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Your search keyword '"Khosla, Aashima"' showing total 9 results
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9 results on '"Khosla, Aashima"'

1. The transcription factor ATML1 maintains giant cell identity by inducing synthesis of its own long-chain fatty acid-containing ligands

Author

Vadde, Batthula Vijaya Lakshmi, primary, Russell, Nicholas J., additional, Bagde, Saket Rahul, additional, Askey, Bryce, additional, Saint-Antoine, Michael, additional, Brownfield, Bryce, additional, Mughal, Salaiha, additional, Apprill, Lauren E., additional, Khosla, Aashima, additional, Clark, Frances K., additional, Schwarz, Erich M., additional, Alseekh, Saleh, additional, Fernie, Alisdair R., additional, Singh, Abhyudai, additional, Schrick, Kathrin, additional, Fromme, J. Christopher, additional, Skirycz, Aleksandra, additional, Formosa-Jordan, Pau, additional, and Roeder, Adrienne H. K., additional

Published
2024
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4. KARRIKIN UP-REGULATED F-BOX 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism in Arabidopsis thaliana

Author

Sepulveda, Claudia, primary, Guzmán, Michael A., additional, Li, Qingtian, additional, Villaécija-Aguilar, José Antonio, additional, Martinez, Stephanie E., additional, Kamran, Muhammad, additional, Khosla, Aashima, additional, Liu, Wei, additional, Gendron, Joshua M., additional, Gutjahr, Caroline, additional, Waters, Mark T., additional, and Nelson, David C., additional

Published
2022
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8. Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism

Author

Wojciechowska, Izabela, primary, Mukherjee, Thiya, additional, Knox-Brown, Patrick, additional, Hu, Xueyun, additional, Khosla, Aashima, additional, Mathews, Graham L., additional, Thompson, Kyle A., additional, Peery, Seth T., additional, Szlachetko, Jagoda, additional, Thalhammer, Anja, additional, Hincha, Dirk K., additional, Skirycz, Aleksandra, additional, and Schrick, Kathrin, additional

Published
2021
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9. Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism.

Author

Wojciechowska, Izabela, Mukherjee, Thiya, Knox‐Brown, Patrick, Hu, Xueyun, Khosla, Aashima, Subedi, Bibek, Ahmad, Bilal, Mathews, Graham L., Panagakis, Ashley A., Thompson, Kyle A., Peery, Sophie T., Szlachetko, Jagoda, Thalhammer, Anja, Hincha, Dirk K., Skirycz, Aleksandra, and Schrick, Kathrin

Subjects
*STEROIDOGENIC acute regulatory protein, *ARABIDOPSIS, *CELL culture, *TRANSCRIPTION factors, *MISSENSE mutation, *PHOSPHOLIPIDS
Abstract

Summary Plant homeodomain leucine zipper IV (HD‐Zip IV) transcription factors (TFs) contain an evolutionarily conserved steroidogenic acute regulatory protein (StAR)‐related lipid transfer (START) domain. While the START domain is required for TF activity, its presumed role as a lipid sensor is not clear. Here we used tandem affinity purification from Arabidopsis cell cultures to demonstrate that PROTODERMAL FACTOR2 (PDF2), a representative member that controls epidermal differentiation, recruits lysophosphatidylcholines (LysoPCs) in a START‐dependent manner. Microscale thermophoresis assays confirmed that a missense mutation in a predicted ligand contact site reduces lysophospholipid binding. We additionally found that PDF2 acts as a transcriptional regulator of phospholipid‐ and phosphate (Pi) starvation‐related genes and binds to a palindromic octamer with consensus to a Pi response element. Phospholipid homeostasis and elongation growth were altered in pdf2 mutants according to Pi availability. Cycloheximide chase experiments revealed a role for START in maintaining protein levels, and Pi starvation resulted in enhanced protein destabilization, suggesting a mechanism by which lipid binding controls TF activity. We propose that the START domain serves as a molecular sensor for membrane phospholipid status in the epidermis. Our data provide insights toward understanding how the lipid metabolome integrates Pi availability with gene expression. [ABSTRACT FROM AUTHOR]

Published
2024
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