1. Synergistic antifungal activity against Candida albicans between voriconazole and cyclosporine a loaded in polymeric nanoparticles.
- Author
-
Martín V, de la Haba RR, López-Cornejo P, López-López M, Antonio Lebrón J, Bernal E, Baeza N, Ruiz S, José Ostos F, Merino-Bohorquez V, Chevalier S, Lesouhaitier O, Tahrioui A, José Montes F, Sánchez-Carrasco T, and Luisa Moyá M more...
- Subjects
- Drug Carriers chemistry, Polyethylene Glycols chemistry, Microbial Sensitivity Tests, Lactic Acid chemistry, Voriconazole administration & dosage, Voriconazole pharmacology, Voriconazole chemistry, Antifungal Agents administration & dosage, Antifungal Agents pharmacology, Antifungal Agents chemistry, Candida albicans drug effects, Nanoparticles chemistry, Cyclosporine administration & dosage, Cyclosporine pharmacology, Cyclosporine chemistry, Drug Synergism, Biofilms drug effects, Polylactic Acid-Polyglycolic Acid Copolymer chemistry
- Abstract
The goal of this work is to investigate if the synergistic antifungal activity between cyclosporine A, CsA, and voriconazole, VRZ, increases when both drugs are encapsulated in a nanocarrier as compared when they are free. The preparation and characterization of blank and VRZ and CsA loaded polymeric based PLGA nanoparticles (PLGA, PLGA-PEG, and PLGA+PEG) was a necessary previous step. Using the more suitable NPs, those of PLGA, the antifungal susceptibility tests performed with VRZ-loaded PLGA NPs, show no significant increase of the antifungal activity in comparison to that of free VRZ. However, the synergistic behavior found for the (VRZ+CsA)-loaded PLGA NPs was fourfold stronger than that observed for the two free drugs together. On the other hand, the investigation into the suppression of C. albicans biofilm formation showed that blank PLGA NPs inhibit the biofilm formation at high NPs concentrations. However, a minor effect or even a slight biofilm increase formation was observed at low and moderate NPs concentrations. Therefore, the enhancement of the biofilm inhibition found for the three tested treatments (CsA alone, VRZ alone, and VRZ+CsA) when comparing free and encapsulated drugs, within the therapeutic window, can be attributed to the drug encapsulation approach. Indeed, polymeric PLGA NPs loaded with CsA, VRZ, or VRZ+CsA are more effective at inhibiting the C. albicans biofilm growth than their free counterparts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF