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Accurately evaluating the 3-D profile of nonferromagnetic metal surface defects is essential for nondestructive evaluation. Eddy current pulsed thermography (ECPT) has been used for reconstructing surface defects, which utilizes multiphysics coupling of electromagnetic and thermal fields. Previous investigations mainly employ the thermal field for reconstruction. However, the thermal field varies slowly over time and has weak correlation, which causes serious blur and noise. In this article, a surface defect 3-D profile reconstruction method based on eddy current field distribution is proposed. The proposed method takes advantage of the strong global correlation of eddy current fields and proposes an inversion technique to reconstruct defects. Experimental results demonstrate that the proposed method can effectively reconstruct the 3-D profile of defects with depths within the skin depth, including complex-shaped defects and natural cracks.

Adenosylhomocysteinase (AHCY), a key enzyme in the methionine cycle, is essential for the development of embryos and the maintenance of mouse embryonic stem cells (mESCs). However, the precise underlying mechanism of Ahcyin regulating pluripotency remains unclear. As the only enzyme that can hydrolyze S-adenosylhomocysteine in mammals, AHCY plays a critical role in the metabolic homeostasis, epigenetic remodeling, and transcriptional regulation. Here, we identified Ahcyas a direct target of OCT4 and unveiled that AHCY regulates the self-renewal and differentiation potency of mESCs through multiple mechanisms. Our study demonstrated that AHCY is required for the metabolic homeostasis of mESCs. We revealed the dual role of Ahcyin both transcriptional activation and inhibition, which is accomplished via the maintenance of H3K4me3 and H3K27me3, respectively. We found that Ahcyis required for H3K4me3-dependent transcriptional activation in mESCs. We also demonstrated that AHCY interacts with polycomb repressive complex 2 (PRC2), thereby maintaining the pluripotency of mESCs by sustaining the H3K27me3-regulated transcriptional repression of related genes. These results reveal a previously unrecognized OCT4–AHCY–PRC2 axis in the regulation of mESCs’ pluripotency and provide insights into the interplay between transcriptional factors, cellular metabolism, chromatin dynamics and pluripotency regulation.Our results reveal a previously unrecognized OCT4–AHCY–PRC2 axis in the regulation of mESCs’ pluripotency and shed light on the interplay between transcriptional factors, cellular metabolism, chromatin dynamics and pluripotency regulation.Graphical Abstract

Eddy current pulsed thermography (ECPT) has attracted much attention in nondestructive testing for its noncontact and large field of view. However, the ECPT images usually suffer from the thermal diffusion blurs. The fusion of temperature spatial and temporal features is the hotspot in the new research of ECPT enhancing methods, but these two features are contradictory on the speed and the accuracy of algorithms. Specifically, spatial features process fast but perform poorly in accuracy and antinoise ability, while temporal features are usually calculated from the whole ECPT video sequence, which inevitably increases the demand for data storage and the time cost, especially in the detection of large workpieces, such as engine blades or pressure pipelines. In this article, an enhanced diffusion-based method (EDBM) is proposed to solve this issue, which maps the temporal features through the spatial features of a single ECPT image, significantly reduces the input data volume and shows great potential in ECPT online detection. Experiments on multiple artificial and natural samples verify that, compared with raw ECPT image, the proposed EDBM can reduce root-mean-square error by 51.7%–86.5% (73.2% on average) and improve signal-to-noise ratio by 3.70–16.8 times (6.82 times on average), which performs better than the commonly spatial-based and temporal-based ECPT enhancement algorithms, such as enhanced Canny and independent component analysis, close to the latest sparse-model decomposition methods, but with two orders of magnitude less time cost.

Nonferromagnetic metal materials are widely used in industry. Defects generated during manufacture and use may lead to serious accidents. The defect reconstruction is important for nondestructive evaluation. Eddy current pulsed thermography (ECPT) is a well-known nondestructive testing and evaluation method, but hardly reconstruct fully defect profile. Electrical impedance tomography (EIT) shows promising potential in defect profile reconstruction, but suffers from electrode number limitation. In this article, EIT is introduced to ECPT image sequences processing, and a new method, induced current thermo-electrical impedance tomography, is developed. The proposed method takes each infrared camera pixel as a virtual electrode, captures the electric current distribution with spatial resolution as high as camera, and reconstructs conductivity distribution at pixel level from which the defect profile can be identified. Experiments with different defects are carried out to verify the performance of the proposed method.