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Microeukaryotes are key contributors to marine carbon cycling. Their physiology, ecology, and interactions with the chemical environment are poorly understood in offshore ecosystems, and especially in the deep ocean. Using the Autonomous Underwater Vehicle Clio, microbial communities along a 1050 km transect in the western North Atlantic Ocean were surveyed at 10–200 m vertical depth increments to capture metabolic signatures spanning oligotrophic, continental margin, and productive coastal ecosystems. Microeukaryotes were examined using a paired metatranscriptomic and metaproteomic approach. Here we show a diverse surface assemblage consisting of stramenopiles, dinoflagellates and ciliates represented in both the transcript and protein fractions, with foraminifera, radiolaria, picozoa, and discoba proteins enriched at >200 m, and fungal proteins emerging in waters >3000 m. In the broad microeukaryote community, nitrogen stress biomarkers were found at coastal sites, with phosphorus stress biomarkers offshore. This multi-omics dataset broadens our understanding of how microeukaryotic taxa and their functional processes are structured along environmental gradients of temperature, light, and nutrients.Using an autonomous underwater vehicle, this study presents an integrated biogeochemical and multiomic analysis of microbial eukaryotes from the North Atlantic Ocean. The work highlights diverse communities that shift through depth zones, with signatures of nutrient biomarkers changing across a coastal-offshore spatial gradient. [ABSTRACT FROM AUTHOR]

Optical fiber is increasingly used for both communication and distributed sensing of temperature and strain in environmental studies. In this work, we demonstrate the viability of unreinforced fiber tethers (bare fiber) for Raman-based distributed temperature sensing in deep ocean and deep ice environments. High-pressure testing of single-mode and multimode optical fiber showed little to no changes in light attenuation over pressures from atmospheric to 600 bars. Most importantly, the differential attenuation between Stokes and anti-Stokes frequencies, critical for the evaluation of distributed temperature sensing, was shown to be insignificantly affected by fluid pressures over the range of pressures tested for single-mode fiber, and only very slightly affected in multimode fiber. For multimode fiber deployments to ocean depths as great as 6000 m, the effect of pressure-dependent differential attenuation was shown to impact the estimated temperatures by only 0.15 °K. These new results indicate that bare fiber tethers, in addition to use for communication, can be used for distributed temperature or strain in fibers subjected to large depth (pressure) in varying environments such as deep oceans, glaciers and potentially the icy moons of Saturn and Jupiter. [ABSTRACT FROM AUTHOR]