21 results on '"Li, Hua-Yu"'
Search Results
2. High myopia control is comparable between multifocal rigid gas-permeable lenses and spectacles
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Li-hua Yu, Ran Zhuo, Guan-xing Song, Meng Lin, and Wan-qing Jin
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axial length ,high myopia ,multifocal rigid gas-permeable lens ,myopia control ,lens design ,Medicine (General) ,R5-920 - Abstract
PurposeOcular pathology may be reduced by slowing myopia progression. The purpose of this study was to evaluate the potential of a novel custom-designed rigid gas permeable (RGP) contact lens to control high myopia by comparing the efficacy of multifocal RGP lenses and single-vision spectacles for high myopia control.MethodsThe medical records of children fitted with spectacles or multifocal rigid gas-permeable lenses between January 2018 and May 2020 were retrospectively reviewed. Children (5–17 years) with non-cycloplegic spherical equivalent refraction of ≤ −6.00 D or spherical equivalent refraction > − 6.00 D with baseline axial length ≥ 26.5 mm, and astigmatism of ≥ −2.00 D were included. Axial length and refraction were measured at baseline, before fitting the participants with multifocal rigid gas-permeable lenses or spectacles, and at 1- and 2-year follow-up visits. Changes in axial length were compared between the groups.ResultsAmong the 77 children with 1-year follow-up data, the mean axial elongation was 0.20 ± 0.17 mm and 0.21 ± 0.14 mm in the multifocal rigid gas-permeable and control groups, respectively, without significant differences between groups (F = 0.004, p = 0.835). Among the 41 patients who completed 2 years of follow-up, the mean axial elongation values in the multifocal rigid gas-permeable and control groups were 0.21 ± 0.15 mm and 0.24 ± 0.13 mm, respectively, at the 1-year follow-up, and 0.37 ± 0.27 mm and 0.43 ± 0.23 mm, respectively, at the 2-year follow-up, without significant between-group differences at either time point (p = 0.224).ConclusionAxial length increased at a similar rate in both the control (spectacles) and multifocal rigid gas-permeable lens groups, suggesting that multifocal rigid gas-permeable lenses have no significant impact on controlling high myopia progression compared with spectacles.
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- 2023
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3. OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets
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Yang, Zhi-Xue, primary, Deng, Dong-Hao, additional, Gao, Zhu-Ying, additional, Zhang, Zhi-Kang, additional, Fu, Ya-Wen, additional, Wen, Wei, additional, Zhang, Feng, additional, Li, Xiang, additional, Li, Hua-Yu, additional, Zhang, Jian-Ping, additional, and Zhang, Xiao-Bing, additional
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- 2024
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4. Differential Privacy Based Fingerprinting Obfuscation Mechanism Towards NetworkReconnaissance Deception
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HE Yuan, XING Chang-you, ZHANG Guo-min, SONG Li-hua, YU Hang
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fingerprinting obfuscation ,differential privacy ,network reconnaissance ,cyber deception defense ,Computer software ,QA76.75-76.765 ,Technology (General) ,T1-995 - Abstract
Network fingerprinting detection is an important network reconnaissance method,which can be used by attackers to obtain the fingerprinting characteristics of the target network,and then provide support for subsequent targeted attacks.Fingerprinting obfuscation technology enables attackers to form fake fingerprinting views by actively modifying the fingerprinting features in response packets.However,existing obfuscation methods are still insufficient in dealing with attackers’ strategic detection and analysis.To this end,a differential privacy based fingerprinting obfuscation mechanism(DPOF) towards network reconnaissance deception is proposed.Taking the idea of data privacy protection as a reference,DPOF first establishes a utility-driven differential privacy fingerprinting obfuscation model,and calculates the obfuscation probability of fake fingerprints with different utilities through the differential privacy exponential mechanism.On this basis,a fingerprinting obfuscation decision method under resource constraint is further designed,and an obfuscation strategy solving algorithm based on particle swarm optimization is implemented.Simulation results show that compared with the existing typical fingerprinting obfuscation methods,DPOF has better fingerprinting obfuscation effect with different problem scales and budgets,and can obtain a better approximate optimal strategy at a faster speed.
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- 2022
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5. Mesencephalic astrocyte-derived neurotrophic factor (MANF) prevents the neuroinflammation induced dopaminergic neurodegeneration
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Jing-Xing Zhang, Kai-Ge Zhou, Yan-Xin Yin, Ling-Jing Jin, Wei-Fang Tong, Jia Guo, Li-Hua Yu, Xian-Cheng Ye, and Ming Jiang
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Parkinson's disease (PD) ,Mesencephalic astrocyte-derived neurotrophic factor (MANF) ,Dopaminergic neurodegeneration ,Neuroinflammation ,Nrf-2 ,Microglia activation ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background: The excessive activation of the microglia leads to the release of inflammatory factors that contribute to neuronal cell loss and neurodegeneration in Parkinson's Disease (PD). Mesencephalic astrocyte-derived neurotrophic factor (MANF) that belongs to a newly found neurotrophic factors (NTFs) family has been reported to promote neuronal survival in the PD models. However, the effects of the MANF on neuroinflammation in PD remain unclear. Methods: AAV8-MANF virus was constructed to determine whether the high expression of MANF can protect the neuroinflammation-induced dopaminergic neurodegeneration in rats with 6-OHDA-induced PD. Rotarod performance test, immunofluorescent staining and western bolt were employed to evaluate the behavioral dysfunction, dopaminergic neurodegeneration, microglia activation, and signal activation. 6-OHDA treated SH-SY5Y cells and LPS treated BV-2 cells were used as the in vitro model for MANF neuroprotective and neuroinflammation mechanisms. Cell vitality and apoptosis were evaluated with MTT, CCK-8 and flow cytometric analysis. The AKT/GSK3β-Nrf2 signaling and the TNF-α/IL6 expression were measured by Western Blot. Results: Our findings indicated that the elevated MANF expression by the AAV8-MANF administration ameliorated the motor dysfunction and protected the dopaminergic neurons in the 6-OHDA treated rats. The upregulated CD11b in the rat SN caused by the 6-OHDA administration was significantly attenuated by the pretreatment of the AAV8-MANF. Furthermore, the levels of p-AKT, p-GSK3β, BCL-2, and Nrf-2 were upregulated by the high expression of the MANF. Under the oxidative stress of the 6-OHDA, the MANF significantly reduced the apoptotic effect of the TNF-α on the SH-SY5Y cells. In the LPS treated BV-2 cells, the MANF reduced the production of the TNF-α and IL-6, via enhancing the Nrf-2, p-Akt, p-GSK3β, and p-NF-κβ level. Conclusions: These results suggested that the MANF prevented the dopaminergic neurodegeneration caused by the microglia activation in PD via activation of the AKT/GSK3β-Nrf-2 signaling axis.
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- 2023
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6. Common key ingredient concluded in Baitouweng decoction, GegenQinliandecoction and Shaoyao decoction prevents radiation enteritis by regulatinggutmicrobial network.
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Tian-hao Liu, Ying-yue Sheng, Chen-yang Zhang, Hang Zhang, Xue Li, Li-hua Yu, Ling-ling Xu, Lin Zhao, Hong Wei, Ya-hong Zhou, and Yu-zheng Xue
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ENTERITIS ,GUT microbiome ,HERBAL medicine ,QUERCETIN ,LIQUID chromatography - Abstract
Background: The gut microbiota is crucial in the process of radiation enteritis (RE). Asrecommended by Chinese expert consensus, three formulas such as Baitouweng decoction, Gegen Qinlian decoction, and Shaoyao decoction, are used to prevent the same type of RE. Methods: Herein, we aimed to investigate the common pharmaceutical ingredients andmolecular mechanisms of the three formulas on RE from the perspective of gut microbiotathrough multi-bioinformatics analysis and mouse experiment. Results: Interestingly, theimportant active ingredient screened was quercetin in all three herbal formulas, andthedominant targets that participated in RE treatment by targeting gut microbiota regulationwere IL10, STAT1, IL1B, and IL1A. The concentration of quercetin was measured inGegenQinlian decoction > Shaoyao decoction > Baitouweng decoction (P <0.001) usinghighperformance liquid chromatography. Moreover, a total of 40 SPF C57/BL6J (male, 12–14weeks old) mice were assigned to perform the validation experiment with various quercetinintervention doses. Quercetin significantly protected against inflammatory responseinradiation induced-mice. The microbial alterations in the gut caused by quercetinintervention in radiation-induced mice were also explained. We further confirmedthealterations in the relative mRNA expression of the three formulas for the treatment of RE, which have four similar gut microbiota-regulated targets (IL10, STAT1, IL1B, andIL1A). Conclusion: These findings show that quercetin regulated the gut microbiota for REtherapy, a connotation that works on the principle of “different treatments for the samedisease” for RE. The present investigation provides methodological guidelines and protocolsfor the modern use and examination of traditional therapeutic concepts. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Convergence map with action-angle variables based on square matrix for nonlinear lattice optimization
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Li Hua Yu, Yoshiteru Hidaka, Victor Smaluk, Kelly Anderson, and Yue Hao
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Nuclear and particle physics. Atomic energy. Radioactivity ,QC770-798 - Abstract
To analyze nonlinear dynamic systems, we developed a new technique based on the square matrix method. We propose this technique called the “convergence map” for generating particle stability diagrams similar to the frequency maps widely used in accelerator physics to estimate dynamic aperture. The convergence map provides similar information as the frequency map but in a much shorter computing time. The dynamic equation can be rewritten in terms of action-angle variables provided by the square matrix derived from the accelerator lattice. The convergence map is obtained by solving the exact nonlinear equation iteratively by the perturbation method using Fourier transform and studying convergence numerically. When the iteration is convergent, the solution is expressed as a quasiperiodic analytical function as a highly accurate approximation and hence the motion is stable. The border of stable motion determines the dynamical aperture. As an example, we applied the new method to the nonlinear optimization of the NSLS-II storage ring and demonstrated a dynamic aperture comparable to or larger than the nominal one obtained by particle tracking. The computation speed of the convergence map is 30 to 300 times faster than the speed of the particle tracking, depending on the size of the ring lattice (number of superperiods). The computation speed ratio is larger for complex lattices with low symmetry, such as particle colliders.
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- 2023
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8. Convergence map with action-angle variables based on square matrix for nonlinear lattice optimization.
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Li Hua Yu, Yoshiteru Hidaka, and Victor Smaluk
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- 2022
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9. The Up-regulation of TNF-α Maintains Trigeminal Neuralgia by Modulating MAPKs Phosphorylation and BKCa Channels in Trigeminal Nucleus Caudalis
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Zhan-ying Lu, Juan Fan, Li-hua Yu, Bei Ma, and Li-ming Cheng
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trigeminal neuralgia ,TNF-α ,trigeminal nucleus caudalis ,MAPKs phosphorylation ,BKCa channel ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Trigeminal neuralgia (TN) is a severe chronic neuropathic pain. Despite numerous available medical interventions, the therapeutic effects are not ideal. To control the pain attacks, the need for more contemporary drugs continues to be a real challenge. Our previous study reported that Ca2+-activated K+ channels (BKCa) channels modulated by mitogen-activated protein kinases (MAPKs) in the trigeminal ganglia (TG) neurons play crucial roles in regulating TN, and some research studies demonstrated that inflammatory cytokine tumor necrosis factor alpha (TNF-α) could promote neuropathic pain. Meanwhile, the trigeminal nucleus caudalis (TNC), the first central site of the trigeminal nociceptive pathway, is responsible for processing sensory and pain signals from the peripheral orofacial area. Thus, this study is aimed to further investigate whether TNF-α and MAPKs phosphorylation in the TNC could mediate the pathogenesis of TN by modulating BKCa channels. The results showed that TNF-α of the TNC region is upregulated significantly in the chronic constriction injury of infraorbital nerve (ION-CCI) rats model, which displayed persistent facial mechanical allodynia. The normal rats with target injection of exogenous TNF-α to the fourth brain ventricle behaved just like the ION-CCI model rats, the orofacial mechanical pain threshold decreased clearly. Meanwhile, the exogenous TNF-α increased the action potential frequency and reduced the BKCa currents of TNC neurons significantly, which could be reversed by U0126 and SB203580, the inhibitors of MAPK. In addition, U0126, SB203580, and another MAPK inhibitor SP600125 could relieve the facial mechanical allodynia by being injected into the fourth brain ventricle of ION-CCI model rats, respectively. Taken together, our work suggests that the upregulation of TNF-α in the TNC region would cause the increase of MAPKs phosphorylation and then the negative regulation of BKCa channels, resulting in the TN.
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- 2021
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10. A comment on “A multicenter randomized-controlled trial of nucleos(t)ide analogue cessation in HBeAg-negative chronic hepatitis B.” (J Hepatol. 2023 May;78(5):926-936.)
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Wan, Yue-Meng, primary, Wu, Hua-Mei, additional, Huang, Song-Quan, additional, Li, Hua-Yu, additional, and Yin, Hong-Jing, additional
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- 2023
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11. Ceramic particle-induced accelerated solution-aging behavior of spray-formed 7055 aluminum alloy TIG weld metal
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Yun Cheng, Wei-xia Yuan, Jun-hua Xu, Li-hua Yu, Yun-xuan Hu, Ting Huang, and Hao Zhang
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science - Published
- 2023
12. Synthesis and Evaluation of Novel Nitric Oxide-Donating Ligustrazine Derivatives as Potent Antiplatelet Aggregation Agents
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Li, Han-Xu, primary, Tian, Jian-Hui, additional, Li, Hua-Yu, additional, Wan, Xin, additional, and Zou, Yu, additional
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- 2023
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13. Study on Preparation and Properties of PPB/POE/Calcite Waste Powder Composites.
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MA Lan-yu, LAI Wen-qin, XIE Song-bo, LI Hua-yu, CHEN Zhao-lun, and HUANG Yi-feng
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CALCITE ,IMPACT strength ,THERMAL properties ,POWDERS ,TEMPERATURE effect ,ELASTOMERS ,THERMOPLASTIC elastomers - Abstract
In order to realize the resource utilization of calcite tailings corner waste, block copolymerized polypropylene (PPB)/polyolefin elastomer (POE)/calcite waste powder ternary composites were prepared by melt blending modified extrusion method. The effects of waste powder content and POE content on mechanical properties, thermal properties and microstructure of composites were investigated. The results show that POE can improve compatibility of composites, and calcite waste powder can significantly enhance stiffness. Adding 10 phr of calcite waste powder and 20 phr of POE to the PPB base material can improve compatibility between inorganic filler and resin substrate and significantly improve mechanical properties of composites. The impact strength of composites is increased to 55.48 kJ/m2 by the coordination of calcite waste powder and POE. POE and calcite waste powder both reduce the crystallinity of composites, have little effect on melting temperature, and slightly increase crystallization temperature. PPB, POE and calcite waste powder are compounded to prepare composites, which provided a new way to solve the application problem of calcite tailings. [ABSTRACT FROM AUTHOR]
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- 2023
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14. A comment on “A multicenter randomized-controlled trial of nucleos(t)ide analogue cessation in HBeAg-negative chronic hepatitis B”
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Wan, Yue-Meng, Wu, Hua-Mei, Huang, Song-Quan, Li, Hua-Yu, and Yin, Hong-Jing
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- 2024
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15. [Post-stroke shoulder-hand syndrome of phlegm-stasis obstruction treated with the combined therapy of eye acupuncture
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Mei, Wang, Peng-Qin, Wang, Li-Hua, Yu, Chen-Yang, Wang, and Yan, Shao
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Reflex Sympathetic Dystrophy ,Stroke ,Treatment Outcome ,Acupuncture Therapy ,Stroke Rehabilitation ,Humans ,Pain ,Acupuncture Points - Abstract
To assess the efficacy on relieving pain and improving the range of motion of shoulder joint in post-stroke shoulder-hand syndrome of phlegm-stasis obstruction in treatment of the combined therapy of eye acupuncture,A total of 356 patients with post-stroke shoulder-hand syndrome of phlegm-stasis obstruction were randomized into an eye acupuncture +The scores of VAS, GEPI and NIHSS were all improved with the treatment lasting in the three groups (The combined therapy of eye acupuncture
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- 2022
16. Defect-engineered surfaces to investigate the formation of self-assembled molecular networks
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Li-Hua Yu, Zhen-Feng Cai, Lander Verstraete, Yuanzhi Xia, Yuan Fang, Louis Cuccia, Oleksandr Ivasenko, and Steven De Feyter
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GRAPHENE ,Science & Technology ,GRAPHITE ,DIAZONIUM CHEMISTRY ,IMPACT ,Chemistry, Multidisciplinary ,COOPERATIVITY ,ORGANIZATION ,General Chemistry ,INTERFACE ,Chemistry ,POLYMERIZATION ,Physical Sciences ,COVALENT FUNCTIONALIZATION ,CORRALS - Abstract
Herein we report the impact of covalent modification (grafting), inducing lateral nanoconfinement conditions, on the self-assembly of a quinonoid zwitterion derivative into self-assembled molecular networks at the liquid/solid interface. At low concentrations where the compound does not show self-assembly behaviour on bare highly oriented pyrolytic graphite (HOPG), close-packed self-assembled structures are visualized by scanning tunneling microscopy on covalently modified HOPG. The size of the self-assembled domains decreases with increasing the density of grafted molecules, i.e. the molecules covalently bound to the surface. The dynamics of domains are captured with molecular resolution, revealing not only time-dependent growth and shrinkage processes but also the orientation conversion of assembled domains. Grafted pins play a key role in initiating the formation of on-surface molecular self-assembly and their stabilization, providing an elegant route to study various aspects of nucleation and growth processes of self-assembled molecular networks., Chemical Science, 13 (44), ISSN:2041-6520, ISSN:2041-6539
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- 2022
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17. Class 2 Heating Cycles: A New Class of Thermodynamic Cycles
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Li, Hua-Yu, primary and Li, Hong-Rui, additional
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- 2021
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18. Network Pharmacology-Based and Molecular Docking-Based Analysis of Suanzaoren Decoction for the Treatment of Parkinson's Disease with Sleep Disorder
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Xin-xiang Zhang, Yan-yun Liu, Li-hua Yu, Jia-ming Yu, Dao-jun Xie, and Juan Zhang
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Sleep Wake Disorders ,Parkinson's disease ,Article Subject ,Computational biology ,Biology ,Network Pharmacology ,General Biochemistry, Genetics and Molecular Biology ,Antioxidants ,GeneCards ,medicine ,Humans ,KEGG ,Kaempferols ,Medicine, Chinese Traditional ,General Immunology and Microbiology ,Parkinson Disease ,General Medicine ,AutoDock ,medicine.disease ,Molecular Docking Simulation ,Mechanism of action ,Medicine ,Quercetin ,UniProt ,Signal transduction ,medicine.symptom ,Systems pharmacology ,Drugs, Chinese Herbal ,Signal Transduction ,Research Article - Abstract
This study is aimed at exploring the possible mechanism of action of the Suanzaoren decoction (SZRD) in the treatment of Parkinson’s disease with sleep disorder (PDSD) based on network pharmacology and molecular docking. Traditional Chinese Medicine Systems Pharmacology (TCMSP) was used to screen the bioactive components and targets of SZRD, and their targets were standardized using the UniProt platform. The disease targets of “Parkinson’s disease (PD)” and “Sleep disorder (SD)” were collected by OMIM, GeneCards, and DisGeNET databases. Thereafter, the protein-protein interaction (PPI) network was constructed using the STRING platform and visualized by Cytoscape (3.7.2) software. Then, the DAVID platform was used to analyze the Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Cytoscape (3.7.2) software was also used to construct the network of the “herb-component-target-pathway.” The core active ingredients and core action targets of the drug were verified by molecular docking using AutoDock software. A total of 135 Chinese herbal components and 41 corresponding targets were predicted for the treatment of PDSD using SZRD. Fifteen important signaling pathways were screened, such as the cancer pathway, TNF signaling pathway, PI3K-AKT signaling pathway, HIF-1 signaling pathway, and Toll-like receptor signaling pathway. The results of molecular docking showed that the main active compounds could bind to the representative targets and exhibit good affinity. This study revealed that SZRD has the characteristics and advantages of “multicomponent, multitarget, and multipathway” in the treatment of PDSD; among these, the combination of the main active components of quercetin and kaempferol with the key targets of AKT1, IL6, MAPK1, TP53, and VEGFA may be one of the important mechanisms. This study provides a theoretical basis for further study of the material basis and molecular mechanism of SZRD in the treatment of PDSD.
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- 2021
19. Class 1 Heating Cycles: A New Class of Thermodynamic Cycles
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Li, Hong-Rui, primary and Li, Hua-Yu, additional
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- 2021
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20. Enhancing the stability and electrocatalytic activity of Ti-based PbO2 anodes by introduction of an arc-sprayed TiN interlayer
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Changbin Tang, Li-Hua Yu, Duanduan Cui, Juanqin Xue, Xiangyang Yin, and Liu Zilong
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Materials science ,General Chemical Engineering ,chemistry.chemical_element ,engineering.material ,Anode ,Surface coating ,Coating ,Chemical engineering ,chemistry ,Electrode ,Electrochemistry ,engineering ,Surface roughness ,Surface layer ,Tin ,Titanium - Abstract
Ti/TiN/PbO2 electrodes were prepared using an arc-sprayed TiN interlayer on Ti substrates, followed by the anodic electrodeposition of a PbO2 catalytic surface layer on it. In order to investigate the positive effect of the TiN interlayer on the stability and electrocatalytic activity of the anodes, microstructural, surface roughness, adhesion evaluation, accelerated life and electrochemical performance tests and electrooxidation experiments of phenol simulated wastewater were conducted and compared with Ti/PbO2 anodes without the interlayer. The experimental results demonstrated that the arc-sprayed TiN interlayer with a thickness of 140–180 μm had a higher surface roughness and hardness than that of its titanium substrate. The coatings forming the electrode were firmly bonded to the substrate. The conductivity of the Ti-based PbO2 anode containing the TiN interlayer was significantly improved. The PbO2 surface coating achieved a faster deposition rate and finer grains, which produce a number of anode active sites and the electrocatalytic performance was boosted with a removal rate of phenol of up to 85.2%, which was higher than that of Ti/PbO2 by 30.6%. Simultaneously, the anode stability was also significantly enhanced, i.e., the Ti/TiN/PbO2 anode constructed by introducing the TiN interlayer exhibited excellent stability and electrocatalytic performance. The high-performance anode obtained relied not only on the presence of the arc-sprayed TiN interlayer, which significantly improved the overall physicochemical properties of the electrode, but also on the kinetic rate of the electrocrystallization growth of the refined PbO2 coating that was accelerated.
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- 2021
21. Estrogen receptor β alleviates inflammatory lesions in a rat model of inflammatory bowel disease via down-regulating P2X7R expression in macrophages
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Bei Ma, Yuchen Liu, Wenxin Li, Zhan-ying Lu, Xu Zhu, Li-ming Cheng, Qian Jiang, and Li-hua Yu
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MAPK/ERK pathway ,Janus kinase 2 ,biology ,Chemistry ,Kinase ,Macrophages ,Estrogen receptor ,Inflammation ,Cell Biology ,Pharmacology ,Colitis ,CREB ,Biochemistry ,Rats ,Mice ,RAW 264.7 Cells ,biology.protein ,medicine ,Animals ,Estrogen Receptor beta ,medicine.symptom ,STAT3 ,Estrogen receptor beta - Abstract
Estrogen receptor beta (ERβ) agonists could inhibit inflammation in animal models of inflammatory bowel disease (IBD). However, the mechanism underlying such effect and the potential role of P2 × 7 receptor (P2X7R) remains unclear. In the present study, we examined whether the effect of ERβ activation in IBD rats was related to P2X7R. Overexpression of ERβ using a recombinant lentivirus in IBD rats improved the IBD-like symptoms, including weight loss, disease activity index (DAI) scores, and inflammatory responses. ERβ agonists DPN and ERB-041 attenuated P2X7R expression in macrophages from colitis rats and in a murine macrophage cell line (RAW264.7) in response to either lipopolysaccharide (LPS) or adenosine triphosphate (ATP). DPN and ERB-041 also blocked increased production of TNF-α, IL-6, and IL-1β in the rectocolon of colitis rats. The two ERβ agonists reversed LPS- and ATP-induced up-regulation of P2X7R and its downstream proteins, including NLRP3, ASC, caspase-1, and pro-IL-1β, in RAW264.7 cells. Also, in both the rectocolon of colitis rats and RAW264.7 cells, ERβ agonists reversed the up-regulation of extracellular regulated protein kinases (ERK), the Janus kinase 2 (JAK2), and signal transducer and activator of transcription 3 (STAT3), but not up-regulation of serine threonine kinase or cAMP-response element binding protein (CREB). Blockade of JAK2 or STAT3 phosphorylation significantly reduced the ability of DPN to down-regulate P2X7R expression and the ability of ERB-041 and DPN to inhibit IL-1β release from RAW264.7 cells. We found that ERβ and P2X7R co-localized in the macrophages of rat rectocolon and in RAW264.7 cells. Deletion of macrophages from colitis rats with clodronate abolished the inhibitory effect of DPN. These results suggest that ERβ plays an important anti-inflammatory role in IBD rats by down-regulating P2X7R expression and inhibiting IL-1β release from macrophages through the JAK2/STAT3 signaling pathway.
- Published
- 2021
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