpositions of the predicted binding poses of the query molecule with reference binding modes can be visually inspected on the website. The additional classification attributes and phylogenetic tree are also given for each top-ranked kinase.
- Published
- 2021
47. Understanding the Self‐Healing Mechanism of Polyurethane Elastomer Based on Hydrogen Bonding Interactions through Molecular Dynamics Simulation
- Author
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Huan Jia, Yanlong Luo, Meng Zhang, Yangyang Gao, Xianling Chen, Zhenyang Luo, and Zhihua Shen
- Subjects
Materials science ,Polymers and Plastics ,Hydrogen bond ,Organic Chemistry ,Condensed Matter Physics ,Polyurethane elastomer ,Inorganic Chemistry ,Molecular dynamics ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Self-healing ,Materials Chemistry ,Mechanism (sociology) ,Polyurethane - Published
- 2021
48. [Pollution Characteristics, Source Apportionment, and Meteorological Response of Water-soluble Ions in PM 2.5 in Xinxiang, North China].
- Author
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Liu HJ, Li LQ, Li HL, Ren Y, Xu MY, Jia MK, Liu HZ, Yang Y, Song TS, and Hong QH
- Abstract
Pollution variation, source characteristics, and meteorological effects of water-soluble inorganic ions (WSIIs) in PM
2.5 were analyzed in Xinxiang city, Henan Province. PM2.5 samples and their chemical components were monitored online by using URG-9000 in four seasons:winter (January, 2022), spring (April, 2022), summer (July, 2022), and fall (October, 2022). The results showed that the TWSIIs had the same seasonal fluctuations as PM2.5 . The average seasonal concentrations of WSIIs ranged from 19.62-72.15 μg·m-3 , accounting for more than 60% of PM2.5 , demonstrating that WSIIs were the major components of PM2.5 . The annual concentration value of NO3 - /SO4 2- was 2.11, which showed an increasing trend, suggesting predominantly mobile sources for secondary inorganic aerosols (SNA). Further, the molar concentration value [NH4 + ]/[NO3 - ] was 1.95, demonstrating that agriculture emissions were the dominant contributors to atmospheric nitrogen. Furthermore, the backward trajectory analysis showed that the concentrations of Ca2+ and Mg2+ were higher when the northeasterly wind prevailed and the wind speed was high. High values of SOR and NOR were correlated with low temperatures and high relative humidity ( T < 8℃, RH > 60%), demonstrating that more gaseous precursors were converted into sulfate and nitrate. At high temperatures ( T > 24℃), there was no apparent high NOR value like that for SOR, mainly due to the decomposition of NH4 NO3 at high temperatures. Finally, backward trajectories associated with the PMF-resolved results were used to explore the regional transport characteristics. The results illustrated that dust sources in the study areas were mainly influenced by air trajectories originating from the northwest regions, whereas secondary sulfate, secondary nitrate, and biomass sources contributed more to WSIIs when wind speed and altitude air masses were low in the area surrounding the observation site.- Published
- 2024
- Full Text
- View/download PDF
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