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In this paper, the water level coseismic response characteristics of Sichuan fluid network to the three iarge earthquakes, the 2022 Lushan MS6. 1, the 2022 Maerkang MS6. 0 and the 2022 Luding MS6. 8 earthquakes, are analyzed statistically. The results show that the three strong earthquakes induced different degrees of water level coseismic response in most areas of Sichuan. The coseismic responses amplitude of water level induced by the three earthquakes are significantly correlated with the earthquake magnitude, while not significantly correlated with well-epicenter distances. In a large range, the coseismic response amplitude of water level are influenced by the aquifer lithology, geological structure, and wellbore conditions. The coseismic response types of water level are mainly affected by the well-epicenter distances, and little related to the wellbore-depth. For the 2022 Luding MS6. 8 earthquake, the reason for the large difference in the coseismic response amplitude of water level of wells Chuan-20, Chuan-49 and Chuan-50 may be the different wellbore bearing capacity. Due to the effects from the aquifer lithology, the location in the fault zone and the wellbore bearing capacity, the coseismic response amplitude of the water level of wells Chuan-46, Chuan-47 and Chuan-48, being close to each other in the same fault zone, are different in the three strong earthquakes. In the 2022 Lushan MS6. 1 earthquake, the coseismic response types of water level between wells Chuan-46 and Chuan-47 are different, which may be related to the different main control factors of dynamic or static stress on the wells water level. The statistical and analytical results of water level coseismic response are affected by the sampling rate of instruments. The analysis results of water level coseismic response, using minute water level data, may not be accurate enough. The seconds water level data can provide more accurate and rich water seismic waves information for the detailed study of the water level coseismic response characteristics and mechanisms. [ABSTRACT FROM AUTHOR]

Lidocaine is the most frequently applied local infiltration anesthetic agent for treating tendinopathies. However, studies have discovered lidocaine to negatively affect tendon healing. In the current study, the molecular mechanisms and effects of lidocaine on tenocyte migration were evaluated. We treated tenocytes intrinsic to the Achilles tendons of Sprague–Dawley rats with lidocaine. The migration ability of cells was analyzed using electric cell‐substrate impedance sensing (ECIS) and scratch wound assay. We then used a microscope to evaluate the cell spread. We assessed filamentous actin (F‐actin) cytoskeleton formation through immunofluorescence staining. In addition, we used Western blot analysis to analyze the expression of phospho‐focal adhesion kinase (FAK), FAK, phospho‐paxillin, paxillin, and F‐actin. We discovered that lidocaine had an inhibitory effect on the migration of tenocytes in the scratch wound assay and on the ECIS chip. Lidocaine treatment suppressed cell spreading and changed the cell morphology and F‐actin distribution. Lidocaine reduced F‐actin formation in the tenocyte during cell spreading; furthermore, it inhibited phospho‐FAK, F‐actin, and phospho‐paxillin expression in the tenocytes. Our study revealed that lidocaine inhibits the spread and migration of tenocytes. The molecular mechanism potentially underlying this effect is downregulation of F‐actin, phospho‐FAK, and phospho‐paxillin expression when cells are treated with lidocaine. [ABSTRACT FROM AUTHOR]

Muscle injuries are common among athletes and often treated with platelet‐rich plasma (PRP). However, whether the leukocyte concentration affects the efficacy of PRP in treating muscle injuries remains unclear. This study investigated the effects of leukocyte‐poor platelet‐rich plasma (LP‐PRP) and leukocyte‐rich platelet‐rich plasma (LR‐PRP) on myoblast proliferation and the molecular mechanisms underlying these effects. Myoblasts were treated with 0.5% LP‐PRP, 0.5% LR‐PRP, 1% LP‐PRP, or 1% LR‐PRP for 24 h. The gene expression of the LP‐PRP‐ and LR‐PRP–treated myoblasts was determined using RNA sequencing analysis. Cell proliferation was evaluated using an bromodeoxyuridine (BrdU) assay, and cell cycle progression was assessed through flow cytometry. The expression of cyclin A, cyclin‐dependent kinase 1 (cdk1), and cdk2 was examined using Western blotting. The expression of myoblast determination protein 1 (MyoD1) was examined through Western blotting and immunofluorescence staining. The LP‐PRP and LR‐PRP both promoted the proliferation of myoblasts and increased differential gene expression of myoblasts. Moreover, the LP‐PRP and LR‐PRP substantially upregulated the expression of cyclin A, cdk1, and cdk2. MyoD1 expression was induced in the LP‐PRP and LR‐PRP–treated myoblasts. Our results corroborate the finding that LP‐PRP and LR‐PRP have similar positive effects on myoblast proliferation and MyoD1 expression. [ABSTRACT FROM AUTHOR]

To study the complications of ultrasound-guided radiofrequency ablation (RFA) in chronic kidney disease (CKD) patients undergoing renal replacement therapy with secondary hyperparathyroidism (SHPT). This retrospective study reviewed the clinical data, including general information, examination results, treatment times, time interval, and postoperative complications, of 103 SHPT patients who received ultrasound-guided RFA treatment from July 2017 to January 2021. Of 103 patients, 52 required two sessions of RFA within a month. The incidence of recurrent laryngeal nerve injury at the second treatment was significantly higher than that at the first treatment (first session vs. second session, 5.77% vs. 21.15%; p =.021). Of all the enrolled 103 patients, 27 suffered complications after the first session of RFA. When we separated patients into complications group and non-complication group, we detected more ablated nodules in the complications group (Z = −2.222; p =.0026). Subgroup analysis further showed that the patients in the severe hypocalcemia group were younger (p =.005), had more ablated nodules (p =.003) and higher blood phosphorus (p =.012) and alkaline phosphatase (ALP) levels (p =.002). Univariate analysis showed that age, serum phosphorus, ALP, and number of ablated nodules were associated with a higher risk of severe hypocalcemia after the first session of RFA. An interval of more than 1 month between two treatments may help to avoid recurrent laryngeal nerve injury. Age, serum phosphorus, ALP, and number of ablated nodules were associated with a higher risk of severe hypocalcemia after the first session of RFA. [ABSTRACT FROM AUTHOR]

• The peak time delay was an independent predictor of good collaterals in AIS patients. • The peak time delay was an independent predictor of good outcomes in AIS patients. • Tissue fate were moderately positively correlated with peak density decrease. Collateral status is a pivotal determinant of clinical outcomes in acute ischemic stroke (AIS); however, its evaluation can be challenging. We investigated the predictive value of CT perfusion (CTP) derived time and density alterations versus CTP for collateral status prediction in AIS. Consecutive patients with anterior circulation occlusion within 24 h were retrospectively included. Time-density curves of the CTP specified ischemic core, penumbra, and the corresponding contralateral unaffected brain were obtained. The collateral status was dichotomised into robust (4–5 scores) and poor (0–3 scores) using multiphase collateral scoring, as described by Menon et al.. Receiver operating characteristic curves and multivariable regression analysis were performed to assess the predictive ability of CTP-designated tissue time and density alterations, CTP for robust collaterals, and favourable outcomes (mRS score of 0–2 at 90 days). One-hundred patients (median age, 68 years; interquartile range, 57–80 years; 61 men) were included. A smaller ischemic core, shorter peak time delay, lower peak density decrease, lower cerebral blood volume ratio, and cerebral blood flow ratio in the CTP specified ischemic core were significantly associated with robust collaterals (P FDR ≤ 0.004). The peak time delay demonstrated the highest diagnostic value (AUC, 0.74; P < 0.001) with 66.7 % sensitivity and 73.7 % specificity. Furthermore, the peak time delay of less than 8.5 s was an independent predictor of robust collaterals and favourable clinical outcomes. Robust collateral status was significantly associated with the peak time delay in the ischemic core. It is a promising image marker for predicting collateral status and functional outcomes in AIS. [ABSTRACT FROM AUTHOR]

There are health benefits from consuming cruciferous vegetables that release indole-3-carbinol (I3C), but the in vivo transformation of I3C-related indoles remains underinvestigated. Here we detail the post-ingestion conversion of I3C into antitumor agents, 2-(indol-3-ylmethyl)-3,3′-diindolylmethane (LTr1) and 3,3′-diindolylmethane (DIM), by conceptualizing and materializing the reaction flux derailing (RFD) approach as a means of unraveling these stepwise transformations to be non-enzymatic but pH-dependent and gut microbe-sensitive. In the upper (or acidic) gastrointestinal tract, LTr1 is generated through Michael addition of 3-methyleneindolium (3MI, derived in situ from I3C) to DIM produced from I3C via the formaldehyde-releasing (major) and CO2-liberating (minor) pathways. In the large intestine, 'endogenous' I3C and DIM can form, respectively, from couplings of formaldehyde with one and two molecules of indole (a tryptophan catabolite). Acid-producing gut bacteria such as Lactobacillus acidophilus facilitate the H+-promotable steps. This work updates our understanding of the merits of I3C consumption and identifies LTr1 as a drug candidate. [ABSTRACT FROM AUTHOR]

Background: Brain structure injury was presented in acute lymphoblastic leukemia (ALL) after treatment; however, its alterations in new-onset stage are still unclear. We aim to explore white matter (WM) and grey matter (GM) alterations using surface-based morphometry (SBM) and tract-based spatial statistics (TBSS) in new-onset pediatric ALL., Methods: Thirty-five ALL and 33 typically developing (TD) children were prospectively recruited and underwent three-dimensional T1-weighted and diffusion tensor (DTI) imaging. DTI metrics, cortical GM features, and deep GM nuclei volume were compared between groups differences., Results: In ALL, the only increased FA in the body of corpus callosum (P FWE-corrected  = 0.023) and left superior corona radiata (P FWE-corrected  = 0.045) were presented. Relative to TDs, pediatric ALL presented a significant decrease in cortical surface area (CSA), thickness (CT), and volume in orbital gyri, supramarginal gyrus, middle temporal gyrus, and superior temporal gyrus (all CWP = 0.01). Additionally, increased CT and CSA were found in lingual gyrus and left sulcus intermedius primus, respectively (all CWP = 0.01). Smaller volumes in pediatric ALL were observed in bilateral thalamus, caudate, hippocampus, and right putamen (P FDR-corrected  < 0.05)., Conclusion: Widespread brain structural abnormalities were found in new-onset pediatric ALL, which suggest disease itself can cause brain structural injury., Impact: This study revealed the altered white matter integrity and gray matter morphology characteristics in childhood acute lymphoblastic leukemia on new-onset stage. It is suggested that there may be structural impairment before chemotherapy. MRI is a sensitive way for early detection on brain structural damage in childhood acute lymphoblastic leukemia., (© 2024. The Author(s), under exclusive licence to the International Pediatric Research Foundation, Inc.)