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15. Model-assisted analysis for tuning anthocyanin composition in grape berries

Author

Wang, Yongjian, primary, Shang, Boxing, additional, Génard, Michel, additional, Hilbert-Masson, Ghislaine, additional, Delrot, Serge, additional, Gomès, Eric, additional, Poni, Stefano, additional, Keller, Markus, additional, Renaud, Christel, additional, Kong, Junhua, additional, Chen, Jinliang, additional, Liang, Zhenchang, additional, and Dai, Zhanwu, additional

Published
2023
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17. The Genome of Vitis zhejiang-adstricta Strengthens the Protection and Utilization of the Endangered Ancient Grape Endemic to China.

Author

Li, Huayang, Liu, Yongbo, Fan, Peige, Dai, Zhanwu, Hao, Jiachen, Duan, Wei, Liang, Zhenchang, and Wang, Yi

Subjects
VITIS vinifera, GENOMES, GENE families, NATURAL immunity, METABOLISM, ENDANGERED plants
Abstract

Vitis zhejiang-adstricta (V. zhejiang-adstricta) is one of the most important and endangered wild grapes. It is a national key protected wild, rare and endangered ancient grape endemic to China and used as a candidate material for resistance breeding owing to its excellent significant disease resistance. Here, we present a high-quality chromosome-level assembly of V. zhejiang-adstricta (IB-VB-01), comprising 506.66 Mb assembled into 19 pseudo-chromosomes. The contig N50 length is 3.91 Mb with 31,196 annotated protein-coding genes. Comparative genome and evolutionary analyses illustrated that V. zhejiang-adstricta has a specific position in the evolution of East Asian Vitis and shared a common ancestor with Vitis vinifera during the divergence of the two species about 10.42 (between 9.34 and 11.12) Mya. The expanded gene families compared with those in plants were related to disease resistance, and constructed gene families were related to plant growth and primary metabolism. With the analysis of gene family expansion and contraction, the evolution of environmental adaptability and especially the NBS-LRR gene family of V. zhejiang-adstricta was elucidated based on the pathways of resistance genes (R genes), unique genes and structural variations. The near-complete and accurate diploid V. zhejiang-adstricta reference genome obtained herein serves as an important complement to wild grape genomes and will provide valuable genomic resources for investigating the genomic architecture of V. zhejiang-adstricta as well as for improving disease resistance breeding strategies in grape. [ABSTRACT FROM AUTHOR]

Published
2024
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18. VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape

Author

Liu, Wenwen, primary, Mu, Huayuan, additional, Yuan, Ling, additional, Li, Yang, additional, Li, Yuting, additional, Li, Shenchang, additional, Ren, Chong, additional, Duan, Wei, additional, Fan, Peige, additional, Dai, Zhanwu, additional, Zhou, Yongfeng, additional, Liang, Zhenchang, additional, Li, Shaohua, additional, and Wang, Lijun, additional

Published
2023
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21. Dual domestications and origin of traits in grapevine evolution

Author

Dong, Yang, primary, Duan, Shengchang, additional, Xia, Qiuju, additional, Liang, Zhenchang, additional, Dong, Xiao, additional, Margaryan, Kristine, additional, Musayev, Mirza, additional, Goryslavets, Svitlana, additional, Zdunić, Goran, additional, Bert, Pierre-François, additional, Lacombe, Thierry, additional, Maul, Erika, additional, Nick, Peter, additional, Bitskinashvili, Kakha, additional, Bisztray, György Dénes, additional, Drori, Elyashiv, additional, De Lorenzis, Gabriella, additional, Cunha, Jorge, additional, Popescu, Carmen Florentina, additional, Arroyo-Garcia, Rosa, additional, Arnold, Claire, additional, Ergül, Ali, additional, Zhu, Yifan, additional, Ma, Chao, additional, Wang, Shufen, additional, Liu, Siqi, additional, Tang, Liu, additional, Wang, Chunping, additional, Li, Dawei, additional, Pan, Yunbing, additional, Li, Jingxian, additional, Yang, Ling, additional, Li, Xuzhen, additional, Xiang, Guisheng, additional, Yang, Zijiang, additional, Chen, Baozheng, additional, Dai, Zhanwu, additional, Wang, Yi, additional, Arakelyan, Arsen, additional, Kuliyev, Varis, additional, Spotar, Gennady, additional, Girollet, Nabil, additional, Delrot, Serge, additional, Ollat, Nathalie, additional, This, Patrice, additional, Marchal, Cécile, additional, Sarah, Gautier, additional, Laucou, Valérie, additional, Bacilieri, Roberto, additional, Röckel, Franco, additional, Guan, Pingyin, additional, Jung, Andreas, additional, Riemann, Michael, additional, Ujmajuridze, Levan, additional, Zakalashvili, Tekle, additional, Maghradze, David, additional, Höhn, Maria, additional, Jahnke, Gizella, additional, Kiss, Erzsébet, additional, Deák, Tamás, additional, Rahimi, Oshrit, additional, Hübner, Sariel, additional, Grassi, Fabrizio, additional, Mercati, Francesco, additional, Sunseri, Francesco, additional, Eiras-Dias, José, additional, Dumitru, Anamaria Mirabela, additional, Carrasco, David, additional, Rodriguez-Izquierdo, Alberto, additional, Muñoz, Gregorio, additional, Uysal, Tamer, additional, Özer, Cengiz, additional, Kazan, Kemal, additional, Xu, Meilong, additional, Wang, Yunyue, additional, Zhu, Shusheng, additional, Lu, Jiang, additional, Zhao, Maoxiang, additional, Wang, Lei, additional, Jiu, Songtao, additional, Zhang, Ying, additional, Sun, Lei, additional, Yang, Huanming, additional, Weiss, Ehud, additional, Wang, Shiping, additional, Zhu, Youyong, additional, Li, Shaohua, additional, Sheng, Jun, additional, and Chen, Wei, additional

Published
2023
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27. Viticultural Suitability Analysis Based on Multi-Source Data Highlights Climate-Change-Induced Decrease in Potential Suitable Areas: A Case Analysis in Ningxia, China

Author

Bai, Huiqing, primary, Sun, Zhongxiang, additional, Yao, Xuenan, additional, Kong, Junhua, additional, Wang, Yongjian, additional, Zhang, Xiaoyu, additional, Chen, Weiping, additional, Fan, Peige, additional, Li, Shaohua, additional, Liang, Zhenchang, additional, and Dai, Zhanwu, additional

Published
2022
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28. A genome for Cissus illustrates features underlying the evolutionary success in dry savannas

Author

Xin, Haiping, primary, Wang, Yi, additional, Li, Qingyun, additional, Wan, Tao, additional, Hou, Yujun, additional, Liu, Yuanshuang, additional, Gichuki, Duncan, additional, Zhou, Huimin, additional, Zhu, Zhenfei, additional, Xu, Chen, additional, Zhou, Yadong, additional, Liu, Zhiming, additional, Li, Rongjun, additional, Liu, Bing, additional, Lu, Limin, additional, Jiang, Hongsheng, additional, Zhang, Jisen, additional, Wan, Jun-Nan, additional, Aryal, Rishi, additional, Hu, Guangwan, additional, Chen, Zhi-Duan, additional, Gituru, Robert, additional, Liang, Zhenchang, additional, Wen, Jun, additional, and Wang, Qingfeng, additional

Published
2022
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29. A chromosome‐level genome of Syringa oblata provides new insights into chromosome formation in Oleaceae and evolutionary history of lilacs

Author

Wang, Yi, primary, Lu, Limin, additional, Li, Jingrui, additional, Li, Huayang, additional, You, Yichen, additional, Zang, Shuying, additional, Zhang, Yongqing, additional, Ye, Jianfei, additional, Lv, Zemin, additional, Zhang, Zhaoyu, additional, Qin, Yongsheng, additional, Zhang, Hongling, additional, Xia, Fei, additional, Li, Hui, additional, Zhang, Huijin, additional, Fan, Peige, additional, Shi, Lei, additional, Liang, Zhenchang, additional, and Cui, Hongxia, additional

Published
2022
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31. Additional file 1 of Hormone biosynthesis and metabolism members of 2OGD superfamily are involved in berry development and respond to MeJA and ABA treatment of Vitis vinifera L

Author

Gao, Yingying, Wang, Xiaochen, Liu, Xianju, and Liang, Zhenchang

Abstract

Additional file 1: Supplementary Figure S1. Evolutionary tree of 163 Vv2OGDs and 130 At2OGDs. The branches in different clades are distinguished in different color. DOXA, DOXB and DOXC class are indicated at the outer arc, and different clades are indicated at the inside arc. Supplementary Figure S2. Sequence alignment of 37 Vv2OGD-H proteins. VvLBO3 is too long (701aa) to display, it is cut partly in C terminal. The locations of His-Xaa-Asp/Glu-(Xaa)n-His [HX(D/E)XnH] and Arg-Xaa-Ser/Thr (RxS/T) motif are highlighted in red and orange boxes respectively. Supplementary Figure S3. Motif analysis of 31 At2OGD-H and 37 Vv2OGD-H members. Phylogenetic tree (left) of 31 At2OGD-Hs and 37 Vv2OGD-Hs and their motif analysis (right). Different motifs are represented by different colored block with numbers in it. Sequence of each motif is listed in Suppl. Table S3. Supplementary Table S1. Detailed information of 163 members of Vv2OGD superfamily. Supplementary Table S2. Primers used in this study. Supplementary Table S3. Sequence and details of each motif in Suppl. Fig. S3.

Published
2022
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32. Natural variations of HSFA2 enhance thermotolerance in grapevine.

Author

Liu, Xinna, Chen, Haiyang, Li, Shenchang, Lecourieux, David, Duan, Wei, Fan, Peige, Liang, Zhenchang, and Wang, Lijun

Subjects
GRAPES, GENETIC variation, CROP development, VITIS vinifera, CROP growth, TRANSCRIPTION factors
Abstract

Heat stress limits growth and development of crops including grapevine which is a popular fruit in the world. Genetic variability in crops thermotolerance is not well understood. We identified and characterized heat stress transcription factor HSFA2 in heat sensitive Vitis vinifera 'Jingxiu' (named as VvHSFA2) and heat tolerant Vitis davidii 'Tangwei' (named as VdHSFA2). The transcriptional activation activities of VdHSFA2 are higher than VvHSFA2, the variation of single amino acid (Thr315Ile) in AHA1 motif leads to the difference of transcription activities between VdHSFA2 and VvHSFA2. Based on 41 Vitis germplasms, we found that HSFA2 is differentiated at coding region among heat sensitive V. vinifera, and heat tolerant Vitis davidii and Vitis quinquangularis. Genetic evidence demonstrates VdHSFA2 and VvHSFA2 are positive regulators in grape thermotolerance, and the former can confer higher thermotolerance than the latter. Moreover, VdHSFA2 can regulate more target genes than VvHSFA2. As a target gene of both VdHSFA2 and VvHSFA2, overexpression of MBF1c enhanced the grape thermotolerance whereas dysfunction of MBF1c resulted in thermosensitive phenotype. Together, our results revealed that VdHSFA2 confers higher thermotolerance than VvHSFA2, and MBF1c acts as their target gene to induce thermotolerance. The VdHSFA2 may be adopted for molecular breeding in grape thermotolerance. [ABSTRACT FROM AUTHOR]

Published
2023
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36. Comparative Metabolomics Analysis of Dormancy Buds During Cold Accumulation between Cold-Sensitive Grapevine (Vitis Vinifera) and Cold-Hardy Grapevine (Vitis Amurensis)

Author

Xin, Haiping, primary, Li, Qingyun, additional, Zhou, Huimin, additional, Chai, Fengmei, additional, Wang, Zemin, additional, Fang, Linchuan, additional, Duan, Wei, additional, Fan, Peige, additional, Liang, Zhenchang, additional, Li, Shaohua, additional, and Wang, Qingfeng, additional

Published
2022
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37. A genome for Cissus illustrates features underlying its evolutionary success in dry savannas

Author

Xin, Haiping, primary, Wang, Yi, additional, Li, Qingyun, additional, Wan, Tao, additional, Hou, Yujun, additional, Liu, Yuanshuang, additional, Gichuki, Duncan Kiragu, additional, Zhou, Huimin, additional, Zhu, Zhenfei, additional, Xu, Chen, additional, Zhou, Yadong, additional, Liu, Zhiming, additional, Li, Rongjun, additional, Liu, Bing, additional, Lu, Limin, additional, Jiang, Hongsheng, additional, Zhang, Jisen, additional, Wan, Junnan, additional, Aryal, Rishi, additional, Hu, Guangwan, additional, Chen, Zhiduan, additional, Gituru, Robert Wahiti, additional, Liang, Zhenchang, additional, Wen, Jun, additional, and Wang, Qingfeng, additional

Published
2022
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40. Biography of Vitisgenomics: recent advances and prospective

Author

Wang, Yi, Ding, Kangyi, Li, Huayang, Kuang, Yangfu, and Liang, Zhenchang

Abstract

The grape genome is the basis for grape studies and breeding, and is also important for grape industries. In the last two decades, more than 44 grape genomes have been sequenced. Based on these genomes, researchers have made substantial progress in understanding the mechanism of biotic and abiotic resistance, berry quality formation, and breeding strategies. In addition, this work has provided essential data for future pangenome analyses. Apart from de novoassembled genomes, more than six whole-genome sequencing projects have provided datasets comprising almost 5000 accessions. Based on these datasets, researchers have explored the domestication and origins of the grape and clarified the gene flow that occurred during its dispersed history. Moreover, genome-wide association studies and other methods have been used to identify more than 900 genes related to resistance, quality, and developmental phases of grape. These findings have benefited grape studies and provide some basis for smart genomic selection breeding. Moreover, the grape genome has played a great role in grape studies and the grape industry, and the importance of genomics will increase sharply in the future.

Published
2024
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41. Characterization of Chromatin Accessibility and Gene Expression upon Cold Stress Reveals that the RAV1 Transcription Factor Functions in Cold Response in Vitis Amurensis.

Author

Ren, Chong, Li, Huayang, Wang, Zemin, Dai, Zhanwu, Lecourieux, Fatma, Kuang, Yangfu, Xin, Haiping, Li, Shaohua, and Liang, Zhenchang

Subjects
GENE expression, TRANSCRIPTION factors, GRAPES, GENE regulatory networks, PLANT cell walls, PHYSIOLOGICAL effects of cold temperatures
Abstract

Cold tolerance is regulated by a variety of transcription factors (TFs) and their target genes. Except for the well-characterized C-repeat binding factors (CBFs)-dependent transcriptional cascade, the mechanisms of cold tolerance mediated by other transcriptional regulatory networks are still largely unknown. Here, we used the assay for transposase-accessible chromatin with sequencing (ATAC-seq) and RNA-seq to identify cold responsive TFs in Vitis amurensis , a grape species with high cold hardiness. Nine TFs, including CBF4, RAV1 and ERF104, were identified after cold treatment. Weighted gene co-expression network analysis (WGCNA) and gene ontology (GO) analysis revealed that these TFs may regulate cold response through different pathways. As a prime candidate TF, overexpression of VaRAV1 in grape cells improved its cold tolerance. The transgenic cells exhibited low electrolyte leakage and malondialdehyde content and high peroxidase activity. Moreover, the TF gene TCP8 and a gene involving in homogalacturonan biosynthesis were found to be regulated by VaRAV1, suggesting that the contribution of VaRAV1 to cold tolerance may be achieved by enhancing the stability of cell membrane and regulating the expression of target genes involved in plant cell wall composition. Our work provides novel insights into plant response to cold stress and demonstrates the utility of ATAC-seq and RNA-seq for the rapid identification of TFs in response to cold stress in grapevine. VaRAV1 may play an important role in adaption to cold stress. [ABSTRACT FROM AUTHOR]

Published
2021
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42. Basic leucine zipper gene VvbZIP61is expressed at a quantitative trait locus for high monoterpene content in grape berries

Author

Zhang, Yuyu, Liu, Cuixia, Liu, Xianju, Wang, Zemin, Wang, Yi, Zhong, Gan-yuan, Li, Shaohua, Dai, Zhanwu, Liang, Zhenchang, and Fan, Peige

Abstract

The widely appreciated muscat flavor of grapes and wine is mainly attributable to the monoterpenes that accumulate in ripe grape berries. To identify quantitative trait loci (QTL) for grape berry monoterpene content, an F1mapping population was constructed by a cross between two grapevine genotypes, one with neutral aroma berries (cv. ‘Beifeng’) and the other with a pronounced muscat aroma (elite Vitis viniferaline ‘3–34’). A high-density genetic linkage map spanning 1563.7 cM was constructed using 3332 SNP markers that were assigned to 19 linkage groups. Monoterpenes were extracted from the berry of the F1progeny, then identified and quantified by gas chromatography–mass spectrometry. Twelve stable QTLs associated with the amounts of 11 monoterpenes in berries were thus identified. In parallel, the levels of RNA in berries from 34 diverse cultivars were estimated by RNA sequencing and compared to the monoterpene content of the berries. The expression of five genes mapping to stable QTLs correlated well with the monoterpene content of berries. These genes, including the basic leucine zipper VvbZIP61gene on chromosome 12, are therefore considered as potentially being involved in monoterpene metabolism. Overexpression of VvbZIP61in Vitis amurensiscallus through Agrobacterium-mediated transformation significantly increased the accumulation of several monoterpenes in the callus, including nerol, linalool, geranial, geraniol, β-myrcene, and D-limonene. It is hypothesized that VvbZIP61expression acts to increase muscat flavor in grapes. These results advance our understanding of the genetic control of monoterpene biosynthesis in grapes and provide important information for the marker-assisted selection of aroma compounds in grape breeding.

Published
2023
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43. A genome for Cissusillustrates features underlying its evolutionary success in dry savannas

Author

Xin, Haiping, Wang, Yi, Li, Qingyun, Wan, Tao, Hou, Yujun, Liu, Yuanshuang, Gichuki, Duncan Kiragu, Zhou, Huimin, Zhu, Zhenfei, Xu, Chen, Zhou, Yadong, Liu, Zhiming, Li, Rongjun, Liu, Bing, Lu, Limin, Jiang, Hongsheng, Zhang, Jisen, Wan, Junnan, Aryal, Rishi, Hu, Guangwan, Chen, Zhiduan, Gituru, Robert Wahiti, Liang, Zhenchang, Wen, Jun, and Wang, Qingfeng

Abstract

Cissusis the largest genus in Vitaceae and is mainly distributed in the tropics and subtropics. Crassulacean acid metabolism (CAM), a photosynthetic adaptation to the occurrence of succulent leaves or stems, indicates that convergent evolution occurred in response to drought stress during species radiation. Here we provide the chromosomal level assembly of Cissus rotundifolia(an endemic species in Eastern Africa) and a genome-wide comparison with grape to understand genome divergence within an ancient eudicot family. Extensive transcriptome data were produced to illustrate the genetics underpinning C. rotundifolia’s ecological adaption to seasonal aridity. The modern karyotype and smaller genome of C. rotundifolia(n= 12, 350.69 Mb/1C), which lack further whole-genome duplication, were mainly derived from gross chromosomal rearrangements such as fusions and segmental duplications, and were sculpted by a very recent burst of retrotransposon activity. Bias in local gene amplification contributed to its remarkable functional divergence from grape, and the specific proliferated genes associated with abiotic and biotic responses (e.g. HSP-20, NBS-LRR) enabled C. rotundifoliato survive in a hostile environment. Reorganization of existing enzymes of CAM characterized as diurnal expression patterns of relevant genes further confer the ability to thrive in dry savannas.

Published
2022
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44. Dual domestications and origin of traits in grapevine evolution

Author

Yang Dong, Shengchang Duan, Qiuju Xia, Zhenchang Liang, Xiao Dong, Kristine Margaryan, Mirza Musayev, Svitlana Goryslavets, Goran Zdunić, Pierre-François Bert, Thierry Lacombe, Erika Maul, Peter Nick, Kakha Bitskinashvili, György Dénes Bisztray, Elyashiv Drori, Gabriella De Lorenzis, Jorge Cunha, Carmen Florentina Popescu, Rosa Arroyo-Garcia, Claire Arnold, Ali Ergül, Yifan Zhu, Chao Ma, Shufen Wang, Siqi Liu, Liu Tang, Chunping Wang, Dawei Li, Yunbing Pan, Jingxian Li, Ling Yang, Xuzhen Li, Guisheng Xiang, Zijiang Yang, Baozheng Chen, Zhanwu Dai, Yi Wang, Arsen Arakelyan, Varis Kuliyev, Gennady Spotar, Nabil Girollet, Serge Delrot, Nathalie Ollat, Patrice This, Cécile Marchal, Gautier Sarah, Valérie Laucou, Roberto Bacilieri, Franco Röckel, Pingyin Guan, Andreas Jung, Michael Riemann, Levan Ujmajuridze, Tekle Zakalashvili, David Maghradze, Maria Höhn, Gizella Jahnke, Erzsébet Kiss, Tamás Deák, Oshrit Rahimi, Sariel Hübner, Fabrizio Grassi, Francesco Mercati, Francesco Sunseri, José Eiras-Dias, Anamaria Mirabela Dumitru, David Carrasco, Alberto Rodriguez-Izquierdo, Gregorio Muñoz, Tamer Uysal, Cengiz Özer, Kemal Kazan, Meilong Xu, Yunyue Wang, Shusheng Zhu, Jiang Lu, Maoxiang Zhao, Lei Wang, Songtao Jiu, Ying Zhang, Lei Sun, Huanming Yang, Ehud Weiss, Shiping Wang, Youyong Zhu, Shaohua Li, Jun Sheng, Wei Chen, Duan, Shengchang, Xia, Qiuju, Liang, Zhenchang, Margaryan, Kristine, Musayev, Mirza, Goryslavets, Svitlana, Zdunić, Goran, Bert, Pierre-François, Lacombe, Thierry, Maul, Erika, Bitskinashvili, Kakha, Bisztray, György Dénes, Drori, Elyashiv, De Lorenzis, Gabriella, Popescu, Carmen Florentina, Arroyo García, Rosa Adela, Chen, Baozheng, Dai, Zhanwu, Spotar, Gennady, Girollet, Nabil, Delrot, Serge, Ollat, Nathalie, This, Patrice, Sarah, Gautier, Laucou, Valérie, Bacilieri, Roberto, Röckel, Franco, Guan, Pingyin, Riemann, Michael, Ujmajuridze, Levan, Zakalashvili, Tekle, Höhn, Maria, Jahnke, Gizella, Kiss, Erzsébet, Deák, Tamás, Rahimi, Oshrit, Hübner, Sariel, Grassi, Fabrizio, Mercati, Francesco, Eiras-Dias, José, Dumitru, Anamaria Mirabela, Carrasco, David, Rodriguez-Izquierdo, Alberto, Muñoz, Gregorio, Uysal, Tamer, Özer, Cengiz, Kazan, Kemal, Wang, Yunyue, Zhao, Maoxiang, Weiss, Ehud, Dong, Y, Duan, S, Xia, Q, Liang, Z, Dong, X, Margaryan, K, Musayev, M, Goryslavets, S, Zdunić, G, Bert, P, Lacombe, T, Maul, E, Nick, P, Bitskinashvili, K, Bisztray, G, Drori, E, De Lorenzis, G, Cunha, J, Popescu, C, Arroyo-Garcia, R, Arnold, C, Ergül, A, Zhu, Y, Ma, C, Wang, S, Liu, S, Tang, L, Wang, C, Li, D, Pan, Y, Li, J, Yang, L, Li, X, Xiang, G, Yang, Z, Chen, B, Dai, Z, Wang, Y, Arakelyan, A, Kuliyev, V, Spotar, G, Girollet, N, Delrot, S, Ollat, N, This, P, Marchal, C, Sarah, G, Laucou, V, Bacilieri, R, Röckel, F, Guan, P, Jung, A, Riemann, M, Ujmajuridze, L, Zakalashvili, T, Maghradze, D, Höhn, M, Jahnke, G, Kiss, E, Deák, T, Rahimi, O, Hübner, S, Grassi, F, Mercati, F, Sunseri, F, Eiras-Dias, J, Dumitru, A, Carrasco, D, Rodriguez-Izquierdo, A, Muñoz, G, Uysal, T, Özer, C, Kazan, K, Xu, M, Zhu, S, Lu, J, Zhao, M, Wang, L, Jiu, S, Zhang, Y, Sun, L, Yang, H, Weiss, E, Li, S, Sheng, J, and Chen, W

Subjects
Domestication, Ecotype, Phenotype, Multidisciplinary, Acclimatization, Grapes, domestication, origin, Genetics, Humans, genetic diversity, genomic history, accessions, holocene, insights, Agriculture, Vitis, Asia Western, Biological Evolution
Abstract

We elucidate grapevine evolution and domestication histories with 3525 cultivated and wild accessions worldwide. In the Pleistocene, harsh climate drove the separation of wild grape ecotypes caused by continuous habitat fragmentation. Then, domestication occurred concurrently about 11,000 years ago in Western Asia and the Caucasus to yield table and wine grapevines. The Western Asia domesticates dispersed into Europe with early farmers, introgressed with ancient wild western ecotypes, and subsequently diversified along human migration trails into muscat and unique western wine grape ancestries by the late Neolithic. Analyses of domestication traits also reveal new insights into selection for berry palatability, hermaphroditism, muscat flavor, and berry skin color. These data demonstrate the role of the grapevines in the early inception of agriculture across Eurasia.

Published
2023

45. Basic leucine zipper gene VvbZIP61 is expressed at a quantitative trait locus for high monoterpene content in grape berries.

Author

Zhang Y, Liu C, Liu X, Wang Z, Wang Y, Zhong GY, Li S, Dai Z, Liang Z, and Fan P

Abstract

The widely appreciated muscat flavor of grapes and wine is mainly attributable to the monoterpenes that accumulate in ripe grape berries. To identify quantitative trait loci (QTL) for grape berry monoterpene content, an F 1 mapping population was constructed by a cross between two grapevine genotypes, one with neutral aroma berries (cv. 'Beifeng') and the other with a pronounced muscat aroma (elite Vitis vinifera line '3-34'). A high-density genetic linkage map spanning 1563.7 cM was constructed using 3332 SNP markers that were assigned to 19 linkage groups. Monoterpenes were extracted from the berry of the F 1 progeny, then identified and quantified by gas chromatography-mass spectrometry. Twelve stable QTLs associated with the amounts of 11 monoterpenes in berries were thus identified. In parallel, the levels of RNA in berries from 34 diverse cultivars were estimated by RNA sequencing and compared to the monoterpene content of the berries. The expression of five genes mapping to stable QTLs correlated well with the monoterpene content of berries. These genes, including the basic leucine zipper VvbZIP61 gene on chromosome 12, are therefore considered as potentially being involved in monoterpene metabolism. Overexpression of VvbZIP61 in Vitis amurensis callus through Agrobacterium-mediated transformation significantly increased the accumulation of several monoterpenes in the callus, including nerol, linalool, geranial, geraniol, β-myrcene, and D-limonene. It is hypothesized that VvbZIP61 expression acts to increase muscat flavor in grapes. These results advance our understanding of the genetic control of monoterpene biosynthesis in grapes and provide important information for the marker-assisted selection of aroma compounds in grape breeding., Competing Interests: None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.)

Published
2023
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