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181 results on '"Li, Shujiang"'

8. Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology

Author

Wang Shiwei, Tan Yu, Li Shujiang, and Zhu Tianhui

Subjects
dynamic analysis, high throughput sequencing, leaf-associated fungi, spot disease, walnut, Genetics, QH426-470, Microbiology, QR1-502
Abstract

Leaf-associated microbiota is vital in plant-environment interactions and is the basis for micro-ecological regulation. However, there are no studies on the direct differences in microbial community composition between disease-susceptible and healthy walnut leaves. This study collected five samples of healthy and infected leaves (all leaves with abnormal spots were considered diseased leaves) from May to October 2018. Differences in fungal diversity (Chao1 index, Shannon index, and Simpson index) and community structure were observed by sequencing and analyzing diseased and healthy leaf microbial communities by Illumina HiSeq sequencing technology. The main fungal phyla of walnut leaf-associated were Ascomycota, Basidiomycota, and Glomeromycota. Diversity indices (Shannon and Chao1 index values) of healthy leaves differed significantly in the late stages of disease onset. The results showed that the fungal species that differed considerably between the healthy and infected groups differed, and the fungal species that differed significantly between the healthy and infected groups changed with the development of the leaf disease. Critical control time points were determined by analyzing the population dynamics of pathogenic fungi. Leaf-associated microorganisms are abundant and diverse, and fungal identification and diversity studies are helpful for developing more appropriate walnut management strategies

Published
2022
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15. Field measurements of turbulent mixing south of the Lombok Strait, Indonesia.

Author

Susanto, R. Dwi, Wei, Zexun, Santoso, Priyadi Dwi, Wang, Guanlin, Fadli, Muhammad, Li, Shujiang, Agustiadi, Teguh, Xu, Tengfei, Priyono, Bayu, Li, Ying, and Fang, Guohong

Subjects
TURBULENT mixing, OCEAN circulation, SEAWATER, WATER masses, ENERGY dissipation
Abstract

The Indonesian seas, with their complex passages and vigorous mixing, constitute the only route and are critical in regulating Pacific–Indian Ocean interchange, air–sea interaction, and global climate events. Previous research employing remote sensing and numerical simulations strongly suggested that this mixing is tidally driven and localized in narrow channels and straits, with only a few direct observations to validate it. The current study offers the first comprehensive temporal microstructure observations in the south of Lombok Strait with a radius of 0.05° and centered on 115.54oE and 9.02oS. Fifteen days of tidal mixing observations measured potential temperature and density, salinity, and turbulent energy dissipation rate. The results revealed significant mixing and verified the remotely sensed technique. The south Lombok temporal and depth averaged of the turbulent kinetic energy dissipation rate, and the diapycnal diffusivity from 20 to 250 m are ε = 4.15 ± 15.9) × 10–6 W kg–1 and K ρ = (1.44 ± 10.7) × 10–2 m2s–1, respectively. This K ρ is up to 104 times larger than the Banda Sea [ K ρ = (9.2 ± 0.55) × 10–6 m2s–1] (Alford et al. Geophys Res Lett 26:2741–2744, 1999) or the "open ocean" K ρ = 0.03 × 10–4 m2s−1 within 2° of the equator to (0.4–0.5) × 10–4 m2s−1 at 50°–70° (Kunze et al. J Phys Oceanogr 36:1553–1576, 2006). Therefore, nonlinear interactions between internal tides, tidally induced mixing, and ITF plays a critical role regulating water mass transformation and have strong implications to longer-term variations and change of Pacific–Indian Ocean water circulation and climate. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Activities of cell‐wall‐degrading enzymes produced by Pestalotiopsis guepinii.

Author

Wu, Sijun, Qiao, Tianmin, Li, Shujiang, Hu, Binhong, Zhu, Hanmingyue, and Zhu, Tianhui

Subjects
PESTALOTIOPSIS, CELLULASE, ENZYMES, XYLANASES, PATHOGENIC fungi, BIOCHEMICAL substrates, ENZYME kinetics
Abstract

Pestalotiopsis guepinii is a pathogenic fungus that causes grey blight on Camellia pitardii. In this study, we investigated the enzyme activity and kinetics of these cell‐wall‐degrading enzymes (CWDEs) produced by Pestalotiopsis guepinii in both C. pitardii leaves and culture medium. Our enzyme activity experiments revealed that the activities of xylanase, pectin methyl‐galacturonase (PMG), β‐1,4‐endoglucanase (Cx), and β‐glucosidase were high in both C. pitardii leaves and culture medium. These enzymes played a significant role in the pathogenic process. However, the activity of laccase was found to be very low and had a minor impact on the pathogenic process. Furthermore, our enzyme dynamics experiments demonstrated that the optimal reaction temperature for PMG and Cx was 50°C, while for β‐glucosidase and xylanase, it was 60°C. The optimal reaction pH for Cx, β‐glucosidase, and xylanase was 5.0, whereas for PMG, it ranged from 5.0 to 6.0. This indicates that these four enzymes prefer acidic conditions. Moreover, we observed that the activities of Cx, PMG, and xylanase decreased with increasing reaction time. On the other hand, the activity of β‐glucosidase initially increased sharply and then decreased slowly. The maximum reaction rates of the four cell‐wall‐degrading enzymes were ranked as follows: xylanase > PMG > β‐glucosidase > Cx. Additionally, the affinities of these enzymes with substrates were ranked as follows: PMG < Cx < xylanase < β‐glucosidase. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Colletotrichum fructicola, Causal Agent of Shot-Hole Symptoms on Leaves of Prunus sibirica in China

Author

Han, Shan, primary, Xu, Xiu, additional, Jiang, Yaorong, additional, Zheng, Zhenglei, additional, Yuan, Huan, additional, Li, Shujiang, additional, Liu, Yinggao, additional, Lin, Tiantian, additional, Qiao, Tianmin, additional, Yang, Chunlin, additional, Li, Shuying, additional, Dong, JiaXin, additional, Fang, Haonan, additional, and Zhu, Tianhui, additional

Published
2023
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31. Nigrospora musae Causing Taxus chinensis var. mairei stem blight in Sichuan, China

Author

Chen, Xingyu, primary, Xu, Xiu, additional, Yuan, Huan, additional, Luo, Tingting, additional, Cui, Hui, additional, Zhu, Tianhui, additional, Li, Shujiang, additional, Liu, Yinggao, additional, Lin, Tiantian, additional, Qiao, Tianmin, additional, Yang, Chunlin, additional, Li, Shuying, additional, Xie, Liling, additional, Dong, Jiaxin, additional, and Han, Shan, additional

Published
2023
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32. The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis.

Author

Yan, Peng, Wang, Yisi, Yu, Cailin, Piao, Jingmei, Li, Shuying, Liu, Yinggao, and Li, Shujiang

Subjects
GENETIC overexpression, PLANT genetic transformation, GENE expression, TRANSGENIC plants, DISEASE incidence, BLIGHT diseases (Botany)
Abstract

Arthrinium phaeospermum is the major pathogen responsible for the significant stem disease "blight" in B. pervariabilis × D. grandis. The interacting proteins of the key pathogenic factor ApCtf1β, BDUbc and BDSKL1, have previously been obtained by two-hybrid, BiFC, GST pull-down yeast assays. However, the functions of these interacting proteins remain unknown. This study successfully obtained transgenic plants overexpressing BDUbc, BDSKL1, and BDUbc + BDSKL1 via Agrobacterium-mediated gene overexpression. qRT-PCR analysis revealed significantly increased expression levels of BDUbc and BDSKL1 in the transgenic plants. After infection with the pathogenic spore suspension, the disease incidence and severity index significantly decreased across all three transgenic plants, accompanied by a marked increase in defense enzyme levels. Notably, the co-transformed plant, OE-BDUbc + BDSKL1, demonstrated the lowest disease incidence and severity index among the transgenic variants. These results not only indicate that BDUbc and BDSKL1 are disease-resistant genes, but also that these two genes may exhibit a synergistic enhancement effect, which further improves the resistance to blight in Bambusa pervariabilis × Dendrocalamopsis grandis. [ABSTRACT FROM AUTHOR]

Published
2024
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33. Leaf Spot on Alocasia macrorrhizos Caused by Fusarium asiaticum in Sichuan, China

Author

Zheng, Zhenlei, primary, Li, Yanyue, additional, Wang, Ming, additional, Ruan, Ruoyu, additional, Yang, Bin, additional, Zhu, Tianhui, additional, Li, Shujiang, additional, Liu, Yinggao, additional, Qiao, Tianmin, additional, Yang, Chunlin, additional, Lin, Tiantian, additional, Huang, Qiong, additional, Li, Shuying, additional, and Han, Shan, additional

Published
2023
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49. Decadal Weakening of Abyssal South China Sea Circulation

Author

Zhu, Yaohua, primary, Yao, Jingxin, additional, Li, Shujiang, additional, Xu, Tengfei, additional, Huang, Rui Xin, additional, Nie, Xunwei, additional, Pan, Haidong, additional, Wang, Yonggang, additional, Fang, Yue, additional, and Wei, Zexun, additional

Published
2022
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