8 results on '"Jacob-Dubuisson, Françoise"'
Search Results
2. Posttranscriptional Regulation by Copper with a New Upstream Open Reading Frame
- Author
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Roy, Gauthier, primary, Antoine, Rudy, additional, Schwartz, Annie, additional, Slupek, Stéphanie, additional, Rivera-Millot, Alex, additional, Boudvillain, Marc, additional, and Jacob-Dubuisson, Françoise, additional
- Published
- 2022
- Full Text
- View/download PDF
3. Large-Scale Conformational Changes of FhaC Provide Insights Into the Two-Partner Secretion Mechanism
- Author
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Sicoli, Giuseppe, primary, Konijnenberg, Albert, additional, Guérin, Jérémy, additional, Hessmann, Steve, additional, Del Nero, Elise, additional, Hernandez-Alba, Oscar, additional, Lecher, Sophie, additional, Rouaut, Guillaume, additional, Müggenburg, Linn, additional, Vezin, Hervé, additional, Cianférani, Sarah, additional, Sobott, Frank, additional, Schneider, Robert, additional, and Jacob-Dubuisson, Françoise, additional
- Published
- 2022
- Full Text
- View/download PDF
4. Pathogenicity and virulence of Bordetella pertussis and its adaptation to its strictly human host
- Author
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Belcher, Thomas, primary, Dubois, Violaine, additional, Rivera-Millot, Alex, additional, Locht, Camille, additional, and Jacob-Dubuisson, Françoise, additional
- Published
- 2021
- Full Text
- View/download PDF
5. Pathogenicity and virulence of Bordetella pertussisand its adaptation to its strictly human host
- Author
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Belcher, Thomas, Dubois, Violaine, Rivera-Millot, Alex, Locht, Camille, and Jacob-Dubuisson, Françoise
- Abstract
ABSTRACTThe highly contagious whooping cough agent Bordetella pertussishas evolved as a human-restricted pathogen from a progenitor which also gave rise to Bordetella parapertussisand Bordetella bronchiseptica. While the latter colonizes a broad range of mammals and is able to survive in the environment, B. pertussishas lost its ability to survive outside its host through massive genome decay. Instead, it has become a highly successful human pathogen by the acquisition of tightly regulated virulence factors and evolutionary adaptation of its metabolism to its particular niche. By the deployment of an arsenal of highly sophisticated virulence factors it overcomes many of the innate immune defenses. It also interferes with vaccine-induced adaptive immunity by various mechanisms. Here, we review data from invitro, human and animal models to illustrate the mechanisms of adaptation to the human respiratory tract and provide evidence of ongoing evolutionary adaptation as a highly successful human pathogen.
- Published
- 2021
- Full Text
- View/download PDF
6. Posttranscriptional Regulation by Copper with a New Upstream Open Reading Frame
- Author
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Gauthier Roy, Rudy Antoine, Annie Schwartz, Stéphanie Slupek, Alex Rivera-Millot, Marc Boudvillain, Françoise Jacob-Dubuisson, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Jacob-Dubuisson, Françoise
- Subjects
upstream ORF ,copper homeostasis ,posttranscriptional regulation ,Gene Expression Regulation, Bacterial ,Microbiology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Bordetella pertussis ,Open Reading Frames ,Bacterial Proteins ,Gene Expression Regulation ,Virology ,DUF2946 family ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Operon ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Ribosomes ,Copper - Abstract
International audience; Copper is essential to most living beings but also highly toxic and as such is an important player at the host-pathogen interface. Bacteria have thus developed homeostatic mechanisms to tightly control its intracellular concentration. Known Cu export and import systems are under transcriptional control, whereas post-transcriptional regulatory mechanisms are yet to be characterized. We identified a three-gene operon, bp2923-bfrG-bp2921, down-regulated by copper and notably encoding a TonB-dependent transporter in Bordetella pertussis. We show here that the protein encoded by the first gene, which is a member of the DUF2946 protein family, represents a new type of upstream Open Reading Frame (uORF) involved in post-transcriptional regulation of the downstream genes. In the absence of copper, the entire operon is transcribed and translated. Perception of copper by the nascent bp2923-coded protein via its conserved CXXC motif triggers Rho-dependent transcription termination between the first and second genes by relieving translation arrest on a conserved C-terminal RAPP motif. Homologs of bp2923 are widespread in bacterial genomes, where they head operons predicted to participate in copper homeostasis. This work has thus unveiled a new mode of genetic regulation by a transition metal and identified a regulatory function for a member of an uncharacterized family of bacterial proteins that we have named CruR, for copper-responsive upstream regulator.
- Published
- 2022
7. Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanism
- Author
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S. Lecher, Robert Schneider, G. Rouaut, Hervé Vezin, Françoise Jacob-Dubuisson, L. Müggenburg, Oscar Hernandez-Alba, Frank Sobott, E. Del Nero, Sarah Cianférani, J. Guerin, G. Sicoli, Steve Hessmann, Albert Konijnenberg, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE], University of Antwerp [UA], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL], Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] [LSMBO], Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE], University of Leeds, Université de Lille, CNRS, Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516, Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017, Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), University of Antwerp (UA), Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Infrastructure Nationale de Protéomique, FR2048 ProFI, Biologie Structurale Intégrative (ERL 9002 - BSI ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), CHU Lille, Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), and Jacob-Dubuisson, Françoise
- Subjects
[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Beta hairpin ,Omp85 superfamily ,Gramnegative bacteria ,010402 general chemistry ,two-partner secretion system ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,01 natural sciences ,outer membrane protein ,03 medical and health sciences ,[CHIM]Chemical Sciences ,Secretion ,Lipid bilayer ,Molecular Biology ,Biology ,030304 developmental biology ,mass spectrometry ,0303 health sciences ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Chemistry ,Periplasmic space ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Transmembrane protein ,NMR ,0104 chemical sciences ,Transport protein ,[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry ,[CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistry ,Beta barrel ,protein dynamics ,Biophysics ,EPR ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Bacterial outer membrane - Abstract
The Two-Partner secretion pathway mediates protein transport across the outer membrane of Gram-negative bacteria. TpsB transporters belong to the Omp85 superfamily, whose members catalyze protein insertion into, or translocation across membranes without external energy sources. They are composed of a transmembrane β barrel preceded by two periplasmic POTRA domains that bind the incoming protein substrate. Here we used an integrative approach combining in vivo assays, mass spectrometry, nuclear magnetic resonance and electron paramagnetic resonance techniques suitable to detect minor states in heterogeneous populations, to explore transient conformers of the TpsB transporter FhaC. This revealed substantial, spontaneous conformational changes with a portion of the POTRA2 domain coming close to the lipid bilayer and surface loops. Specifically, the amphipathic β hairpin immediately preceding the first barrel strand can insert into the β barrel. We propose that these motions enlarge the channel and hoist the substrate into it for secretion. An anchor region at the interface of the β barrel and the POTRA2 domain stabilizes the transporter in the course of secretion. Our data propose a solution to the conundrum how these transporters mediate protein secretion without the need for cofactors, by utilizing intrinsic protein dynamics.
- Published
- 2021
8. Pathogenicity and virulence of Bordetella pertussis and its adaptation to its strictly human host
- Author
-
Alex Rivera-Millot, Camille Locht, Françoise Jacob-Dubuisson, Thomas Belcher, Violaine Dubois, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), and Jacob-Dubuisson, Françoise
- Subjects
Microbiology (medical) ,Bordetella pertussis ,Bordetella ,Immunology ,virulence factors ,Virulence ,Human pathogen ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,Bordetella parapertussis ,03 medical and health sciences ,Pertussis ,evolution ,Pathogen ,innate immunity ,030304 developmental biology ,Genetics ,0303 health sciences ,Bordetella bronchiseptica ,biology ,030306 microbiology ,adaptive immunity ,biology.organism_classification ,Acquired immune system ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Infectious Diseases ,Parasitology ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,metabolism - Abstract
International audience; The highly contagious whooping cough agent Bordetella pertussis has evolved as a humanrestricted pathogen from a progenitor which also gave rise to Bordetella parapertussis and Bordetella bronchiseptica. While the latter colonizes a broad range of mammals and is able to survive in the environment, B. pertussis has lost its ability to survive outside its host through massive genome decay. Instead, it has become a highly successful human pathogen by the acquisition of tightly regulated virulence factors and evolutionary adaptation of its metabolism to its particular niche. By the deployment of an arsenal of highly sophisticated virulence factors it overcomes many of the innate immune defenses. It also interferes with vaccine-induced adaptive immunity by various mechanisms. Here, we review data from in vitro, human and animal models to illustrate the mechanisms of adaptation to the human respiratory tract and provide evidence of ongoing evolutionary adaptation as a highly successful human pathogen.
- Published
- 2021
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