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MicroRNA-139-5p (miR-139-5p) plays a crucial role in cancer’s onset and progression and is considered a tumor suppressor gene. However, the biological functions and mechanism of miR-139-5p in the tumorigenicity in Non-small cell lung cancer (NSCLC) has received insufficient investigation. We investigated the miR-139-5p expression in lung cancer tissues and the adjacent normal lung tissues, in 54 NSCLC patients. Additionally, the miR-139-5p target gene was predicted by bioinformatics analysis and then confirmed by a dual-luciferase reporter assay. The biological functions of miR-139-5p and the target gene have been investigated, in both in vitro and in vivo studies. Finally, the anti-tumor effects of miR-139-5p were corroborated by tumor formation assay in nude mice and progression-free survival analysis in another 144 NSCLC patients. MiR-139-5p was down-regulated, and inversely correlated with DNA topoisomerase II alpha (TOP2A), in both NSCLC tissues and cells. In vitro, miR-139-5p overexpression suppressed proliferation, migration, and invasion by down-regulating TOP2A in normal human bronchial epithelioid cells and NSCLC cell lines. It also induced apoptosis in NSCLC cell lines. In vivo, miR-139-5p up-regulation and the TOP2A mRNA down-regulation were significantly correlated, with longer progression-free survival times in 144 NSCLC patients. Moreover, miR-139-5p overexpression mitigated the xenograft tumor formation. MiR-139-5p inhibited growth and metastasis in NSCLC cell lines via direct TOP2A targeting. Low miR-139-5p expression showed a significant correlation with poor prognosis in NSCLC patients. These results highlight the effect of miR-139-5p on NSCLC, suggesting that miR-139-5p is a promising biomarker for NSCLC prognosis and an exceptional candidate for further therapeutic exploration.

Firefighters exhibit a higher prevalence of mental health issues than other occupations because they often directly or indirectly experience potentially traumatic events. Previous research has suggested that recovery experiences, sleep, and fatigue may be protective or risk factors for firefighters' mental health. This study conducted regularised partial correlation network analysis to investigate and visualise the complex relationships between these variables. We collected data through an online survey, and 3144 career firefighters from a large east-coast city in China, were included in the analyses. The results of the network analysis showed that: (1) only relaxation and mastery experiences directly correlated with one dimension of the mental health scale, namely, life/social functioning; (2) sleep quality had stronger correlations with mental health issues than sleep quantity; and (3) mental health issues were central nodes of the network. These results indicated that firefighters' mental health was close to other variables in the network and acted as a bridge linking other variables. Therefore, more attention should be paid to the mental health of firefighters to prevent comorbidities. The study's results also indicated that recovery experiences and sleep may be protective factors for firefighters' mental health.

Annealing and cyclic voltammetry (CV) are essential for the activation of amorphous alloy ribbons. Various amorphous alloy ribbons have been activated in the fields of environmental catalysts using either annealing or CV. However, the combination of the two methods for improving the oxygen evolution reaction (OER) performance has rarely been reported. This combination is expected to significantly improve the OER performance of amorphous ribbons. Here, we developed an "annealing +CV-activation" integrated strategy to treat a free-standing NiFeBSiP ribbon, which as an efficient and stable oxygen-evolving electrode. The "annealing +CV-activation" strategy induces the nanocrystallization and oxidation effects on the surface of the NiFeBSiP ribbon. The effects significantly increase the electron transfer ability, the Ni/Fe/P oxidation state and the surface area of the NiFeBSiP ribbon, which consequently leads to enhancing the OER performance. As a result, the treated ribbon exhibits a low overpotential of 269 mV at 10 mA cm

Uric acid (UA) may play a crucial role in the process of cerebral small vessel disease (SVD), but few follow-up studies have focused on the effect of UA in the progression of SVD. The present study aimed to ascertain whether serum UA levels are associated with the risk of SVD progression.We performed an observational clinical study in adults older than 45 years with cranial magnetic resonance imaging (MRI) from 30 October 2015, to 28 January 2021. The patients were divided into two groups according to whether their total burden of SVD scores increased or not during the follow-up: SVD progression (increased by at least one point) and without SVD progression (increased 0 points). Cox regression and Kaplan-Meier survival analyses were used for univariate analysis between groups to identify the risk factors for SVD progression.Ultimately, 261 eligible patients were included in the final analysis. Of the 261 eligible patients, 73 were included in the SVD progression group, and 188 were included in the group without SVD progression. Correlation analysis found that the levels of UA and the ratio of hyperuricemia (HUA) showed statistically significant correlations with SVD progression risk (High serum UA levels are independently related to the risk of SVD progression, thus highlighting not only the influence of traditional risk factors such as hypertension and age on SVD but also the UA levels of patients for individualized treatment.