Search

Background: Single‐cell RNA sequencing analysis has been usually conducted on post‐traumatic epilepsy (PET) and hereditary epilepsy (HE) patients; however, the transcriptome of patients with traumatic temporal lobe epilepsy has rarely been studied. Materials and Methods: Hippocampus tissues isolated from one patient with PTE and one patient with HE were used in the present study. Single cell isolates were prepared and captured using a 10× Genomics Chromium Single‐Cell 3′ kit (V3) according to the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq 6000 sequencing system. Raw data were processed, and the cells were filtered and classified using the Seurat R package. Uniform Manifold Approximation and Projection was used for visualization. Differentially expressed genes (DEGs) were identified based on a p‐value ≤0.01 and log fold change (FC) ≥0.25. Gene Ontology (GO, http://geneontology.org/) and KEGG (Kyoto Encyclopedia of Genes and Genomes, www.genome.jp/kegg) analyses were performed on the DEGs for enrichment analysis. Results: The reads obtained from the 10× genomic platform for PTE and HE were 39.56 M and 30.08 M, respectively. The Q30 score of the RNA reads was >91.6%. After filtering, 7479 PTE cells and 9357 HE cells remained for further study. More than 96.4% of the reads were mapped to GRCh38/GRCm38. The cells were differentially distributed in two groups, with higher numbers of oligodendrocytes (6522 vs. 2532) and astrocytes (133 vs. 52), and lower numbers of microglial cells (2242 vs. 3811), and neurons (3 vs. 203) present in the HE group than in the PTE group. The DEGs in four cell clusters were identified, with 25 being in oligodendrocytes (13 upregulated and 12 downregulated), 87 in microglia cells (42 upregulated and 45 downregulated), 222 in astrocytes (115 upregulated and 107 downregulated), and 393 in neurons (305 upregulated and 88 downregulated). The genes MTND1P23 (downregulated), XIST (downregulated), and RPS4Y1 (upregulated) were commonly expressed in all four cell clusters. The DEGs in microglial cells and astrocytes were enriched in the IL‐17 signaling pathway. Conclusion: Our study explored differences in cells found in a patient with PE compared to a patient with HE, and the transcriptome in the different cells was analyzed for the first time. Studying inflammatory and immune functions might be the best approach for investigating traumatic temporal lobe epilepsy in neurons. [ABSTRACT FROM AUTHOR]

Ellagic acid is one of the most representative natural antioxidants, and is rich in pomegranate peel. In this study, a consecutive countercurrent chromatographic (CCC) separation method was established to improve the preparative efficiency of ellagic acid from pomegranate peel. By optimizing the solvent system, sample size and flow rate, 280 mg of ellagic acid was obtained from 5 g of crude sample from pomegranate peel by CCC after six consecutive injections. Moreover, the values of EC50 for ellagic acid in scavenging ABTS·+ and DPPH· were 4.59 ± 0.07 and 10.54 ± 0.07 μg/ml, respectively, indicating a strong antioxidant activity. This study not only established a high‐throughput method for the preparation of ellagic acid, but also provided a successful example for the development of and research on other natural antioxidants. [ABSTRACT FROM AUTHOR]

The Tianshui area is located at the junction of the Qinling and Qilian Orogens, the evolution of which is crucial in understanding the Early Palaeozoic tectonic evolution of the Central China Orogenic System. We present here geochronological, geochemical, and Sr-Nd isotopic data for four representative Silurian magmatic rocks, termed the Dangchuan, Huamiao, Yanjiadian, and Hongtupu, along a cross section across the main rock units of the Tianhui area. The 432 Ma Dangchuan syenogranites are peraluminous with negative εNd(t) values, high initial 87Sr/86Sr ratios, and low Rb/Sr and high CaO/Na2O ratios. We interpret the syenogranites to be generated by partial melting of metagreywackes during a Silurian orogenic event. The 436 Ma Huamiao diorites are high-Mg diorites with high MgO contents and Mg# (63–65) and enriched Sr-Nd isotopic compositions. The 445 Ma Yanjiadian diorites are high-Mg adakitic rocks with high Sr and Mg# and low Y and Yb contents. They were both generated by the interaction between sediment/slab-derived fluids/melts and mantle peridotite in the subduction-related setting. The 445 Ma Hongtupu basalts include E-MORB and IAB with depleted Sr-Nd isotopic compositions. They were derived from enriched mantle and sediment-modified mantle, respectively, and formed in a back-arc basin setting. Synthesizing our new and published data, we propose a tectonic model involving generation of Baihua-Huluhe arc-back-arc system, subduction of the Huluhe back-arc oceanic basin, and collision between the North Qinling arc terrane and North China Block, which is closely associated with the closure of the Proto-Tethys Ocean. [ABSTRACT FROM AUTHOR]

AbstractCoprinus comatus is an edible mushroom and its fermented product possesses antioxidant activity. In this study, to further enhance the antioxidant activity and improve the reusability of the strain, calcium alginate hydrogel was used as the carrier for embedding and immobilizing Coprinus comatus. The effects of CaCl2 concentration, sodium alginate concentration, microsphere diameter, and the amount of magnetic particle on the antioxidant activity of fermented products were investigated. The results showed that the magnetic immobilized microsphere prepared by 2.50% CaCl2, 2.00% sodium alginate and 0.50% Fe3O4 had the best fermentation antioxidant activity (EC50 was 0.43 ± 0.01 mg/mL) when the diameter was 5 mm, which increased by 24.56% compared to the initial activity. Besides, the microsphere showed strong reusability, the antioxidant activity was still better than the free strain after being used five times. This study not only enhanced the antioxidant activity of Coprinus comatus fermented product through immobilization, but also provided an effective method for microbial fermentation. [ABSTRACT FROM AUTHOR]

Not available.