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Unemployment insurances are perceived as major benefits to support workers in their job findings. This thesis contributes to the pre-existent literature through researching the impact of the unemployment benefits on the companies’ hirings and permanent layoffs, due to a lack of research on this matter. Using state and firm-specific variables, this study analyses the absolute and percentage impact on number of companies’ employees. For two of the overall performed regression models, higher amounts of unemployment insurances seem to impact positively the number of companies’ employees.

A presente dissertação tem como objetivo principal o estudo do impacto do marketing de conteúdo no e-commerce. Este tema foi escolhido tendo em conta a sua crescente importância do tema na sociedade e a falta de estudos específicos nesta área em contexto português. O estudo foca-se nos elementos do entretenimento, da confiança, do seu valor e do propósito do marketing de conteúdo e do seu impacto no uso do e-commerce. A investigação foi conduzida em formato de análise quantitativa através de questionário e os resultados foram analisados através do software SPSS (Statistical Package for the Social Sciences). Os resultados desta investigação demonstram que a confiança é o elemento do marketing de conteúdo que mais influencia o uso do ecommerce, com repercussões na forma como as empresas e as agências devem apostar para induzir a compra online. The main objective of this dissertation is to study the impact of content marketing on e-commerce. This theme was chosen taking into account its growing importance in society and the lack of specific studies in this area in the Portuguese context. The study focuses on the elements of entertainment, trust, its value and purpose in content marketing and its impact on the use of e-commerce. The investigation was conducted in a quantitative analysis format using a questionnaire and the results were analyzed using the SPSS software (Statistical Package for the Social Sciences). The results of this investigation demonstrate that trust is the content marketing element that most influences the use of e-commerce, with repercussions on the way companies and agencies should bet to induce online purchases.

Increased expression of transketolase (TKT) and its isoform transketolase-like-1 (TKTL1) has been related to the malignant leukemia phenotype through promoting an increase in the non-oxidative branch of the pentose phosphate pathway (PPP). Recently, it has also been described that TKTL1 can have a role in survival under hypoxic conditions and in the acquisition of radio resistance. However, TKTL1’s role in triggering metabolic reprogramming under hypoxia in leukemia cells has never been characterized. Using THP-1 AML cells, and by combining metabolomics and transcriptomics techniques, we characterized the impact of TKTL1 knockdown on the metabolic reprogramming triggered by hypoxia. Results demonstrated that TKTL1 knockdown results in a decrease in TKT, glucose-6-phosphate dehydrogenase (G6PD) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activities and impairs the hypoxia-induced overexpression of G6PD and GAPDH, all having significant impacts on the redox capacity of NADPH- and NADH-related cells. Moreover, TKTL1 knockdown impedes hypoxia-induced transcription of genes encoding key enzymes and transporters involved in glucose, PPP and amino acid metabolism, rendering cells unable to switch to enhanced glycolysis under hypoxia. Altogether, our results show that TKTL1 plays a key role in the metabolic adaptation to hypoxia in THP-1 AML cells through modulation of G6PD and GAPDH activities, both regulating glucose/glutamine consumption and the transcriptomic overexpression of key players of PPP, glucose and amino acids metabolism.