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Systemic lupus erythematosus (SLE) is a complex autoimmune condition that disproportionately impacts non-White ethnic and racial groups, particularly individuals in the African diaspora who experience heightened incidence, prevalence, and adverse outcomes. Genetic and epigenetic factors play significant roles in SLE risk, however these factors neither explain the whole of SLE risk nor the stark racial disparities we observe. Moreover, our understanding of genetic risk factors within African ancestry populations is limited due to social and environmental influences on research participation, disease presentation, and healthcare access. Globally, the African diaspora faces barriers in accessing essential SLE diagnostic tools, therapeutics, healthcare practitioners, and high-quality clinical and translational research studies. Here, we provide insights into the current state of genetic studies within African ancestry populations and highlight the unique challenges encountered in SLE care and research across countries of varying income levels. We also identify opportunities to address these disparities and promote scientific equity for individuals affected by SLE within the global African diaspora.

The BipA protein is a universally conserved GTPase in bacterial species and is structurally similar to translational GTPases. Despite its wide distribution, BipA is dispensable for growth under optimal growth conditions but is required under stress conditions. In particular, bipA-deleted cells (ESC19) have been shown to display a variety of phenotypic changes in ribosome assembly, capsule production, lipopolysaccharide (LPS) synthesis, biofilm formation, and motility at low temperature, suggesting its global regulatory roles in cold adaptation. Here, through genomic library screening, we found a suppressor clone containing nhaR, which encodes a Na+-responsive LysR-type transcriptional regulator and whose gene product partially restored the growth of strain ESC19 at 20°C. The suppressed cells showed slightly reduced capsule production and improved biofilm-forming ability at 20°C, whereas the defects in the LPS core and swimming motility were not restored but aggravated by overexpression of nhaR. Notably, the overexpression partially alleviated the defects in 50S ribosomal subunit assembly and rRNA processing of ESC19 cells by enhancing the overall transcription of rRNA. Electrophoretic mobility shift assay revealed the association of NhaR with the promoter of seven rrn operons, suggesting that NhaR directly regulates rRNA transcription in ESC19 at 20°C. The suppressive effects of NhaR on ribosomes, capsules, and LPS were dependent on its DNA-binding activity, implying that NhaR might be a transcriptional factor involved in regulating these genes at 20°C. Furthermore, we found that BipA may be involved in adaptation to salt stress, designating BipA as a global stress-responsive regulator, as the deletion of bipA led to growth defects at 37°C and high Na+ concentrations without ribosomal defects.

The aim of this study was to investigate whether individualized low-intensity exercise (ILIE) within the recovery domain before lactate threshold 1 (LT 1) improves energetic recovery and general endurance capacity in professional soccer players. Twenty-four professional soccer players (age: 24.53 ± 4.85 years, height: 180 ± 6.30 cm, body mass: 75.86 ± 8.01 kg, body fat: 12.19 ± 2.69%) participated in the study (n = 24). The 1-h ILIE intervention involved 27 jogging sessions spanning nine weeks and jogging speed corresponding to 72% of LT 1 (7.15 ± 0.95 km∙h-1). Pre-ILIE and post-ILIE LT testing variables measured within 9 weeks included blood lactate concentrations (La-) and heart rate (HR) at specific exercise intensities during ILIE LT test. The jogging/running speeds (S), delta (Δ) S, HR, and ΔHR were measured at 1.5, 2.0, 3.0, and 4.0 mmol∙L-1 La-, respectively. Values of La- and HR at the same exercise intensities (5.4-16.2 km∙h-1) in the post-ILIE LT test compared with pre-ILIE LT test were significantly decreased (P < 0.05 and P < 0.01, respectively). Furthermore, S at all specific La- levels (1.5, 2.0, 3.0, and 4.0) were significantly increased, while HR at 2.0, 3.0, and 4.0 La- decreased significantly (P < 0.05 and P < 0.01, respectively). Low to moderate positive correlations were observed between ΔS and ΔHR at 1.5 and 2.0 La- (r = 0.52 and r = 0.40, respectively). The nine-week ILIE improved energy recovery and general endurance of professional soccer players. This relates to repeated high-intensity intermittent sprints during the 90-min soccer game.

Lithium-ion batteries are commonly used in electric vehicles, mobile phones, and laptops because of their environmentally friendly nature, high energy density, and long lifespan. Despite these advantages, lithium-ion batteries may experience overcharging or discharging if they are not continuously monitored, leading to fire and explosion risks, in cases of overcharging, and decreased capacity and lifespan, in cases of overdischarging. Another factor that can decrease the capacity of these batteries is their internal resistance, which varies with temperature. This study proposes an estimation method for the state of charge (SOC) using a neural network (NN) model that is highly applicable to the external temperatures of batteries. Data from a vehicle-driving simulator were used to collect battery data at temperatures of 25 °C, 30 °C, 35 °C, and 40 °C, including voltage, current, temperature, and time data. These data were used as inputs to generate the NN models. The NNs used to generate the model included the multilayer neural network (MNN), long short-term memory (LSTM), gated recurrent unit (GRU), and gradient boosting machine (GBM). The SOC of the battery was estimated using the model generated with a suitable temperature parameter and another model generated using all the data, regardless of the temperature parameter. The performance of the proposed method was confirmed, and the SOC-estimation results demonstrated that the average absolute errors of the proposed method were superior to those of the conventional technique. In the estimation of the battery’s state of charge in real time using a Jetson Nano device, an average error of 2.26% was obtained when using the GRU-based model. This method can optimize battery performance, extend battery life, and maintain a high level of safety. It is expected to have a considerable impact on multiple environments and industries, such as electric vehicles, mobile phones, and laptops, by taking advantage of the lightweight and miniaturized form of the Jetson Nano device.

Does Kim Jong Un need nuclear weapons to deter the United States? Pyongyang has long insisted that nuclear weapons prevent the United States from invading North Korea. But, do nuclear weapons provide Pyongyang with as much deterrent effect as it expects? Has the conventional military posture failed in deterring the U.S.-ROK alliance? This article challenges conventional wisdom on North Korean nuclear deterrence and raises the question of why nuclear weapons do not help Pyongyang improve its deterrence capability. Pyongyang's pursuit of nuclear deterrence is an overdeterrence. Pyongyang's presumably assured retaliation is not credible given the United States' superb nuclear capability. Rather, conventional deterrence has worked on the Korean peninsula. Pyongyang may have other reasons to build up its nuclear capability, but the risks and costs are too high to take. This article also suggests highly important and timely theoretical and policy implications about how to deal with a nuclear North Korea. [ABSTRACT FROM AUTHOR]

Mycobacterium smegmatis has two isocitrate lyase (ICL) isozymes (MSMEG_0911 and MSMEG_3706). We demonstrated that ICL1 (MSMEG_0911) is the predominantly expressed ICL in M. smegmatis and plays a major role in growth on acetate or fatty acid as the sole carbon and energy source. Expression of the icl1 gene in M. smegmatis was demonstrated to be strongly upregulated during growth on acetate relative to that in M. smegmatis grown on glucose. Expression of icl1 was shown to be positively regulated by the RamB activator, and three RamB-binding sites (RamBS1, RamBS2, and RamBS3) were identified in the upstream region of icl1 using DNase I footprinting analysis. Succinyl coenzyme A (succinyl-CoA) was shown to increase the affinity of binding of RamB to its binding sites and enable RamB to bind to RamBS2, which is the most important site for RamB-mediated induction of icl1 expression. These results suggest that succinyl-CoA serves as a coinducer molecule for RamB. Our study also showed that cAMP receptor protein (Crp1; MSMEG_6189) represses icl1 expression in M. smegmatis grown in the presence of glucose. Therefore, the strong induction of icl1 expression during growth on acetate as the sole carbon source relative to the weak expression of icl1 during growth on glucose is likely to result from combined effects of RamB-mediated induction of icl1 in the presence of acetate and Crp-mediated repression of icl1 in the presence of glucose. [ABSTRACT FROM AUTHOR]