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Very little published information is available on the scientific identification of wood species used in the construction of boats in ancient Egypt. This paper deals with the scientific identification of wood species used in the construction of the deckhouse, canopy, and forecastle of King Khufu’s second wooden boat (4th Dynasty—Old Kingdom) in detail. This paper also discusses the principal technological characteristics of the identified woods, considering specifically their utilization for construction of the deckhouse, canopy, and forecastle. Almost all the woods used in the boat’s deckhouse, canopy, and forecastle were made of two imported species of wood (Cedrus libani A.Rich. and Juniperus sp.), with two native species (Ziziphus spina-christi (L.) Willd. and Vachellia sp.) also identified. The analysis most surprisingly revealed the presence of Ziziphus spina-christi (L.) Willd. in 25% of the analyzed forecastle samples, which was discovered for the first time for making cross beams in the construction of boats in ancient Egypt. Another intriguing aspect of the boat’s construction is the presence of Juniperus sp., which surprisingly showed that almost 85% of the analyzed samples were Juniperus sp., used in the deckhouse’s boards, frames, and cross beams. The data let us examine the specific employment of the wood species used in the parts of the boat, which evidenced that the identified woods were suitably used for the construction of the different parts of the deckhouse, canopy, and forecastle of the boat.