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Introduction: Over the past two decades, Esports has emerged as a prominent competitive field. With increasing levels of competition and the professionalization of events, the performance skills of Esports athletes have become a critical area of research. This study aimed to systematically review the academic literature on Esports athletes' performance skills from the past decade and validate these findings through interviews with experienced Esports practitioners. Methods: The first phase of this study involved a systematic literature review to collect and screen academic journals from the past decade related to Esports athletes' performance skills, from which key skills were identified. In the second phase, in-depth interviews were conducted with ten experienced domestic Esports practitioners. The interview data were analyzed and compared to the literature review findings using the established framework from the literature. Results: The systematic literature review yielded 46 relevant articles, from which 14 performance-related skills were identified. Classification of these skills based on the literature indicated that recent studies primarily focus on the psychological skills of Esports athletes, with a particular emphasis on self-regulation and inhibitory control skills, such as focus and stress adaptation. The interview results corroborated the skills identified in the literature review. Practitioners also highlighted the importance of hand-eye coordination/reaction time, cognitive flexibility/spatial cognition, stress adaptation, and teamwork as critical capabilities in Esports. Conclusion: The field of Esports performance skills represents a growing and interdisciplinary area of study, with many research questions yet to be explored. The findings from this systematic literature review and practitioner interviews provide a foundation for future research and practical applications by scholars and industry professionals. [ABSTRACT FROM AUTHOR]