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Ethnopharmacological Relevance: Ischemic stroke causes damages to both gray and white matter, resulting in long-term motor impairments. Myelin repair is a promising strategy for poststroke motor rehabilitation. Buyang Huanwu Decoction (BHD) is a classical traditional Chinese medicine formula for managing the sequelae of ischemic stroke. Whether BHD benefits gray and white matter remyelination following stroke remains to be elucidated., Aim of the Study: The present study aimed to investigate the effects of BHD on the gray and white matter remyelination following ischemic stroke and further explore the underlying mechanisms by combining magnetic resonance imaging (MRI) and histological experiments., Materials and Methods: The ischemic stroke model was established in male Sprague-Dawley rats by permanently occluding the middle cerebral artery (MCAO). BHD (16.6 g/kg and 8.3 g/kg) was intragastrically administered to rats for 30 days. The motor function was assessed by an automated Digi gait system. The structural integrity of the motor cortex and external capsule was monitored by MRI, including T2 mapping and diffusion tensor imaging (DTI). The remyelination was examined by Olig2/Ki67, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)/Ki67 double immunofluorescence staining and Luxol fast blue (LFB) staining. Subsequently, the Notch signaling activation in astrocytes and microglia was assessed by double immunofluorescence staining with JAG1/Notch1/Notch intracellular domain (NICD) and glial fibrillary acidic protein (GFAP)/ionized calcium binding adaptor molecule 1 (Iba1)., Results: BHD treatments remarkably improved motor function of the MCAO rats by reducing steps, swing time and ataxia coefficient of the left forelimb. The MRI examinations found that BHD treatments significantly reduced infarct volume and preserved the motor cortex and external capsule integrity, as reflected by decreased T2 values, RD, and increased FA. Notably, the gait parameters of the left forelimb were correlated to the MRI index obtained from the perilesional motor cortex and external capsule to varying degrees. Furthermore, BHD treatments enhanced gray and matter remyelination by elevating the numbers of Olig2 + /Ki67 + , CNPase + /Ki67 + cells, and the integrated optical density of LFB. Finally, BHD effectively inhibited the activation of Notch signaling in the astrocytes and microglia of the corresponding gray and white matter, as evidenced by decreased numbers of cells co-expressing JAG1/Notch1/NICD and GFAP/Iba1., Conclusion: This study demonstrated that BHD treatment could promote poststroke motor recovery by preserving the structural integrity of the gray and white matter and facilitating their remyelination. Notably, the pro-remyelination effects of BHD treatment might be attributed to suppressed activation of Notch signaling within the reactive astrocytes and microglia., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2025 Elsevier B.V. All rights reserved.)

Ethnopharmacological Relevance: Astragaloside IV (AS), a key active ingredient obtained from Chinese herb Astragalus mongholicus Bunge, exerts potent neuroprotective and anti-inflammatory effects for treating neurodegenerative diseases. However, mechanisms of AS on improvement of ischemic brain tissue repair remain unclear., Aim of the Study: This research aims at using magnetic resonance imaging (MRI) to noninvasively determine whether AS facilitates brain tissue repair, and investigating whether AS exerts brain remodeling through adenosine monophosphate-activated protein kinase (AMPK) metabolic signaling regulating key glycolytic enzymes and energy transporters, thereby impacting microglia polarization., Materials and Methods: Ischemic stroke model in male Sprague-Dawley rats were induced through permanent occlusion of the middle cerebral artery (MCAO). Infarct volume, the alterations of brain microstructure and nerve fibers reorganization were examined by multi-parametric MRI. The pathological damages of myelinated axons and microglia polarization surrounding infarct tissue were detected using pathological techniques. Furthermore, M1/M2 microglia polarization associated protein, glycolytic rate-limiting enzymes, energy transporters and AMPK/mammalian target of rapamycin (mTOR)/hypoxia inducible factor-1α (HIF-1α) signal were examined both in ischemic stroke rats and BV2 microglia treated with lipopolysaccharide (LPS) + interferon-γ (IFN-γ) by western blotting., Results: MRI revealed that AS obviously decreased infarct volume, relieved brain microstructure damage and improved nerve fibers reorganization in ischemic stroke rats. Histological tests supported MRI findings. Notably, AS promoted microglia M2 and reduced M1 polarization, induced the AMPK activation accompanied with decreased levels of phosphorylated mTOR and HIF-1α. Moreover, AS suppressed the expression of glycolytic rate-limiting enzymes and energy transporters in ischemic stroke rats and BV2 microglia. In contrast, these beneficial effects were greatly blocked by AMPK inhibitor compound C., Conclusion: Overall, these results collectively suggested that AS facilitated tissue remodeling that may be partially through modulating polarization of microglia in AMPK- dependent metabolic pathways after ischemic stroke., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Ethnopharmacological Relevance: Buyang Huanwu Decoction (BYHWD), one of the most commonly utilized traditional Chinese medicine prescription for treatment of cerebral ischemic stroke. However, the understanding of BYHWD on neurovascular repair following cerebral ischemia is so far limited., Aim of the Study: This research investigated the influence of BYHWD on neurovascular remodeling by magnetic resonance imaging (MRI) technology and revealed the potential neurovascular repair mechanism underlying post-treatment with BYHWD after ischemic stroke., Materials and Methods: Male Sprague-Dawley rats were utilized as an ischemic stroke model by permanent occlusion of the middle cerebral artery (MCAO). BYHWD was intragastrically administrated once daily for 30 days straight. Multimodal MRI was performed to detect brain tissue injuries, axonal microstructural damages, cerebral blood flow and intracranial vessels on the 30th day after BYHWD treatment. Proangiogenic factors, axonal/synaptic plasticity-related factors, energy transporters and adenosine monophosphate-activated protein kinase (AMPK) signal pathway were evaluated using western blot. Double immunofluorescent staining and western blot were applied to evaluate astrocytes and microglia polarization., Results: Administration of BYHWD significantly alleviated infarct volume and brain tissue injuries and ameliorated microstructural damages, accompanied with improved axonal/synaptic plasticity-related factors, axonal growth guidance factors and decreased axonal growth inhibitors. Meanwhile, BYHWD remarkably improved cerebral blood flow, cerebral vascular signal and promoted the expression of proangiogenic factors. Particularly, treatment with BYHWD obviously suppressed astrocytes A1 and microglia M1 polarization accompanied with promoted astrocyte A2 and microglia M2 polarization. Furthermore, BYHWD effectively improved energy transporters. Especially, BYHWD markedly increased expression of phosphorylated AMPK, cyclic AMP-response element binding protein (CREB) and brain-derived neurotrophic factor (BDNF) accompanied by inactivation of the NF-κB., Conclusion: Taken together, these findings identified that the beneficial roles of BYHWD on neurovascular remodeling were related to AMPK pathways -mediated energy transporters and NFκB/CREB pathways., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Production of flexible strain sensors is complex, time-consuming, and expensive. In this study, a novel fabrication method of polydimethylsiloxane/graphene nanocomposite conductive materials was proposed by using the scraping and coating method for manufacturing sandwich-shape flexible strain sensors. A ZQ-60B tensile testing machine was employed to test the mechanical properties of flexible sensors with 1%, 3%, and 5% graphene content. The results revealed that the stress and strain of the flexible strain sensor exhibited a linear relationship, and the linear correlation coefficients were 0.99706, 0.99819, and 0.99826, respectively. The concentration of graphene was 1%, 3%, and 5%, and the gauge factors (GFs) of the sensor were 24, 6, and 3, respectively. With the increase in the graphene content, the GF decreased gradually. This phenomenon could be attributed to tunneling, which increased the number of conductive pathways with an increase in the graphene content. Furthermore, the sensor exhibited excellent stability after 100 cycles of stretching/scaling. The finger joint bending test revealed that the flexible strain sensor is reproducible and exhibits excellent application prospects in monitoring human movement and health.

The aim of this study was to investigate the abnormities in functional connectivity (FC) within each modular network and between modular networks in patients with systemic lupus erythematosus (SLE). Twelve meaningful modular networks were identified via independent component analysis from 41 patients and 40 volunteers. Parametric tests were used to compare the intra- and intermodular FC between the groups. Partial correlation analysis was used to seek the relationships between abnormal FCs and the clinical data. Compared to the controls, SLE patients showed decreased intramodular FC in the anterior default mode network (aDMN), posterior default mode network (pDMN), ventral attention network (VAN), and sensorimotor network (SMN) and increased intramodular FC in the medial visual network (mVN) and left frontoparietal network. In addition, SLE patients showed decreased intermodular FC between the SMN and the lateral visual network (lVN), between the SMN and the VAN, and between the pDMN and the lVN and exhibited increased intermodular FC between the SMN and the salience network (SAN), between the pDMN and the SAN, and between the aDMN and the VAN. Moreover, we found several correlations among the abnormal FCs and the Mini-Mental State Examination in SLE patients. Mild cognitive impairment is compensated by the hyperconnectivity between the aDMN and the VAN, while severe cognitive impairment tends to be compensated by the hyperconnectivity between the SMN and the SAN. The FC value between the SMN and the SAN and between the aDMN and the VAN may serve as neuroimaging markers for monitoring cognitive progression in SLE patients., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)